This page has only limited features, please log in for full access.
Carbon quantum dots (CQDs) have recently emerged as innovative theranostic nanomaterials, enabling fast and effective diagnosis and treatment. In this study, a facile hydrothermal approach for N-doped biomass-derived CQDs preparation from Citrus clementina peel and amino acids glycine (Gly) and arginine (Arg) has been presented. The gradual increase in the N-dopant (amino acids) nitrogen content increased the quantum yield of synthesized CQDs. The prepared CQDs exhibited good biocompatibility, stability in aqueous, and high ionic strength media, similar optical properties, while differences were observed regarding the structural and chemical diversity, and biological and antioxidant activity. The antiproliferative effect of [email protected] against pancreatic cancer cell lines (CFPAC-1) was observed. At the same time, [email protected] has demonstrated the highest quantum yield and antioxidant activity by DPPH scavenging radical method of 81.39 ± 0.39% and has been further used for the ion sensing and cellular imaging of cancer cells. The obtained results have demonstrated selective response toward Fe3+ detection, with linear response ranging from 7.0 µmol dm−3 to 50.0 µmol dm−3 with R2 = 0.9931 and limit of detection (LOD) of 4.57 ± 0.27 µmol dm−3. This research could be a good example of sustainable biomass waste utilization with potential for biomedical analysis and ion sensing applications.
Silvija Šafranko; Anamarija Stanković; Sugato Hajra; Hoe-Joon Kim; Ivica Strelec; Maja Dutour-Sikirić; Igor Weber; Maja Herak Bosnar; Petra Grbčić; Sandra Kraljević Pavelić; Aleksandar Széchenyi; Yogendra Kumar Mishra; Igor Jerković; Stela Jokić. Preparation of Multifunctional N-Doped Carbon Quantum Dots from Citrus clementina Peel: Investigating Targeted Pharmacological Activities and the Potential Application for Fe3+ Sensing. Pharmaceuticals 2021, 14, 857 .
AMA StyleSilvija Šafranko, Anamarija Stanković, Sugato Hajra, Hoe-Joon Kim, Ivica Strelec, Maja Dutour-Sikirić, Igor Weber, Maja Herak Bosnar, Petra Grbčić, Sandra Kraljević Pavelić, Aleksandar Széchenyi, Yogendra Kumar Mishra, Igor Jerković, Stela Jokić. Preparation of Multifunctional N-Doped Carbon Quantum Dots from Citrus clementina Peel: Investigating Targeted Pharmacological Activities and the Potential Application for Fe3+ Sensing. Pharmaceuticals. 2021; 14 (9):857.
Chicago/Turabian StyleSilvija Šafranko; Anamarija Stanković; Sugato Hajra; Hoe-Joon Kim; Ivica Strelec; Maja Dutour-Sikirić; Igor Weber; Maja Herak Bosnar; Petra Grbčić; Sandra Kraljević Pavelić; Aleksandar Széchenyi; Yogendra Kumar Mishra; Igor Jerković; Stela Jokić. 2021. "Preparation of Multifunctional N-Doped Carbon Quantum Dots from Citrus clementina Peel: Investigating Targeted Pharmacological Activities and the Potential Application for Fe3+ Sensing." Pharmaceuticals 14, no. 9: 857.
The search for an efficient drug or inhibitor in the formation process of kidney stones has been a promising research topic towards reducing the risks of the formation of disease. However, several challenges have been faced in investigating the most common constituents of kidney stones, calcium oxalate and its hydrate forms (COM, COD and COT). This study focuses on the preparation and structural characterization (TG, XRD, FTIR, SEM) of calcium oxalate hydrates in the presence of gallic acid (GA) and by varying operating parameters such as temperature (25 °C, 36.5 °C and 48 °C), pH (5.6, 6.5 and 7.5) and amount of added GA (ranging from 100 mg to 1000 mg). Response surface methodology was applied in order to evaluate the effects of operating parameters in the formation of COM and COD, and for the process optimization towards maximizing their content in samples. The results indicated that GA inhibited the formation of COM (0–100%) and promoted the formation of COD (0 ≤ 99%), while a medium pH and the amount of added GA showed a significant effect in the process of COD formation. In order to investigate the interactions established in the formation process and the possible adsorption between GA and the formed crystals, electrochemical measurements were performed.
Silvija Šafranko; Sara Goman; Dominik Goman; Stela Jokić; Ida Delač Marion; Nives Matijaković Mlinarić; Atiđa Selmani; Martina Medvidović-Kosanović; Anamarija Stanković. Calcium Oxalate and Gallic Acid: Structural Characterization and Process Optimization toward Obtaining High Contents of Calcium Oxalate Monohydrate and Dihydrate. Crystals 2021, 11, 954 .
AMA StyleSilvija Šafranko, Sara Goman, Dominik Goman, Stela Jokić, Ida Delač Marion, Nives Matijaković Mlinarić, Atiđa Selmani, Martina Medvidović-Kosanović, Anamarija Stanković. Calcium Oxalate and Gallic Acid: Structural Characterization and Process Optimization toward Obtaining High Contents of Calcium Oxalate Monohydrate and Dihydrate. Crystals. 2021; 11 (8):954.
Chicago/Turabian StyleSilvija Šafranko; Sara Goman; Dominik Goman; Stela Jokić; Ida Delač Marion; Nives Matijaković Mlinarić; Atiđa Selmani; Martina Medvidović-Kosanović; Anamarija Stanković. 2021. "Calcium Oxalate and Gallic Acid: Structural Characterization and Process Optimization toward Obtaining High Contents of Calcium Oxalate Monohydrate and Dihydrate." Crystals 11, no. 8: 954.
This study intends to valorize by-products of the industrial processing of tobacco to obtain nicotine and phenolics as value-added compounds. Three influential parameters of the microwave-assisted extraction-MAE (temperature, treatment time, and solvent/solid ratio) were studied for the optimization of the extraction protocol for tobacco leaves and three types of waste—scrap, dust, and midrib, respectively. Nicotine was the dominant bioactive compound in all extracts, ranging from 1.512 to 5.480% in leaves, 1.886 to 3.709% in scrap, 2.628 to 4.840% dust, and 0.867 to 1.783% in midrib extracts. Five phenolic compounds were identified and quantified, predominated by chlorogenic acid and rutin. Additionally, total phenol content and antioxidant activity were determined using spectrophotometric assays. Optimization was performed in two aspects: to obtain a maximum extraction yield with minimum nicotine content and to obtain a maximum extraction yield with maximum nicotine content. These findings demonstrate that tobacco waste is a valuable source of bioactive compounds and MAE can be a promising alternative technique to obtain extracts rich in targeted bioactive compounds, especially nicotine.
Marija Banožić; Ines Banjari; Ivana Flanjak; Mate Paštar; Jelena Vladić; Stela Jokić. Optimization of MAE for the Separation of Nicotine and Phenolics from Tobacco Waste by Using the Response Surface Methodology Approach. Molecules 2021, 26, 4363 .
AMA StyleMarija Banožić, Ines Banjari, Ivana Flanjak, Mate Paštar, Jelena Vladić, Stela Jokić. Optimization of MAE for the Separation of Nicotine and Phenolics from Tobacco Waste by Using the Response Surface Methodology Approach. Molecules. 2021; 26 (14):4363.
Chicago/Turabian StyleMarija Banožić; Ines Banjari; Ivana Flanjak; Mate Paštar; Jelena Vladić; Stela Jokić. 2021. "Optimization of MAE for the Separation of Nicotine and Phenolics from Tobacco Waste by Using the Response Surface Methodology Approach." Molecules 26, no. 14: 4363.
The triboelectric nanogenerator (TENG) is a widely used energy-harvesting unit for self-power applications. The electrical output performance of the TENG could be significantly improved by ion implantation and explore new triboelectric materials beyond the conventional triboelectric series. In this present work, the phase pure hydrothermally synthesized ZnO particles, argon ion-implanted Kapton acted as a positive triboelectric layer while pure Kapton behaved as a negative triboelectric layer. The atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) were performed to investigate the surface roughness and surface potential of the triboelectric materials utilized in vertical contact mode TENG (I-TENG, abbreviated further). The elemental mapping and X-ray photoelectron spectroscopy (XPS) results suggested successful Argon-ion implantation upon Kapton. Finally, the I-TENG device was subjected to various forces to systematically depict its electrical output responses and power up a wrist-watch and calculator.
Manisha Sahu; Silvija Šafranko; Sugato Hajra; Aneeta Manjari Padhan; Pavo Živković; Stela Jokić; Hoe Joon Kim. Development of triboelectric nanogenerator and mechanical energy harvesting using argon ion- implanted kapton, zinc oxide and kapton. Materials Letters 2021, 301, 130290 .
AMA StyleManisha Sahu, Silvija Šafranko, Sugato Hajra, Aneeta Manjari Padhan, Pavo Živković, Stela Jokić, Hoe Joon Kim. Development of triboelectric nanogenerator and mechanical energy harvesting using argon ion- implanted kapton, zinc oxide and kapton. Materials Letters. 2021; 301 ():130290.
Chicago/Turabian StyleManisha Sahu; Silvija Šafranko; Sugato Hajra; Aneeta Manjari Padhan; Pavo Živković; Stela Jokić; Hoe Joon Kim. 2021. "Development of triboelectric nanogenerator and mechanical energy harvesting using argon ion- implanted kapton, zinc oxide and kapton." Materials Letters 301, no. : 130290.
With globalization, growing popularization and changes in dietary habits where more processed food is consumed, a generation of food wastes and by-products has increased. Under-exploitation of by-products and waste leads to finance losses and environmental pollution although most of the waste are a rich source of bioactive compounds. Using spray drying process, extracted bioactive compounds from food wastes and by-products can be transferred into powder form, and returned to the food chain. Spray drying technique showed to be the most convenient, energy efficient and time-saving process, where a liquid is directly transformed into stable powder, which can be stored for a prolonged time or easily incorporated into other products. This review article focuses on the application of processing parameters of spray drying with specific emphasis on the production of dry extracts of food waste and their influence on powder properties. Research gaps and challenges along with possible applications are also discussed.
Marija Banožić; Jelena Vladiä‡; Ines Banjari; Darko Velić; Krunoslav Aladić; Stela Jokić. Spray Drying as a Method of Choice for Obtaining High Quality Products from Food Wastes– A Review. Food Reviews International 2021, 1 -33.
AMA StyleMarija Banožić, Jelena Vladiä‡, Ines Banjari, Darko Velić, Krunoslav Aladić, Stela Jokić. Spray Drying as a Method of Choice for Obtaining High Quality Products from Food Wastes– A Review. Food Reviews International. 2021; ():1-33.
Chicago/Turabian StyleMarija Banožić; Jelena Vladiä‡; Ines Banjari; Darko Velić; Krunoslav Aladić; Stela Jokić. 2021. "Spray Drying as a Method of Choice for Obtaining High Quality Products from Food Wastes– A Review." Food Reviews International , no. : 1-33.
The fluorescent carbon quantum dots (CQDs) represent an emerging subset of carbonaceous nanomaterials, recently becoming a powerful tool for biosensing, bioimaging, and drug and gene delivery. In general, carbon dots are defined as zero-dimensional (0D), spherical-like nanoparticles with <10 nm in size. Their unique chemical, optical, and electronic properties make CQDs versatile materials for a wide spectrum of applications, mainly for the sensing and biomedical purposes. Due to their good biocompatibility, water solubility, and relatively facile modification, these novel materials have attracted tremendous interest in recent years, which is especially important for nanotechnology and nanoscience expertise. The preparation of the biomass-derived CQDs has attracted growing interest recently due to their low-cost, renewable, and green biomass resources, presenting also the variability of possible modification for the enhancement of CQDs’ properties. This review is primarily focused on the recent developments in carbon dots and their application in the sensing of different chemical species within the last five years. Furthermore, special emphasis has been made regarding the green approaches for obtaining CQDs and nanomaterial characterization toward better understanding the mechanisms of photoluminescent behavior and sensing performance. In addition, some of the challenges and future outlooks in CQDs research have been briefly outlined.
Silvija Šafranko; Dominik Goman; Anamarija Stanković; Martina Medvidović-Kosanović; Tihomir Moslavac; Igor Jerković; Stela Jokić. An Overview of the Recent Developments in Carbon Quantum Dots—Promising Nanomaterials for Metal Ion Detection and (Bio)Molecule Sensing. Chemosensors 2021, 9, 138 .
AMA StyleSilvija Šafranko, Dominik Goman, Anamarija Stanković, Martina Medvidović-Kosanović, Tihomir Moslavac, Igor Jerković, Stela Jokić. An Overview of the Recent Developments in Carbon Quantum Dots—Promising Nanomaterials for Metal Ion Detection and (Bio)Molecule Sensing. Chemosensors. 2021; 9 (6):138.
Chicago/Turabian StyleSilvija Šafranko; Dominik Goman; Anamarija Stanković; Martina Medvidović-Kosanović; Tihomir Moslavac; Igor Jerković; Stela Jokić. 2021. "An Overview of the Recent Developments in Carbon Quantum Dots—Promising Nanomaterials for Metal Ion Detection and (Bio)Molecule Sensing." Chemosensors 9, no. 6: 138.
In this study, an efficient utilization and valorization of mandarin peel (Citrus unshiu Marc. var. Kuno) was investigated using innovative and green extraction techniques. The first step of this study included the extraction and analysis of the volatile compounds by performing a supercritical CO2 (SC-CO2) extraction under different operating pressure conditions (100 and 300 bar). The analysis of volatile compounds of the obtained extracts was conducted by gas chromatography-mass spectrometry (GC-MS), and limonene was found to be the dominant volatile component (13.16% at 100 bar; 30.65% at 300 bar). After SC-CO2 treatment, the exhausted citrus peel waste enriched with bioactive compounds was subjected to subcritical water extraction (SWE) in a wide temperature range (130–220 °C) using different solvent-solid ratio (10–30 mL/g) in time periods from 5 to 15 min, in order to obtain bioflavonoids. Identification and quantification of present bioflavonoids was conducted by high-performance liquid chromatography with a with a diode array detector (HPLC), and hesperidin (0.16–15.07 mg/g) was determined as the most abundant flavanon in mandarin peel with other polyphenolic compounds that were possible by-products of thermal degradation. At higher temperatures, the presence of 5-hydroxymethylfurfural (5-HMF) and chlorogenic acid were detected. Antiradical activity and total phenolic content in the extracts were determined using spectrophotometric methods, while the process optimization was performed by response surface methodology (RSM).
Silvija Šafranko; Ina Ćorković; Igor Jerković; Martina Jakovljević; Krunoslav Aladić; Drago Šubarić; Stela Jokić. Green Extraction Techniques for Obtaining Bioactive Compounds from Mandarin Peel (Citrus unshiu var. Kuno): Phytochemical Analysis and Process Optimization. Foods 2021, 10, 1043 .
AMA StyleSilvija Šafranko, Ina Ćorković, Igor Jerković, Martina Jakovljević, Krunoslav Aladić, Drago Šubarić, Stela Jokić. Green Extraction Techniques for Obtaining Bioactive Compounds from Mandarin Peel (Citrus unshiu var. Kuno): Phytochemical Analysis and Process Optimization. Foods. 2021; 10 (5):1043.
Chicago/Turabian StyleSilvija Šafranko; Ina Ćorković; Igor Jerković; Martina Jakovljević; Krunoslav Aladić; Drago Šubarić; Stela Jokić. 2021. "Green Extraction Techniques for Obtaining Bioactive Compounds from Mandarin Peel (Citrus unshiu var. Kuno): Phytochemical Analysis and Process Optimization." Foods 10, no. 5: 1043.
The endemic brown macroalga Fucus virsoides J. Agardh from the Adriatic Sea was in the focus of the present research. The volatiles of fresh (FrFv) and air-dried (DrFv) samples of F. virsoides obtained by headspace solid-phase microextraction (HS-SPME) and hydrodistillation (HD) were analyzed by gas chromatography equipped with flame ionization detector and mass spectrometry (GC-FID/MS). The major HS-FrFv compound was pentadecane (61.90–71.55%) followed by pentadec-1-ene (11.00–7.98%). In HS-DrFv, pentadec-1-ene was not present, and few lower aliphatic compounds appeared, as well as benzaldehyde and benzyl alcohol. In HD-FrFv, particularly abundant were alkenes (such as pentadec-1-ene (19.32%), or (E)-pentadec-7-ene (8.35%)). In HD-DrFv, more oxidation products were present (e.g., carbonyl compounds such as tridecanal (18.51%)). The fatty acids profile of freeze-dried sample (FdFv) after conversion to methyl esters was determined by GC-FID, and oleic acid was dominant (42.28%), followed by arachidonic acid (15.00%). High-performance liquid chromatography-high-resolution mass spectrometry with electrospray ionization (HPLC-ESI-HRMS) was used for the screening of less polar fractions (F3 and F4) of F. virsoides. Mono- and diglycerides of stearic, palmitic, oleic, and arachidonic acids were found. Terpenoids and steroids comprised the compounds C20H30(32)O2 and C29H48O(2). Among carotenoids, fucoxanthin was identified. Chlorophyll derivatives were also found (C55H74(72)N4O(5-7)), dominated by pheophytin a. The antioxidant activity of the fractions was investigated by in vitro assays (oxygen radical absorbance capacity (ORAC), reduction of radical cation (ABTS•+), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, and ferric reducing antioxidant power (FRAP)) and by in vivo zebrafish model (along with fish embryotoxicity). In vitro experiments proved good radical scavenging abilities of F3 and F4 fractions, which were additionally supported by the protective effect against hydrogen peroxide-induced oxidative stress in zebrafish embryos.
Igor Jerković; Ana-Marija Cikoš; Sanja Babić; Lara Čižmek; Krunoslav Bojanić; Krunoslav Aladić; Nikolay Ul’Yanovskii; Dmitry Kosyakov; Albert Lebedev; Rozelindra Čož-Rakovac; Polonca Trebše; Stela Jokić. Bioprospecting of Less-Polar Constituents from Endemic Brown Macroalga Fucus virsoides J. Agardh from the Adriatic Sea and Targeted Antioxidant Effects In Vitro and In Vivo (Zebrafish Model). Marine Drugs 2021, 19, 235 .
AMA StyleIgor Jerković, Ana-Marija Cikoš, Sanja Babić, Lara Čižmek, Krunoslav Bojanić, Krunoslav Aladić, Nikolay Ul’Yanovskii, Dmitry Kosyakov, Albert Lebedev, Rozelindra Čož-Rakovac, Polonca Trebše, Stela Jokić. Bioprospecting of Less-Polar Constituents from Endemic Brown Macroalga Fucus virsoides J. Agardh from the Adriatic Sea and Targeted Antioxidant Effects In Vitro and In Vivo (Zebrafish Model). Marine Drugs. 2021; 19 (5):235.
Chicago/Turabian StyleIgor Jerković; Ana-Marija Cikoš; Sanja Babić; Lara Čižmek; Krunoslav Bojanić; Krunoslav Aladić; Nikolay Ul’Yanovskii; Dmitry Kosyakov; Albert Lebedev; Rozelindra Čož-Rakovac; Polonca Trebše; Stela Jokić. 2021. "Bioprospecting of Less-Polar Constituents from Endemic Brown Macroalga Fucus virsoides J. Agardh from the Adriatic Sea and Targeted Antioxidant Effects In Vitro and In Vivo (Zebrafish Model)." Marine Drugs 19, no. 5: 235.
Three fractions of tobacco waste (scrap, dust and midrib) were subjected to a high voltage electric discharge (HVED) assisted extraction procedure under different experimental conditions: solvent:solid ratio (300, 500, 700 mL/g), frequency (40, 70, 100 Hz) and treatment time (15, 30, 45 min), in order to study the influence of these conditions on the content of chlorogenic acid. The content of chlorogenic acid ranged from 1.54 to 3.66 mg/100 g for scrap, from 1.90 to 2.97 mg/100 g for dust, and from 2.30 to 3.38 mg/100 g for midrib extract, showing a strong dependence on the applied process parameters. The temperature change and the change in pH and electrical conductivity of the extracts after high voltage discharge treatment were also observed. The studied process parameters showed a statistically significant effect on the chemical and physical properties of the extracts from tobacco waste as well as on the content of chlorogenic acid, indicating the potential of HVED assisted processes in the separation of chlorogenic acid from tobacco industry waste. Multiple regression analysis was used to fit the results for the chlorogenic acid to a second order polynomial equation and the optimum conditions were determined.
Marija Banožić; Antun Jozinović; Jovana Grgić; Borislav Miličević; Stela Jokić. High Voltage Electric Discharge for Recovery of Chlorogenic Acid from Tobacco Waste. Sustainability 2021, 13, 4481 .
AMA StyleMarija Banožić, Antun Jozinović, Jovana Grgić, Borislav Miličević, Stela Jokić. High Voltage Electric Discharge for Recovery of Chlorogenic Acid from Tobacco Waste. Sustainability. 2021; 13 (8):4481.
Chicago/Turabian StyleMarija Banožić; Antun Jozinović; Jovana Grgić; Borislav Miličević; Stela Jokić. 2021. "High Voltage Electric Discharge for Recovery of Chlorogenic Acid from Tobacco Waste." Sustainability 13, no. 8: 4481.
Due to the lack of phytochemical composition data, the major goals of the present study on Amphiroa rigida J.V. Lamouroux were to: (a) investigate and compare volatilome profiles of fresh and air-dried samples obtained by headspace solid-phase microextraction (HS-SPME) and hydrodistillation (HD) followed by gas chromatography and mass spectrometry (GC/MS) analysis; (b) determine fatty acids profile by gas chromatography with flame ionization detector (GC-FID); (c) obtain the pigment profiles of semipurified extracts by high performance liquid chromatography (HPLC) and (d) evaluate the antioxidant and antimicrobial activities of its less polar fractions. The comparison of headspace of fresh (FrAr) and air-dried (DrAr) samples revealed many similarities regarding the presence and abundance of the major (heptadecane and pentadecane) and minor compounds. The hydrodistillate (HD) of DrAr profile was quite different in comparison to HD-FrAr. The predominant compound in HD-FrAr was (E)-phytol. In HD-DrAr, its percentage was approximately one-half reduced, but the abundance of its degradation product phytone and of unsaturated and oxygenated compounds increased indicating more intense fatty acid decomposition and oxidation during drying. The fatty acid determination revealed that the most dominant was palmitic acid (42.86%) followed by eicosapentaenoic acid (19.14%) and stearic acid (11.65%). Among the pigments, A. rigida contained fucoxanthin (0.63 mg g−1 of dry fraction), lutein (5.83 mg g−1), β-carotene (6.18 mg g−1) and chlorophyll a (13.65 mg g−1). The analyzed less polar fractions of A. rigida exhibited antioxidant scavenging activity with diammonium salt of 2,2′-azino-bis (3-ethylbenzthiazolin-6-yl) sulfonic acid (ABTS) assay up to 3.87 mg g−1 trolox equivalents (TE), and with the oxygen radical absorbance capacity (ORAC) assay up to 825.63 μmol g−1 TE (with carotenoids as the major contributors).
Ana-Marija Cikoš; Ivana Flanjak; Krunoslav Bojanić; Sanja Babić; Lara Čižmek; Rozelindra Čož-Rakovac; Stela Jokić; Igor Jerković. Bioprospecting of Coralline Red Alga Amphiroa rigida J.V. Lamouroux: Volatiles, Fatty Acids and Pigments. Molecules 2021, 26, 520 .
AMA StyleAna-Marija Cikoš, Ivana Flanjak, Krunoslav Bojanić, Sanja Babić, Lara Čižmek, Rozelindra Čož-Rakovac, Stela Jokić, Igor Jerković. Bioprospecting of Coralline Red Alga Amphiroa rigida J.V. Lamouroux: Volatiles, Fatty Acids and Pigments. Molecules. 2021; 26 (3):520.
Chicago/Turabian StyleAna-Marija Cikoš; Ivana Flanjak; Krunoslav Bojanić; Sanja Babić; Lara Čižmek; Rozelindra Čož-Rakovac; Stela Jokić; Igor Jerković. 2021. "Bioprospecting of Coralline Red Alga Amphiroa rigida J.V. Lamouroux: Volatiles, Fatty Acids and Pigments." Molecules 26, no. 3: 520.
Salvia officinalis L. is a good source of antioxidant compounds such as phenolic diterpenes carnosic acid and carnosol. From 17 deep eutectic solvents (DESs) used, choline chloride: lactic acid (1:2 molar ratio) was found to be the most suitable for the extraction of targeted compounds. The influence of H2O content, extraction time, and temperature (for stirring and heating and for ultrasound-assisted extraction (UAE)), H2O content, extraction time, and vibration speed for mechanochemical extraction on the content of targeted compounds were investigated. Carnosic acid content obtained by the extraction assisted by stirring and heating was from 2.55 ± 0.04 to 14.43 ± 0.28 µg mg−1, for UAE it was from 1.62 ± 0.29 to 14.00 ± 0.02 µg mg−1, and for mechanochemical extraction the yield was from 1.80 ± 0.02 to 8.26 ± 0.45 µg mg−1. Determined carnosol content was in the range 0.81 ± 0.01 to 4.83 ± 0.09 µg mg−1 for the extraction with stirring and for UAE it was from 0.56 ± 0.02 to 4.18 ± 0.05 µg mg−1, and for mechanochemical extraction the yield was from 0.57 ± 0.11 to 2.01 ± 0.16 µg mg−1. Optimal extraction conditions determined by response surface methodology (RSM) were in accordance with experimentally demonstrated values. In comparison with previously published or own results using conventional solvents or supercritical CO2, used DES provided more efficient extraction of both targeted compounds.
Martina JakovljeviĆ; Stela Jokić; Maja Molnar; Igor Jerković. Application of Deep Eutectic Solvents for the Extraction of Carnosic Acid and Carnosol from Sage (Salvia officinalis L.) with Response Surface Methodology Optimization. Plants 2021, 10, 80 .
AMA StyleMartina JakovljeviĆ, Stela Jokić, Maja Molnar, Igor Jerković. Application of Deep Eutectic Solvents for the Extraction of Carnosic Acid and Carnosol from Sage (Salvia officinalis L.) with Response Surface Methodology Optimization. Plants. 2021; 10 (1):80.
Chicago/Turabian StyleMartina JakovljeviĆ; Stela Jokić; Maja Molnar; Igor Jerković. 2021. "Application of Deep Eutectic Solvents for the Extraction of Carnosic Acid and Carnosol from Sage (Salvia officinalis L.) with Response Surface Methodology Optimization." Plants 10, no. 1: 80.
The stability of chocolate is mainly influenced by cocoa butter re-crystallization during storage, shown through fat bloom (appearance of white layers of cocoa butter on chocolate surface). The aim of this study was to examine the influence of cocoa shell addition on stability of dark and milk chocolate over a one-year storage period. Dark and milk chocolates were produced in a ball mill. Four chocolates were produced, two with the addition of cocoa shell (5% in milk and 15% in dark) and two control chocolates without the addition. The effect of cocoa shell on chocolate stability was examined by determining total color change and whiteness index (calculated from parameters L*, a* and b* determined using chromameter), total polyphenol content (TPC) (using the Folin–Ciocalteau method) and thermo-physical properties (using differential scanning calorimetry). Total color change and whiteness index were lower in chocolates with cocoa shell than in control samples. Over a period of one year, milk and dark chocolate with cocoa shell had lower enthalpy. Total polyphenol content in all chocolates did not change significantly through the storage period, although chocolates with cocoa shell had lower TPC because part of the cocoa liquor was replaced with cocoa shell.
Veronika Barišić; Ante Lončarić; Ivana Flanjak; Antun Jozinović; Stela Jokić; Drago Šubarić; Jurislav Babić; Borislav Miličević; Đurđica Ačkar. Stability of Chocolates Enriched with Cocoa Shell during Storage. Proceedings 2020, 70, 3 .
AMA StyleVeronika Barišić, Ante Lončarić, Ivana Flanjak, Antun Jozinović, Stela Jokić, Drago Šubarić, Jurislav Babić, Borislav Miličević, Đurđica Ačkar. Stability of Chocolates Enriched with Cocoa Shell during Storage. Proceedings. 2020; 70 (1):3.
Chicago/Turabian StyleVeronika Barišić; Ante Lončarić; Ivana Flanjak; Antun Jozinović; Stela Jokić; Drago Šubarić; Jurislav Babić; Borislav Miličević; Đurđica Ačkar. 2020. "Stability of Chocolates Enriched with Cocoa Shell during Storage." Proceedings 70, no. 1: 3.
Satureja montana herbal species belongs to aromatic medicinal plants with a significant place in traditional medicine. However, products produced with conventional procedures do not meet the requirements of the modern market which include environmentally-safe processes that provide quality, safe, and standardized products. In this study, the antiproliferative activity of S. montana extracts obtained by supercritical carbon dioxide and solid–liquid extraction followed by spray drying was investigated using the in vivo model of Ehrlich ascites carcinoma (EAC) in mice. The impact of two concentrations of extracts on the growth of tumor and the redox status of malignant cells was monitored. It was determined that the extracts induced oxidative stress in the malignant cells which was confirmed by the changes in activity of biochemical indicators of oxidative stress. The posttreatment was not an efficient approach, while the extracts applied as pretreatment and treatment resulted in an increase in the xanthine oxidase (XOD) activity, a decrease in catalase (CAT) activity, and an increase in the intensity of lipid peroxidation (LPx). Furthermore, a decrease in the values of reduced glutathione (GSH) and an increase in glutathione reductase (GR) and glutathione peroxidase (GSHPx) in EAC cells were recorded.
Jelena Vladić; Tatjana Ćebović; Senka Vidović; Stela Jokić. Evaluation of Anticancer Activity of Satureja montana Supercritical and Spray-Dried Extracts on Ehrlich’s Ascites Carcinoma Bearing Mice. Plants 2020, 9, 1532 .
AMA StyleJelena Vladić, Tatjana Ćebović, Senka Vidović, Stela Jokić. Evaluation of Anticancer Activity of Satureja montana Supercritical and Spray-Dried Extracts on Ehrlich’s Ascites Carcinoma Bearing Mice. Plants. 2020; 9 (11):1532.
Chicago/Turabian StyleJelena Vladić; Tatjana Ćebović; Senka Vidović; Stela Jokić. 2020. "Evaluation of Anticancer Activity of Satureja montana Supercritical and Spray-Dried Extracts on Ehrlich’s Ascites Carcinoma Bearing Mice." Plants 9, no. 11: 1532.
In this study, green extraction methods—high voltage electrical discharges (HVED), pulsed electric field (PEF), and ultrasound-assisted extraction (UAE)—were compared in terms of extraction yield of total and individual polyphenolic compounds, as well as the antioxidant capacity of blueberry pomace extracts. All extractions were performed with methanol- and ethanol-based solvents. The highest total polyphenols content (TPC) (10.52 mg of gallic acid equivalent (GAE) per g of dry weight (dw)) and antioxidant activity (AA) (0.83 mmol TE/g dw) were obtained by PEF-assisted extraction in the ethanol-based solvent after 100 pulses and 20 kV/cm, which corresponds to an energy input of 41.03 kJ/kg. A total of eighteen individual polyphenols were identified in all investigated blueberry pomace extracts by high-performance liquid chromatography with the diode-array detector (HPLC-DAD) and liquid chromatography electrospray ionization tandem mass spectrometric (LC-(HESI)-MS/MS). The highest anthocyanin (1757.32 µg/g of dw) and flavanol (297.86 µg/g of dw) yields were obtained in the methanol-based solvent, while the highest phenolic acid (625.47 µg/g of dw) and flavonol (157.54 µg/g of dw) yields were obtained in the ethanol-based solvent by PEF-assisted extraction at the energy input of 41.03 kJ/kg. These results indicated that PEF is a promising green extraction method which can improve the blueberry pomace’s polyphenol extraction yield.
Ante Lončarić; Maria Celeiro; Antun Jozinović; Josip Jelinić; Tihomir Kovač; Stela Jokić; Jurislav Babić; Tihomir Moslavac; Sandra Zavadlav; Marta Lores. Green Extraction Methods for Extraction of Polyphenolic Compounds from Blueberry Pomace. Foods 2020, 9, 1521 .
AMA StyleAnte Lončarić, Maria Celeiro, Antun Jozinović, Josip Jelinić, Tihomir Kovač, Stela Jokić, Jurislav Babić, Tihomir Moslavac, Sandra Zavadlav, Marta Lores. Green Extraction Methods for Extraction of Polyphenolic Compounds from Blueberry Pomace. Foods. 2020; 9 (11):1521.
Chicago/Turabian StyleAnte Lončarić; Maria Celeiro; Antun Jozinović; Josip Jelinić; Tihomir Kovač; Stela Jokić; Jurislav Babić; Tihomir Moslavac; Sandra Zavadlav; Marta Lores. 2020. "Green Extraction Methods for Extraction of Polyphenolic Compounds from Blueberry Pomace." Foods 9, no. 11: 1521.
The aim of this study was to evaluate Croatian pepper seed varieties (Podravka and Slavonka) as a new source of added value ingredients. Pepper seed is mostly considered a by-product. For the first time, the pepper (Capsicum annuum L.) seeds of the Slavonka and Podravka varieties were examined as the source material for oil production by the two methods of extraction: cold pressing (CP) and supercritical CO2 extraction (SC-CO2). Further, fatty acid profile, tocopherols, and sensory analysis of the oils were examined, as well as the chemical characteristics and antioxidant potential of seed flour. The antioxidant potential of pepper seed flour was different between varieties (Podravka 107 antioxidant unit (AU); Slavonka 70 antioxidant unit (AU)). The Podravka variety pepper seed oil has shown higher γ-tocopherol content (CP 80.1 mg/100 g; SC-CO2 extraction 65.3 mg/100 g) than the Slavonka variety (CP 65.3 mg/100 g; SC-CO2 extraction 16.0 mg/100 g). According to the obtained results, cold pressing (CP) would be a more favourable method for pepper seed oil extraction, taking into account sensory evaluation and nutritional quality. The pepper seed oil has potential for culinary application with a nutritional quality comparable to vegetable oils of a higher price class.
Tanja Cvetković; Jasmina Ranilović; Davorka Gajari; Helena Tomić-Obrdalj; Drago Šubarić; Tihomir Moslavac; Ana-Marija Cikoš; Stela Jokić. Podravka and Slavonka Varieties of Pepper Seeds (Capsicum annuum L.) as a New Source of Highly Nutritional Edible Oil. Foods 2020, 9, 1262 .
AMA StyleTanja Cvetković, Jasmina Ranilović, Davorka Gajari, Helena Tomić-Obrdalj, Drago Šubarić, Tihomir Moslavac, Ana-Marija Cikoš, Stela Jokić. Podravka and Slavonka Varieties of Pepper Seeds (Capsicum annuum L.) as a New Source of Highly Nutritional Edible Oil. Foods. 2020; 9 (9):1262.
Chicago/Turabian StyleTanja Cvetković; Jasmina Ranilović; Davorka Gajari; Helena Tomić-Obrdalj; Drago Šubarić; Tihomir Moslavac; Ana-Marija Cikoš; Stela Jokić. 2020. "Podravka and Slavonka Varieties of Pepper Seeds (Capsicum annuum L.) as a New Source of Highly Nutritional Edible Oil." Foods 9, no. 9: 1262.
The nutritional and bioactive content of banana and red beetroot peels was investigated. The basic macrocomponent composition was determined using standard AOAC (Association of Official Analytical Chemists) methods, while the recovery efficiency of bioactive compounds was investigated using conventional and innovative extraction techniques (subcritical water extraction, ultrasound- and microwave-assisted extraction). Extracts were analyzed for biological effects in vitro on human hepatic, tongue and colon cancer cell lines. A macrocomponent analysis revealed a notable amount of dietary fiber in banana and beetroot peels (39.0 and 33.6% dmb) and a relatively high content of protein in beetroot peel (18.3% dmb). Regarding the micronutrients-minerals, banana and beetroot peels were shown to be a very good source of potassium (75.06 and 41.86 mg g−1 dmb). Both extracts of banana and beetroot peels obtained by conventional extraction - decoction (100 °C, 20 min) exhibited the highest total phenolic content and antioxidant capacity. Additionally, in banana peel, these extracts were the richest in dopamine content (12.63 mg g−1 dmb). Extraction by infusion (80 °C, 30 min) yielded a beetroot peel extract with the highest total betacyanin content (9.80 mg g−1 dmb). Biological effects in vitro were dose- and time-dependent, as well as influenced by the presence of polysaccharides.
Danijela Šeremet; Ksenija Durgo; Stela Jokić; Ana Huđek; Aleksandra Vojvodić Cebin; Ana Mandura; Jasna Jurasović; Draženka Komes. Valorization of Banana and Red Beetroot Peels: Determination of Basic Macrocomponent Composition, Application of Novel Extraction Methodology and Assessment of Biological Activity In Vitro. Sustainability 2020, 12, 4539 .
AMA StyleDanijela Šeremet, Ksenija Durgo, Stela Jokić, Ana Huđek, Aleksandra Vojvodić Cebin, Ana Mandura, Jasna Jurasović, Draženka Komes. Valorization of Banana and Red Beetroot Peels: Determination of Basic Macrocomponent Composition, Application of Novel Extraction Methodology and Assessment of Biological Activity In Vitro. Sustainability. 2020; 12 (11):4539.
Chicago/Turabian StyleDanijela Šeremet; Ksenija Durgo; Stela Jokić; Ana Huđek; Aleksandra Vojvodić Cebin; Ana Mandura; Jasna Jurasović; Draženka Komes. 2020. "Valorization of Banana and Red Beetroot Peels: Determination of Basic Macrocomponent Composition, Application of Novel Extraction Methodology and Assessment of Biological Activity In Vitro." Sustainability 12, no. 11: 4539.
In the present study, valorization of yarrow (Achillea millefolium) by-product from the filter tea industry was investigated through the application of subcritical water for the extraction of bioactive compounds. The influence of different process parameters (temperature 120–200 °C, extraction time 10–30 min, and HCl concentration in extraction solvent 0–1.5%) on extract quality in terms of content of bioactive compounds and antioxidant activity was investigated. Optimal conditions of the extraction process (temperature 198 °C, extraction time 16.5 min, and without acidifer) were determined and, when applied, the most efficient exploitation of by-products is achieved, that is, attainment of extracts rich in total phenols and flavonoids and high antioxidant activity. In addition, by applying the high performance liquid chromatographic analysis, the content of chlorogenic acid was determined as well as the hydroxymethylfurfural content in obtained extracts. The results demonstrated that subcritical water can be successfully used for utilization of yarrow by-products for obtaining extracts rich in antioxidants.
Jelena Vladić; Martina JakovljeviĆ; Maja Molnar; Senka Vidović; Milan Tomić; Zorica Drinić; Stela Jokić. Valorization of Yarrow (Achillea millefolium L.) By-Product through Application of Subcritical Water Extraction. Molecules 2020, 25, 1878 .
AMA StyleJelena Vladić, Martina JakovljeviĆ, Maja Molnar, Senka Vidović, Milan Tomić, Zorica Drinić, Stela Jokić. Valorization of Yarrow (Achillea millefolium L.) By-Product through Application of Subcritical Water Extraction. Molecules. 2020; 25 (8):1878.
Chicago/Turabian StyleJelena Vladić; Martina JakovljeviĆ; Maja Molnar; Senka Vidović; Milan Tomić; Zorica Drinić; Stela Jokić. 2020. "Valorization of Yarrow (Achillea millefolium L.) By-Product through Application of Subcritical Water Extraction." Molecules 25, no. 8: 1878.
Carbohydrates are important compounds in natural products where they primarily serve as a source of energy, but they have important secondary roles as precursors of aroma or bioactive compounds. They are present in fresh and dried (cured) tobacco leaves as well. The sugar content of tobacco depends on the tobacco variety, harvesting, and primarily on the curing conditions (temperature, time and moisture). If the process of curing employs high temperatures (flue-curing and sun-curing), final sugar content is high. In contrast, when air curing has a lower temperature, at the end of the process, sugar level is low. Beside simple sugars, other carbohydrates reported in tobacco are oligosaccharides, cellulose, starch, and pectin. Degradation of polysaccharides results in a higher yield of simple sugars, but at the same time reduces sugars oxidization and transfer into carbon dioxide and water. Loss of sugar producers will compensate with added sugars, to cover undesirable aroma properties and achieve a better, pleasant taste during smoking. However, tobacco carbohydrates can be precursors for many harmful compounds, including formaldehyde and 5-hydroxymethylfurfural. Keeping in mind that added sugars in tobacco production are unavoidable, it is important to understand all changes in carbohydrates from harvesting to consuming in order to achieve better product properties and avoid the formation of harmful compounds. This review summarizes current knowledge about tobacco carbohydrates, including changes during processing with special focus on carbohydrates as precursors of harmful compounds during smoking.
Marija Banožić; Stela Jokić; Đurđica Ačkar; Marijana Blažić; Drago Šubarić. Carbohydrates—Key Players in Tobacco Aroma Formation and Quality Determination. Molecules 2020, 25, 1734 .
AMA StyleMarija Banožić, Stela Jokić, Đurđica Ačkar, Marijana Blažić, Drago Šubarić. Carbohydrates—Key Players in Tobacco Aroma Formation and Quality Determination. Molecules. 2020; 25 (7):1734.
Chicago/Turabian StyleMarija Banožić; Stela Jokić; Đurđica Ačkar; Marijana Blažić; Drago Šubarić. 2020. "Carbohydrates—Key Players in Tobacco Aroma Formation and Quality Determination." Molecules 25, no. 7: 1734.
The aim of this study was to establish an efficient, sustainable technological procedure for valorization of food by-product, that is, cocoa bean shells (CBSs). The properties and stability of CBS extracts obtained by spray drying process with maltodextrin (MD) and whey protein (WP) as carrier agents were evaluated. For this purpose, phytochemicals of CBSs were extracted by subcritical water extraction. Physico-chemical properties, total phenolic (TP) and total flavonoid (TF) contents of the encapsulated extracts were determined in order to verify the efficiency of spray drying. Additional analyses for phytochemical characterization of the obtained powders were also performed. The efficiency of microencapsulation process was characterized by product recoveries higher than 58%. Both coating materials significantly influenced the encapsulation of phytochemicals in terms of rehydration, water solubility index and water absorption index, with WP being at an advantage. The best results for TP and TF contents were achieved when CBSs were encapsulated using WP (37.68 mg GAE/g and 7.66 mg CE/g, respectively). Microencapsulation using WP yielded higher content of gallic acid, caffeine, and theobromine than those with MD. According to the results, the formulation using 50% WP provided a better preservation of polyphenols compared to 50% MD. Therefore, spray drying with WP can be used as a method of choice for obtaining high quality CBS powders.
Stela Jokić; Nataša Nastić; Senka Vidović; Ivana Flanjak; Krunoslav Aladić; Jelena Vladić. An Approach to Value Cocoa Bean By-Product Based on Subcritical Water Extraction and Spray Drying Using Different Carriers. Sustainability 2020, 12, 2174 .
AMA StyleStela Jokić, Nataša Nastić, Senka Vidović, Ivana Flanjak, Krunoslav Aladić, Jelena Vladić. An Approach to Value Cocoa Bean By-Product Based on Subcritical Water Extraction and Spray Drying Using Different Carriers. Sustainability. 2020; 12 (6):2174.
Chicago/Turabian StyleStela Jokić; Nataša Nastić; Senka Vidović; Ivana Flanjak; Krunoslav Aladić; Jelena Vladić. 2020. "An Approach to Value Cocoa Bean By-Product Based on Subcritical Water Extraction and Spray Drying Using Different Carriers." Sustainability 12, no. 6: 2174.
Mast jazavca ima ljekovita svojstva koja proizlaze iz načina ishrane jazavca. Životinjska mast podliježe oksidacijskom kvarenju tijekom proizvodnje, skladištenja i toplinske obrade. U ovom radu istraživan je utjecaj dodatka prirodnih antioksidanasa (ekstrakt ružmarina, ekstrakt kadulje, alfa tokoferol, mješavina tokoferola) i sintetskog antioksidansa propil galata na promjenu oksidacijske stabilnosti masti jazavca. Oksidacijska stabilnost masti jazavca, sa i bez dodanog antioksidansa, ispitivana je primjenom testa održivosti na 98 °C. Rezultati testa prikazani su kao vrijednost peroksidnog broja nakon određenog vremena držanja uzorka pri temperaturi 98 °C. Rezultati istraživanja pokazuju da dodani antioksidansi uspješno stabiliziraju mast jazavca. Od prirodnih antioksidanasa veću antioksidacijsku aktivnost u masti jazavca ima ekstrakt ružmarina tip Oxy’Less CS. Postigao je veću efikasnost zaštite od oksidacije, u odnosu na druge ispitivane antioksidanse. Sintetski antioksidans propil galat uspješno je povećao stabilnost masti jazavca, ali je manje efikasan od ekstrakta ružmarina i mješavine tokoferola.
Tihomir Moslavac; Anastazija Konjarević; Krunoslav Aladić; Drago Šubarić; Stela Jokić. Utjecaj dodatka antioksidanasa na oksidacijsku stabilnost masti jazavca. Meso 2020, 22, 46 -55.
AMA StyleTihomir Moslavac, Anastazija Konjarević, Krunoslav Aladić, Drago Šubarić, Stela Jokić. Utjecaj dodatka antioksidanasa na oksidacijsku stabilnost masti jazavca. Meso. 2020; 22 (1):46-55.
Chicago/Turabian StyleTihomir Moslavac; Anastazija Konjarević; Krunoslav Aladić; Drago Šubarić; Stela Jokić. 2020. "Utjecaj dodatka antioksidanasa na oksidacijsku stabilnost masti jazavca." Meso 22, no. 1: 46-55.