In order to discover new natural resources with biological properties, the chemical composition, the antioxidant and antimicrobial activities, and the potential use as food preservative of essential oils of Moroccan Achillea odorata subsp. pectinata (AO_pEO) and Ruta montana (RMEO) were studied. Gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of 21 and 25 compounds in AO_pEO and RMEO, respectively. The results showed that the major compounds of AO_pEO are camphor (45.01%), bornyl acetate (15.07%), borneol (11.33%), β-eudesmol (4.74%), camphene (3.58%), and 1.8-cineole (eucalyptol) (2.96%), whereas 2-undecanone (63.97%), camphor (3.82%) and cyclopropanecarboxylic acid (3.66%) were the main components of RMEO. The antioxidant activities were evaluated by diphenylpicrylhydraziyl radical (DPPH) and reducing power assays. The antimicrobial activities of essential oils were tested against bacterial strains and food contaminant yeast using agar disc diffusion and microdilution methods. A significant antimicrobial activity of AO_pEO was observed against Bacillus subtilis, Proteus mirabilis and Candida albicans, compared to RMEO. The efficacy of AO_pEO was also evaluated in model food systems (cabbage and barley) artificially inoculated during storage. The results found that the adding of a minimal inhibitory concentration (MIC) and 4× MIC were potent in decreasing the Proteus mirabilis growth in food model systems. Our findings suggested that AO_pEO may be potentially used as an alternative food preservative.

Climate and trade changes are reshaping the cartographic distribution of lethal pervasive pathogens. Among serious emerging challenges is Xylella fastidiosa (Xf), a xylem-limited phytopathogenic bacterium that produces losses and damages to numerous crops of high economic and agronomic importance. Lately, this grave quarantine pathogen has expended its distribution by arriving to several European countries and infecting both wild and cultivated plants, and no cure has been identified so far. Countries without current outbreaks like Morocco, need to monitor theirs crops frequently because detecting diseases in the early stages may reduce the huge losses caused by Xf. For that purpose, inspections were managed in different regions in Morocco from March 2020 to July 2021 to assess the presence of Xf in several growing areas of vulnerable economic crops (i.e., almond, citrus and olive). To extend the likelihood of detection, hosts have been inspected and sampled randomly over different environments including symptomatic and asymptomatic plants. Each sample was screened for the existence of Xf by using the DAS-ELISA commercial kit, while, further analyses were carried out for doubtful samples, by PCR. Results of both tests did not show any positive sample in the investigated areas. This finding is an update on the Xf situation in Morocco and confirms that this country is still a free territory from this bacterium, at least in the monitored regions.

Codling moth, Cydia pomonella L., is a major pest of pome fruits and walnuts worldwide. Screening the susceptibility of apple varieties to C. pomonella infestation is an important step to develop a sustainable management program against this pest. Therefore, the present study aimed to explore potential correlations between pest damage and several physical (firmness, weight, and diameter), chemical (pH, SSC, and TA), and biochemical (polyphenols) fruit properties collected from two sites with distinctively twelve and seven commercial apple varieties. The study was conducted in two distinct Moroccan regions to highlight whether the traits influencing the insect’s preference could be similar for different varieties under contrasted climates. In both regions, results revealed that the pest damage varied significantly across apple varieties as well as between observation dates. The late and medium maturing varieties had similar damage patterns and were heavily attacked compared to early maturing ones. The preferred varieties were Galaxy Gala, Obro Gala, Golden Smoothee, Black Staymen, and Idared, while the least preferred ones were Anna, Dorsett, and Stark Delicious. Some physical and chemical fruit properties seemed to be associated with the insect behavior, in particular, firmness and pH were positively correlated to pest damage. Moreover, the research suggested that infestations might be influenced by diameter, weight, and polyphenol content of fruits.

Olive leaf spot (OLS), also called olive scab and peacock eye, caused by Fusicladium oleagineum, is a major disease that causes significant damage to olive trees. However, we still lack information about how cultivar and environmental factors influence disease development. In this study, evaluation of the incidence and severity on twenty olive cultivars (Olea europaea L.), maintained in an ex situ collection in Morocco, was carried out monthly during the period from March to July 2021. Biochemical parameters were also evaluated for each cultivar including leaf chlorophyll, polyphenols and flavonoid contents. Results revealed that the OLS incidence was highly correlated with severity (r = 0.94) and found to be related to climatic conditions and cultivars. The studied cultivars were classified into four major groups, i.e., susceptible, moderately susceptible, moderately resistant and resistant. Finally, our investigations revealed a partial relationship between resistance to the OLS disease and phenolic and flavonoid leaf contents, supporting the assumption of the potential involvement of such components in cultivar resistance to the disease. Overall, our work highlights the importance of characterizing olive cultivar resistance to OLS in driving the choice of the best varieties for an effective control of the disease in specific warming regions such as Morocco.

Olive leaf spot (OLS), caused by Fusicladium oleagineum, is a significant disease affecting olive orchards, leading to reduced yields and compromising olive tree health. Early and accurate detection of this disease is critical for effective management. This study presents a comprehensive assessment of OLS disease progression in olive orchards by integrating agronomic measurements and multispectral imaging techniques. Key disease parameters—incidence, severity, diseased leaf area, and disease index—were systematically monitored from March to October, revealing peak values of 45% incidence in April and 35% severity in May. Multispectral drone imagery, using sensors for NIR, Red, Green, and Red Edge spectral bands, enabled the calculation of vegetation indices. Indices incorporating Red Edge and near-infrared bands, such as Red Edge and SR705-750, exhibited the strongest correlations with disease severity (correlation coefficients of 0.72 and 0.68, respectively). This combined approach highlights the potential of remote sensing for early disease detection and supports precision agriculture practices by facilitating targeted interventions and optimized orchard management. The findings underscore the effectiveness of integrating a traditional agronomic assessment with advanced spectral analysis to improve OLS disease surveillance and promote sustainable olive cultivation.

Soil salinity significantly impacts crop productivity. In response, plant growth-promoting rhizobacteria (PGPR) offer an innovative and eco-friendly solution to mitigate salinity stress. However, research on PGPR’s effects on crop physiology under varying salinity levels is still emerging. This study evaluates the impact of five bacterial strains, isolated from compost, on the growth of maize (Zea mays) and tomato (Solanum lycopersicum) plants under different levels of salt stress. This study involved treating maize and tomato seeds with five bacterial strains, and then planting them in a greenhouse under varying salt stress conditions (43 mM, 86 mM, 172 mM, 207 mM NaCl) using a Randomized Complete Block Design. Results showed that bacterial inoculation improved plant growth under saline conditions. S2015-1, S2026-2, and S2027-2 (Bacillus cereus, Acinetobacter calcoaceticus, Bacillus subtilis) were particularly effective in promoting plant growth under salt stress, especially at ionic concentrations of 43 mM and 86 mM, leading to a substantial increase in fresh and dry weight, with strain S2015-1 boosting chlorophyll by 29% at 86 mM in both crops. These results highlight the potential of PGPR to enhance crop resilience and productivity under salinity stress, promoting climate-smart agricultural practices.