Raspberries are important sources of bioactive compounds, whose synthesis is influenced by the fertilization system and the maturity stage. This study evaluated the effect of organic and conventional fertilization systems on raspberries at three maturity stages, pink, ripe, and overripe. Physicochemical characteristics, bioactive compounds (phenolic profile, vitamin C), antioxidant capacity (DPPH, FRAP, TEAC, and ORAC), phenolic-associated enzyme, phenylalanine ammonia lyase (PAL), and antioxidant enzymes (SOD, CAT, GPx, and APX) were evaluated. The physicochemical determination of the fruit did not reveal differences between fertilization systems. Regarding bioactive compounds, higher content of anthocyanins was found in organic raspberries at all maturity stages. Organic fertilization increased the content of ellagic acid and gallic acid at all stages of maturity. Higher content of caffeic, hydroxybenzoic, protocatechuic acid, and vitamin C was observed in organic raspberry at the overripe stage. Raspberries grown with organic fertilization exhibited higher values of antioxidant capacity by the DPPH, FRAP, and TEAC methods at all maturity stages. Raspberries under organic fertilization showed significantly greater activity of CAT, SOD, APX, GPX, and PAL. The present study suggests that organic fertilization induces oxidative stress causing an increase in antioxidant defense mechanisms, enhancing bioactive compound production, and improving antioxidant capacity in raspberries.

Edible films are thin preformed layers that provide food protection against adverse environmental conditions. Despite milk proteins being functional ingredients that can provide interesting features to films, there is scarce information evaluating their influence on film properties and stability. For this reason, this research work compared the mechanical (thickness, tensile strength, elongation at break), hydrodynamic (moisture content, water solubility, swelling ratio, water vapor transmission rate), color and antioxidant (DPPH) properties of edible films based on casein and whey protein isolate (two types, WPI₁ and WPI₂). Films with casein displayed the highest thickness (0.193 mm), elongation at break (49.67%), moisture content (40.21%) and antioxidant capacity (32.64% of DPPH inhibition), while obtaining the lowest water vapor transmission rate (15.28 g/m²·day). Significant differences were found in the color properties, mainly between films with casein and those made with WPI. Films containing WPI₁ and WPI₂ were statistically similar in thickness, tensile strength and color properties. The results showed that the properties of the edible films depended on the type of milk protein used. Thus, it is important to evaluate the features provided by different ingredients and formulations for obtaining edible films that properly preserve food.

Volatile compounds contribute to aroma and flavor, these being the main sensory attributes in food acceptance. This work addresses the physicochemical, volatile compounds, polyphenols, and flavonoids content and, antioxidant activity of apple-raspberry (90/10%) juice treated by thermal and ultraviolet radiation (UV-C) alone or in combination with moderate heat-treatment. Nineteen volatile compounds were identified which experienced changes depending on the treatment. Compounds such as α-ionone and β-ionone, that contribute to raspberries characteristic aroma, were present in a greater concentration in the UV-C treatment and lower in the thermal treatment. Likewise, 2-methyl butyl acetate, which give a fruity-sweet aroma typical of apples was present in a greater concentration in the UV-C treatment. Regarding polyphenol content, control and combined treatment presented the greater concentrations. However, after twenty days of storage, control and combined treatment presented the lower flavonoid concentration. Nevertheless, at this time, treatments showed no variations in antioxidant activity. Yeast and mold and total aerobic mesophilic and psychrophilic counts were reduced in the heat and combined treatments. In conclusion, UV-C and moderate heat might successfully be used to process a stable apple-raspberry juice while maintaining its quality and safety.

Pomegranate is known for high concentration of antioxidant phenolics contained in its seeds, and peel, and is considered “healthy” due to its nutritional content and biological actions. The aim of this study was to determine the nutritional quality, volatile and phenolic antioxidants in the juice, peel and seeds of pomegranate from two different regions of Mexico. The pomegranate variety Wonderful of the 2016 cycle, obtained from the Coyame and Cuauhtémoc regions of the state of Chihuahua, Mexico, were used to characterize the total phenolic content, antioxidant activity, nutritional quality and volatile compounds. Significant differences were found in evaluated parameters between the two regions. Pomegranates from Coyame region, had high amounts of antioxidant, phenol, flavonoid, anthocyanin and mineral. On the other hand, pomegranates from the Cuauhtémoc region had high amounts of protein, organic elements, and volatile compounds (alcohols, esters and aldehydes). Within these groups, the most abundant compounds were hexanol, (Z)-3-hexen-1-ol, hexanal, 4-Terpineol, α-Terpineol, and 2-Ethyl-1-hexanol. In the peel, high concentration of color, phenols, flavonoids, and antioxidants were observed. The extract of pomegranate juice had 59% anthocyanin content. The seeds had the highest mineral content. These results provide relevant information about the chemical properties of the pomegranate.

Raspberry production represents an alternative for farmers in the temperate zones of Mexico. Due to the environmental impact caused by conventional agriculture, there is currently greater demand for organic food in the national and international market. To achieve this need, new fertilization techniques based on organic amendments are being tested. In the present study, yields and quality were evaluated over a 3-year period (2015, 2016 and 2017) as well as the phytochemical compounds of the production in 2017, with management of organic versus conventional fertilization of raspberry crop in open field in Cuauhtémoc, Chihuahua, Mexico. During the three years of study, the conventional fertilization system obtained higher yields, compared to the organic one, with values of 2,698 and 2,351 g per linear meter in 2015, 2,423 and 1,301 g per linear meter in 2016 and the data for 2017 were 3,077 and 2,550 g per linear meter, respectively. Regarding quality, the results showed no statistical differences between the two systems of production about colour, firmness, total soluble solids, titratable acidity and pH of the fruit. Differently the composition of phytochemicals, phenols, flavonoids, total anthocyanins and antioxidant capacity was better under the organic management. In conclusion, the conventional system showed higher yields, the quality of the fruits was not different between the two systems, while the levels of phytochemicals were higher with the organic farming. Therefore, it is important to continue the research in order to improve soil fertility and achieve higher yields under the organic management.

Previous studies of Ganoderma lucidum have focused on its medicinal applications. Limited information is available about its antibacterial activity against plant pathogens. Thus, the goal of this study was to purify and characterize the antibacterial activity against plant pathogenic bacteria from culture fluids of G. lucidum. The nature of the bioactive components was determined using heat boiling, organic solvents, dialysis tubing, gel exclusion chromatography (GEC), proteinase sensitivity, HPLC, HPLC-APCI-MS, and GC-MS. The bioactive compounds were neither lipid, based on their solubility, nor proteic in nature, based on proteinase digestion and heat stability. The putative-bioactive polysaccharides have molecular weights that range from 3500 to 4500 Daltons as determined by dialysis tubing, GEC and APCI-MS analysis. The composition of the antibacterial compounds was determined by GC-MS. This is the first report of small polysaccharides produced by G. lucidum with activity against bacterial plant pathogens.