This systematic review examined, through the UTAUT2 model, the factors influencing the acceptance of artificial intelligence (AI) applications in university contexts. A total of 50 scientific texts published between 2018 and 2023 were analyzed and selected after a rigorous search of specialized databases. These findings confirm the versatility of UTAUT2 in elucidating technological adoption processes in higher education. Performance expectancy and hedonic motivation emerged as significant predictors of intentions and effective use among students, faculty, and administrative staff. Among students, perceived ease of use and social influence were also relevant. The analysis revealed differences in adoption patterns between STEM and non-STEM disciplines and between public and private institutions. Despite widespread positive perceptions of AI's potential, barriers such as distrust and lack of knowledge persist. The research also identified moderating and mediating factors, such as prior technology experience and technological self-efficacy. These results have important implications for the implementation of AI in higher education, suggesting the need for differentiated approaches according to the characteristics of each group and institutional context. It is recommended to develop strategies that address the identified barriers and leverage facilitators, with an emphasis on training, ethical design, and contextual adaptation of AI applications. Future research should explore the longitudinal evolution of these factors and examine AI adoption in non-STEM disciplines in greater depth.