A truss-floating aquaculture vessel is an innovative addition to the aquaculture industry, characterized by its large, porous, ship-shaped structure. It differs from traditional ships, offshore structures, and individual net cages. Due to its distinctive features, a large-scale truss-floating aquaculture vessel requires dedicated hydrodynamic and structural analysis, which is the primary focus of this paper. Our study starts with the calculation of wave loads acting on the vessel using the equivalent design wave method. Subsequently, it delves into the analysis of structural characteristics and stress distribution of the truss-floating aquaculture vessel, upon which structural optimization is performed. To determine the optimal design variables, a sensitivity analysis of the truss members is carried out using a parametric research method. Finally, the structure with multiple objectives is optimized using two distinct approaches: the adaptive simulated annealing algorithm (ASA) and the multi-island genetic algorithm (MIGA). The results reveal that prior to optimization, there is a risk of buckling and yielding damage occurring at various connections within the vessel structure. After optimization, the structural strength is significantly improved, accompanied by a reduction in the total weight of the vessel. This study offers a valuable reference for the design and structural safety assessment of this innovative truss-floating tank-type aquaculture vessel.