The developing central nervous system and the blood brain barrier are especially vulnerable and sensitive to different chemicals, including environmental contaminants and drugs. Developmental exposure to these compounds has been involved in several neurological disorders, such as autism spectrum disorders as well as Alzheimer’s and Parkinson’s diseases. Zebrafish (Danio Rerio) have emerged as powerful toxicological model systems that can speed up chemical hazard assessment and can be used to extrapolate neurotoxic effects that chemicals have on humans. Zebrafish embryos and larvae are convenient for high-throughput screening of chemicals, due to their small size, low-cost, easy husbandry, and transparency. Additionally, zebrafish are homologous to other higher order vertebrates in terms of molecular signaling processes, genetic compositions, and tissue/organ structures as well as neurodevelopment. This mini review underlines the potential of the zebrafish as complementary models for developmental neurotoxicity screening of chemicals and describes the different endpoints utilized for such screening with some studies illustrating their use.

Functionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake and low cytotoxicity. To date, the toxicological effects of f-CNOs on vertebrates have not been reported. In this study, the possible biological impact of f-CNOs on zebrafish during development is investigated, evaluating different toxicity end-points such as the survival rate, hatching rate, and heart beat rate. Furthermore, a bio-distribution study of boron dipyrromethene (BODIPY) functionalized CNOs in zebrafish larvae is performed by utilizing inverted selective plane illumination microscopy (iSPIM), due to its intrinsic capability of allowing for fast 3D imaging. Our in vivo findings indicate that f-CNOs exhibit no toxicity, good biocompatibility (in the concentration range of 5–100 μg mL⁻¹) and a homogenous biodistribution in zebrafish larvae.

Carbon nanomaterials (CNMs) are increasingly employed in nanomedicine as carriers for intracellular transport of drugs, imaging probes, and therapeutics agents, thanks to their unique optical and physicochemical properties. However, a better understanding about the effects of CNMs on a vertebrate model at the whole animal level is required. In this study, we compare the toxicity of oxidized carbon nano-onions (oxi-CNOs), oxidized carbon nano-horns (oxi-CNHs) and graphene oxide (GO) in zebrafish (Danio rerio). We evaluate the possible effects of these nanomaterials on zebrafish development by assessing different end-points and exposure periods.

This article discusses the potential of Zebrafish (ZF) (Danio Rerio), as a model for epilepsy research. Epilepsy is a neurological disorder affecting both children and adults, and many aspects of this disease are still poorly understood. In vivo and in vitro models derived from rodents are the most widely used for studying both epilepsy pathophysiology and novel drug treatments. However, researchers have recently obtained several valuable insights into these two fields of investigation by studying ZF. Despite the relatively simple brain structure of these animals, researchers can collect large amounts of data in a much shorter period and at lower costs compared to classical rodent models. This is particularly useful when a large number of candidate antiseizure drugs need to be screened, and ethical issues are minimized. In ZF, seizures have been induced through a variety of chemoconvulsants, primarily pentylenetetrazol (PTZ), kainic acid (KA), and pilocarpine. Furthermore, ZF can be easily genetically modified to test specific aspects of monogenic forms of human epilepsy, as well as to discover potential convulsive phenotypes in monogenic mutants. The article reports on the state-of-the-art and potential new fields of application of ZF research, including its potential role in revealing epileptogenic mechanisms, rather than merely assessing iatrogenic acute seizure modulation.

A new generation of fluorescent carbon nano-onions with enhanced solubility in biological media and bright photoluminescence is reported. The nano-onions functionalized with a water soluble boron dipyrromethene dye emit in the far red spectrum with a high quantum yield (Φ_F) and are suitable for high resolution imaging. The nanoparticles are characterized by a variety of different analytical techniques such as thermogravimetric analysis, dynamic light scattering, zeta potential, electron microscopy, Raman, X-ray photoelectron and fluorescence spectroscopies. They are easily internalized by human breast cancer cells (MCF-7) without any significant toxic effects. Moreover, confocal imaging studies show they exhibit a high fluorescence intensity and are localized in the lysosomes at a very low concentration. Our findings confirm the excellent potentialities of these functionalized carbon nanomaterials as biocompatible platform for high resolution biological imaging.