Katarzyna Pacyga

Dr. Katarzyna Pacyga

orcid

Research Focus & Metrics

Academic Career Length

12Years (2015-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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27 May 2019 in World Journal of Microbiology and Biotechnology
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KPKatarzyna Pacyga
Katarzyna Pacyga
Izabela MichalakIzabela Michalak
Izabela Michalak
PPPaweł Wiesław Pacyga
Paweł Wiesław Pacyga
SBS. Baśladyńska
S. Baśladyńska
KCK. Chojnacka
K. Chojnacka
In the present paper, products obtained from a blue-green microalga Spirulina platensis filtrate (applied for seed soaking and for foliar spray) and homogenate (used for seed coating) were tested in the cultivation of radish. Their effect on length, wet mass, multielemental composition and the greenness index of the radish leaves was examined. Multi-elemental analyses of the algal products, and radish were also performed using inductively coupled plasma-optical emission spectrometry (ICP-OES). The best soaking time, concentrations of filtrate and doses of homogenate were established. The longest and heaviest plants were observed for homogenate applied at a dose of 300 µL per 1.5 g of seeds and 15% of filtrate applied as foliar spray. The highest chlorophyll content was found in the group treated with 100 µL of homogenate and 5% of filtrate. In the case of soaking time, the longest plants were in the group where seeds were soaked for 6 h, but the heaviest and greenest were after soaking for 48 h. The applied algal products increased the content of elements in seedlings. Obtained results proved that algal extracts have high potential to be applied in modern horticulture and agriculture. The use of Spirulina-based products is consistent with the idea of sustainable agriculture that could help to ensure production of sufficient human food to meet the needs of rising population and protection of the environment.

1 January 2016 in BioMed Research International
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KPKatarzyna Pacyga
Katarzyna Pacyga
Izabela MichalakIzabela Michalak
Izabela Michalak
ŁTŁukasz Tuhy
Łukasz Tuhy
KCKatarzyna Chojnacka
Katarzyna Chojnacka
We explored two methods for obtaining aqueous extracts: boiling and soaking of Baltic seaweeds (EB and ES, resp.). Algal extracts were characterized in terms of polyphenols, micro- and macroelements, lipids content, and antibacterial properties. The utilitarian properties were examined in the germination tests onLepidium sativumfor three extract dilutions (0.5, 2.5, and 10%). It was found that the extracts were similar in micro- and macroelement concentrations. Water was proved to be a good solvent to extract phenolic compounds. The algal extract produced by soaking biomass did not show inhibitory effect onEscherichia coliandStaphylococcus aureus. Only the boiled extract had an inhibitory activity againstE. coli. Germination tests revealed a positive influence of the bioproducts on the cultivated plants. In the group treated with 10% EB, plants were 13% longer than in the control group; the content of elements B, Mo, Zn, and Na in the group treated with 10% ES was higher by 76%, 48%, 31%, and 59% than in the control group, respectively; the content of chlorophyll was 2.5 times higher in 0.5% ES than in the control group. Extracts showed the slight impact on the morphology of plants.

21 July 2023 in Molecules
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KPKatarzyna Pacyga
Katarzyna Pacyga
PPPaweł Wiesław Pacyga
Paweł Wiesław Pacyga
Agnieszka Barbara NajdaAgnieszka Barbara Najda
Agnieszka Barbara Najda
Izabela MichalakIzabela Michalak
Izabela Michalak
The aim of this publication is to present rapid screening methods (visual/colorimetric) that will enable quick identification of the presence of biologically active compounds in aqueous solutions. For this reason, 26 plant extracts obtained by ultrasound-assisted extraction were analysed for the content of these compounds. Higher plants, used as a raw material for extraction, are common in Europe and are easily available. The article proposes a comparison of various protocols for the identification of various compounds, e.g., phenolic compounds (phenols, tannins, anthocyanins, coumarins, flavones, flavonoids), vitamin C, quinones, quinines, resins, glycosides, sugars. Initial characterisation of the composition of plant extracts using fast and inexpensive methods allows you to avoid the use of time-consuming analyses with the use of advanced research equipment. In addition, the antioxidant activity of plant extracts using spectrophotometric methods (DPPH, ABTS, FRAP assay) and quantitative analysis of plant hormones such as abscisic acid, benzoic acid, gibberellic acid, indole acetic acid, jasmonic acid, salicylic acid, zeatin, zeatin riboside, and isipentenyl adenine was performed. The obtained results prove that the applied visual methods show different sensitivity in detecting the sought chemical compounds. Therefore, it is necessary to confirm the presence or absence of bioactive substances and their concentration using modern analytical methods.

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KPKatarzyna Pacyga
Katarzyna Pacyga
PPPaweł Wiesław Pacyga
Paweł Wiesław Pacyga
ETEwa Topola
Ewa Topola
Szymon ViscardiSzymon Viscardi
Szymon Viscardi
ADAnna Duda-Madej
Anna Duda-Madej
The rising prevalence of drug-resistant bacteria underscores the need to search for innovative and nature-based solutions. One of the approaches may be the use of plants that constitute a rich source of miscellaneous compounds with a wide range of biological properties. This review explores the antimicrobial activity of seven bioactives and their possible molecular mechanisms of action. Special attention was focused on the antibacterial properties of berberine, catechin, chelerythrine, cinnamaldehyde, ellagic acid, proanthocyanidin, and sanguinarine against Staphylococcus aureus, Enterococcus spp., Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Serratia marcescens and Pseudomonas aeruginosa. The growing interest in novel therapeutic strategies based on new plant-derived formulations was confirmed by the growing number of articles. Natural products are one of the most promising and intensively examined agents to combat the consequences of the overuse and misuse of classical antibiotics.

2 March 2020 in Sustainability
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KPKatarzyna Pacyga
Katarzyna Pacyga
Anita BiesiadaAnita Biesiada
Anita Biesiada
Izabela MichalakIzabela Michalak
Izabela Michalak
PPPaweł Wiesław Pacyga
Paweł Wiesław Pacyga
This research presents the possibility of using innovative botanical extracts as biostimulants of plant growth to improve plant nutritional value, growth, and development. It is important to increase agricultural production but this process should be carried out in a sustainable way, without causing risks for both the environment and consumers. For this reason, we have focused on the use of 14 natural raw materials and ultrasound assisted extraction for the production of biostimulants. Results proved that higher plants can be used to obtain valuable products for the application in modern horticulture and agriculture. For instance, extract based on Urtica dioica L. showed the highest biostimulatory properties: in the group sprayed with 0.1% extract cabbage seedlings were longer by 31%, while with 1.0% extract of Polygonum aviculare L. roots were longer by 72% than in the control group treated with water. Extracts based on Equisetum arvense L. (0.5%) and Urtica dioica L. (leaf) (0.1%) increased the fresh weight of sprouts by 113% and 112%, respectively. The highest root weight was observed in groups treated with Equisetum arvense L. (0.5%), Polygonum aviculare L. (0.5%), and Urtica dioica L. (leaf) (2.5%)—heavier by 207%, 206%, and 205%, respectively. Most of biostimulants increased the content of pigments involved in photosynthesis (e.g. 156% more chlorophyll for 0.1% Hypericum perforatum L. extract), decreased the content of polyphenols (e.g. 47% less for 2.5% Trifolium pretense extract), and showed a varied impact on antioxidant activity. There is an increasing interest in botanical extracts due to their high content of biologically active compounds and wide variety of application possibilities.

1 January 2020 in Process Biochemistry
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Izabela MichalakIzabela Michalak
Izabela Michalak
MMMałgorzata Mironiuk
Małgorzata Mironiuk
KPKatarzyna Pacyga
Katarzyna Pacyga
JTJustyna Trynda
Justyna Trynda
KMKrzysztof Marycz
Krzysztof Marycz