A review of indigenous Mexican plants with edible stems and leaves and their nutritional and nutraceutical potential was conducted, complemented by the authors’ experiences. In Mexico, more than 250 species with edible stems, leaves, vines and flowers, known as “quelites,” are collected or are cultivated and consumed. The assessment of the quelite composition depends on the chemical characteristics of the compounds being evaluated; the protein quality is a direct function of the amino acid content, which is evaluated by high-performance liquid chromatography (HPLC), and the contribution of minerals is evaluated by atomic absorption spectrometry, inductively coupled plasma-optical emission spectrometry (ICP-OES) or ICP mass spectrometry. The total contents of phenols, flavonoids, carotenoids, saponins and other general compounds have been analyzed using UV-vis spectrophotometry and by HPLC. For the determination of specific compounds such as phenolic compounds, flavonoids, organic acids and other profiles, it is recommended to use HPLC-DAD, UHPLC-DAD, UFLC-PDA or gas chromatography-mass spectrometry. The current biochemical analysis and biological evaluations were performed to understand the mechanisms of action that lead to decreased glucose levels and lipid peroxidation, increased hypoglycemic and antitumor activity, immune system improvement, increased antibacterial and antifungal activity and, in some cases, anti-Helicobacter pylori activity.

Phaseolus vulgaris L. is one of the most consumed and documented legumes in regard to its grain composition, but little is known about P. coccineus L. To evaluate and compare the phenolic compound content and antioxidant activity between landraces of P. coccineus and P. vulgaris, a total of 14 accessions of P. coccineus and P. vulgaris were collected from farmers in Oaxaca, Mexico. Based on reference standards and spectrophotometry, the polyphenol, flavonoid and anthocyanin contents were quantified, and the antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed significant differences (p ≤ 0.05) between species and accessions, where P. coccineus and P. vulgaris significantly differed in their contents of polyphenols, flavonoids, and anthocyanins, as well as their antioxidant activity in the seed coat and cotyledons. Higher concentrations were found in the seed coat than in the cotyledons for both species. P. vulgaris had a higher anthocyanin content in the seed coat and a higher flavonoid content in the cotyledons than P. coccineus, but it did not for the other compounds tested. There was high variability among the accessions that were classified into four phenotypic groups: Two of P. coccineus, one of a P. coccineus–vulgaris mixed group, and one group of P. vulgaris.

Mezcal is a traditional Mexican spirit produced by distilling fermented Agave. The effects of Agave species, origin, and season on the volatile compound profile were studied in mezcal from Oaxaca, Mexico. Liquid-liquid extraction was used to isolate volatile compounds from mezcals made from Agave angustifolia Haw. and Agave potatorum Zucc. These compounds were identified using gas chromatography-mass spectrometry (GC-MS). Eighty-four volatile compounds were identified, including alcohols, esters, fatty acids, ketones, furans, and others. Using variance analysis, it was possible to observe significant differences for the 26, 24, and 10 compounds in mezcal samples that differed based on Agave species, origin, and season. 3-Ethyl-phenol was identified only in samples of mezcal from A. angustifolia, and this volatile compound could be used as an authentic marker of mezcal from A. angustifolia (p ≤ 0.01).

The green bean is an important crop worldwide, because it is rich in protein, dietary fiber, vitamins, and minerals, as well as bioactive compounds that provide it with important functional properties; however, the composition of many landraces is still unknown. The purpose of this project was to characterize Phaselus vulgaris and coccineus L. landrace green beans on pH, titratable acidity, total soluble solids, total sugars, color parameters, total phenols, monomeric anthocyanins, and in vitro antioxidant activity (DPPH and FRAP). Regarding the content of total sugars, differences were registered between both species, as opposed to results observed in total soluble solids. Color parameters showed higher reddish tones for P. vulgaris landraces, though P. coccineus had a higher total phenolic content, especially the reddish landraces, which correlated directly to a higher antioxidant activity by DPPH and FRAP. In the protein content, the species P. vulgaris registered the highest content. These results could contribute to a greater use and even promote the genetic improvement of the outstanding pods that serve as one of the main food products in rural regions for higher benefits.

The excessive use of pesticides in agriculture causes problems related to public health; biodiversity; the contamination of water bodies, soil and air; and general environmental degradation, including interactions with climate change effects. The aim of this work was to describe the patterns of pesticide use in 17 Latin American countries and their associations with the total harvested area and the harvested areas of the five main crops on the basis of statistics reported by the Food and Agriculture Organization from 1990 to 2021. Principal component analysis (PCA) revealed four different patterns among countries according to the magnitude of pesticide application: Brazil (G1) > Argentina (G2) > Colombia and Mexico (G3) > Central and South America (G4). Together, more than 1.2 million tons of active ingredients is applied annually, carrying harmful effects and risks. In the joint analysis of both datasets (applied pesticides and harvested area), different correlations were observed between the amount of pesticides applied and the harvested area; for example, in G1 and G2, positive and significant correlations were detected between the applied amounts of total pesticides, herbicides, insecticides and bactericides and the total area harvested by the main crop, but in G3, there was a negative correlation with the five main crops.

Medicinal plants grown in natural settings are exposed to different adverse environmental conditions that determine their growth and development as well as the composition and concentration of secondary metabolites in their organs. The objective of this study was to evaluate the effects of environmental conditions associated with localities and annual growth cycles on the contents of phenolic compounds and flavonoids, antioxidant activity and potentially bioactive phenolic acids in the roots of Eryngium montanum, a medicinal species from temperate Mexico. The samples for composition analysis were collected using a bifactorial design: Factor A consisted of the localities (Morelos and La Unión de San Martin Huamelulpam, Mexico) and Factor B was represented by the annual growth cycle (2020 and 2021). In each sample, the contents of polyphenols and equivalent flavonoids of quercetin and catechin and antioxidant activity were evaluated using spectrophotometry. Subsequently, chlorogenic, caffeic and rosmarinic acids were identified and quantified using high-performance liquid chromatography with diode-array detection (HPLC-DAD). The annual growth conditions and, to a lesser extent, the locality of origin of the samples significantly influenced the contents of phenolic compounds and antioxidant activity. The environmental conditions that occurred in 2021 favored an increase in the contents of phenolic compounds compared to those in 2020, and the same pattern was observed for chlorogenic acid; however, for caffeic and rosmarinic acids, the opposite pattern was observed. The content of phenolic acids in the roots of E. montanum follows different and independent patterns between cycles based on the interaction between the locality of origin and annual growth cycle. This study quantifies the magnitude of the total environmental effect on the phenolic compound concentrations in E. montanum roots, which was measured via sampling during two annual growth cycles, where the sampling locations factor had little influence. The bioactive compounds identified in E. montanum roots have the potential for use as alternative medicines, as mentioned by different families from Oaxaca, Mexico.