Journal of Animal Science and Technology (JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science. Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare.

Environmental factors such as temperature, humidity, light, and CO₂ influence plant growth, and unfavorable environmental conditions cause stress in plants, producing symptoms in their early growth stages. The increasing importance of optimizing crop management strategies has led to a rising demand for the precise evaluation of stress symptoms during early plant growth. Advanced technologies are transforming plant health monitoring through enabling image-based stress analysis. Machine learning (ML) models can effectively identify the important features and morphological changes connected with various stress conditions through the use of large datasets acquired from high-resolution plant images. Therefore, the objective of this study was to develop a method for classifying the early-stage stress symptoms of pepper seedlings and enabling their identification and quantification using image processing and a support vector machine (SVM). Two-week-old pepper seedlings were grown under different temperatures (20, 25, and 30 °C), light intensity levels (50, 250, and 450 µmol m⁻²s⁻¹), and day–night hours (8/16, 10/14, and 16/8) in five controlled plant growth chambers. Images of the seedling canopies were captured daily using a low-cost red, green, and blue (RGB) camera over a two-week period. Eighteen color features, nine texture features using the gray-level co-occurrence matrix (GLCM), and one morphological feature were extracted from each image. A two-way ANOVA and multiple mean comparison (Duncan) analysis were used to determine the statistical significance of the treatment effects. To reduce feature overlap, sequential feature selection (SFS) was applied, and a support vector machine (SVM) was used for stress classification. The SFS method was used to identify the optimal features for the classification model, leading to substantial increases in stress classification accuracy. The SVM model, using these selected features, achieved a classification accuracy of 82% without the SFS and 86% with the SFS. To address overfitting, 5- and 10-fold cross-validation were used, resulting in MAEs of 0.138 and 0.163 for the polynomial kernel, respectively. The SVM model, evaluated with the ROC curve and confusion matrix, achieved a classification accuracy of 85%. This classification approach enables real-time stress monitoring, allowing growers to optimize environmental conditions and enhance seedling growth. Future directions include integrating this system into automated cultivation environments to enable continuous, efficient stress monitoring and response, further improving crop management and productivity.

The geometric feature characterization of fruit trees plays a role in effective management in orchards. LiDAR (light detection and ranging) technology for object detection enables the rapid and precise evaluation of geometric features. This study aimed to quantify the height, canopy volume, tree spacing, and row spacing in an apple orchard using a three-dimensional (3D) LiDAR sensor. A LiDAR sensor was used to collect 3D point cloud data from the apple orchard. Six samples of apple trees, representing a variety of shapes and sizes, were selected for data collection and validation. Commercial software and the python programming language were utilized to process the collected data. The data processing steps involved data conversion, radius outlier removal, voxel grid downsampling, denoising through filtering and erroneous points, segmentation of the region of interest (ROI), clustering using the density-based spatial clustering (DBSCAN) algorithm, data transformation, and the removal of ground points. Accuracy was assessed by comparing the estimated outputs from the point cloud with the corresponding measured values. The sensor-estimated and measured tree heights were 3.05 ± 0.34 m and 3.13 ± 0.33 m, respectively, with a mean absolute error (MAE) of 0.08 m, a root mean squared error (RMSE) of 0.09 m, a linear coefficient of determination (r²) of 0.98, a confidence interval (CI) of −0.14 to −0.02 m, and a high concordance correlation coefficient (CCC) of 0.96, indicating strong agreement and high accuracy. The sensor-estimated and measured canopy volumes were 13.76 ± 2.46 m³ and 14.09 ± 2.10 m³, respectively, with an MAE of 0.57 m³, an RMSE of 0.61 m³, an r² value of 0.97, and a CI of −0.92 to 0.26, demonstrating high precision. For tree and row spacing, the sensor-estimated distances and measured distances were 3.04 ± 0.17 and 3.18 ± 0.24 m, and 3.35 ± 0.08 and 3.40 ± 0.05 m, respectively, with RMSE and r² values of 0.12 m and 0.92 for tree spacing, and 0.07 m and 0.94 for row spacing, respectively. The MAE and CI values were 0.09 m, 0.05 m, and −0.18 for tree spacing and 0.01, −0.1, and 0.002 for row spacing, respectively. Although minor differences were observed, the sensor estimates were efficient, though specific measurements require further refinement. The results are based on a limited dataset of six measured values, providing initial insights into geometric feature characterization performance. However, a larger dataset would offer a more reliable accuracy assessment. The small sample size (six apple trees) limits the generalizability of the findings and necessitates caution in interpreting the results. Future studies should incorporate a broader and more diverse dataset to validate and refine the characterization, enhancing management practices in apple orchards.

LoRa-based sensor nodes may provide a reliable solution for wireless communication in orchard cultivation and smart farming, facilitating real-time environmental monitoring. However, the signal strength and data integrity can be affected by several factors, such as trees, terrain, weather, and nearby electrical devices. The objective of this study is to evaluate the impact of orchard trees on the performance of a LoRa sensor node under orchard conditions. A sensor node, built with a commercial LoRa transceiver and microcontroller unit (MCU), was paired with a single-channel gateway linked to an orchard irrigation system. Performance metrics such as the packet delivery ratio (PDR), received signal strength indicator (RSSI), and signal-to-noise ratio (SNR) were measured over a range of 20 to 120 m under open field conditions and in an orchard with trees averaging 3.12 and 4.36 m in height. Data were sent every 20 s using three spreading factors (SF8, SF10, and SF12) and stored as a CSV file in the MCU via a Python program. The results showed that the PDR remained consistently high (100%) under non-vegetative (open field) conditions. In the orchard under vegetative conditions, the PDR dropped significantly, with SF12 maintaining 100% only up to 120 m. For SF10, the packet delivery rates dropped to 45% at 80 m, while SF8 achieved 100% at 20 m but decreased to 52% at 40 m. SNR values also declined with an increase in distance, becoming largely undetectable beyond 40 m for SF8. These findings indicate that vegetation greatly impacts LoRa sensor node performance, reducing packet delivery and signal quality in orchards.

The health monitoring of vegetable and fruit plants, especially during the critical seedling growth stage, is essential to protect them from various environmental stresses and prevent yield loss. Different environmental stresses may cause similar symptoms, making visual inspection alone unreliable and potentially leading to an incorrect diagnosis and delayed corrective actions. This study aimed to address these challenges by proposing a segmented regression model and a Mask R-CNN model for detecting the initiation time and symptoms of nutrient stress in cucumber seedlings within a controlled environment. Nutrient stress was induced by applying two different treatments: an indicative nutrient deficiency with an electrical conductivity (EC) of 0 dSm⁻¹, and excess nutrients with a high-concentration nutrient solution and an EC of 6 dSm⁻¹. Images of the seedlings were collected using an automatic image acquisition system two weeks after germination. The early initiation of nutrient stress was detected using a segmented regression analysis, which analyzed morphological and textural features extracted from the images. For the Mask R-CNN model, 800 seedling images were annotated based on the segmented regression analysis results. Nutrient-stressed seedlings were identified from the initiation day to 4.2 days after treatment application. The Mask R-CNN model, implemented using ResNet-101 for feature extraction, leveraged transfer learning to train the network with a smaller dataset, thereby reducing the processing time. This study identifies the top projected canopy area (TPCA), energy, entropy, and homogeneity as prospective indicators of nutritional deficits in cucumber seedlings. The results from the Mask R-CNN model are promising, with the best-fit image achieving an F1 score of 93.4%, a precision of 93%, and a recall of 94%. These findings demonstrate the effectiveness of the integrated statistical and machine learning (ML) methods for the early and accurate diagnosis of nutrient stress. The use of segmented regression for initial detection, followed by the Mask R-CNN for precise identification, emphasizes the potential of this approach to enhance agricultural practices. By facilitating the early detection and accurate diagnosis of nutrient stress, this approach allows for quicker and more precise treatments, which improve crop health and productivity. Future research could expand this methodology to other crop types and field conditions to enhance image processing techniques, and researchers may also integrate real-time monitoring systems.

Understanding the diverse environmental influences on seedling growth is critical for maximizing yields. The need for a more comprehensive understanding of how various environmental factors affect seedling growth is required. Integrating sensor data and image processing techniques offers a promising approach to accurately detect stress symptoms and uncover hidden patterns, enhancing the comprehension of seedling responses to environmental factors. The objective of this study was to quantify environmental stress symptoms for six seedling varieties using image-extracted feature characteristics. Three sensors were used: an RGB camera for color, shape, and size information; a thermal camera for measuring canopy temperature; and a depth camera for providing seedling height from the image-extracted features. Six seedling varieties were grown under controlled conditions, with variations in temperature, light intensity, nutrients, and water supply, while daily automated imaging was conducted for two weeks. Key seedling features, including leaf area, leaf color, seedling height, and canopy temperature, were derived through image processing techniques. These features were then employed to quantify stress symptoms for each seedling type. The analysis of stress effects on the six seedling varieties revealed distinct responses to environmental stressors. Integration of color, size, and shape parameters established a visual hierarchy: pepper and pak choi seedlings showed a good response, cucumber seedlings showed a milder response, and lettuce and tomato seedlings displayed an intermediate response. Pepper and tomato seedlings exhibited a wide range of growth stress symptoms, at 13.00% to 83.33% and 2.96% to 70.01%, respectively, indicating considerable variability in their reactions to environmental stressors. The suggested classification approach provides valuable groundwork for advancing stress monitoring and enabling growers to optimize environmental conditions.