Using unmanned aerial vehicles (UAV) as platforms for light detection and ranging (LiDAR) sensors offers the efficient operation and advantages of active remote sensing; hence, UAV-LiDAR plays an important role in forest resource investigations. However, high-precision individual tree segmentation, in which the most appropriate individual tree segmentation method and the optimal algorithm parameter settings must be determined, remains highly challenging when applied to multiple forest types. This article compared the applicability of methods based on a canopy height model (CHM) and a normalized point cloud (NPC) obtained from UAV-LiDAR point cloud data. The watershed algorithm, local maximum method, point cloud-based cluster segmentation, and layer stacking were used to segment individual trees and extract the tree height parameters from nine plots of three forest types. The individual tree segmentation results were evaluated based on experimental field data, and the sensitivity of the parameter settings in the segmentation methods was analyzed. Among all plots, the overall accuracy F of individual tree segmentation was between 0.621 and 1, the average RMSE of tree height extraction was 1.175 m, and the RMSE% was 12.54%. The results indicated that compared with the CHM-based methods, the NPC-based methods exhibited better performance in individual tree segmentation; additionally, the type and complexity of a forest influence the accuracy of individual tree segmentation, and point cloud-based cluster segmentation is the preferred scheme for individual tree segmentation, while layer stacking should be used as a supplement in multilayer forests and extremely complex heterogeneous forests. This research provides important guidance for the use of UAV-LiDAR to accurately obtain forest structure parameters and perform forest resource investigations. In addition, the methods compared in this paper can be employed to extract vegetation indices, such as the canopy height, leaf area index, and vegetation coverage.

Aboveground biomass (AGB) of shrub communities in the desert is a basic quantitative characteristic of the desert ecosystem and an important index to measure ecosystem productivity and monitor desertification. An accurate and efficient method of predicting the AGB of a shrub community is essential for studying the spatial patterns and ecological functions of the desert region. Even though there are several entries in the literature on the AGB prediction of desert shrub communities using remote sensing data, the applicability and accuracy of airborne LiDAR data and prediction methods have not been well studied. We first extracted the elevation, density and intensity variables based on the airborne LiDAR, and then sample plot-level AGB prediction models were constructed using the parametric regression (nonlinear regression) and nonparametric methods (Random Forest, Support Vector Machine, K-Nearest Neighbor, Gradient Boosting Machine, and Multivariate adaptive regression splines). We evaluated accuracies of all the AGB prediction models we developed based on the fit statistics. Results showed that: (1) the elevation, density and intensity variables obtained from LiDAR point cloud data effectively predicted the AGB of the desert shrub community at a sample plot level, (2) the kappa coefficient of nonlinear mixed-effects (NLME) model obtained was 0.6977 with an improvement by 13% due to the random effects included into the model, and (3) the nonparametric model, such as Support Vector Machine showed the best fit statistics (R² = 0.8992), which is 28% higher than the NLME-model, and effectively reduced the heteroscedasticity. The AGB prediction model presented in this paper, which is based on the airborne LiDAR data and machine learning algorithm, will provide a valuable tool to the managers and researchers for evaluating desert ecosystem productivity and monitoring desertification.

Research on the inversion of forest aboveground biomass based on airborne light detection and ranging (LiDAR) data focuses on finding the relationship between the two, such as established linear or nonlinear models. However, these models may have poorer estimation accuracy for tree-components biomass and cannot guarantee the additivity of each component. Therefore, we aimed to develop an error-in-variable biomass model system that ensures both the compatibility of the individual tree component biomass with the diameter at breast height and the additivity of component biomass. The system we developed used the airborne LiDAR data and field-measured data of principis-rupprechtii (Larix gmelinii var.) trees, collected from north China. Our model system not only ensured the additivity of nonlinear biomass models, it also accounted for the impact of measurement errors. We first selected the airborne LiDAR-derived variable with the highest contribution to the biomass of each component and then developed an inversion model system with that variable as an independent variable and with the biomass of each component as the dependent variable using allometric functions. Moreover, two model estimation methods, two-stage error (TSEM) and nonlinear seemingly unrelated regression (NSUR) with one-step, two-step, and summation methods, were also applied, and their performances were compared. The results showed that both NSUR-one-step and TSEM-one-step led to similar parameter estimates and performance for a system, and the fitting accuracy of a model system was not very attractive. The variance function included in a model system reduced the heteroscedasticity effectively and improved the model accuracy. Overall, this study successfully combined the error-in-variable modeling with the airborne LiDAR data, proposed methods that can be used for the extension of component biomass from an individual tree to a stand and that might improve the estimation accuracy of carbon storage. A compatible model system can be further improved if various sources of error in the variables are identified, and their impacts on the system are effectively accounted for.

Desert vegetation is an important part of arid and semi-arid areas, which plays an important role in preventing wind and fixing sand, conserving water and soil, maintaining the balanced ecosystem. Therefore, mapping the vegetation accurately is necessary to conserve rare desert plants in the fragile ecosystems that are easily damaged and slow to recover. In mapping desert vegetation, there are some weaknesses by using traditional digital classification algorithms from high resolution data. The traditional approach is to use spectral features alone, without spatial information. With the rapid development of drones, cost-effective visible light data is easily available, and the data would be non-spectral but with spatial information. In this study, a method of mapping the desert rare vegetation was developed based on the pixel classifiers and use of Random Forest (RF) algorithm with the feature of VDVI and texture. The results indicated the accuracy of mapping the desert rare vegetation were different with different methods and the accuracy of the method proposed was higher than the traditional method. The most commonly used decision rule in the traditional method, named Maximum Likelihood classifier, produced overall accuracy (76.69%). The inclusion of texture and VDVI features with RGB (Red Green Blue) data could increase the separability, thus improved the precision. The overall accuracy could be up to 84.19%, and the Kappa index with 79.96%. From the perspective of features, VDVI is less important than texture features. The texture features appeared more important than spectral features in desert vegetation mapping. The RF method with the RGB+VDVI+TEXTURE would be better method for desert vegetation mapping compared with the common method. This study is the first attempt of classifying the desert vegetation based on the RGB data, which will help to inform management and conservation of Ulan Buh desert vegetation.

Rhododendron longipedicellatum is a narrow endemic species and a subject of urgent demand in the domestic market and overseas. Its fascinating shapes, brilliantly gilvous flowers, and unusual flowering time endow this species with extremely high ornamental value. However, only five wild populations of R. longipedicellatum surviving in limestone habitat have been found through elaborate field investigation, and the number of the populations decreases further or is even confronted with risk of extinction due to the damage of human activities. To enhance the protection and utilization of R. longipedicellatum, this study systematically investigated several important aspects of reproductive biology, including floral syndrome, pollen viability and stigma receptivity, petal color reflectance, breeding system, and pollination biology. The results demonstrated that arched styles not only create obvious herkogamy that avoide self-pollination, but also effectively reduce rain damage to the intrinsic characteristics of the stigma surface secretions, promoting the female fitness of R. longipedicellatum in poor weather. Pollen viability maintained a high level over the flowering period. The reflectance spectrum of petals had two peaks at wavelengths of 360 and 580 nm. Tests of OCI, P/O and artificial pollination all indicated that R. longipedicellatum was self-compatible and that the breeding system was mixed mating. Geitonogamy mediated by Bombus braccatus was the primary pollination route in the natural environment, which suggested that the breeding system of R. longipedicellatum might be evolving from selfing to outcrossing. The pollination vector of R. longipedicellatum was very specific, in that only B. braccatus was confirmed to deliver pollen to the stigmas. Visitation frequency was influenced by the activity rhythms and resource requirements of the different castes (i.e., sex). B. braccatus workers were the most effective pollinators because of higher visitation frequency and more effective contribution to fruit production, whereas the presence of B. braccatus males might enhance pollen flow within the population to a certain extent. Finally, these findings not only provided a reliable theoretical basis for hybridization breeding of R. longipedicellatum as parents, but also laid a solid foundation for further molecular biology studies to more broadly reveal the mechanisms of its endangerment in the future.

Rapidly advancing airborne laser scanning technology has become greatly useful to estimate tree- and stand-level variables at a large scale using high spatial resolution data. Compared with that of ground measurements, the accuracy of the inferred information of diameter at breast height (DBH) from a remotely sensed database and the models developed with traditional regression approaches (e.g., ordinary least square regression) may not be sufficient. Thus, this regression approach is no longer appropriate to develop accurate models and predict DBH from remotely sensed-related variables because DBH is subject to the random effects of forest stands. This study developed a generalized nonlinear mixed-effects DBH estimation model from remotely sensed imagery data. The light detection and ranging (LiDAR)-derived stand canopy density, crown projection area, and tree height were used as predictors in the DBH estimation model. These variables can be more readily measured over an extensive forest area with higher accuracy compared to the conventional field-based methods. The airborne LiDAR data for a total of 402 Picea crassifolia Kom trees on a sample plot that were divided into 16 sub-sample plots and located in the most important distribution region of western China were used. The leave-one sub-sample plot-out cross-validation method was applied to evaluate the model’s prediction accuracy. The results indicated that the random effects of the sub-sample plot on the prediction of DBH were large and their inclusion into the DBH model significantly improved the prediction accuracy. The prediction accuracy of the proposed model at the mean (M) response was also substantially improved relative to the accuracy obtained from the base model. Among several tree selection alternatives evaluated, a sample size of the two largest trees per sub-sample plot used in estimating the random effects showed a significantly higher accuracy compared to other sampling alternatives. This sample size would balance both the measurement cost and potential prediction errors. The nonlinear mixed-effects DBH estimation model at the M response can also be applied if obtaining the estimates of individual tree DBH with a relatively lower cost, and a lower prediction accuracy was the purpose of the study.