We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of Trichinella spiralis (T. spiralis) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 T. spiralis strain. Tongues and diaphragms were obtained daily from days 13 to 39 post infection. Diaphragms were compressed and subjected to Giemsa stain. Tongues were histologically sectioned and stained with erythrosine B or hematoxylin and eosin. The cellular infiltrate and the nurse cell-larva complex were detected by optical microscopy since day 16 post infection. The size of the larva increased exponentially during the course of the infection. The kinetics of eosinophils showed a multimodal trend, with a bimodal predominance. The maximum peaks were reached on days 21 and 27 post infection. The results of this study demonstrate that eosinophils occur abundantly in two transcendent moments of the T. spiralis life cycle: first, when the stage 1 larva invades the myocyte and second when the nurse cell-larva complex has been fully developed. These results help one to understand the immunobiology of T. spiralis, highlighting the importance of eosinophils in the survival of the larva in skeletal muscle. Further studies are needed to characterize the cell populations that comprise the cellular infiltrate during the development of the mother cell.

This study evaluated the potential of Nanche seeds as a bio-adsorbent for removing TA from aqueous solutions. Additionally, we inferred the interaction mechanisms involved in the adsorption process from physicochemical evidence, isotherm modelling, and ADI analysis. The Langmuir model best fit the experimental data (R² = 0.999) across temperatures (20–60 °C). Thermodynamically, the process was endothermic and spontaneous, (∆H° = 10.11 ± 0.45 kJ/mol and ∆G° = −28.58 ± 1.03 kJ/mol), indicating that the interaction mechanisms were physical. The value of ∆S° (0.116 ± 0.003 kJ/mol·K), the Freundlich constant (n > 1), and data derived from ADI analysis of SEM micrographs that support the increased complexity and multifractal nature of the process. FTIR revealed surface changes in the adsorbent material attributable to the interactions between the seed and the TA. Extent of vibrations associated with carboxyl groups decreased after adsorption, which was attributed to low-energy interactions, such as π-π stacking, supporting physisorption interaction. The ki values increased linearly with temperature and TA concentration. This study used ideal adsorption systems to elucidate fundamental mechanisms, providing a foundation for future, more complex research. Nance seeds proved to be effective yet underutilized adsorbent material. Further studies are needed on its applicability in continuous processes and multicomponent matrices systems.