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Sediment pollution is a major issue in coastal areas, potentially endangering human health and the marine environments. We investigated the short-term sublethal effects of sediments contaminated with polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) on the sea urchin Paracentrotus lividus for two months. Spiking occurred at concentrations below threshold limit values permitted by the law (TLVPAHs = 900 µg/L, TLVPCBs = 8 µg/L, Legislative Italian Decree 173/2016). A multi-endpoint approach was adopted, considering both adults (mortality, bioaccumulation and gonadal index) and embryos (embryotoxicity, genotoxicity and de novo transcriptome assembly). The slight concentrations of PAHs and PCBs added to the mesocosms were observed to readily compartmentalize in adults, resulting below the detection limits just one week after their addition. Reconstructed sediment and seawater, as negative controls, did not affect sea urchins. PAH- and PCB-spiked mesocosms were observed to impair P. lividus at various endpoints, including bioaccumulation and embryo development (mainly PAHs) and genotoxicity (PAHs and PCBs). In particular, genotoxicity tests revealed that PAHs and PCBs affected the development of P. lividus embryos deriving from exposed adults. Negative effects were also detected by generating a de novo transcriptome assembly and its annotation, as well as by real-time qPCR performed to identify genes differentially expressed in adults exposed to the two contaminants. The effects on sea urchins (both adults and embryos) at background concentrations of PAHs and PCBs below TLV suggest a need for further investigations on the impact of slight concentrations of such contaminants on marine biota.
Luisa Albarano; Valerio Zupo; Davide Caramiello; Maria Toscanesi; Marco Trifuoggi; Marco Guida; Giovanni Libralato; Maria Costantini. Sub-Chronic Effects of Slight PAH- and PCB-Contaminated Mesocosms in Paracentrotus lividus Lmk: A Multi-Endpoint Approach and De Novo Transcriptomic. International Journal of Molecular Sciences 2021, 22, 6674 .
AMA StyleLuisa Albarano, Valerio Zupo, Davide Caramiello, Maria Toscanesi, Marco Trifuoggi, Marco Guida, Giovanni Libralato, Maria Costantini. Sub-Chronic Effects of Slight PAH- and PCB-Contaminated Mesocosms in Paracentrotus lividus Lmk: A Multi-Endpoint Approach and De Novo Transcriptomic. International Journal of Molecular Sciences. 2021; 22 (13):6674.
Chicago/Turabian StyleLuisa Albarano; Valerio Zupo; Davide Caramiello; Maria Toscanesi; Marco Trifuoggi; Marco Guida; Giovanni Libralato; Maria Costantini. 2021. "Sub-Chronic Effects of Slight PAH- and PCB-Contaminated Mesocosms in Paracentrotus lividus Lmk: A Multi-Endpoint Approach and De Novo Transcriptomic." International Journal of Molecular Sciences 22, no. 13: 6674.
Antihypertensive drugs are among the most prescribed drugs. Olmesartan acid, of the sartan class, belongs to a relatively new generation of antihypertensive drugs called angiotensin II receptor blockers. There are very few studies on the presence and fate of sartans in the environment, despite them being marketed in huge quantities, metabolized in low percentages, and detected in wastewater and water bodies. This paper presents a study on the less abundant and more polar fractions that have been neglected in previous studies, which led to the isolation by chromatographic methods of thirteen degradation byproducts (DPs), six of which are new, identified by nuclear magnetic resonance and mass spectrometry. A mechanism of degradation from the parent drug was proposed. The ecotoxicity of olmesartan acid and identified compounds was evaluated in Aliivibrio fischeri bacteria and Raphidocelis subcapitata algae to assess acute and chronic toxicity. For 75% of the DPs, acute and chronic exposure to the compounds, at concentrations of 5 mg/L, inhibited population growth in the algae and decreased bioluminescence in the bacteria.
Giovanni Luongo; Antonietta Siciliano; Giovanni Libralato; Marco Guida; Lorenzo Saviano; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. Complete Characterization of Degradation Byproducts of Olmesartan Acid, Degradation Pathway, and Ecotoxicity Assessment. Applied Sciences 2021, 11, 5393 .
AMA StyleGiovanni Luongo, Antonietta Siciliano, Giovanni Libralato, Marco Guida, Lorenzo Saviano, Lucio Previtera, Giovanni Di Fabio, Armando Zarrelli. Complete Characterization of Degradation Byproducts of Olmesartan Acid, Degradation Pathway, and Ecotoxicity Assessment. Applied Sciences. 2021; 11 (12):5393.
Chicago/Turabian StyleGiovanni Luongo; Antonietta Siciliano; Giovanni Libralato; Marco Guida; Lorenzo Saviano; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. 2021. "Complete Characterization of Degradation Byproducts of Olmesartan Acid, Degradation Pathway, and Ecotoxicity Assessment." Applied Sciences 11, no. 12: 5393.
In recent years, many studies have reported the frequent detection of antihypertensive agents such as sartans (olmesartan, valsartan, irbesartan and candesartan) in the influents and effluents of wastewater treatment plants (WWTPs) and in the superficial waters of rivers and lakes in both Europe and North America. In this paper, the degradation pathway for candesartan (CAN) was investigated by simulating the chlorination process that is normally used to reduce microbial contamination in a WWTP. Twelve isolated degradation byproducts (DPs), four of which were isolated for the first time, were separated on a C-18 column by employing a gradient HPLC method, and their structures were identified by combining nuclear magnetic resonance and mass spectrometry and comparing the results with commercial standards. On the basis of these results, a mechanism of formation starting from the parent drug is proposed. The ecotoxicity of CAN and its DPs was studied by conducting a battery of ecotoxicity tests; bioassays were performed using Aliivibrio fischeri (bacterium), Daphnia magna (planktonic crustacean) and Raphidocelis subcapitata (alga). The ecotoxicity results shed new light on the increased toxicity of DPs compared with the parent compound.
Giovanni Luongo; Lorenzo Saviano; Giovanni Libralato; Marco Guida; Antonietta Siciliano; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. Secondary Effects of Hypochlorite Treatment on the Emerging Pollutant Candesartan: The Formation of Degradation Byproducts and Their Toxicological Profiles. Molecules 2021, 26, 3422 .
AMA StyleGiovanni Luongo, Lorenzo Saviano, Giovanni Libralato, Marco Guida, Antonietta Siciliano, Lucio Previtera, Giovanni Di Fabio, Armando Zarrelli. Secondary Effects of Hypochlorite Treatment on the Emerging Pollutant Candesartan: The Formation of Degradation Byproducts and Their Toxicological Profiles. Molecules. 2021; 26 (11):3422.
Chicago/Turabian StyleGiovanni Luongo; Lorenzo Saviano; Giovanni Libralato; Marco Guida; Antonietta Siciliano; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. 2021. "Secondary Effects of Hypochlorite Treatment on the Emerging Pollutant Candesartan: The Formation of Degradation Byproducts and Their Toxicological Profiles." Molecules 26, no. 11: 3422.
Significant release of rare earth elements (REEs) into the environment is mainly due to active or abandoned mining sites, but their presence is globally increasing due to their use in several industrial sectors. The effects on primary producers as Raphidocelis subcapitata are still limited. This research focused on La and Ce as the two most widespread REEs that can be currently found up to hundreds of μg/L in water and wastewater. Microalgae were exposed to La and Ce for 3 days (pH = 7.8) (short-term exposure) to derive the effective concentrations inhibiting the growth on 10% (EC10) of the exposed population. EC10 values (0.5 mg/L of La and 0.4 mg/L of Ce) were used for the 28 days long-term exposure (renewal test) to observe after 7, 14, 21, and 28 days on a multi-endpoint basis microalgae growth inhibition (GI), biomarkers of stress (reactive oxygen species (ROS), superoxide dismutase (SOD), and catalase (CAT)), and bioconcentration. Results evidenced that La and Ce EC10 increased GI (day 28) up to 38% and 28%, respectively. ROS, CAT, and SOD activities showed differential responses from day 7 to day 14, 21, and 28, suggesting, in most of the cases, that La and Ce effects were counteracted (i.e., being the values at day 28 not significantly different, p > 0.05, from the relative negative controls), except for La-related ROS activities. La and Ce significantly bioconcentrated in microalgae populations up to 2- and 5-fold (i.e., at day 28 compared to day 7), in that order. Bioconcentrated La and Ce were up to 3157 and 1232 μg/g dry weight (day 28), respectively. These results suggested that low La and Ce concentrations can be slightly toxic to R. subcapitata having the potential to be bioaccumulated and potentially transferred along the food web.
Antonietta Siciliano; Marco Guida; Sara Serafini; Maria Micillo; Emilia Galdiero; Simona Carfagna; Giovanna Salbitani; Franca Tommasi; Giusy Lofrano; Edith Guadalupe Padilla Suarez; Isidora Gjata; Antonios Apostolos Brouziotis; Marco Trifuoggi; Renato Liguori; Marco Race; Massimiliano Fabbricino; Giovanni Libralato. Long-term multi-endpoint exposure of the microalga Raphidocelis subcapitata to lanthanum and cerium. Science of The Total Environment 2021, 790, 148229 .
AMA StyleAntonietta Siciliano, Marco Guida, Sara Serafini, Maria Micillo, Emilia Galdiero, Simona Carfagna, Giovanna Salbitani, Franca Tommasi, Giusy Lofrano, Edith Guadalupe Padilla Suarez, Isidora Gjata, Antonios Apostolos Brouziotis, Marco Trifuoggi, Renato Liguori, Marco Race, Massimiliano Fabbricino, Giovanni Libralato. Long-term multi-endpoint exposure of the microalga Raphidocelis subcapitata to lanthanum and cerium. Science of The Total Environment. 2021; 790 ():148229.
Chicago/Turabian StyleAntonietta Siciliano; Marco Guida; Sara Serafini; Maria Micillo; Emilia Galdiero; Simona Carfagna; Giovanna Salbitani; Franca Tommasi; Giusy Lofrano; Edith Guadalupe Padilla Suarez; Isidora Gjata; Antonios Apostolos Brouziotis; Marco Trifuoggi; Renato Liguori; Marco Race; Massimiliano Fabbricino; Giovanni Libralato. 2021. "Long-term multi-endpoint exposure of the microalga Raphidocelis subcapitata to lanthanum and cerium." Science of The Total Environment 790, no. : 148229.
The removal of contaminants of emerging concern from urban wastewater treatment plants (WWTPs) remains a challenge to promote safe wastewater reuse practices. Macrolides are the most abundant antibiotics detected in untreated wastewater and their concentration in WWTPs effluents is only partially reduced by conventional treatments. Among several advanced oxidation processes (AOPs), photocatalysis has demonstrated the capability to effectively remove pharmaceuticals from different aqueous matrices. Recently, ZnO has emerged as an efficient, promising, and less expensive alternative to TiO2, due to its photocatalytic capability and attitude to exploit better the solar spectrum than TiO2. In this study, the behaviors of ZnO photocatalysis were evaluated using a representative macrolide antibiotic, spiramycin (SPY), in aqueous solutions and urban wastewater. After 80 min of photocatalysis, 95–99% removal of SPY was achieved at 1 g L−1 ZnO concentrations in aqueous solutions and wastewater, respectively. After treatment, the effluent toxicity, evaluated using the bacterium Aliivibrio fischeri, the green alga Raphidocelis subcapitata, and the crustacean Daphnia magna ranged between slight acute and high acute hazard. Filterable and ultrafilterable Zn concentrations were quantified in treated effluents and shown to be high enough to contribute to the observed toxicity.
Davide Vignati; Giusy Lofrano; Giovanni Libralato; Marco Guida; Antonietta Siciliano; Federica Carraturo; Maurizio Carotenuto. Photocatalytic ZnO-Assisted Degradation of Spiramycin in Urban Wastewater: Degradation Kinetics and Toxicity. Water 2021, 13, 1051 .
AMA StyleDavide Vignati, Giusy Lofrano, Giovanni Libralato, Marco Guida, Antonietta Siciliano, Federica Carraturo, Maurizio Carotenuto. Photocatalytic ZnO-Assisted Degradation of Spiramycin in Urban Wastewater: Degradation Kinetics and Toxicity. Water. 2021; 13 (8):1051.
Chicago/Turabian StyleDavide Vignati; Giusy Lofrano; Giovanni Libralato; Marco Guida; Antonietta Siciliano; Federica Carraturo; Maurizio Carotenuto. 2021. "Photocatalytic ZnO-Assisted Degradation of Spiramycin in Urban Wastewater: Degradation Kinetics and Toxicity." Water 13, no. 8: 1051.
Due to the continuous development, production and consumption of nanoparticles (NPs), their release, fate and effects in marine coastal environment can represent a major concern. The aim of this study was to evaluate the toxicity of ZnO nanoparticles (ZnO NPs) and compare it to bulk ZnSO4 on three macroinvertebrates: the isopod Cymodoce truncata (i.e. used for the first time in ecotoxicology), the amphipod Gammarus aequicauda and the sea urchin Paracentrotus lividus. This study showed concentration- and time-dependent relationships for all biological models for both ZnO NPs and ZnSO4. Both Zn forms elicited high toxicity to G. aequicauda and C. truncata juveniles, but ZnO NPs induced comparable responses to both species (96h-LC50 = 0.30 and 0.37 mg/L for G. aequicauda and C. truncata, respectively; p > 0.05), while differences were found after ZnSO4 exposure (96h-LC50 = 0.28 and 0.63 mg/L, respectively; p < 0.05). ZnO NPs generated sub-lethal effects on P. lividus embryos (72h-EC50 = 0.04 (0.03, 0.05) mg/L), not significantly different from ZnSO4 ones (72h-EC50 = 0.06 (0.05, 0.07) mg/L). Effects of ZnO NPs were similar to existing literature data for other testing species. C. truncata can be considered as a promising new biological model in (nano)ecotoxicology. Graphical abstract
Ermelinda Prato; Adele Fabbrocini; Giovanni Libralato; Luciana Migliore; Isabella Parlapiano; Raffaele D’Adamo; Alice Rotini; Loredana Manfra; Giusy Lofrano; Federica Carraturo; Marco Trifuoggi; Francesca Biandolino. Comparative toxicity of ionic and nanoparticulate zinc in the species Cymodoce truncata, Gammarus aequicauda and Paracentrotus lividus. Environmental Science and Pollution Research 2021, 28, 42891 -42900.
AMA StyleErmelinda Prato, Adele Fabbrocini, Giovanni Libralato, Luciana Migliore, Isabella Parlapiano, Raffaele D’Adamo, Alice Rotini, Loredana Manfra, Giusy Lofrano, Federica Carraturo, Marco Trifuoggi, Francesca Biandolino. Comparative toxicity of ionic and nanoparticulate zinc in the species Cymodoce truncata, Gammarus aequicauda and Paracentrotus lividus. Environmental Science and Pollution Research. 2021; 28 (31):42891-42900.
Chicago/Turabian StyleErmelinda Prato; Adele Fabbrocini; Giovanni Libralato; Luciana Migliore; Isabella Parlapiano; Raffaele D’Adamo; Alice Rotini; Loredana Manfra; Giusy Lofrano; Federica Carraturo; Marco Trifuoggi; Francesca Biandolino. 2021. "Comparative toxicity of ionic and nanoparticulate zinc in the species Cymodoce truncata, Gammarus aequicauda and Paracentrotus lividus." Environmental Science and Pollution Research 28, no. 31: 42891-42900.
Plastic is a ubiquitous material in our life, and its durability represents a great problem for the environment. Several studies reported the occurrence of plastic litter in different environmental compartments and, consequently, numerous efforts are currently focused on how improving its recycling process and produce environmentally friendly solutions. In recent years, biodegradable polymers/plastics (BPs) have been proposed to reduce environmental impacts in specific applications (e.g., when conventional plastics are difficult or expensive to remove from the environment). Their wide use in commercial products, especially in the packaging sector, is causing new pollution alarm. Research studies are ongoing to improve BPs manufacturing and characteristics, but few data are reported about their behavior and toxicity into the marine environment. This paper reviewed the current state of the art highlighting that, even though the degradation of BPs in simulated or real marine environments is quite investigated, only eleven papers reported their effects on marine organisms (e.g., behavioral and oxidative stress and potential cascading effects on marine ecosystems). Presently, the main benefits of BPs are linked to waste management (including collection and recycling of organic waste). Due to the existing knowledge gaps, BPs cannot be deemed yet as a solution to marine plastic pollution.
Loredana Manfra; Vincenzo Marengo; Giovanni Libralato; Maria Costantini; Francesca De Falco; Mariacristina Cocca. Biodegradable polymers: A real opportunity to solve marine plastic pollution? Journal of Hazardous Materials 2021, 416, 125763 .
AMA StyleLoredana Manfra, Vincenzo Marengo, Giovanni Libralato, Maria Costantini, Francesca De Falco, Mariacristina Cocca. Biodegradable polymers: A real opportunity to solve marine plastic pollution? Journal of Hazardous Materials. 2021; 416 ():125763.
Chicago/Turabian StyleLoredana Manfra; Vincenzo Marengo; Giovanni Libralato; Maria Costantini; Francesca De Falco; Mariacristina Cocca. 2021. "Biodegradable polymers: A real opportunity to solve marine plastic pollution?" Journal of Hazardous Materials 416, no. : 125763.
The awareness of citizens concerning the health risks caused by environmental pollution is growing, but studies on determinants of pro-environmental behaviors have rarely examined health-related aspects. In this study, we investigated these determinants using data from a large survey among Italian university students (15 Universities: 4778 filled questionnaires). Besides the health-related aspects, represented by environmental health risk perception and functional health literacy, we considered social and demographic characteristics (gender, area of residence, sources of information, trust in institutional and non-institutional subjects, and students’ capacity of positive actions, indicated as internal locus of control). The attitudes towards pro-environmental behaviors were positive for more than 70% of students and positively related with health risk perception, internal locus of control, and health literacy. The correspondence between the positive attitudes towards pro-environmental behaviors and the real adoption of such behaviors was approximately 20% for most behaviors, except for the separate collection of waste (60%). Such a discrepancy can be attributable to external obstacles (i.e., lack of time, costs, lack of support). The health-related aspects were linked to the pro-environmental attitudes, but to a lesser extent to pro-environmental behaviors, owing to the complexity of their determinants. However, they should be taken in account in planning education interventions.
Annalaura Carducci; Maria Fiore; Antonio Azara; Guglielmo Bonaccorsi; Martina Bortoletto; Giuseppina Caggiano; Andrea Calamusa; Antonella De Donno; Osvalda De Giglio; Marco Dettori; Pamela Di Giovanni; Angela Di Pietro; Alessio Facciolà; Ileana Federigi; Iolanda Grappasonni; Alberto Izzotti; Giovanni Libralato; Chiara Lorini; Maria Montagna; Liberata Nicolosi; Grazia Paladino; Giacomo Palomba; Fabio Petrelli; Tiziana Schilirò; Stefania Scuri; Francesca Serio; Marina Tesauro; Marco Verani; Marco Vinceti; Federica Violi; Margherita Ferrante. Pro-Environmental Behaviors: Determinants and Obstacles among Italian University Students. International Journal of Environmental Research and Public Health 2021, 18, 3306 .
AMA StyleAnnalaura Carducci, Maria Fiore, Antonio Azara, Guglielmo Bonaccorsi, Martina Bortoletto, Giuseppina Caggiano, Andrea Calamusa, Antonella De Donno, Osvalda De Giglio, Marco Dettori, Pamela Di Giovanni, Angela Di Pietro, Alessio Facciolà, Ileana Federigi, Iolanda Grappasonni, Alberto Izzotti, Giovanni Libralato, Chiara Lorini, Maria Montagna, Liberata Nicolosi, Grazia Paladino, Giacomo Palomba, Fabio Petrelli, Tiziana Schilirò, Stefania Scuri, Francesca Serio, Marina Tesauro, Marco Verani, Marco Vinceti, Federica Violi, Margherita Ferrante. Pro-Environmental Behaviors: Determinants and Obstacles among Italian University Students. International Journal of Environmental Research and Public Health. 2021; 18 (6):3306.
Chicago/Turabian StyleAnnalaura Carducci; Maria Fiore; Antonio Azara; Guglielmo Bonaccorsi; Martina Bortoletto; Giuseppina Caggiano; Andrea Calamusa; Antonella De Donno; Osvalda De Giglio; Marco Dettori; Pamela Di Giovanni; Angela Di Pietro; Alessio Facciolà; Ileana Federigi; Iolanda Grappasonni; Alberto Izzotti; Giovanni Libralato; Chiara Lorini; Maria Montagna; Liberata Nicolosi; Grazia Paladino; Giacomo Palomba; Fabio Petrelli; Tiziana Schilirò; Stefania Scuri; Francesca Serio; Marina Tesauro; Marco Verani; Marco Vinceti; Federica Violi; Margherita Ferrante. 2021. "Pro-Environmental Behaviors: Determinants and Obstacles among Italian University Students." International Journal of Environmental Research and Public Health 18, no. 6: 3306.
The discovery of various sartans, which are among the most used antihypertensive drugs in the world, is increasingly frequent not only in wastewater but also in surface water and, in some cases, even in drinking or groundwater. In this paper, the degradation pathway of olmesartan acid, one of the most used sartans, was investigated by simulating the chlorination process normally used in a wastewater treatment plant to reduce similar emerging pollutants. The structures of nine isolated degradation byproducts (DPs), eight of which were isolated for the first time, were separated via chromatography column and HPLC methods, identified by combining nuclear magnetic resonance and mass spectrometry, and justified by a proposed mechanism of formation beginning from the parent drug. Ecotoxicity tests on olmesartan acid and its nine DPs showed that 50% of the investigated byproducts inhibited the target species Aliivibrio fischeri and Raphidocelis subcapitata, causing functional decreases of 18% and 53%, respectively.
Giovanni Luongo; Antonietta Siciliano; Giovanni Libralato; Sara Serafini; Lorenzo Saviano; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. LC and NMR Studies for Identification and Characterization of Degradation Byproducts of Olmesartan Acid, Elucidation of Their Degradation Pathway and Ecotoxicity Assessment. Molecules 2021, 26, 1769 .
AMA StyleGiovanni Luongo, Antonietta Siciliano, Giovanni Libralato, Sara Serafini, Lorenzo Saviano, Lucio Previtera, Giovanni Di Fabio, Armando Zarrelli. LC and NMR Studies for Identification and Characterization of Degradation Byproducts of Olmesartan Acid, Elucidation of Their Degradation Pathway and Ecotoxicity Assessment. Molecules. 2021; 26 (6):1769.
Chicago/Turabian StyleGiovanni Luongo; Antonietta Siciliano; Giovanni Libralato; Sara Serafini; Lorenzo Saviano; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. 2021. "LC and NMR Studies for Identification and Characterization of Degradation Byproducts of Olmesartan Acid, Elucidation of Their Degradation Pathway and Ecotoxicity Assessment." Molecules 26, no. 6: 1769.
In recent years, many studies have highlighted the consistent finding of amoxicillin in waters destined for wastewater treatment plants, in addition to superficial waters of rivers and lakes in both Europe and North America. In this paper, the amoxicillin degradation pathway was investigated by simulating the chlorination process normally used in a wastewater treatment plant to reduce similar emerging pollutants at three different pH values. The structures of 16 isolated degradation byproducts (DPs), one of which was isolated for the first time, were separated on a C-18 column via a gradient HPLC method. Combining mass spectrometry and nuclear magnetic resonance, we then compared commercial standards and justified a proposed formation mechanism beginning from the parent drug. Microbial growth inhibition bioassays with Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus were performed to determine the potential loss of antibacterial activity in isolated degradation byproducts. An increase of antibacterial activity in the DPs was observed compared to the parent compound.
Antonietta Siciliano; Marco Guida; Giovanni Libralato; Lorenzo Saviano; Giovanni Luongo; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. Amoxicillin in Water: Insights into Relative Reactivity, Byproduct Formation, and Toxicological Interactions during Chlorination. Applied Sciences 2021, 11, 1076 .
AMA StyleAntonietta Siciliano, Marco Guida, Giovanni Libralato, Lorenzo Saviano, Giovanni Luongo, Lucio Previtera, Giovanni Di Fabio, Armando Zarrelli. Amoxicillin in Water: Insights into Relative Reactivity, Byproduct Formation, and Toxicological Interactions during Chlorination. Applied Sciences. 2021; 11 (3):1076.
Chicago/Turabian StyleAntonietta Siciliano; Marco Guida; Giovanni Libralato; Lorenzo Saviano; Giovanni Luongo; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. 2021. "Amoxicillin in Water: Insights into Relative Reactivity, Byproduct Formation, and Toxicological Interactions during Chlorination." Applied Sciences 11, no. 3: 1076.
This study investigated sediment spatial and seasonal distribution of trace elements (TEs) (n = 16) and human health effects along the Hooghly River Estuary (India). The index of geo-accumulation (Igeo), enrichment factor (EF), hazard quotient (HQ), modified hazard quotient (mHQ) and toxic risk unit (TRI) were calculated to estimate sediment pollution level, while hazard index (HI) and lifetime cancer risk (LCR) were used to assess TEs enrichment vs. human health. The concentrations (µg/g dry weight) of TEs were: Cd (0.01–1.58), Cr (41.98–105.49), Cu (16.41–51.09), Ni (28.37–63.90), Fe (22075–47919), Mn (423–630), Co (11.43–23.11), Zn (48.82–105.81), V (63.92–138.92), Pb (25.01–43.27) and Ti (0.18–3.50); As (2.92–16.26), B (59.34–98.78), Si (11.52–98.78); Be (1.71–4.81), Ba (95.23–293.72). From Igeo and EF, Cd was the major contaminant, while Ni presented moderate/high contamination (HQ and TRI). Children were more exposed to carcinogenic and non-carcinogenic risks compared to adults. For non-carcinogenic substances, no significant risk was found to both children and adults (HIs < 1). The LCR for Cr (3.924 × 10−4 for children) and As (1.379 × 10−4 for children) was higher than the threshold limit value (TLV, 10−4 and 10−6) indicating significant carcinogenic risks to be managed.
Priyanka Mondal; Giusy Lofrano; Maurizio Carotenuto; Marco Guida; Marco Trifuoggi; Giovanni Libralato; Santosh Sarkar. Health Risk and Geochemical Assessment of Trace Elements in Surface Sediment along the Hooghly (Ganges) River Estuary (India). Water 2021, 13, 110 .
AMA StylePriyanka Mondal, Giusy Lofrano, Maurizio Carotenuto, Marco Guida, Marco Trifuoggi, Giovanni Libralato, Santosh Sarkar. Health Risk and Geochemical Assessment of Trace Elements in Surface Sediment along the Hooghly (Ganges) River Estuary (India). Water. 2021; 13 (2):110.
Chicago/Turabian StylePriyanka Mondal; Giusy Lofrano; Maurizio Carotenuto; Marco Guida; Marco Trifuoggi; Giovanni Libralato; Santosh Sarkar. 2021. "Health Risk and Geochemical Assessment of Trace Elements in Surface Sediment along the Hooghly (Ganges) River Estuary (India)." Water 13, no. 2: 110.
In recent years, many studies have highlighted the consistent finding of amoxicillin in waters destined for wastewater treatment plants, in addition to superficial waters of rivers and lakes in both Europe and North America. In this paper, the amoxicillin degradation pathway was investigated by simulating the chlorination process normally used in a wastewater treatment plant to reduce similar emerging pollutants at three different pH values. The structures of 16 isolated degradation byproducts (DPs), one of which was isolated for the first time, were separated on a C-18 column via a gradient HPLC method. Then, combining mass spectrometry (MALDI-MS/TOF) and nuclear magnetic resonance, we compared commercial standards and justified a proposed formation mechanism beginning from the parent drug. Microbial growth inhibition bioassays with Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus were performed to determine the potential loss of antibacterial activity in isolated degradation byproducts. An increase of antibacterial activity in the DPs was observed compared to the parent compound.
Antonietta Siciliano; Marco Guida; Giovanni Libralato; Lorenzo Saviano; Giovanni Luongo; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. Amoxicillin in Water: Insights Into Relative Reactivity, Byproduct Formation, and Toxicological Interactions During Chlorination. 2020, 1 .
AMA StyleAntonietta Siciliano, Marco Guida, Giovanni Libralato, Lorenzo Saviano, Giovanni Luongo, Lucio Previtera, Giovanni Di Fabio, Armando Zarrelli. Amoxicillin in Water: Insights Into Relative Reactivity, Byproduct Formation, and Toxicological Interactions During Chlorination. . 2020; ():1.
Chicago/Turabian StyleAntonietta Siciliano; Marco Guida; Giovanni Libralato; Lorenzo Saviano; Giovanni Luongo; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. 2020. "Amoxicillin in Water: Insights Into Relative Reactivity, Byproduct Formation, and Toxicological Interactions During Chlorination." , no. : 1.
Conventional fertilization practices in agroecosystems concern the supply of bioavailable nutrients, such as mineral fertilizers. A consolidated alternative to restoring the long-term fertility of agricultural soils is their amendment with organic fertilizers. Soil amendment with biowaste compost or sewage sludge represents a sustainable strategy to avoid the landfilling of organic matter derived from urban waste and sewage sludge. This study aims at validating the use of quality biowaste compost and sewage sludge from secondary sedimentation (alone or in combination with mineral fertilizers) in a Mediterranean agroecosystem and their effects on soil chemical and biological quality, with a view to verifying the maintenance of soil fertility and functionality. In particular, the dynamics of soil organic matter, pH, potentially toxic elements and microbial community functionality were assessed, in experimental mesocosms, during 6 months of incubation. The research showed that, while soil amendment with biowaste compost induces positive effects on soil organic matter and phosphorous concentrations, as well as on microbial community functionality, the amendment with the selected sewage sludge does not determine any benefit to the microbial community or any danger in relation to soil potentially toxic element concentrations and toxicity. The quantity of sewage sludge employed, chosen according to regional directives, was thus not enough to stimulate the edaphic microflora activity.
Enrica Picariello; Luca Pucci; Maurizio Carotenuto; Giovanni Libralato; Giusy Lofrano; Daniela Baldantoni. Compost and Sewage Sludge for the Improvement of Soil Chemical and Biological Quality of Mediterranean Agroecosystems. Sustainability 2020, 13, 26 .
AMA StyleEnrica Picariello, Luca Pucci, Maurizio Carotenuto, Giovanni Libralato, Giusy Lofrano, Daniela Baldantoni. Compost and Sewage Sludge for the Improvement of Soil Chemical and Biological Quality of Mediterranean Agroecosystems. Sustainability. 2020; 13 (1):26.
Chicago/Turabian StyleEnrica Picariello; Luca Pucci; Maurizio Carotenuto; Giovanni Libralato; Giusy Lofrano; Daniela Baldantoni. 2020. "Compost and Sewage Sludge for the Improvement of Soil Chemical and Biological Quality of Mediterranean Agroecosystems." Sustainability 13, no. 1: 26.
Diclofenac (DCF) is the most widely prescribed non-steroidal anti-inflammatory drug in the world and it has been detected in drinking and surface waters. In this paper, the effect of chlorination process on DCF in aqueous solutions was investigated and the structures of 14 isolated degradation by-products (DPs), of which nine are new, have been determined from combining mass spectrometry and nuclear magnetic resonance data and justified by a proposed mechanism of formation beginning from the parent drug. Some degradation by-products show only one phenyl, others are dimers or trimers of the parental compound, which has undergone oxidative decarboxylation of the side chain and/or chlorination of this or one or both aromatic rings. Ecotoxicological bioassays evidenced the following sensitivities D. magna < R. subcapitata < A. fischeri. The isolated DPs (DP1−8, except for DP9) exhibited effects ≥ 50 % in the exposed microalgae and crustaceans showing toxicities mainly ranked from slight to acute.
Giovanni Luongo; Marco Guida; Antonietta Siciliano; Giovanni Libralato; Lorenzo Saviano; Angela Amoresano; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. Oxidation of diclofenac in water by sodium hypochlorite: Identification of new degradation by-products and their ecotoxicological evaluation. Journal of Pharmaceutical and Biomedical Analysis 2020, 194, 113762 .
AMA StyleGiovanni Luongo, Marco Guida, Antonietta Siciliano, Giovanni Libralato, Lorenzo Saviano, Angela Amoresano, Lucio Previtera, Giovanni Di Fabio, Armando Zarrelli. Oxidation of diclofenac in water by sodium hypochlorite: Identification of new degradation by-products and their ecotoxicological evaluation. Journal of Pharmaceutical and Biomedical Analysis. 2020; 194 ():113762.
Chicago/Turabian StyleGiovanni Luongo; Marco Guida; Antonietta Siciliano; Giovanni Libralato; Lorenzo Saviano; Angela Amoresano; Lucio Previtera; Giovanni Di Fabio; Armando Zarrelli. 2020. "Oxidation of diclofenac in water by sodium hypochlorite: Identification of new degradation by-products and their ecotoxicological evaluation." Journal of Pharmaceutical and Biomedical Analysis 194, no. : 113762.
Contaminated sediment is a major issue for aquatic environments, but attention must be kept even during remediation activities that can negatively affect resident biota especially when applied in situ. For the first time, the species sensitivity distribution (SSD) approach was applied to amendments used for in situ sediment remediation considering 39 papers including both freshwater (F) and saltwater (S) effect data (i.e. n = 17 only F, n = 19 only S, and n = 3 both F and S). Toxicity data related to the application of activated carbon (AC), nano-Zero-Valent-Iron (nZVI), apatite (A), organoclay (OC) and zeolite (Z) were collected and analyzed. SSD curves were constructed by lognormal model providing comprehensive comparisons of the sensitivities of different species to the relative testing methods. Results indicated that Bacteria were the most sensitive group of testing organisms, while Crustaceans were the less sensitive. The hazardous concentration for 5% of the affected species (HC5) were derived to determine the concentration protecting 95% of the species. OC, A and Z presented both acute and chronic toxicity. The HC5 values in descending order are: AC (4.79 g/L) > nZVI (0.02 g/L) > OC, A and Z (1.77E-04 g/L). AC and nZVI can be considered safer than OC, A and Z in sediment remediation activities, even if in situ long-term effects remained still underexplored.
L. Albarano; G. Lofrano; M. Costantini; V. Zupo; F. Carraturo; M. Guida; G. Libralato. Comparison of in situ sediment remediation amendments: Risk perspectives from species sensitivity distribution. Environmental Pollution 2020, 272, 115995 .
AMA StyleL. Albarano, G. Lofrano, M. Costantini, V. Zupo, F. Carraturo, M. Guida, G. Libralato. Comparison of in situ sediment remediation amendments: Risk perspectives from species sensitivity distribution. Environmental Pollution. 2020; 272 ():115995.
Chicago/Turabian StyleL. Albarano; G. Lofrano; M. Costantini; V. Zupo; F. Carraturo; M. Guida; G. Libralato. 2020. "Comparison of in situ sediment remediation amendments: Risk perspectives from species sensitivity distribution." Environmental Pollution 272, no. : 115995.
The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (<0.1% to 38.3%), being positively correlated (p < 0.001) with regional atmospheric temperatures. Although most WWTPs removed large proportions of CTX-R coliforms, loads over 103 colony-forming units per mL were occasionally observed in final effluents. We demonstrate that CTX-R coliform monitoring is a feasible and affordable approach to assess wastewater antibiotic resistance status.
Roberto B.M. Marano; Telma Fernandes; Célia M. Manaia; Olga Nunes; Donald Morrison; Thomas U. Berendonk; Norbert Kreuzinger; Tanel Tenson; Gianluca Corno; Despo Fatta-Kassinos; Christophe Merlin; Edward Topp; Edouard Jurkevitch; Leonie Henn; Andrew Scott; Stefanie Heß; Katarzyna Slipko; Mailis Laht; Veljo Kisand; Andrea Di Cesare; Popi Karaolia; Stella G. Michael; Alice L. Petre; Roberto Rosal; Amy Pruden; Virginia Riquelme; Ana Agüera; Belen Esteban; Aneta Luczkiewicz; Agnieszka Kalinowska; Anne Leonard; William H. Gaze; Anthony A. Adegoke; Thor A. Stenstrom; Alfieri Pollice; Carlo Salerno; Carsten U. Schwermer; Pawel Krzeminski; Hélène Guilloteau; Erica Donner; Barbara Drigo; Giovanni Libralato; Marco Guida; Helmut Bürgmann; Karin Beck; Hemda Garelick; Marta Tacão; Isabel Henriques; Isabel Martínez-Alcalá; Jose M. Guillén-Navarro; Magdalena Popowska; Marta Piotrowska; Marcos Quintela-Baluja; Joshua T. Bunce; Maria I. Polo-López; Samira Nahim–Granados; Marie-Noëlle Pons; Milena Milakovic; Nikolina Udikovic-Kolic; Jérôme Ory; Traore Ousmane; Pilar Caballero; Antoni Oliver; Sara Rodriguez-Mozaz; Jose L. Balcazar; Thomas Jäger; Thomas Schwartz; Ying Yang; Shichun Zou; Yunho Lee; Younggun Yoon; Bastian Herzog; Heidrun Mayrhofer; Om Prakash; Yogesh Nimonkar; Ester Heath; Anna Baraniak; Joana Abreu-Silva; Manika Choudhury; Leonardo P. Munoz; Stela Krizanovic; Gianluca Brunetti; Ayella Maile-Moskowitz; Connor Brown; Eddie Cytryn. A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants. Environment International 2020, 144, 106035 .
AMA StyleRoberto B.M. Marano, Telma Fernandes, Célia M. Manaia, Olga Nunes, Donald Morrison, Thomas U. Berendonk, Norbert Kreuzinger, Tanel Tenson, Gianluca Corno, Despo Fatta-Kassinos, Christophe Merlin, Edward Topp, Edouard Jurkevitch, Leonie Henn, Andrew Scott, Stefanie Heß, Katarzyna Slipko, Mailis Laht, Veljo Kisand, Andrea Di Cesare, Popi Karaolia, Stella G. Michael, Alice L. Petre, Roberto Rosal, Amy Pruden, Virginia Riquelme, Ana Agüera, Belen Esteban, Aneta Luczkiewicz, Agnieszka Kalinowska, Anne Leonard, William H. Gaze, Anthony A. Adegoke, Thor A. Stenstrom, Alfieri Pollice, Carlo Salerno, Carsten U. Schwermer, Pawel Krzeminski, Hélène Guilloteau, Erica Donner, Barbara Drigo, Giovanni Libralato, Marco Guida, Helmut Bürgmann, Karin Beck, Hemda Garelick, Marta Tacão, Isabel Henriques, Isabel Martínez-Alcalá, Jose M. Guillén-Navarro, Magdalena Popowska, Marta Piotrowska, Marcos Quintela-Baluja, Joshua T. Bunce, Maria I. Polo-López, Samira Nahim–Granados, Marie-Noëlle Pons, Milena Milakovic, Nikolina Udikovic-Kolic, Jérôme Ory, Traore Ousmane, Pilar Caballero, Antoni Oliver, Sara Rodriguez-Mozaz, Jose L. Balcazar, Thomas Jäger, Thomas Schwartz, Ying Yang, Shichun Zou, Yunho Lee, Younggun Yoon, Bastian Herzog, Heidrun Mayrhofer, Om Prakash, Yogesh Nimonkar, Ester Heath, Anna Baraniak, Joana Abreu-Silva, Manika Choudhury, Leonardo P. Munoz, Stela Krizanovic, Gianluca Brunetti, Ayella Maile-Moskowitz, Connor Brown, Eddie Cytryn. A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants. Environment International. 2020; 144 ():106035.
Chicago/Turabian StyleRoberto B.M. Marano; Telma Fernandes; Célia M. Manaia; Olga Nunes; Donald Morrison; Thomas U. Berendonk; Norbert Kreuzinger; Tanel Tenson; Gianluca Corno; Despo Fatta-Kassinos; Christophe Merlin; Edward Topp; Edouard Jurkevitch; Leonie Henn; Andrew Scott; Stefanie Heß; Katarzyna Slipko; Mailis Laht; Veljo Kisand; Andrea Di Cesare; Popi Karaolia; Stella G. Michael; Alice L. Petre; Roberto Rosal; Amy Pruden; Virginia Riquelme; Ana Agüera; Belen Esteban; Aneta Luczkiewicz; Agnieszka Kalinowska; Anne Leonard; William H. Gaze; Anthony A. Adegoke; Thor A. Stenstrom; Alfieri Pollice; Carlo Salerno; Carsten U. Schwermer; Pawel Krzeminski; Hélène Guilloteau; Erica Donner; Barbara Drigo; Giovanni Libralato; Marco Guida; Helmut Bürgmann; Karin Beck; Hemda Garelick; Marta Tacão; Isabel Henriques; Isabel Martínez-Alcalá; Jose M. Guillén-Navarro; Magdalena Popowska; Marta Piotrowska; Marcos Quintela-Baluja; Joshua T. Bunce; Maria I. Polo-López; Samira Nahim–Granados; Marie-Noëlle Pons; Milena Milakovic; Nikolina Udikovic-Kolic; Jérôme Ory; Traore Ousmane; Pilar Caballero; Antoni Oliver; Sara Rodriguez-Mozaz; Jose L. Balcazar; Thomas Jäger; Thomas Schwartz; Ying Yang; Shichun Zou; Yunho Lee; Younggun Yoon; Bastian Herzog; Heidrun Mayrhofer; Om Prakash; Yogesh Nimonkar; Ester Heath; Anna Baraniak; Joana Abreu-Silva; Manika Choudhury; Leonardo P. Munoz; Stela Krizanovic; Gianluca Brunetti; Ayella Maile-Moskowitz; Connor Brown; Eddie Cytryn. 2020. "A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants." Environment International 144, no. : 106035.
To evaluate the hazard assessment of dredging and disposal of sediments, an in-depth chemical and toxicological characterization of sediments was carried out in Venice Lagoon. The bioaccessible and mobile concentration of trace elements in superficial and deep sediments (in the Holocene-Pleistocene limit) can deeply affect the quality of the aquatic ecosystem. Geospeciation and total concentration of trace elements in sediment cores were assessed and several toxicological tests were carried out. The indices of contamination (e.g., geochemical accumulation index, Igeo, pollution load index, PLI) and of speciation (individual contamination factor, ICF and global contamination factor, GCF) were evaluated. Factor Analysis (FA) was performed to explore possible significant correlations between toxicity data and sediment chemistry. Results underlined that the total concentration of trace elements cannot adequately assess the hazard, while bioavailability, mobility, and toxicity of trace elements allow a thorough evaluation of the environmental threats. The integration of results from chemical characterization ex ante and toxicity assays will provide for a better sustainable handling and management of sediments.
Fabiana Corami; Elisa Morabito; Andrea Gambaro; Paolo Cescon; Giovanni Libralato; Marco Picone; Anna Volpi Ghirardini; Carlo Barbante. Geospeciation, toxicological evaluation, and hazard assessment of trace elements in superficial and deep sediments. Environmental Science and Pollution Research 2020, 27, 15565 -15583.
AMA StyleFabiana Corami, Elisa Morabito, Andrea Gambaro, Paolo Cescon, Giovanni Libralato, Marco Picone, Anna Volpi Ghirardini, Carlo Barbante. Geospeciation, toxicological evaluation, and hazard assessment of trace elements in superficial and deep sediments. Environmental Science and Pollution Research. 2020; 27 (13):15565-15583.
Chicago/Turabian StyleFabiana Corami; Elisa Morabito; Andrea Gambaro; Paolo Cescon; Giovanni Libralato; Marco Picone; Anna Volpi Ghirardini; Carlo Barbante. 2020. "Geospeciation, toxicological evaluation, and hazard assessment of trace elements in superficial and deep sediments." Environmental Science and Pollution Research 27, no. 13: 15565-15583.
Microalgae are considered good bioindicators of marine environmental quality. Frequently, they are used to investigate the toxicity of sediment elutriates, but their sensitivity is disputed. This paper compared the sensitivity of Phaeodactylum tricornutum (diatom), Skeletonema costatum (diatom), and Dunaliella tertiolecta (green alga), analyzing 257 samples of elutriates (1:4 sediment: water ratio), considering growth inhibition (72 h) as the reference endpoint and sediment chemical (metals, metalloids and polyaromatic hydrocarbons) and grain size. Results of the toxicity tests showed that the microalgae sensitivity was not correlated. The integration of chemical data did not allow to discriminate toxicity effects but contributed to highlight that D. tertiolecta was the most sensitive microalgae (no cell wall) followed by P. tricornutum and S. costatum. Further analysis, including lines of evidence and weight of evidence approaches to calculate risk quotients of elutriate samples, confirmed these results.
A. Gallo; M. Guida; G. Armiento; A. Siciliano; N. Mormile; F. Carraturo; D. Pellegrini; L. Morroni; E. Tosti; M.I. Ferrante; M. Montresor; F. Molisso; M. Sacchi; R. Danovaro; G. Lofrano; G. Libralato. Species-specific sensitivity of three microalgae to sediment elutriates. Marine Environmental Research 2020, 156, 104901 .
AMA StyleA. Gallo, M. Guida, G. Armiento, A. Siciliano, N. Mormile, F. Carraturo, D. Pellegrini, L. Morroni, E. Tosti, M.I. Ferrante, M. Montresor, F. Molisso, M. Sacchi, R. Danovaro, G. Lofrano, G. Libralato. Species-specific sensitivity of three microalgae to sediment elutriates. Marine Environmental Research. 2020; 156 ():104901.
Chicago/Turabian StyleA. Gallo; M. Guida; G. Armiento; A. Siciliano; N. Mormile; F. Carraturo; D. Pellegrini; L. Morroni; E. Tosti; M.I. Ferrante; M. Montresor; F. Molisso; M. Sacchi; R. Danovaro; G. Lofrano; G. Libralato. 2020. "Species-specific sensitivity of three microalgae to sediment elutriates." Marine Environmental Research 156, no. : 104901.
Oxylipins are diatom-derived secondary metabolites, deriving from the oxidation of polyunsatured fatty acids that are released from cell membranes after cell damage or senescence of these single-celled algae. Previous results revealed harmful toxic effects of polyunsaturated aldehydes (PUAs) and hydroxyacids (HEPEs) on sea urchin Paracentrotus lividus embryonic development by testing individual compounds and mixtures of the same chemical group. Here, we investigated the combined effects of these compounds on sea urchin development at the morphological and molecular level for the first time. Our results demonstrated that oxylipin mixtures had stronger effects on sea urchin embryos compared with individual compounds, confirming that PUAs induce malformations and HEPEs cause developmental delay. This harmful effect was also confirmed by molecular analysis. Twelve new genes, involved in stress response and embryonic developmental processes, were isolated from the sea urchin P. lividus; these genes were found to be functionally interconnected with 11 genes already identified as a stress response of P. lividus embryos to single oxylipins. The expression levels of most of the analyzed genes targeted by oxylipin mixtures were involved in stress, skeletogenesis, development/differentiation, and detoxification processes. This work has important ecological implications, considering that PUAs and HEPEs represent the most abundant oxylipins in bloom-forming diatoms, opening new perspectives in understanding the molecular pathways activated by sea urchins exposed to diatom oxylipins.
Roberta Esposito; Nadia Ruocco; Luisa Albarano; Adrianna Ianora; Loredana Manfra; Giovanni Libralato; Maria Costantini. Combined Effects of Diatom-Derived Oxylipins on the Sea Urchin Paracentrotus lividus. International Journal of Molecular Sciences 2020, 21, 719 .
AMA StyleRoberta Esposito, Nadia Ruocco, Luisa Albarano, Adrianna Ianora, Loredana Manfra, Giovanni Libralato, Maria Costantini. Combined Effects of Diatom-Derived Oxylipins on the Sea Urchin Paracentrotus lividus. International Journal of Molecular Sciences. 2020; 21 (3):719.
Chicago/Turabian StyleRoberta Esposito; Nadia Ruocco; Luisa Albarano; Adrianna Ianora; Loredana Manfra; Giovanni Libralato; Maria Costantini. 2020. "Combined Effects of Diatom-Derived Oxylipins on the Sea Urchin Paracentrotus lividus." International Journal of Molecular Sciences 21, no. 3: 719.
This study investigated for the first time the effects of ZnO nanoparticle (NP) chronic exposure (28 days) on Tigriopus fulvus. Acute toxicity (48 h) of three Zn chemical forms was assessed as well including the following: (a) ZnO nanoparticles (NPs), (b) Zn2+ from ZnO NP suspension after centrifugation (supernatant) and (c) ZnSO4 H2O. Physical-chemical and electronic microscopies were used to characterize spiked exposure media. Results showed that the dissolution of ZnO NPs was significant, with a complete dissolution at lowest test concentrations, but nano- and micro-aggregates were always present. Acute test evidenced a significant higher toxicity of Zn2+ and ZnSO4 compared to ZnO NPs. The chronic exposure to ZnO NPs caused negative effects on the reproductive traits, i.e. brood duration, brood size and brood number at much lower concentrations (≥ 100 μg/L). The appearance of ovigerous females was delayed at higher concentrations of ZnO NPs, while the time required for offspring release and the percentage of non-viable eggs per female were significantly increased. ZnO NP subchronic exposure evidenced its ability to reduce T. fulvus individual reproductive fitness, suggesting that ZnO NPs use and release must be carefully monitored.
Ermelinda Prato; Isabella Parlapiano; Francesca Biandolino; Alice Rotini; Loredana Manfra; Maria Teresa Berducci; Chiara Maggi; Giovanni Libralato; Luigi Paduano; Federica Carraturo; Marco Trifuoggi; Maurizio Carotenuto; Luciana Migliore. Chronic sublethal effects of ZnO nanoparticles on Tigriopus fulvus (Copepoda, Harpacticoida). Environmental Science and Pollution Research 2019, 27, 30957 -30968.
AMA StyleErmelinda Prato, Isabella Parlapiano, Francesca Biandolino, Alice Rotini, Loredana Manfra, Maria Teresa Berducci, Chiara Maggi, Giovanni Libralato, Luigi Paduano, Federica Carraturo, Marco Trifuoggi, Maurizio Carotenuto, Luciana Migliore. Chronic sublethal effects of ZnO nanoparticles on Tigriopus fulvus (Copepoda, Harpacticoida). Environmental Science and Pollution Research. 2019; 27 (25):30957-30968.
Chicago/Turabian StyleErmelinda Prato; Isabella Parlapiano; Francesca Biandolino; Alice Rotini; Loredana Manfra; Maria Teresa Berducci; Chiara Maggi; Giovanni Libralato; Luigi Paduano; Federica Carraturo; Marco Trifuoggi; Maurizio Carotenuto; Luciana Migliore. 2019. "Chronic sublethal effects of ZnO nanoparticles on Tigriopus fulvus (Copepoda, Harpacticoida)." Environmental Science and Pollution Research 27, no. 25: 30957-30968.