Canopy characteristics are crucial for accurately and safely determining the pesticide quantity and volume of water used for spray applications in vineyards. The inevitably high degree of intraplot variability makes it difficult to develop a global solution for the optimal volume application rate. Here, the design procedure of, and the results obtained from, a variable rate application (VRA) sprayer are presented. Prescription maps were generated after detailed canopy characterization, using a multispectral camera embedded on an unmanned aerial vehicle, throughout the entire growing season in Torrelavit (Barcelona) in four vineyard plots of Chardonnay (2.35 ha), Merlot (2.97 ha), and Cabernet Sauvignonn (4.67 ha). The maps were obtained by merging multispectral images with information provided by DOSAVIÑA^®, a decision support system, to determine the optimal volume rate. They were then uploaded to the VRA prototype, obtaining actual variable application maps after the application processes were complete. The prototype had an adequate spray distribution quality, with coverage values in the range of 20–40% and exhibited similar results in terms of biological efficacy on powdery mildew compared to conventional (and constant) application volumes. The VRA results demonstrated an accurate and reasonable pesticide distribution, with potential for reduced disease damage even in cases with reduced amounts of plant protection products and water.

Several fungicides, such as copper and organic products (synthetic or natural), are currently being used in vineyards to control downy mildew (Plasmopara viticola) resulting in soil, surface water, and groundwater pollution. This study aims to assess the effectiveness of using cover crops as an agricultural practice in vineyards to protect soil and groundwater pollution. For that purpose, we performed different soil column studies to quantify soil leaching of selected fungicides (copper, dimethomorph, oxathiapiprolin, zoxamide, acibenzolar-s-methyl, and laminarin) following a rainfall event after a conventional fungicide vineyard application. Two types of vineyard soils (loam and sandy-loam soil textures) and three ground covers (bare ground, monoculture cover, and polyculture cover) were assessed. These studies were completed with hydroponic assays to check the effectiveness of cover roots in the fungicide degradation. Mass balance results show that whereas 3 fungicides (Cu, zoxamide, and dimethomorph) were leached through sandy soil columns, only copper was leached from loam soil columns. The effect of cover crops was only significant for Cu and zoxamide when fungicides were applied 24 h before the rain event, reducing the fungicide leaching by 30%. Hydroponic studies showed that cover roots enhanced the kinetic rates of almost all tested fungicides by 5-467%, suggesting that they are relevant to improving the degradation of fungicides in the soil column. These results are relevant to drawing up recommendations on the use of cover crops to protect soil and groundwater pollution by fungicides.

Aspergillus carbonarius causes severe decays on berries in vineyards and is among the main fungal species responsible for grape contamination by ochratoxin A (OTA), which is the foremost mycotoxin produced by this fungus. The main goal of this study was to investigate at the transcriptome level the comparative profiles between two table grape varieties (Victoria and Fraoula, the white and red variety, respectively) after their inoculation with a virulent OTA-producing A. carbonarius strain. The two varieties revealed quite different transcriptomic signatures and the expression profiles of the differential expressed genes (DEGs) highlighted distinct and variety-specific responses during the infection period. The significant enrichment of pathways related to the modulation of transcriptional dynamics towards the activation of defence responses, the triggering of the metabolic shunt for the biosynthesis of secondary metabolites, mainly phenylpropanoids, and the upregulation of DEGs encoding phytoalexins, transcription factors, and genes involved in plant–pathogen interaction and immune signaling transduction was revealed in an early time point in Fraoula, whereas, in Victoria, any transcriptional reprogramming was observed after a delay. However, both varieties, to some extent, also showed common expression dynamics for specific DEG families, such as those encoding for laccases and stilbene synthases. Jasmonate (JA) may play a critical modulator role in the defence machinery as various JA-biosynthetic DEGs were upregulated. Along with the broader modulation of the transcriptome that was observed in white grape, expression profiles of specific A. carbonarius genes related to pathogenesis, fungal sporulation, and conidiation highlight the higher susceptibility of Victoria. Furthermore, the A. carbonarius transcriptional patterns directly associated with the regulation of the pathogen OTA-biosynthesis gene cluster were more highly induced in Victoria than in Fraoula. The latter was less contaminated by OTA and showed substantially lower sporulation. These findings contribute to uncovering the interplay beyond this plant–microbe interaction.