In this paper, we propose a combination of mean-shift-based tracking processes to establish migrating cell trajectories through in vitro phase-contrast video microscopy. After a recapitulation on how the mean-shift algorithm permits efficient object tracking we describe the proposed extension and apply it to the in vitro cell tracking problem. In this application, the cells are unmarked (i.e., no fluorescent probe is used) and are observed under classical phase-contrast microscopy. By introducing an adaptive combination of several kernels, we address several problems such as variations in size and shape of the tracked objects (e.g., those occurring in the case of cell membrane extensions), the presence of incomplete (or noncontrasted) object boundaries, partially overlapping objects and object splitting (in the case of cell divisions or mitoses). Comparing the tracking results automatically obtained to those generated manually by a human expert, we tested the stability of the different algorithm parameters and their effects on the tracking results. We also show how the method is resistant to a decrease in image resolution and accidental defocusing (which may occur during long experiments, e.g., dozens of hours). Finally, we applied our methodology on cancer cell tracking and showed that cytochalasin-D significantly inhibits cell motility.

Ballistocardiography (BCG) and seismocardiography (SCG) are non-invasive techniques used to record the micromovements induced by cardiovascular activity at the body’s center of mass and on the chest, respectively. Since their inception, their potential for evaluating cardiovascular health has been studied. However, both BCG and SCG are impacted by respiration, leading to a periodic modulation of these signals. As a result, data processing algorithms have been developed to exclude the respiratory signals, or recording protocols have been designed to limit the respiratory bias. Reviewing the present status of the literature reveals an increasing interest in applying these techniques to extract respiratory information, as well as cardiac information. The possibility of simultaneous monitoring of respiratory and cardiovascular signals via BCG or SCG enables the monitoring of vital signs during activities that require considerable mental concentration, in extreme environments, or during sleep, where data acquisition must occur without introducing recording bias due to irritating monitoring equipment. This work aims to provide a theoretical and practical overview of cardiopulmonary interaction based on BCG and SCG signals. It covers the recent improvements in extracting respiratory signals, computing markers of the cardiorespiratory interaction with practical applications, and investigating sleep breathing disorders, as well as a comparison of different sensors used for these applications. According to the results of this review, recent studies have mainly concentrated on a few domains, especially sleep studies and heart rate variability computation. Even in those instances, the study population is not always large or diversified. Furthermore, BCG and SCG are prone to movement artifacts and are relatively subject dependent. However, the growing tendency toward artificial intelligence may help achieve a more accurate and efficient diagnosis. These encouraging results bring hope that, in the near future, such compact, lightweight BCG and SCG devices will offer a good proxy for the gold standard methods for assessing cardiorespiratory function, with the added benefit of being able to perform measurements in real-world situations, outside of the clinic, and thus decrease costs and time.

This paper addresses the issue of the satellite image processing using GRASS GIS in the mangrove forests of the Niger River Delta, southern Nigeria. The estuary of the Niger River Delta in the Gulf of Guinea is an essential hotspot of biodiversity on the western coast of Africa. At the same time, climate issues and anthropogenic factors affect vulnerable coastal ecosystems and result in the rapid decline of mangrove habitats. This motivates monitoring of the vegetation patterns using advanced cartographic methods and data analysis. As a response to this need, this study aimed to calculate and map several vegetation indices (VI) using scripts as advanced programming methods integrated in geospatial studies. The data include four Landsat 8-9 OLI/TIRS images covering the western segment of the Niger River Delta in the Bight of Benin for 2013, 2015, 2021, and 2022. The techniques included the ’i.vi’, ’i.landsat.toar’ and other modules of the GRASS GIS. Based on the GRASS GIS ’i.vi’ module, ten VI were computed and mapped for the western segment of the Niger River Delta estuary: Atmospherically Resistant Vegetation Index (ARVI), Green Atmospherically Resistant Vegetation Index (GARI), Green Vegetation Index (GVI), Difference Vegetation Index (DVI), Perpendicular Vegetation Index (PVI), Global Environmental Monitoring Index (GEMI), Normalized Difference Water Index (NDWI), Second Modified Soil Adjusted Vegetation Index (MSAVI2), Infrared Percentage Vegetation Index (IPVI), and Enhanced Vegetation Index (EVI). The results showed variations in the vegetation patterns in mangrove habitats situated in the Niger River Delta over the last decade as well as the increase in urban areas (Onitsha, Sapele, Warri and Benin City) and settlements in the Delta State due to urbanization. The advanced techniques of the GRASS GIS of satellite image processing and analysis enabled us to identify and visualize changes in vegetation patterns. The technical excellence of the GRASS GIS in image processing and analysis was demonstrated in the scripts used in this study.

In this paper, an image analysis framework is formulated for Landsat-8 Operational Land Imager and Thermal Infrared Sensor (OLI/TIRS) scenes using the R programming language. The libraries of R are shown to be effective in remote sensing data processing tasks, such as classification using k-means clustering and computing the Normalized Difference Vegetation Index (NDVI). The data are processed using an integration of the RStoolbox, terra, raster, rgdal and auxiliary packages of R. The proposed approach to image processing using R is designed to exploit the parameters of image bands as cues to detect land cover types and vegetation parameters corresponding to the spectral reflectance of the objects represented on the Earth’s surface. Our method is effective at processing the time series of the images taken at various periods to monitor the landscape dynamics in the middle part of the Congo River basin, Democratic Republic of the Congo (DRC). Whereas previous approaches primarily used Geographic Information System (GIS) software, we proposed to explicitly use the scripting methods for satellite image analysis by applying the extended functionality of R. The application of scripts for geospatial data is an effective and robust method compared with the traditional approaches due to its high automation and machine-based graphical processing. The algorithms of the R libraries are adjusted to spatial operations, such as projections and transformations, object topology, classification and map algebra. The data include Landsat-8 OLI-TIRS covering the three regions along the Congo river, Bumba, Basoko and Kisangani, for the years 2013, 2015 and 2022. We also validate the performance of graphical data handling for cartographic visualization using R libraries for visualising changes in land cover types by k-means clustering and calculation of the NDVI for vegetation analysis.

In this paper, we propose an advanced scripting approach using Python and R for satellite image processing and modelling terrain in Côte d’Ivoire, West Africa. Data include Landsat 9 OLI/TIRS C2 L1 and the SRTM digital elevation model (DEM). The EarthPy library of Python and ‘raster’ and ‘terra’ packages of R are used as tools for data processing. The methodology includes computing vegetation indices to derive information on vegetation coverage and terrain modelling. Four vegetation indices were computed and visualised using R: the Normalized Difference Vegetation Index (NDVI), Enhanced Vegetation Index 2 (EVI2), Soil-Adjusted Vegetation Index (SAVI) and Atmospherically Resistant Vegetation Index 2 (ARVI2). The SAVI index is demonstrated to be more suitable and better adjusted to the vegetation analysis, which is beneficial for agricultural monitoring in Côte d’Ivoire. The terrain analysis is performed using Python and includes slope, aspect, hillshade and relief modelling with changed parameters for the sun azimuth and angle. The vegetation pattern in Côte d’Ivoire is heterogeneous, which reflects the complexity of the terrain structure. Therefore, the terrain and vegetation data modelling is aimed at the analysis of the relationship between the regional topography and environmental setting in the study area. The upscaled mapping is performed as regional environmental analysis of the Yamoussoukro surroundings and local topographic modelling of the Kossou Lake. The algorithms of the data processing include image resampling, band composition, statistical analysis and map algebra used for calculation of the vegetation indices in Côte d’Ivoire. This study demonstrates the effective application of the advanced programming algorithms in Python and R for satellite image processing.