Backgrounds: Burn injuries present significant medical challenges due to their complexity in healing and potential for severe scarring. This study evaluates the efficacy of Manuka honey in accelerating burn wound healing compared to conventional antibiotic ointments. Methods: Using a porcine model resembling human skin, nine Landrace breed female pigs with standardized deep dermal burns were treated with either Manuka honey in alginate or a combination of antibiotic ointments. Wound healing was assessed through macroscopic evaluation, a histopathological analysis, and immunohistochemical staining over a 60-day period. Results: Our findings indicate that the Manuka honey treatment was associated with significantly increased collagen density in the treated wounds compared to the control group (p < 0.05). The immunohistochemical analysis revealed lower macrophage activity (Iba1 staining) and a reduction in Ki67 expression on days 10 and 17 in the Manuka honey group, suggesting a more rapid transition toward tissue remodeling. The quantitative analysis showed a trend toward delayed epithelialization and increased inflammation in the control group, while wounds treated with Manuka honey exhibited faster reepithelialization and improved epidermal regeneration. However, additional studies are required to further assess collagen fiber organization and overall dermal architecture. Conclusions: These findings support the potential of Manuka honey as a beneficial treatment for burn wound healing, with evidence of enhanced reepithelialization and collagen deposition. Further research, including clinical trials, is necessary to fully elucidate its role in clinical practice and optimize treatment protocols.

Burn injuries present significant medical challenges due to their complexity in healing and potential for severe scarring. This study evaluates the efficacy of Manuka honey in accelerating burn wound healing and minimizing scar formation compared to conven-tional antibiotic ointments. Using a porcine model resembling human skin, nine Landrace breed female pigs with standardized deep dermal burns were treated with either Manuka honey in alginate or a combination of antibiotic ointment.Wound healing was assessed through macroscopic evaluation, histopathological analysis, and immuno-histochemical staining over a 60-day period. Our findings reveal that Manuka honey significantly accelerates reepithelialization, enhances epidermal regeneration, and re-duces the time to complete wound closure. Histopathological evaluation showed superior dermal reconstruction with Manuka honey, increased collagen density, and horizontally oriented fibres, indicating better-quality scar tissue. Immunohistochemical analysis showed lower macrophage activity and higher cell proliferative activity (Ki67 staining) in the Manuka honey group, contributing to faster healing.The control group exhibited delayed epithelialization, higher inflammation, and less organized collagen structure, leading to poorer healing outcomes. This study highlights Manuka honey as a promising treatment for burns, offering accelerated healing and improved scar aesthetics. Integration into clinical practice could enhance patient recovery and reduce the burden of burn injuries.

Introduction. Phenylketonuria is an inborn disorder of metabolism, a rare, hereditary disease caused by deficiency of phenylalanine hydroxylase enzyme necessary for conversion of phenylalanine into tyrosine. The aim of this study is to determine the incidence of hyperphenylalaninemia and classical phenylketonuria in population of the Autonomous Province of Vojvodina. Material and Methods. We performed retrospective analysis at Medical Genetics Service, the Institute for Youth and Health Care of Vojvodina and examined the clinical material of the previous fourteen years, during the interval from 2003-2016. The analysis of the obtained results was carried out using descriptive statistics. Results. During fourteen years, from 01.01.2003 to 31.12.2016, 27 newborns with hyperphenylalaninemia were detected, and the incidence of hyperphenylalaninemia in the Autonomous Province of Vojvodina was 1: 9.525. Classical phenylketonuria was detected in 15 persons during indicated period, and the incidence was 1:17.143. Conclusion. Phenylketonuria is a hereditary disease whose adverse effects can be avoided, if it is recognized in time, and if recommended treatment measures are adequately applied, thereby improving the quality of life of persons affected by the disease as well as the whole family, that is facilitated by the introduction and implementation of neonatal screening.

Background/Objectives: Knee joint position sense (KJPS) assessment lacks standardized testing protocols, and it remains unclear how commonly used non-weight-bearing protocols—standing, prone, and sitting—and target angle influence reproduction error. This study aimed to compare KJPS error across these three protocols and three target knee-flexion angles (30°, 45°, 60°) in non-athletic adolescent females. Methods: In this within-subjects study, 30 non-athletic female participants aged 15–18 years (16.47 ± 1.04) performed an active angle-reproduction task with the tested limb non-weight-bearing, recorded using a VICON motion analysis system. Absolute Error (AE) and Constant Error (CE) were calculated, together with the between-participant dispersion of CE (SD-CE). Results: AE showed a significant main effect of protocol (F = 4.33, p = 0.018, ηp² = 0.130), confirmed by permutation testing, but no significant angle effect or interaction. Sitting produced lower AE than prone testing (mean difference = 2.98°, Holm-adjusted p = 0.023). CE was significantly positive in all nine conditions, confirming a systematic tendency to overshoot the target angle. SD-CE was lowest in sitting, intermediate in standing, and highest in prone. Conclusions: Testing protocol influenced KJPS accuracy and between-participant dispersion, whereas no target-angle effect was detected within the tested protocol configurations. The sitting protocol emerged as a promising candidate for further reliability and standardization research in this population.