The potential of black soldier fly larvae (BSFL) and microalgae (MA) in poultry diets has garnered increasing interest due to their high nutritional value and reduced environmental footprint. BSFL represent a sustainable alternative to conventional protein sources such as soybean meal, whereas MA contributes to improved egg quality, particularly through its enrichment with polyunsaturated fatty acids. This study assessed the effects of BSFL and MA inclusion on the growth performance and egg quality of Rhode Island Red (RIR) laying hens. Three diets were formulated: Diet A (10% BSFL), Diet B (10% BSFL + 2% MA), and Diet C (commercial control). The diets were formulated to meet the age-specific nutrient requirements of RIR hens, according to the National Research Council (NRC, 1994) guidelines. A total of 96 four-week-old chicks were randomly allocated to six pens (n = 16 per pen) and provided ad libitum access to feed and water throughout the trial. The results demonstrated that the inclusion of BSFL and MA significantly influenced the growth rate, onset of lay, and egg characteristics. Hens fed Diet B exhibited the highest average weekly body weight gain (0.034 ± 0.001 kg/week); initiated laying at 20 weeks of age, three weeks earlier than hens on Diets B and C; and produced significantly heavier eggs (51.208 ± 0.511 g). Enhanced eggshell quality and yolk pigmentation were also observed. In addition, Diet B enhanced the nutritional profile of the eggs, yielding a higher albumen protein content (76.546 ± 1.382%DM) and lower lipid concentrations (0.451 ± 0.128%DM). These findings underscore the potential of BSFL and MA as functional feed ingredients for improving poultry performance and egg quality in a sustainable production system.

Lippia graveolens is one of the most important aromatic species in Mexico due to antioxidant and antibiotic activities reported in its essential oil. The aim of this work was to assess the effect of irrigation with aquaculture wastewater and salicylic acid addition on the production of phenolic compounds in L. graveolens. L. graveolens plants (14) were irrigated with aquaculture wastewater and (14) using Steiner solution for 28 days; at the same time, salicylic acid was exogenously applied at 0.0 (control), 0.5 and 1.0 mM concentrations in both treatments at 5 and 19 experimental days. The total phenolic content was measured by Folin–Ciocalteu, the flavonoid content was determined by the aluminum chloride method, and the antioxidant capacity was measured by DPPH and FRAP assays. The results showed an increase in the total phenolic and flavonoid content in plants irrigated with aquaculture wastewater solution (17.25 ± 2.35 to 38.16 ± 4.47 mg eq GA·g⁻¹ W). The antioxidant capacity was higher in plants irrigated with Steiner solution (98.52 mg eq T·g⁻¹ W). In conclusion, L. graveolens irrigated with aquaculture wastewater leads to an increase in the total phenolic content and Steiner-solution antioxidant capacity in plants.