Additives in fish feeds are used worldwide to provide better productivity and improve fish’s health in facing disease outbreaks. This study aimed to identify the protective effect of taurine on the immune-related parameters and antioxidant enzyme activities of Seriola rivoliana juveniles after being challenged with LPS (lipopolysaccharide). Previously, the fish were submitted to a feeding trial for 60 days with feed enriched with different doses of external taurine (0, 1%, and 2%). Juveniles fed on different doses of taurine were injected with LPS (0%, LPS+T0%; 1%, LPS+T1%; 2%, LPS+T2%), and a control group was injected with saline solution (LPS-). The immune-related mRNA expression was evaluated, as were lysozyme enzyme activity and antioxidant enzyme activities (superoxide dismutase—SOD; catalase). Regarding immune mRNA expression, all the treatments had a peak of expression at 24 h post-LPS-injection, with a sharp decrease at 72 h post-injection, reaching similar mRNA expression as at 0 h post-injection. The results showed that the LPS+T2% treatment improved the expression of il1-β, tnf-α, and tlr-3 at 24 h post-LPS injection. Antioxidant and lysozyme activities were higher in both treatments with taurine when compared to the LPS+T0% and LPS- groups after 72 h post-LPS injection. These results suggest using 2% of exogenous external taurine can improve immunocompetency and counteract the oxidative stress caused by exposure to LPS in S. rivoliana juveniles.
Aquaculture practices expose fish to several factors that may generate stress, modifying the balance between the production of reactive oxygen species (ROS) and the activity of antioxidant defenses that induce cell damage. Alpha-tocopherol (VE) improves the antioxidant capacity against ROS production in fish. A 50-day trial with longfin yellowtail (Seriola rivoliana) juveniles was conducted to compare the dietary supplementation of 500 mg/kg of VE against a control diet without VE supplementation on growth, lymphoid tissue enzymatic activity, immune-system-related gene expression, and the histology of the liver and spleen. Growth, weight gain, specific growth rate, feed conversion rate, and survival did not show significant differences (p > 0.05) among treatments. Fish fed with an α-tocopherol-enriched diet showed a higher enzymatic activity of superoxide dismutase in the liver (p < 0.05) and a lower percentage of melanomacrophage coverage area in the lymphoid organs (p < 0.05). Overexpression was observed of MyD88 and il-10 in the spleen, and il-1b in the liver in fish fed 500 mg/kg of VE, as well as overexpression of Toll-like 3 in the head kidney, spleen, and liver in fish fed the control diet. Dietary supplementation with VE reduces the effects of oxidative stress and improves lymphoid tissue defense and immune-related gene expression in S. rivoliana.
25 May 2023 in Revista de Gestão Social e Ambiental
Objective: This study aims to analyze the impacts of communication management on project (administration) from the point of view of PMI certified members in Brazil. Theoretical framework: The literature review addressed the fundamentals of communication, determining factors for effective communication, human and organizational factors and communication management in projects. Method: As a methodology, an exploratory, descriptive, bibliographic and field study was used. For data collection, an online semi-structured questionnaire was made available to Brazilian members affiliated with PMI. Results and conclusion: The project manager's ability to manage communications is crucial to project performance. Effective communication management ensures that the right information is accurately delivered to stakeholders, regardless of the tool used. Research implications: The research makes a contribution, and it is still effective, the need for professionals specialized in social communication to work in project management. Originality/value: The results obtained in this study are original and relevant to communication management in project management. Objetivo: O presente estudo tem como objetivo analisar os impactos da gestão da comunicação no gerenciamento de projetos de acordo com a visão dos membros certificados pelo PMI no Brasil. Referencial teórico: A revisão bibliográfica abordou os fundamentos da comunicação, fatores determinantes para uma comunicação eficaz, fatores humanos e organizacionais e o gerenciamento das comunicações em projetos. Método: Como metodologia foi utilizado um estudo exploratório, descritivo, bibliográfico e de campo. Para o levantamento de dados foi disponibilizado um questionário on-line, semiestruturado, para membros brasileiros filiados ao PMI. Resultados e conclusão: A habilidade do gerente de projetos em administrar as comunicações é crucial no desempenho do projeto. A gestão da comunicação eficaz garante que as informações corretas sejam entregues com exatidão às partes interessadas, independente da ferramenta utilizada. Implicações da pesquisa: A pesquisa traz uma contribuição e efetiva a necessidade da atuação de profissionais especializados em comunicação social na gestão de projetos. Originalidade/valor: Os resultados obtidos no presente estudo são originais e relevantes para a gestão da comunicação no gerenciamento de projetos.
Homoeopathy is an alternative medical system proposed by Samuel Hahnemann in the eighteenth century. It uses highly diluted and agitated substances that derived from plants, minerals or animals, which have shown to be effective in human medicine, agronomy, veterinary, and as a novelty, in marine aquaculture. Aquacultural homoeopathy has developed rapidly in recent years, partially motivated by the misuse of powerful drugs (hormones, antibiotics, disinfectants) that when solving a problem generate undesirable side effects. In the last 10 years, scientific articles have been published on its application in freshwater fish native to Brazil, obtaining beneficial effects on growth, survival, hepatosomatic index, development of muscle fibres and lipid content in muscle. At Centro de Investigaciones Biológicas del Noroeste (CIBNOR, Mexico: www.cibnor.mx), we have studied the effects of homoeopathy to improve the culture of economically important marine species of molluscs, fish and shrimp. In this chapter, we show a selection of different research with preliminary or advanced results, related to the use of homoeopathy and its impact on zootechnic, biochemical, genomic and transcriptomic parameters in marine molluscs, fish and crustaceans. The results obtained suggest that homoeopathy is an eco-friendly alternative applicable in aquaculture industry to improve various productive and health aspects.
We describe five new isolates of two Gambierdiscus species from Bahía de La Paz in the southern Gulf of California. Batch cultures of Gambierdiscus were established for morphological characterization using light microscopy (LM) and scanning electron microscopy (SEM). Pigment and amino acid profiles were also analyzed using high-performance liquid chromatography (HPLC-UV and HPLC-DAD). Finally, toxicity (CTX-like and MTX-like activity) was evaluated using the Artemia salina assay (ARTOX), mouse assay (MBA), marine fish assay (MFA), and fluorescent receptor binding assay (fRBA). These strains were identified as Gambierdiscus cf. caribaeus and Gambierdiscus cf. carpenteri. Toxicity for CTX-like and MTX-like activity was confirmed in all evaluated clones. Seven pigments were detected, with chlorophyll a, pyridine, Chl2, and diadinoxanthin being particularly noteworthy. For the first time, a screening of the amino acid profile of Gambierdiscus from the Pacific Ocean was conducted, which showed 14 amino acids for all strains except histidine, which was only present in G. cf. caribeaus. We report the presence of Gambierdiscus and Fukuyoa species in the Mexican Pacific, where ciguatera fish poisoning (CFP) cases have occurred.