Leslie Velarde Apaza

Dr. Leslie Velarde Apaza

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Research Focus & Metrics

Academic Career Length

4Years (2023-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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31 July 2025 in Water
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ACAzucena Chávez Collantes
Azucena Chávez Collantes
DLDanny Jarlis Vásquez Lozano
Danny Jarlis Vásquez Lozano
LALeslie Velarde Apaza
Leslie Velarde Apaza
Juan Pablo CuevasJuan Pablo Cuevas
Juan Pablo Cuevas
Richard Andi SolórzanoRichard Andi Solórzano
Richard Andi Solórzano
RFRicardo Flores-Marquez
Ricardo Flores-Marquez
Water infiltration into soil is a key process in regulating the hydrological cycle and sustaining ecosystem services in high-Andean environments. However, limited information is available regarding its dynamics in these ecosystems. This study evaluated the influence of three types of vegetation cover and soil properties on water infiltration in a high-Andean environment. A double-ring infiltrometer, the Water Drop Penetration Time (WDPT, s) method, and laboratory physicochemical characterization were employed. Soils under forest cover exhibited significantly higher quasi-steady infiltration rates (is, 0.248 ± 0.028 cm·min−1) compared to grazing areas (0.051 ± 0.016 cm·min−1) and agricultural lands (0.032 ± 0.013 cm·min−1). Soil organic matter content was positively correlated with is. The modified Kostiakov infiltration model provided the best overall fit, while the Horton model better described infiltration rates approaching is. Sand and clay fractions, along with K+, Ca2+, and Mg2+, were particularly significant during the soil’s wet stages. In drier stages, increased Na+ concentrations and decreased silt content were associated with higher water repellency. Based on WDPT, agricultural soils exhibited persistent hydrophilic behavior even after drying (median [IQR] from 0.61 [0.38] s to 1.24 [0.46] s), whereas forest (from 2.84 [3.73] s to 3.53 [24.17] s) and grazing soils (from 4.37 [1.95] s to 19.83 [109.33] s) transitioned to weakly or moderately hydrophobic patterns. These findings demonstrate that native Andean forest soils exhibit a higher infiltration capacity than soils under anthropogenic management (agriculture and grazing), highlighting the need to conserve and restore native vegetation cover to strengthen water resilience and mitigate the impacts of land-use change.

25 July 2025 in Crops
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DLDanny Jarlis Vásquez Lozano
Danny Jarlis Vásquez Lozano
CSCledy Ureta Sierra
Cledy Ureta Sierra
JRJoseph Campos Ruiz
Joseph Campos Ruiz
HMHéctor Andrés Ramírez Maguiña
Héctor Andrés Ramírez Maguiña
ACAzucena Chávez Collantes
Azucena Chávez Collantes
AMAttilio Israel Cadenillas Martinez
Attilio Israel Cadenillas Martinez
Native bean genotypes (Phaseolus vulgaris L.) play a crucial role in ensuring food security in the Andean region. However, their cultivation faces challenges, such as low yields and a high dependence on nitrogen fertilizers. Addressing these issues requires the development of sustainable strategies to enhance productivity. This study evaluated the interaction between Rhizobium phaseoli inoculation and three levels of phosphorus (P) and potassium (K) fertilization on the growth, yield, and nutritional profile of the ‘Tiachos bayo’ native bean variety under Andean field conditions. Two R. phaseoli strains (UNC-1 and CIAT-2) were tested in combination with three levels of chemical fertilization (0%, 50%, and 100%) using a factorial design under field conditions. Parameters assessed included nodule number, plant height, phenology, yield, and proximal grain composition. Results indicated that inoculation and fertilization levels significantly influenced nodulation, phenological phases, and crop yield. The highest yield (2172 kg·ha−1) and nodule number (78) were observed with the combined treatment of R. phaseoli CIAT-2 strain with 100% fertilization. It was concluded that R. phaseoli inoculation, when integrated with appropriate fertilization, enhances the productivity of native beans.

7 September 2025 in Research on Crops
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Journal Article
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SVSandy Vilchez-Navarro
Sandy Vilchez-Navarro
AMArturo Morales-Pizarro
Arturo Morales-Pizarro
HRHenry Hoseph Morocho Romero
Henry Hoseph Morocho Romero
SCSebastian Casas-Niño
Sebastian Casas-Niño
GHGabriela Cárdenas Huamán
Gabriela Cárdenas Huamán
FLFlavio Lozano-Isla
Flavio Lozano-Isla
Purple maize native to the Peruvian Andes, is a key source of anthocyanins with notable antioxidant properties. In field cultivation, seed germination and early seedling development are critical stages influencing crop establishment and yield. This study evaluated the effects of three stimulants, namely seaweed extract, sulfur dioxide, and milk whey, on the germination and early development of creole and hybrid purple maize varieties under controlled laboratory and greenhouse conditions. Seeds of each variety were subjected to seven pre-sowing treatments in a completely randomised design with three replications. Germination indices (imbibition rate, germination percentage, speed, and index) were assessed, followed by evaluation of eleven biometric traits at 15 days after emergence. Seaweed extract significantly enhanced plant height, shoot dry weight, and leaf dimensions in both varieties, particularly at 1.5 L/200 L. Sulfur dioxide improved root biomass and length, while low-dose milk whey treatments favoured moderate root growth. Creole seeds showed higher vigour in early germination, while hybrids responded better to stimulant application during early development. These findings demonstrate that biostimulants, particularly seaweed extract, can effectively enhance early growth traits in purple maize, offering potential for improving seedling establishment in sustainable production systems.

23 December 2025 in Applied Microbiology
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LMLizbeth Mamani-Rojas
Lizbeth Mamani-Rojas
RSRaihil Rabindranath Rengifo Sanchez
Raihil Rabindranath Rengifo Sanchez
LALeslie Velarde Apaza
Leslie Velarde Apaza
MRMax Augusto Ramirez Rojas
Max Augusto Ramirez Rojas
HSHector Cántaro Segura
Hector Cántaro Segura
Potato (Solanum tuberosum L.) is a key staple crop in the Peruvian Andes, but its productivity is threatened by fungal pathogens such as Rhizoctonia solani and Alternaria alternata. In this study, 71 native bacterial strains (39 from phyllosphere and 32 from rhizosphere) were isolated from potato plants across five agroecological zones of Peru and characterized for their plant growth-promoting (PGPR) and antagonistic traits. Actinomycetes demonstrated broader enzymatic profiles, with 2ACPP4 and 2ACPP8 showing high proteolytic (68.4%, 63.4%), lipolytic (59.5%, 60.6%), chitinolytic (32.7%, 35.5%) and amylolytic activity (76.3%, 71.5%). Strain 5ACPP5 (Streptomyces decoyicus) produced 42.8% chitinase and solubilized both dicalcium (120.6%) and tricalcium phosphate (122.3%). The highest IAA production was recorded in Bacillus strain 2BPP8 (95.4 µg/mL), while 5ACPP6 was the highest among Actinomycetes (83.4 µg/mL). Siderophore production was highest in 5ACPP5 (412.4%) and 2ACPP4 (406.8%). In vitro antagonism assays showed that 5ACPP5 inhibited R. solani and A. alternata by 86.4% and 68.9%, respectively, while Bacillus strain BPP4 reached 51.0% inhibition against A. alternata. In greenhouse trials, strain 4BPP8 significantly increased fresh tuber weight (11.91 g), while 5ACPP5 enhanced root biomass and reduced stem canker severity. Molecular identification confirmed BPP4 as Bacillus halotolerans and 5ACPP5 as Streptomyces decoyicus. These strains represent promising candidates for the development of bioinoculants for sustainable potato cultivation in Andean systems.

Verified
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GCGabriela Cárdenas-Huamán
Gabriela Cárdenas-Huamán
HRHenry Hoseph Morocho Romero
Henry Hoseph Morocho Romero
SNSebastian Casas Niño
Sebastian Casas Niño
SVSandy Graciela Vilchez
Sandy Graciela Vilchez
LALeslie Velarde Apaza
Leslie Velarde Apaza
FLFlavio Lozano-Isla
Flavio Lozano-Isla
Potato (Solanum tuberosum L.) is the third most important food crop worldwide and a cornerstone of food security across the Andean region. However, its production is increasingly threatened by the potato psyllid Bactericera cockerelli (Šulc), the vector of Candidatus Liberibacter solanacearum, the causal agent of the purple-top complex associated with zebra chip disease, which severely reduces both tuber yield and quality. This study was conducted from September 2024 to February 2025 in the province of Huancabamba, Peru, to evaluate the efficacy of biological and chemical control agents against B. cockerelli under field conditions. A randomized complete block design was implemented with five treatments and four replicates, totaling 20 experimental units, each consisting of 20 potato plants (S. tuberosum L.), of which 10 plants were evaluated. Treatments included an untreated control (T0), a chemical control (thiamethoxam + lambda-cyhalothrin, abamectin, and imidacloprid) (T1), and three biological control agents: Beauveria bassiana CCB LE-265 (>1.5 × 1010 conidia g−1) (T2), Paecilomyces lilacinus strain 251 (1.0 × 1010 conidia g−1) (T3), and Metarhizium anisopliae (1.0 × 1010 conidia g−1) (T4). Foliar applications targeted eggs, nymphs, and adults of the psyllid. Results indicated that B. cockerelli mortality across developmental stages was lower under biological treatments compared with T1, which achieved the lowest probability of purple-top symptom expression (46%) and a zebra chip incidence of 60.60%. Among the biological agents, M. anisopliae (T4) reduced incidence to 56.60%, while P. lilacinus (T3) demonstrated consistent suppression of nymphal populations. In terms of yield, T1 achieved the highest tuber weight (198.86 g plant−1) and number of tubers (7.74 plant−1), followed by T3 (5.08) and T4 (4.24). Nevertheless, all treatments exhibited low yields and small tuber sizes, likely due to unfavorable environmental conditions and the presence of the invasive pest. Overall, chemical control was more effective than biological agents; however, the latter showed considerable potential for integration into sustainable pest management programs. Importantly, vector suppression alone does not guarantee the absence of purple-top complex symptoms or zebra chip disease in potato tubers.

1 January 2023 in Chilean journal of agricultural & animal sciences
Verified
Journal Article
Closed access
GSGuido Sarmiento-Sarmiento
Guido Sarmiento-Sarmiento
WRWilliam Rivera-Bejarano
William Rivera-Bejarano
Laydy Mitsu Mena ChaconLaydy Mitsu Mena Chacon
Laydy Mitsu Mena Chacon
RQRené Quispe-Castro
René Quispe-Castro
LALeslie Velarde Apaza
Leslie Velarde Apaza
LLLuis Miguel Lipa-Mamani
Luis Miguel Lipa-Mamani
La demanda mundial de hortalizas saludables está en constante crecimiento debido a cambios en las preferencias de los consumidores por alimentos sanos, frescos y nutritivos. En este contexto, se requiere investigar en nuevas técnicas para optimizar el rendimiento de hortalizas como el brócoli, en base al uso de fuentes de materia orgánica. El objetivo de la investigación fue evaluar el efecto de la utilización de vermicompost, acolchado orgánico y cobertura plástica en la producción sostenible de brócoli y en algunas propiedades del suelo. El estudio se realizó en plantas de brócoli cv. Legacy, en un terreno agrícola ubicado en la quebrada de Tucos del anexo La Tomilla, distrito Cayma, provincia y región Arequipa, Perú. El experimento fue establecido con un diseño de bloques completos al azar en arreglo factorial de parcelas dividas. El tipo de cobertura (acolchado orgánico, cobertura plástica, y sin cobertura) se estableció en las parcelas grandes, y las dosis de vermicompost (4 y 8 t ha-1) en las parcelas pequeñas; de la interacción de los factores, surgieron 6 tratamientos con tres repeticiones. Los resultados demostraron que la cobertura plástica junto a la incorporación de 8 t ha-1 de vermicompost mejoraron el rendimiento del cultivo de brócoli (16666,66 kg ha-1), con un 98% de la producción alcanzando calidad de primera y 2% de segunda. Además, este tratamiento mantuvo la materia orgánica (4,27%), pH (7,1), salinidad (1,22 dS m-1), y temperatura (13,3 oC) del suelo en valores aceptables para el cultivo. La mayor rentabilidad del cultivo de brócoli fue 96,8%, con la utilización de cobertura plástica y 4 t ha-1 de vermicompost.