As part of the research directed at establishing the composition of the main products present in Ozoile^®, a study of the ozonolysis of the oleic acid methyl ester as well as triolein was undertaken. Starting from oleic acid methyl ester, all six ozonides were isolated for the first time and fully characterized. Then, we used a protocol based on ozonolysis of triolein and +OIL followed by trans-esterification of the crude reaction mixtures, which led to the same six ozonides. Furthermore, to exclude the formation of any other oxygenated compounds, both during the ozonolysis process and afterward, the reactivity towards ³O₂ and ¹O₂ was explored. The ozonolysis of oleic acid methyl ester in a participating solvent (MeOH) was also investigated.

The synthesis of glycosides and modified nucleosides represents a wide research field in organic chemistry. The classical methodology is based on coupling reactions between a glycosyl donor and an acceptor. An alternative strategy for new C-nucleosides is used in this approach, which consists of modifying a pre-existent furyl aglycone. This approach is applied to obtain novel pyridazine C-nucleosides starting with 2- and 3-(ribofuranosyl)furans. It is based on singlet oxygen [4+2] cycloaddition followed by reduction and hydrazine cyclization under neutral conditions. The mild three-step one-pot procedure leads stereoselectively to novel pyridazine C-nucleosides of pharmacological interest. The use of acetyls as protecting groups provides an elegant direct route to a deprotected new pyridazine C-nucleoside.

Objectives: Neonatal infections, which continue to be a major cause of morbidity and mortality globally, can be avoided with proper umbilical cord care. There is still no widely recognized standard for cord care despite numerous antiseptic techniques, and the hunt for safe, natural, and efficient substitutes is becoming necessary. In this regard, OZOILE, a formulation based on stable ozonides with wound-healing and antibacterial qualities, may be a viable choice. The purpose of this study was to retrospectively assess the impact of topical OZOILE on the timing of umbilical cord detachment and the avoidance or reduction of infection-related problems in newborns. Methods: The study was conducted in the Complex Operational Unit of Neonatology and Neonatal Intensive Care of the “Annunziata” Hospital-Cosenza (Italy) and included healthy newborns with a gestational age from the 35th to the 41st weeks of gestation, born to mothers with age ranging from 18 to 40 years. Results: Daily application of OZOILE spray oil, directly to the umbilical cord 3 times a day was able the stump fell off in a short time from 5 to 7 days and without any complications. OZOILE reduces secondary alterations of falling umbilical stump. Conclusions: In conclusion, OZOILE intervention could be a good candidate for umbilical cord care.

Background: Inflammatory bowel disease (IBD) is a chronic inflammatory condition, with therapy-resistant patients undergoing surgery and a high risk of developing colorectal cancer. Novel therapeutic approaches have shown limited efficacy in IBD treatment, highlighting the need for safer and more personalized strategies. The potential of natural compounds to modulate inflammation suggests their use as a potential adjunct therapy for IBD patients. Methods: Intestinal epithelial cells organoids (IECOs) were derived from IBD and non-IBD tissues from IBD patients, and levels of inflammation markers and epithelial barrier permeability were assayed using qRT-PCR, WB, IF and leaking assays in the presence of Ozoile, an extra virgin olive oil enriched in ozonides. The Luciferase-based IBD-like organoid platform was generated for preliminary screening of anti-inflammatory drugs. Results: In this study, we showed that IBD-ECOs recapitulate tissue architecture and pathological state. We showed that Ozoile has anti-inflammatory and epithelial barrier modulatory effects and that the Luciferase IBD-like organoid model is sensitive to anti-inflammatory compounds. Conclusions: Using IECOs, the specific anti-inflammatory and regenerative properties of Ozoile were assessed. Notably, our study highlights the potential of an IBD-like organoid platform to use in high-throughput screenings for rapid selection of anti-inflammatory drugs.