Chinese hawthorn (Crataegus pinnatifida) offers both medicinal and nutritional benefits due to its bioactive compounds. However, the combined effects of environmental and anthropogenic factors on its geographic distribution and medicinal quality remain insufficiently explored. Species distribution modeling (Biomod2) was integrated with ArcGIS-based spatial analysis to systematically model suitable habitats for C. pinnatifida across varying environmental and anthropogenic conditions, while concurrently assessing the spatial distribution patterns of medicinally active compounds. Furthermore, multi-scenario climate simulations were utilized to predict the impacts of future climate change on the spatial distribution patterns of this species. Anthropogenic activities strongly influence C. pinnatifida habitat suitability, followed by BIO18 (Precipitation of Warmest Quarter) and BIO4 (Temperature Seasonality). Bioactive compound heterogeneity is driven by anthropogenic activities, AWC (Soil Available Water Content), BIO12 (Annual Precipitation), and BIO4. High-suitability/high-quality areas are concentrated in Shandong Province. Under SSP126 (low emissions), high-suitability areas remain stable (2050s–2090s) with northeastward centroid shifts; under SSP585 (high emissions), they contract with southeastward centroid migration. Shandong is the optimal long-term cultivation region across scenarios. These findings establish a critical basis for cultivating C. pinnatifida with enhanced medicinal properties, developing adaptive management strategies to strengthen conservation, and ensuring sustainable utilization amid global climate change.

Background: Cultivation environments impose distinct abiotic and biotic stresses that act as primary drivers reshaping the metabolic profile and microbiome assembly of medicinal plants. This study investigates the impact of simulative habitat versus arched greenhouse cultivation on the synthesis of bioactive ginsenosides and the associated root microbiome structure in Panax ginseng. Methods: A combined metabolomics and microbiomics approach was applied to compare ginsenoside accumulation and rhizosphere microbial community composition under the two cultivation modes. Results: Ginseng from simulative habitat cultivation exhibited significantly higher ginsenoside content, particularly ginsenoside Re, compared to arched greenhouse cultivation, with this advantage being more pronounced in long-term cultivation. Microbiome profiling revealed that specific taxa, including Bradyrhizobium, were strongly enriched in simulative habitats and positively correlated with enhanced ginsenoside accumulation, suggesting a microbiome-mediated mechanism for metabolic plasticity. In contrast, arched greenhouse cultivation was associated with a more complex microbial structure characterized by increased negative interactions, which may compromise metabolic quality. Conclusions: These findings, utilizing multi-omics correlations, provide a theoretical basis for optimizing Panax ginseng quality through ecological cultivation strategies that leverage stress-responsive microbe–metabolite interactions.

The rhizome of Atractylodes lancea (Thunb.) DC. is a traditional Chinese medicine used extensively owing to its antimicrobial properties. It is utilized to treat nyctalopia and problems related to the gastrointestinal tract. However, its yield is limited because of its endangered status, long growth period, and restricted reproductive ability. Ancillary approaches have not been established to ensure sustainable resource utilization by applying efficient plant regeneration technologies and producing bioactive metabolites via genome editing. This study reports the effects of explants, hormones, and culture conditions on embryogenic callus induction, plant regeneration, adventitious and hairy root cultivation, and essential oil production. Embryogenic calli were successfully induced in MS and 2.0 mg/L 2,4-D and 1.0 mg/L NAA and 1/2MS medium supplemented with 4.0 mg/L 6-BA and 0.4 mg/L NAA, which were optimal for callus differentiation. Maximum proliferation (12-fold) of cluster buds was observed with a select combination of hormones [NAA (0.2 mg/L) and 6-BA (2.0 mg/L)]. “Efficient plant regeneration and bioactive metabolite production” can provide technical support for the protection and sustainable utilization of A. lancea germplasm resources in terms of resource preservation and new variety breeding, natural product production, and industrial breeding of medicinal plants.

Background/Objective: Anoectochilus roxburghii, a high-value medicinal orchid, faces significant challenges in quality standardization during large-scale tissue culture due to a lack of understanding of the underlying molecular mechanisms. This study aimed to compare “Jianlan No.2” plantlets cultured under a conventional tissue culture system (CK) and a sugar-free tissue culture system (TD), to elucidate the phenotypic and molecular basis for quality improvement. Methods: A systematic comparison was conducted. Phenotypic traits of plantlets from both systems were measured. Integrated transcriptomic (RNA sequencing) and untargeted metabolomic analyses were employed to identify the molecular differences at the gene expression and metabolite accumulation levels. Results: TD-grown seedlings exhibited significantly superior growth characteristics, including greater plant height, higher rooting rate, and improved transplant survival. Transcriptomic analysis identified 416 differentially expressed genes (DEGs) (44 upregulated, 372 downregulated in TD), which were significantly enriched in pathways related to cell wall organization, apoplast, and photosynthesis. Sixteen key genes were pinpointed as closely associated with seedling growth and metabolic regulation. Metabolomic profiling revealed 502 differentially accumulated metabolites (DAMs), with significant perturbations primarily in phenylpropanoid biosynthesis and terpenoid metabolism. Conclusions: The sugar-free tissue culture system enhances A. roxburghii seedling quality by coordinately modulating photosynthetic capacity, carbon metabolism, and the biosynthesis of key secondary metabolites. These findings provide a crucial molecular foundation for optimizing tissue culture protocols and advancing the standardized, high-quality cultivation of this valuable medicinal plant.

The Qinghai–Tibet Plateau hosts the rare alpine Tibetan medicinal herb Corydalis hendersonii Hemsl. The wild resources of C. hendersonii are being increasingly threatened by climate warming and intensified human activity. We employed a biomod2 ensemble species distribution model, integrating climatic variables, light/radiation variables, soil‐property layers, topographic variables, and the Human Footprint Index with 75 spatially rarefied occurrence records, to predict the potential geographic distribution and shifts in habitat suitability under SSP126 and SSP585 (2050s, 2070s, and 2090s), climate dependence, human‐footprint effects, and ecological niche dynamics. 10 algorithms were calibrated with pseudo‐absences and repeated resampling, and the high‐performing models (ROC > 0.9; TSS > 0.8) were combined into a weighted ensemble; XGBoost yielded the highest single‐model accuracy. Ultraviolet radiation (UV‐B) was the dominant predictor of suitability, followed by elevation and key thermal‐contrast variables (BIO1, BIO4, and BIO7), indicating strong adaptation to high‐altitude extreme environments characterized by intense radiation, low mean temperature, and large temperature amplitudes. The current high‐suitability habitats are concentrated in Tibet, with limited patches in southern Xinjiang, southern Qinghai, western Sichuan, and northern Yunnan, reflecting a narrow alpine niche. Future projections diverge strongly between pathways: under SSP126, moderate–high suitability is maintained and slightly expanded, whereas under SSP585, high‐suitability areas contract, leading to an overall shift toward higher‐elevation refugia and a northwestward migration of the centroid. When the Human Footprint Index layer was included, high‐suitability areas consistently decreased and peripheral contraction became more pronounced, indicating that climate‐only projections overestimate realized habitat area. Climatic niche overlap remained high overall but declined and fluctuated more under SSP585, suggesting niche displacement under intense climate warming. The results provide a basis for conserving C. hendersonii by prioritizing core habitats and implementing targeted management under future climate change scenarios.