Chinese hawthorn (Crataegus pinnatifida) offers both medicinal and nutritional benefits due to its bioactive compounds. However, the combined effects of environmental and anthropogenic factors on its geographic distribution and medicinal quality remain insufficiently explored. Species distribution modeling (Biomod2) was integrated with ArcGIS-based spatial analysis to systematically model suitable habitats for C. pinnatifida across varying environmental and anthropogenic conditions, while concurrently assessing the spatial distribution patterns of medicinally active compounds. Furthermore, multi-scenario climate simulations were utilized to predict the impacts of future climate change on the spatial distribution patterns of this species. Anthropogenic activities strongly influence C. pinnatifida habitat suitability, followed by BIO18 (Precipitation of Warmest Quarter) and BIO4 (Temperature Seasonality). Bioactive compound heterogeneity is driven by anthropogenic activities, AWC (Soil Available Water Content), BIO12 (Annual Precipitation), and BIO4. High-suitability/high-quality areas are concentrated in Shandong Province. Under SSP126 (low emissions), high-suitability areas remain stable (2050s–2090s) with northeastward centroid shifts; under SSP585 (high emissions), they contract with southeastward centroid migration. Shandong is the optimal long-term cultivation region across scenarios. These findings establish a critical basis for cultivating C. pinnatifida with enhanced medicinal properties, developing adaptive management strategies to strengthen conservation, and ensuring sustainable utilization amid global climate change.

The rhizome of Atractylodes lancea (Thunb.) DC. is a traditional Chinese medicine used extensively owing to its antimicrobial properties. It is utilized to treat nyctalopia and problems related to the gastrointestinal tract. However, its yield is limited because of its endangered status, long growth period, and restricted reproductive ability. Ancillary approaches have not been established to ensure sustainable resource utilization by applying efficient plant regeneration technologies and producing bioactive metabolites via genome editing. This study reports the effects of explants, hormones, and culture conditions on embryogenic callus induction, plant regeneration, adventitious and hairy root cultivation, and essential oil production. Embryogenic calli were successfully induced in MS and 2.0 mg/L 2,4-D and 1.0 mg/L NAA and 1/2MS medium supplemented with 4.0 mg/L 6-BA and 0.4 mg/L NAA, which were optimal for callus differentiation. Maximum proliferation (12-fold) of cluster buds was observed with a select combination of hormones [NAA (0.2 mg/L) and 6-BA (2.0 mg/L)]. “Efficient plant regeneration and bioactive metabolite production” can provide technical support for the protection and sustainable utilization of A. lancea germplasm resources in terms of resource preservation and new variety breeding, natural product production, and industrial breeding of medicinal plants.

Background/Objective: Anoectochilus roxburghii, a high-value medicinal orchid, faces significant challenges in quality standardization during large-scale tissue culture due to a lack of understanding of the underlying molecular mechanisms. This study aimed to compare “Jianlan No.2” plantlets cultured under a conventional tissue culture system (CK) and a sugar-free tissue culture system (TD), to elucidate the phenotypic and molecular basis for quality improvement. Methods: A systematic comparison was conducted. Phenotypic traits of plantlets from both systems were measured. Integrated transcriptomic (RNA sequencing) and untargeted metabolomic analyses were employed to identify the molecular differences at the gene expression and metabolite accumulation levels. Results: TD-grown seedlings exhibited significantly superior growth characteristics, including greater plant height, higher rooting rate, and improved transplant survival. Transcriptomic analysis identified 416 differentially expressed genes (DEGs) (44 upregulated, 372 downregulated in TD), which were significantly enriched in pathways related to cell wall organization, apoplast, and photosynthesis. Sixteen key genes were pinpointed as closely associated with seedling growth and metabolic regulation. Metabolomic profiling revealed 502 differentially accumulated metabolites (DAMs), with significant perturbations primarily in phenylpropanoid biosynthesis and terpenoid metabolism. Conclusions: The sugar-free tissue culture system enhances A. roxburghii seedling quality by coordinately modulating photosynthetic capacity, carbon metabolism, and the biosynthesis of key secondary metabolites. These findings provide a crucial molecular foundation for optimizing tissue culture protocols and advancing the standardized, high-quality cultivation of this valuable medicinal plant.

Goji berry is widely consumed worldwide and holds substantial market value, yet its cultivation faces significant threats from the goji berry psyllid (Bactericera gobica). Chemosensory-related genes play critical roles in regulating insect behaviors, which makes them key molecular targets for the development of environmentally friendly pest control strategies. However, chemosensory genes in B. gobica have not been previously identified or characterized. In this study, we sequenced transcriptomes from the antennae and body tissues of male and female B. gobica and annotated genes associated with chemosensory functions. We identified 15 odorant-binding proteins (OBPs), 18 chemosensory proteins (CSPs), 3 sensory neuron membrane proteins (SNMPs), 26 odorant receptors (ORs), 8 gustatory receptors (GRs), and 32 ionotropic receptors (IRs). Transcriptome data and a quantitative real-time PCR confirmed the tissue-specific expression patterns of these genes, with several genes, including three BgobOBPs, eight BgobCSPs, one BgobOR, two BgobGRs, and two BgobIR, highly expressed in the antennae, suggesting their role in olfactory recognition. BgobGR1 was most highly expressed among GRs, indicating its important role in gustatory perception. We also identified gene BgobGR5 with differential expression patterns between females and males. Our study represents the first characterization of chemosensory genes in a Bactericera species.