This study sought to examine the effects of systemic ozone (O₃) treatment on the healing of skin wounds induced on the dorsal surface of Wistar rats. The skin wounds were created using a 10 mm round punch following the sagittal medial plane in 72 rats. Then, the animals were randomly assigned to four groups, each receiving the following treatments: group C, which did not undergo treatment with the O₃/O₂ mixture; group OZ0.3, administered the O₃/O₂ mixture at a dose of 0.3 mg/kg; group OZ0.7, given the O₃/O₂ mixture at a dose of 0.7 mg/kg; and group OZ1.0, provided with the O₃/O₂ mixture at a dose of 1.0 mg/kg. Six animals from each group were euthanized at 7, 14, and 21 days postoperatively. Clinical, histological, histometric, and immunohistochemical (IHC) analyses were accomplished. Data from clinical and histometric assessments revealed that OZ0.7 and OZ1.0 demonstrated more favorable healing, with greater wound contraction observed in the OZ1.0 group at 14 and 21 days. Histologically, the OZ1.0 group exhibited aspects consistent with an accelerated tissue repair process. IHC analysis revealed greater vascular endothelial growth factor (VEGF) immunostaining in the OZ0.7 (7 days) and OZ1.0 (7 and 14 days) groups compared to the C group. Expression of transforming growth factor beta‐1 was significantly increased in the OZ0.7 (14 days) and OZ1.0 (7 and 14 days) groups compared to the C group. In conclusion, our data suggest that systemic use of O₃ enhanced tissue repair in cutaneous wounds in a dose‐dependent manner, with concentrations of 1.0 mg/kg providing the most beneficial effects. Furthermore, the results of this study implicate the use of O₃ for the treatment of skin wounds aiming at improving the healing process over time. Our findings suggest the use of O₃ as a viable alternative to enhance wound healing and repair.

This study assessed the effectiveness of the local use of green propolis-loaded lipid nanoparticles (GPlnp) as an adjuvant therapy to scaling and root planing (SRP) to manage experimental periodontitis (EP) in ovariectomized rats treated with zoledronate. Ten weeks before the experiment, 48 female rats were ovariectomized. On day 0, a ligature was installed in the lower first molar to induce EP. From day 0 to day 42, half of the rats were treated with vehicle (VEH), while the other half were treated with 100μg/Kg of zoledronate (ZOL). On day 14, the rats were allocated into the following groups: VEH-NLT, VEH-SRP, VEH-SRP-GPlnp, ZOL-NLT, ZOL-SRP, and ZOL-SRP-GPlnp. VEH-NLT and ZOL-NLT received no local treatment. VEH-SRP and ZOL-SRP received SRP and irrigation with physiological saline solution. VEH-SRP-GPlnp and ZOL-SRP-GPlnp received SRP and irrigation with GPlnp. A single SRP session was carried out, and four irrigation sessions were conducted (on days 14, 16, 18, and 20). On day 42, all animals were euthanized. The hemimandibles were processed for histological, histometric (percentage of total bone tissue (PTBT) and non-vital bone tissue (PNVBT)) and immunohistochemical (TNFα, IL-1β, and TRAP) analysis. VEH-SRP-GPlnp showed better tissue repair, higher PTBT, and lower immunolabeling for TNFα and IL-1β compared to the groups treated with VEH. ZOL-SRP-GPlnp showed a favorable tissue repair, with lower PNVBT, less local inflammation, and lower immunolabeling for TNFα and IL-1β compared to the groups treated with ZOL. Irrigation with GPlnp proved to be effective as an adjuvant therapy to SRP in treating EP in ovariectomized rats treated with zoledronate.

As exodontias são procedimentos odontológicos realizados rotineiramente na prática clínica odontológica e em muitos casos encontramos um íntimo contato das raízes dos dentes superiores com o seio maxilar. Tal estrutura anatômica contribui para deixar o viscerocrânio mais leve, desempenhando um papel crucial no sistema respiratório, aquecendo o ar inspirado e contribuindo para a ressonância vocal. Por apresentar grande proximidade com os dentes superiores, diversas complicações patológicas e iatrogenias podem acontecer durante a realização de exodontias, necessitando de intervenções cirúrgicas para serem solucionadas. Dentre os procedimentos cirúrgicos, a técnica de Caldwell-Luc modificada, possibilita a remoção de corpos estranhos do seio maxilar, inspeção detalhada, facilidade de execução e grande eficácia. O objetivo do presente trabalho é relatar um caso clínico no qual foi realizada a remoção bem-sucedida de um remanescente de raiz residual do seio maxilar utilizando a técnica de Caldwell-Luc modificada, destacando a importância do procedimento na prática cirúrgica contemporânea. Conclui-se que a presença de corpos estranhos do seio maxilar deve ser imediatamente abordada, sendo o acesso cirúrgico Caldwell-Luc modificado uma ótima escolha para resolução destes casos, evitando o surgimento de quadros infecciosos locais e sistêmicas.

As Ligas Acadêmicas (L.A.) são grupos de estudantes que buscam aprofundar seus conhecimentos em temas específicos, atendendo às necessidades da comunidade, sob a orientação de um docente escolhido pelos discentes. Sem fins lucrativos, essas ligas promovem o ensino, a pesquisa e a extensão. A Liga Acadêmica de Anatomia Orofacial (LAAO) foi criada para integrar a anatomia com a harmonização orofacial (HOF) e a odontologia forense, áreas pouco exploradas na graduação. Este estudo busca identificar se a L.A. contribui positivamente para a formação acadêmica e pessoal dos discentes e beneficia toda a comunidade acadêmica. Foi realizada uma pesquisa exploratória, com abordagens quantitativa e qualitativa. A amostra, não probabilística, foi composta por alunos voluntários, membros internos da LAAO, do 2º ao 12º semestre do curso de Odontologia, exceto aqueles envolvidos na elaboração deste artigo. A coleta de dados ocorreu por meio de um questionário aplicado via "Google Forms". A análise dos resultados destaca a importância das ligas acadêmicas na complementação da formação, promovendo a integração entre teoria e prática. Elas oferecem benefícios tanto para os estudantes quanto para a comunidade acadêmica, além de aprofundar conhecimentos essenciais para cursos da área da saúde e promover um currículo diferenciado. O estudo confirma que as Ligas Acadêmicas são fundamentais para a formação dos graduandos, proporciona benefícios significativos para o desenvolvimento acadêmico e pessoal. Os alunos reconhecem seu valor e impacto positivo na comunidade acadêmica, aprimora o aprendizado e incentiva a integração.

This study evaluated the effectiveness and safety of mechanical debridement (MD) in treating experimental peri-implantitis (EPI) in rats with osseointegrated implants, specifically those treated with high-dose zoledronate. Senescent Wistar rats underwent the extraction of their upper incisor, followed by immediate implant placement. After 8 weeks, the implants were exposed, and a transmucosal component was placed. The animals were divided into four groups: Control (C), ZOL, ZOL-EPI, and ZOL-EPI-MD. In the 9th week, drug treatment commenced, consisting of the administration of 0.45 mL of a vehicle (for group C) or zoledronate (for groups ZOL, ZOL-EPI, and ZOL-EPI-MD) every 4 days over 10 weeks. After 5 weeks of drug treatment, a cotton bandage was placed around the implants to induce EPI in the ZOL-EPI and ZOL-EPI-MD groups. In the ZOL-EPI-MD group, the ligature was removed at week 16, and local treatment was performed using MD. Euthanasia was conducted at week 19. Histological sections were obtained and stained with hematoxylin–eosin for histopathological and histometric analyses, such as the percentage of total bone tissue (B.Ar/T.Ar) and the percentage of non-vital bone tissue (NVB.Ar/B.Ar). Immunohistochemical reactions were performed to detect TNFα, IL-1β, VEGF, OCN, and TRAP. In the peri-implant connective tissue, mild, intense, and moderate inflammatory infiltrates were observed in the ZOL, ZOL-EPI, and ZOL-EPI-MD groups, respectively. Immunolabeling for TNFα and IL-1β correlated with these histopathological findings. The ZOL and ZOL-EPI-MD groups showed lower immunolabeling for VEGF compared to the control group. There was a reduction in TRAP-positive cells and lower immunolabeling for OCN in the groups treated with zoledronate, with the ZOL-EPI-MD group displaying even lower levels of OCN compared to the ZOL group. While there was no significant difference in B.Ar/T.Ar across the groups, both the ZOL, ZOL-EPI, and ZOL-EPI-MD groups exhibited higher levels of NVB.Ar/B.Ar, with the ZOL-EPI-MD group showing the highest NVB.Ar/B.Ar compared to ZOL and the other groups. In conclusion, MD, as a standalone treatment, showed neither effectiveness nor safety in the management of EPI in rats that received high doses of zoledronate.