Due to its divergent chemical composition and good nutritional properties, pollen is not only important as a potential food supplement but also as a good substrate for the development of different microorganisms. Among such microorganisms, toxigenic fungi are extremely dangerous as they can synthesize mycotoxins as a part of their metabolic pathways. Furthermore, favorable conditions that enable the synthesis of mycotoxins (adequate temperature, relative humidity, pH, and a_w values) are found frequently during pollen collection and/or production process. Internationally, several different mycotoxins have been identified in pollen samples, with a noted predominance of aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol, and T-2 toxin. Mycotoxins are, generally speaking, extremely harmful for humans and other mammals. Current EU legislation contains guidelines on the permissible content of this group of compounds, but without information pertaining to the content of mycotoxins in pollen. Currently only aflatoxins have been researched and discussed in the literature in regard to proposed limits. Therefore, the aim of this review is to give information about the presence of different mycotoxins in pollen samples collected all around the world, to propose possible aflatoxin contamination pathways, and to emphasize the importance of a regular mycotoxicological analysis of pollen. Furthermore, a suggestion is made regarding the legal regulation of pollen as a food supplement and the proposed tolerable limits for other mycotoxins.

Total fungal count, incidence of fungi and aflatoxin B1 (AFB1) concentration were studied in 33 samples of bee pollen randomly collected from beekeepers in Serbia. The total number of fungi was determined by dilution method whereas AFB1 was detected using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The mycological estimation showed the presence of nine genera of fungi as followed: Acremonium, Alternaria, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Penicillium and Rhizopus, with total number ranging from 1 x 103 to 1 x 105 CFU g-1. The results have shown the predominance of the fungi from the genera Aspergillus and Alternaria. Among Aspergillus species it was observed that the most frequent species was A. flavus with incidence of 27.27 %. Mycotoxin AFB1 was detected as 100% positive in all samples (100%) with an average concentration of 8.61 ?g kg-1. The obtained results indicated that honey bee pollen must be strictly controlled during its manipulation in the harvesting and manufacturing. Therefore, the implementation of good manufacturing (beekeeping) practice to define procedures for honeybee products could be crucial to reduce the risk of possible contamination and provide natural and safety product without risk on the human health.

Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 µg kg⁻¹) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.

This review provides a comprehensive overview of studies that have investigated different types of disposable tableware. First, the authors used text mining with the bibliometric analysis tool VOSViewer to identify the leading research streams in disposable tableware research. A systematic literature search was then conducted in two databases (Scopus and Web of Science) using a series of appropriate keywords. The review followed PRISMA guidelines to improve the transparency and quality of reporting. A total of 816 articles were screened by title and abstract, leaving 78 reports for full-text search. A total of 40 articles were included in this systematic review and categorised into studies focusing on the environment, food safety, or research perspectives, matching with the clusters identified through text mining. The studies in the environmental cluster either conducted life cycle assessments or investigated the biodegradation of different tableware items. The studies in the food safety cluster investigated the presence of substances that pose a health risk due to their potential migration. Finally, the last cluster included studies that investigated the possibilities of using new materials to produce more sustainable tableware, and a group of them also carried out sensory evaluations as a measure of their quality aspect. Overall, the results show that the development of a new generation of sustainable cutlery requires a holistic approach that considers environmental, food safety, and quality aspects.

The aim of this study was to evaluate the occurrence of total aflatoxins (AFs) and type-B fumonisins (FBs) in 65 maize grain samples collected during harvest in 2018 (13 samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) from different locations in suburb of Belgrade (Republic of Serbia). The average levels of AFs and FBs in mycotoxin-positive samples were 5.43 and 2910 ?g kg-1 (2018), 5.28 and 2710 ?g kg-1 (2019), 2.35 and 10980 ?g kg-1 (2020), 6.81 and 4950 ?g kg-1 (2021) and 5.32 and 20310 ?g kg-1 (2022), respectively. In 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 27.78% (2022) of maize samples, the co-occurrence of AFs and FBs was established. The maximum limits of 10 ?g kg-1 for AFs in maize and 4000 ?g kg-1 for FBs in unprocessed maize prescribed by regulations of Serbia and the European Union were exceeded for AFs in 14.29% (2021) and 5.56% (2022) of maize samples and for FBs in 7.69% (2018), 66.67% (2020), 28.57% (2021) and 41.67% (2022) of maize samples. Multiple linear regression analyses showed a statistically significant influence of climate factors (air temperature, relative humidity and total rainfall) in July-September (2018-2022) on FBs levels. These results indicate the need for continuous monitoring of the health status of harvested maize grains and risk assessment of the potential presence of mycotoxins in the food chain to avoid adverse effects on human and animal health.

This article provides answers to the question of how Generation Z perceives various quality characteristics in connection with paper straws. The aim of this study was to analyze the advantages and disadvantages of paper straws from three perspectives: intrinsic quality characteristics, sensory perception, and physical characteristics. A total of 215 young adults and 65 students aged up to 27 years took part in the field study and sensory analysis, respectively. The field survey revealed that Serbian Gen Z prefers and expects paper straws do not contribute to the flavor of the drink, have a rounded and dull edge, and not change their shape when dipped into the drink. The sensory panel was formed by selecting students based on the results of several sensory screening tests conducted in the initial phase. Sensory testing for the difference showed that the impact of paper straws of different diameters on flavor was limited, although it was confirmed that artificial saliva could easily coat the surfaces and penetrate the layers of the paper straws, resulting in a significant loss of firmness and a greater potential to adhere to the lips. No effects were found on the sweetness, sourness, pungency, and overall flavor of the soft drink. Viscosity was the only attribute that was affected. The soft drink was perceived as more viscous when using the narrower straw than when using the larger diameter straws. Artificial saliva and water were the liquids that had the greatest influence on the loss of firmness of the soaked straws. After 20 min of exposure to most of the liquids tested, the paper straws were found to lose over 75% of their firmness. Paper straws are more sustainable and have the potential to completely replace plastic straws. However, their physical properties, especially firmness, need to be further improved, considering that they can be soaked in liquids for more than 20 min when consuming various beverages.