Oregano and tarragon are widely cultivated culinary herbs used for food seasoning, having familiar characteristic aromas appreciated by the wide public. The aim of this research was to characterize essential oils (EOs) from locally sourced organic oregano and tarragon (Cluj, Romania) and study their bioactivity potential. Results showed that oregano EO had a sesquiterpene dominant profile responsible for strong bands between 2800 and 3000 cm⁻¹ on the Fourier transform infrared spectroscopy (FT-IR) spectrum and a composition consistent with reports from similar climatic regions. The tarragon EO profile was defined by phenylpropanoids responsible for the strong sharp peaks between 1000 and 1600 cm⁻¹ on the FT-IR spectrum. In oregano EO, 22 compounds were identified with β-caryophyllene as a major constituent. In tarragon EO, 20 compounds were identified with eugenol as a major constituent. Oregano EO had a stronger antibacterial effect against both Gram-negative and Gram-positive bacterial strains, while tarragon EO had a slightly stronger cytotoxic effect on three types of cancer cell lines tested (skin melanoma, prostate carcinoma, and colorectal adenocarcinoma). It was concluded that, given the fact that a sufficient supply of high-quality plant material can be available for EO extraction, culinary herbs can become reliable candidates for many industries without the risk of discontinued supply. Therefore, research aiming to widen their potential applications is welcome and worth pursuing.

Basil is a culinary herb in high demand all year round, either fresh, dried, or frozen. Field basil crops are commercially predominant, while greenhouse crops can cover the demand for fresh basil during the off-season. The leaf aspect of basil has great importance for the food industry, and a great diversity of genotypes has been created. The aim of this research was to conduct a comparative characterization of 12 basil genotypes (green and purple leaf) that present interest for breeding programs from a national germplasm collection. The study examines the accumulation of photosynthetic pigments in basil cultivated in field versus greenhouse conditions as indicators of plant performance and herb quality from the perspective of ensuring fresh plant material all year round. The experimental median indicated that photosynthetic leaf pigments accumulated in higher concentrations in the field relative to the greenhouse conditions, in descending order: β-carotene, lutein, chlorophyll a, and chlorophyll b. The trend was not as consistent for chlorophyll b, since four out of twelve genotypes presented higher levels in the greenhouse than in the field, while the overall values were not much lower in the greenhouse than the field (16.82%). All genotypes accumulated much higher carotenoid contents in field conditions relative to greenhouse conditions (>200%) and could also provide better nutritional advantages given their demonstrated health benefits. The differences in photosynthetic leaf pigments have both nutritional (the carotenoids), shelf-life, and processing relevance (chlorophylls) and serve as quality markers.

Nitrogen is a key determinant of both yield and quality in cereal crops; however, its efficiency is strongly influenced by environmental conditions and genotype. This study evaluated the impact of different sowing densities and nitrogen fertilization regimes on grain quality indices in four triticale (×Triticosecale Wittmack) varieties—Negoiu, Utrifun, Zvelt, and Tulnic—using a split-plot arrangement of the 4 × 3 × 3 type, under the climatic conditions of northwestern Romania. The experiment, conducted over two contrasting growing seasons (2021–2023), employed a split-plot design testing three sowing densities (450, 550, and 650 seeds/m²) and three fertilization levels: basic soil nitrogen fertilization, soil + foliar N-P-K application, and soil + foliar + biostimulant. The results indicated that climatic variability had a predominant effect on grain quality, followed by the genetic characteristics of the varieties and their response to water stress. In the drought-affected 2021–2022 season, the Zvelt variety recorded the highest protein content (14.2%), significantly outperforming the control (13.3%). Supplementary foliar fertilization and the use of biostimulants under drought conditions did not improve quality; in some cases, they led to significant decreases in protein content (from 14.36% to 13.69%) and thousand-kernel weight (TKW). Under optimal precipitation conditions in the 2022–2023 season, supplementary fertilization significantly improved hectoliter weight and TKW (reaching 46.7 g compared to 44.2 g in the soil-only treatments). Higher sowing densities (650 seeds/m²) generally led to decreases in hectoliter weight and TKW in favorable years. These results suggest that nitrogen fertilization can improve triticale quality. In this study, high yields, both quantitatively and qualitatively, appear to be mainly influenced by varieties and climatic conditions, especially water availability during critical growth stages.

Optimization of nitrogen (N) management is critical for enhancing maize (Zea mays L.) productivity while maintaining soil health. The present study investigated the impact of split-application fertilization strategies on soil chemical properties and grain yield across three distinct soil types (calcaric fluvisol, luvic phaeozem, and stagnic phaeozem) in Mureș County, Romania, over three cropping seasons (2022–2024). Three fertilization variants were evaluated: the first treatment, designated V1, involved the application of 300 kg/ha NPK 20-20-0 + 300 kg/ha urea, the second treatment V2 utilized 300 kg/ha NPK 20-20-0 + 300 kg/ha NAC 27 N-calcium ammonium nitrate, and the third treatment V3 served as the baseline control, receiving (300 kg/ha NPK 20-20-0). Results indicated that significant differences were observed among the three experimental sites representing contrasting soil types for soil chemical properties and maize productivity. Calcaric fluvisol exhibited the highest production potential, attaining a mean yield of 11,702.78 kg/ha. The impact of N supplementation on soil N levels and maize yield was found to be significant. The variant receiving urea supplementation (V1) achieved the highest median yield of 9560 kg/ha in comparison to the 7420 kg/ha obtained in the control. A strong positive correlation was observed between N index and yield across all soil types (ρ = 0.93 to 0.97, p < 0.001). Fertilization significantly influenced soil pH, CaCO₃ content, nitrogen index, phosphorus availability, and maize yield, whereas humus content remained relatively stable among treatments. These findings indicate that a split-fertilization regime combining NPK with urea provides a favorable balance between productivity and cost-effectiveness and maize output in the Transylvanian Plateau.