Background and Objectives: This study aimed to histologically evaluate the effects of local melatonin application at different doses on bone fracture healing. Materials and Methods: Thirty rats were divided into three groups, with ten rats in each group. In the control group (n = 10), a fracture line was created in the tibial bones, and fracture osteosynthesis was performed without any additional procedure. In the local melatonin dose 1 (MLT D-1) group (n = 10), a fracture line was created in the tibial bones, and 1.2 mg of lyophilized powder melatonin was applied locally before fracture osteosynthesis. In the local melatonin dose 2 (MLT D-2) group (n = 10), a fracture line was created in the tibial bones, and 3 mg of lyophilized powder melatonin was applied locally before fracture osteosynthesis. After a 12-week healing period, all subjects were sacrificed, and tibial bones were collected for histomorphometric analysis. Results: The percentage of bone formation was significantly higher in the MLT D-1 and MLT D-2 groups than in the control group. There was no statistically significant difference between the MLT D-1 and MLT D-2 groups. Conclusions: In conclusion, the study demonstrated that local melatonin application supports bone fracture by increasing bone formation, although different doses of melatonin did not lead to significant variations in fracture healing.

Bu çalışmada Kocaeli ilinde kliniklere getirilen kedi ve köpeklerde görülen kulak hastalıklarının prevelansının belirlenmesi amaçlandı. Bu amaçla Kocaeli ilinde kliniğe getirilen 112 kedi ve köpeğin genel kulak muayenesi gerçekleştirilmiş ve otoskopla kulak kanalı ve kulak zarı incelenmiştir. Ayrıca radyografik görüntüleme yöntemleri (röntgen), mikrobiyolojik ve histopatolojik muayeneler yapılmıştır. Hastalıklar teşhis edildikten sonra kaydedilmiş ve kulak hastalıkları ile ilgili değerlendirmeler istatistiksel olarak değerlendirilmiştir. Araştırma sonucunda Kocaeli’nde kulak hastalıkları prevelansının (%12,9) olduğu; hayvanların temizlik, beslenme ve barınma koşullarının iyileştirilmesinin bu olguların azalmasında etkili olacağı, periyodik olarak klinik muayenelerinin yapılmasının bu hastalıkların tanısının erken konularak hızlı ve etkili bir tedavi sürecine olanak sağlayacağı belirlenmiştir.

The aim of this study was to histologically and histomorphometrically investigate the effect of locally applied bovine amniotic fluid (BAF) on osseointegration levels in implants. Adult female Sprague–Dawley rats weighing 300–350 g were used as subjects. The rats were divided into two groups: the sham–operated control group (n=10) and the local BAF group (n=10). Implant cavities were created in the tibias of all subjects under sterile saline cooling with rotating instruments. Local BAF was applied to all implant sockets before the implants were placed. Rats were sacrificed after a four–week osseointegration period. Histological staining was performed using hematoxylin and eosin staining to analyze the osseointegration. Examinations of the bone implant connection (BIC) and peri–implant bone formation (PBF) were performed using a light microscope and an image analyzer. As a result of the analysis, the mean BIC value was 40.3 ± 4.9 for the sham–operated control group and 45.2 ± 7.7 for the local BAF group. The mean PBF was 39.9 ± 6.3 for the sham control group and 40.5 ± 5.7 for the local BAF group. A statistically significant difference was found between the sham control group and the local BAF group for the BIC and PBF values (P>0.05; P: 0.11; P: 0.83). The application of local BAF to the implant socket did not have a clear positive effect on implant osseointegration. More studies are needed to clarify the association between local BAF and osseointegration.

En este estudio, el objetivo fue examinar la aplicación local de líquido amniótico bovino en la curación de fracturas óseas en ratas. Veinte ratas hembra Sprague Dawley incluidas en el estudio se dividieron en 2 grupos de 10. El grupo simulado (n=10): se crearon fracturas óseas en la tibia derecha de las ratas y se fijaron con alambre de Kirschner. Después de un período de recuperación de cuatro semanas, los sujetos fueron sacrificados. Grupo de líquido amniótico bovino local (n = 10): se crearon fracturas óseas en los huesos de la tibia derecha de las ratas y se aplicó líquido amniótico bovino local durante la fijación con alambre de Kirschner. Después de un período de recuperación de cuatro semanas, los sujetos fueron sacrificados. Se descalcificaron muestras de todos los sujetos, se tiñeron con hematoxilina y eosina y se analizó la formación de hueso nuevo y la fibrosis. Cuando los grupos fueron evaluados en términos de regeneración ósea nueva, se determinó que la regeneración ósea nueva en los sujetos tratados con líquido amniótico bovino local fue estadísticamente significativamente mayor que en el grupo simulado (P<0,05). Cuando los grupos fueron evaluados en términos de fibrosis, se encontró que el valor de fibrosis en el grupo simulado era estadísticamente significativamente mayor en comparación con el grupo de líquido amniótico bovino local (P<0,05). Se puede afirmar que la aplicación local de líquido amniótico bovino puede afectar positivamente la curación de las fracturas óseas.

En tr The aim of this study was to determine the predisposing effect of the race on the incidence of foot diseases and nail deformities observed in the cattle brought to the clinic and to inform the patient owners in this direction. The animal material of the study consisted of 422 cattle from various races and ages were formed submitted to Fırat University Animal Hospital between 2015 and 2016 due to foot diseases and nail deformations. The data obtained were processed in herd reading record forms and statistical data were generated and presented as tables. In general foot diseases such as digital dermatitis, interdigital phlegmon, interdigital hyperplasia, bruised sole, ulcus solea and white line disease were observed in the study. As for nail deformations, most of the cases were short claws, scissors nails, sharp nails, corkscrew claws, splay or thick claws.In the light of the data obtained, the owners were informed on the importance of animal race selection in order to prevent foot diseasesnail deformations and the loss of yields and to provide added value to the national economy. Yapılan çalışmada, kliniğe getirilen sığırlarda gözlemlenen ayak hastalıkları ve tırnak deformiteleri üzerine ırk faktörünün predispoze etkisini istatistiksel olarak ortaya koymak ve bu yönde hasta sahiplerinin bilgilendirilmesi amaçlanmıştır. Çalışmanın materyalini; 2015 ve 2016 yılları arasında ayak hastalıkları ve tırnak deformasyonları yönünden Fırat Üniversitesi Hayvan Hastanesi’ne getirilen değişik ırk ve yaştan toplam 422 adet sığır oluşturdu. Elde edilen veriler sürü gözlem kayıt formlarına işlenerek istatistiksel verileri oluşturulup tablolar halinde sunuldu. Araştırmada ayak hastalıkları olarak; digital dermatitis, interdigital flegmon, interdigital hiperplazi, ökçe eziği, taban ülseri ve beyaz çizgi hastalığı, tırnak deformasyonları olarak ise daha çok küt tırnak, makas tırnak, sivri tırnak tirbüşon tırnak, ayrık tırnak ile yayvan ve dolgun tırnak olguları gözlemlendi.. Sonuç olarak; elde edilen veriler doğrultusunda hasta sahiplerine ayak hastalıkları ve tırnak deformasyonlarınn önlenebilmesi ve verim kayıplarına engel olup ülke ekonomisine katma değer sağlamak için coğrafik şartlara uygun hayvan ırk seçiminin önemi vurgulandı.