Background and Objectives: This study aimed to histologically evaluate the effects of local melatonin application at different doses on bone fracture healing. Materials and Methods: Thirty rats were divided into three groups, with ten rats in each group. In the control group (n = 10), a fracture line was created in the tibial bones, and fracture osteosynthesis was performed without any additional procedure. In the local melatonin dose 1 (MLT D-1) group (n = 10), a fracture line was created in the tibial bones, and 1.2 mg of lyophilized powder melatonin was applied locally before fracture osteosynthesis. In the local melatonin dose 2 (MLT D-2) group (n = 10), a fracture line was created in the tibial bones, and 3 mg of lyophilized powder melatonin was applied locally before fracture osteosynthesis. After a 12-week healing period, all subjects were sacrificed, and tibial bones were collected for histomorphometric analysis. Results: The percentage of bone formation was significantly higher in the MLT D-1 and MLT D-2 groups than in the control group. There was no statistically significant difference between the MLT D-1 and MLT D-2 groups. Conclusions: In conclusion, the study demonstrated that local melatonin application supports bone fracture by increasing bone formation, although different doses of melatonin did not lead to significant variations in fracture healing.

In?this study, the effects of?Aloe vera gel and coconut oil on?wound healing were investigated and compared in?rats. Forty-two Wistar albino rats were used during the experiment, in?which they were operated on?under general anaesthesia to?crea...

Bu çalışmada Fırat Üniversitesi Hayvan Hastanesi'nde 2002-2013 yılları arasında farklı hayvan türlerinde göz hastalıklarının görülme sıklığı araştırıldı. Çalışmadaki elde edilen veriler, Fırat Üniversitesi Hayvan Hastanesi kayıt sisteminden temin edildi. Çalışmada en sık karşılaşılan göz problemlerinin keratitis (%34.17) ve konjunktivitis (%31.29) olduğu saptandı. Konjunktivitis olgularına en çok köpeklerde (%34.48) rastlandığı tespit edilirken; keratitis olgularına ise en çok sığırlarda (%51.57) rastlandığı tespit edildi. Sığırlardaki keratitis olgularının %10’unun Coriza Gangrenosa Bovis kaynaklı olduğu tespit edildi. Sonuç olarak, yapılan bu çalışma ile Elazığ bölgesinde en çok karşılaşılan göz hastalıkları tespit edilerek bölgedeki veteriner hekimlerin göz hastalıklarına yaklaşımına yardımcı olmak amaçlandı.

The aim of this study was to histologically and histomorphometrically investigate the effect of locally applied bovine amniotic fluid (BAF) on osseointegration levels in implants. Adult female Sprague–Dawley rats weighing 300–350 g were used as subjects. The rats were divided into two groups: the sham–operated control group (n=10) and the local BAF group (n=10). Implant cavities were created in the tibias of all subjects under sterile saline cooling with rotating instruments. Local BAF was applied to all implant sockets before the implants were placed. Rats were sacrificed after a four–week osseointegration period. Histological staining was performed using hematoxylin and eosin staining to analyze the osseointegration. Examinations of the bone implant connection (BIC) and peri–implant bone formation (PBF) were performed using a light microscope and an image analyzer. As a result of the analysis, the mean BIC value was 40.3 ± 4.9 for the sham–operated control group and 45.2 ± 7.7 for the local BAF group. The mean PBF was 39.9 ± 6.3 for the sham control group and 40.5 ± 5.7 for the local BAF group. A statistically significant difference was found between the sham control group and the local BAF group for the BIC and PBF values (P>0.05; P: 0.11; P: 0.83). The application of local BAF to the implant socket did not have a clear positive effect on implant osseointegration. More studies are needed to clarify the association between local BAF and osseointegration.

En este estudio, el objetivo fue examinar la aplicación local de líquido amniótico bovino en la curación de fracturas óseas en ratas. Veinte ratas hembra Sprague Dawley incluidas en el estudio se dividieron en 2 grupos de 10. El grupo simulado (n=10): se crearon fracturas óseas en la tibia derecha de las ratas y se fijaron con alambre de Kirschner. Después de un período de recuperación de cuatro semanas, los sujetos fueron sacrificados. Grupo de líquido amniótico bovino local (n = 10): se crearon fracturas óseas en los huesos de la tibia derecha de las ratas y se aplicó líquido amniótico bovino local durante la fijación con alambre de Kirschner. Después de un período de recuperación de cuatro semanas, los sujetos fueron sacrificados. Se descalcificaron muestras de todos los sujetos, se tiñeron con hematoxilina y eosina y se analizó la formación de hueso nuevo y la fibrosis. Cuando los grupos fueron evaluados en términos de regeneración ósea nueva, se determinó que la regeneración ósea nueva en los sujetos tratados con líquido amniótico bovino local fue estadísticamente significativamente mayor que en el grupo simulado (P<0,05). Cuando los grupos fueron evaluados en términos de fibrosis, se encontró que el valor de fibrosis en el grupo simulado era estadísticamente significativamente mayor en comparación con el grupo de líquido amniótico bovino local (P<0,05). Se puede afirmar que la aplicación local de líquido amniótico bovino puede afectar positivamente la curación de las fracturas óseas.

This study aimed to investigate the effect of local melatonin application on bone healing in critical size defects created in rat tibias. 30 Spraque Dawley rats selected for the experimental setup were divided into three groups, each with 10 rats. In the control– defect group (n=10), only defects were created in the tibia bones of the rats, while 1.2 mg and 3 mg melatonin were applied locally to the defect–melatonin dose 1 (n=10) and defect melatonin dose 2 (n=10) groups, respectively. At the end of the experiment the subjects in all the groups were euthanized after an 8-week healing period and defect healing was calculated with histological bone healing percentage. The Shapiro–Wilk test and the Kolmogorov–Smirnov test were used to evaluate the conformity of the data to a normal distribution. One–way ANOVA was used to determine whether there was a difference between the groups due to the normal distribution of the data, and Tukey’s honestly significant difference (HSD) test was used in pairwise comparisons to determine from which group the difference originated. The healing rate in the defect melatonin dose 2 group was calculated as 53.2 ± 7.38% and the healing rate in the control group was calculated as 44 ± 6.38%, which was a significant increase (P=0.008); however, when the difference between the defect melatonin dose 1 group (53 ± 7.1%) and the defect melatonin dose 2 group (53. 2± 7.38%) was evaluated statistically, no significant difference was found.The bone healing percentage in the groups where local melatonin was applied was found to be statistically significantly higher compared to the control group (P0.05). Within the limits of this study, it can be said that local melatonin application applied at two different doses had a positive effect on bone healing, however, no difference was observed between the applied local melatonin doses in terms of bone healing.