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Warionia saharae Benth. & Coss. (Asteraceae) is an endemic species of North Africa naturally grown in the southwest of the Algerian Sahara. In the present study, this species’ hydromethanolic leaf extract was investigated for its phenolic profile characterized by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS). Additionally, the chemical composition of W. saharae was analyzed by gas chromatography–mass spectrometry, and its antioxidant potential was assessed through five in vitro tests: DPPH● scavenging activity, ABTS●+ scavenging assay, galvinoxyl scavenging activity, ferric reducing power (FRP), and cupric reducing antioxidant capacity. The UHPLC-DAD-ESI/MS analysis allowed the detection and quantification of 22 compounds, with taxifolin as the dominant compound. The GC–MS analysis allowed the identification of 37 compounds, and the antioxidant activity data indicate that W. saharae extract has a very high capacity to capture radicals due to its richness in compounds with antioxidant capacity. The extract also showed potent α-glucosidase inhibition as well as a good anti-inflammatory activity. However, weak anti-α-amylase and anticholinesterase activities were recorded. Moreover, an in silico docking study was performed to highlight possible interactions between three significant compounds identified in W. saharae extract and α-glucosidase enzyme.
Habiba Rechek; Ammar Haouat; Kaouther Hamaidia; Hamza Allal; Tarek Boudiar; Diana C. G. A. Pinto; Susana M. Cardoso; Chawki Bensouici; Noureddine Soltani; Artur M. S. Silva. Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae. Molecules 2021, 26, 5257 .
AMA StyleHabiba Rechek, Ammar Haouat, Kaouther Hamaidia, Hamza Allal, Tarek Boudiar, Diana C. G. A. Pinto, Susana M. Cardoso, Chawki Bensouici, Noureddine Soltani, Artur M. S. Silva. Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae. Molecules. 2021; 26 (17):5257.
Chicago/Turabian StyleHabiba Rechek; Ammar Haouat; Kaouther Hamaidia; Hamza Allal; Tarek Boudiar; Diana C. G. A. Pinto; Susana M. Cardoso; Chawki Bensouici; Noureddine Soltani; Artur M. S. Silva. 2021. "Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae." Molecules 26, no. 17: 5257.
Iron (Fe) deficiency causes great disturbances to plant growth, productivity and metabolism. This study investigated the effect of bicarbonate-induced Fe deficiency on Foeniculum vulgare (Mill) growth, nutrient uptake, the accumulation of secondary metabolites and the impact on bioactivities. When grown under indirect Fe deficiency conditions (+Fe +Bic), the plants decreased their total mass, an effect that was clearly evident in shoots (−28%). Instead, roots were the main organ affected regarding variations in the phenolic profile and their respective functionalities. Hydromethanolic extracts from bicarbonate-treated roots had a remarkable increase in the levels of phenolic compounds, both of flavonoids (isoquercetin and isorhamnetin) and phenolic acids (gallic acid, chlorogenic acid, syringic acid, ferulic acid, caffeic acid and trans-cinnamic acid), when compared to equivalent extracts from control plants. In addition, they exhibited higher scavenging abilities of DPPH•, NO•, RO2•, as well as inhibitory capacities towards the activity of lipoxygenase (LOX), xanthine oxidase (XO) and acetylcholinesterase (AChE). The overall results suggest that fennel species may modulate secondary metabolites metabolism to fight damages caused by iron deficiency.
Hanen Wasli; Nahida Jelali; Mariem Saada; Riadh Ksouri; Susana Cardoso. Insights on the Adaptation of Foeniculum vulgare Mill to Iron Deficiency. Applied Sciences 2021, 11, 7072 .
AMA StyleHanen Wasli, Nahida Jelali, Mariem Saada, Riadh Ksouri, Susana Cardoso. Insights on the Adaptation of Foeniculum vulgare Mill to Iron Deficiency. Applied Sciences. 2021; 11 (15):7072.
Chicago/Turabian StyleHanen Wasli; Nahida Jelali; Mariem Saada; Riadh Ksouri; Susana Cardoso. 2021. "Insights on the Adaptation of Foeniculum vulgare Mill to Iron Deficiency." Applied Sciences 11, no. 15: 7072.
Seaweeds are one of the largest producers of biomass in the marine environment and a source of multiple bioactive metabolites with valuable health benefits. Among these, phlorotannins have been widely recognized for their promising bioactive properties. The potential antitumor capacity of Fucus vesiculosus-derived phlorotannins remains, however, poorly explored, especially in gastrointestinal tract-related tumors. Therefore, this work aimed to evaluate the cytotoxic properties and possible mechanisms by which F. vesiculosus crude extract (CRD), phlorotannin-rich extract (EtOAc), and further phlorotannin-purified fractions (F1–F9) trigger cell death on different tumor cell lines of the gastrointestinal tract, using flow cytometry. The results indicate that F. vesiculosus samples exert specific cytotoxicity against tumor cell lines without affecting the viability of normal cells. Moreover, it was found that, among the nine different phlorotannin fractions tested, F5 was the most active against both Caco-2 colorectal and MKN-28 gastric cancer cells, inducing death via activation of both apoptosis and necrosis. The UHPLC-MS analysis of this fraction revealed, among others, the presence of a compound tentatively identified as eckstolonol and another as fucofurodiphlorethol, which could be mainly responsible for the promising cytotoxic effects observed in this sample. Overall, the results herein reported contribute to a better understanding of the mechanisms behind the antitumor properties of F. vesiculosus phlorotannin-rich extracts.
Marcelo Catarino; Iva Fernandes; Hélder Oliveira; Mylene Carrascal; Rita Ferreira; Artur Silva; Maria Cruz; Nuno Mateus; Susana Cardoso. Antitumor Activity of Fucus vesiculosus-Derived Phlorotannins through Activation of Apoptotic Signals in Gastric and Colorectal Tumor Cell Lines. International Journal of Molecular Sciences 2021, 22, 7604 .
AMA StyleMarcelo Catarino, Iva Fernandes, Hélder Oliveira, Mylene Carrascal, Rita Ferreira, Artur Silva, Maria Cruz, Nuno Mateus, Susana Cardoso. Antitumor Activity of Fucus vesiculosus-Derived Phlorotannins through Activation of Apoptotic Signals in Gastric and Colorectal Tumor Cell Lines. International Journal of Molecular Sciences. 2021; 22 (14):7604.
Chicago/Turabian StyleMarcelo Catarino; Iva Fernandes; Hélder Oliveira; Mylene Carrascal; Rita Ferreira; Artur Silva; Maria Cruz; Nuno Mateus; Susana Cardoso. 2021. "Antitumor Activity of Fucus vesiculosus-Derived Phlorotannins through Activation of Apoptotic Signals in Gastric and Colorectal Tumor Cell Lines." International Journal of Molecular Sciences 22, no. 14: 7604.
Ulva macroalgae species are recognized to be an underexploited source of key nutrients, including proteins, fibers and minerals. The present work evaluated the nutritional value protein, ash, mineral composition, fat, fatty acid profile and dietary fiber of the green macroalgae Ulva rigida produced in an open land-based integrated multi-trophic aquaculture (IMTA) system, over four seasons. Overall, protein and fat content of the farmed algae ranged between 7.6 and 25.8% DW and between 0.2 and 1.3% DW, respectively, reaching the highest levels during autumn/winter seasons. In turn, total dietary fiber and ashes showed a contrary seasonal tendency, reaching maximum levels in spring (40.9–58.3% DW and 25.5–38.8% DW, respectively). Notably, the latter were particularly characterized by their richness in potassium, magnesium and iron, and a sodium/potassium ratio bellow 1.7. Variable cultivation conditions of stocking density (Sd) and flow rate (Fr) were also tested, allowing to confirm that manipulation of cultivation conditions in an IMTA system may help to improve the nutritional value of this macroalga and to boost its market value through its use as a functional food ingredient.
Ana Queirós; Ana Circuncisão; Eduarda Pereira; Mónica Válega; Maria Abreu; Artur Silva; Susana Cardoso. Valuable Nutrients from Ulva rigida: Modulation by Seasonal and Cultivation Factors. Applied Sciences 2021, 11, 6137 .
AMA StyleAna Queirós, Ana Circuncisão, Eduarda Pereira, Mónica Válega, Maria Abreu, Artur Silva, Susana Cardoso. Valuable Nutrients from Ulva rigida: Modulation by Seasonal and Cultivation Factors. Applied Sciences. 2021; 11 (13):6137.
Chicago/Turabian StyleAna Queirós; Ana Circuncisão; Eduarda Pereira; Mónica Válega; Maria Abreu; Artur Silva; Susana Cardoso. 2021. "Valuable Nutrients from Ulva rigida: Modulation by Seasonal and Cultivation Factors." Applied Sciences 11, no. 13: 6137.
Recent studies indicate that plant polyphenols could be pointed as potential prebiotic candidates since they may interact with the gut microbiota, stimulating its growth and the production of metabolites. However, little is known about the fate of brown seaweeds’ phlorotannins during their passage throughout the gastrointestinal tract. This work aimed to evaluate the stability and bioaccessibility of Fucus vesiculosus phlorotannins after being submitted to a simulated digestive process, as well as their possible modulatory effects on gut microbiota and short-chain fatty acids production following a fermentation procedure using fecal inoculates to mimic the conditions of the large intestine. The stability of phlorotannins throughout the gastrointestinal tract was reduced, with a bioaccessibility index between 2 and 14%. Moreover, slight alterations in the growth of certain commensal bacteria were noticed, with Enterococcus spp. being the most enhanced group. Likewise, F. vesiculosus phlorotannins displayed striking capacity to enhance the levels of propionate and butyrate, which are two important short-chain fatty acids known for their role in intestinal homeostasis. In summary, this work provides valuable information regarding the behavior of F. vesiculosus phlorotannins along the gastrointestinal tract, presenting clear evidence that these compounds can positively contribute to the maintenance of a healthy gastrointestinal condition.
Marcelo Catarino; Catarina Marçal; Teresa Bonifácio-Lopes; Débora Campos; Nuno Mateus; Artur Silva; Maria Pintado; Susana Cardoso. Impact of Phlorotannin Extracts from Fucus vesiculosus on Human Gut Microbiota. Marine Drugs 2021, 19, 375 .
AMA StyleMarcelo Catarino, Catarina Marçal, Teresa Bonifácio-Lopes, Débora Campos, Nuno Mateus, Artur Silva, Maria Pintado, Susana Cardoso. Impact of Phlorotannin Extracts from Fucus vesiculosus on Human Gut Microbiota. Marine Drugs. 2021; 19 (7):375.
Chicago/Turabian StyleMarcelo Catarino; Catarina Marçal; Teresa Bonifácio-Lopes; Débora Campos; Nuno Mateus; Artur Silva; Maria Pintado; Susana Cardoso. 2021. "Impact of Phlorotannin Extracts from Fucus vesiculosus on Human Gut Microbiota." Marine Drugs 19, no. 7: 375.
According to the WHO, cancer was responsible for an estimated 9.6 million deaths in 2018, making it the second global leading cause of death. The main risk factors that lead to the development of this disease include poor behavioral and dietary habits, such as tobacco use, alcohol use and lack of fruit and vegetable intake, or physical inactivity. In turn, it is well known that polyphenols are deeply implicated with the lower rates of cancer in populations that consume high levels of plant derived foods. In this field, phlorotannins have been under the spotlight in recent years since they have shown exceptional bioactive properties, with great interest for application in food and pharmaceutical industries. Among their multiple bioactive properties, phlorotannins have revealed the capacity to interfere with several biochemical mechanisms that regulate oxidative stress, inflammation and tumorigenesis, which are central aspects in the pathogenesis of cancer. This versatility and ability to act either directly or indirectly at different stages and mechanisms of cancer growth make these compounds highly appealing for the development of new therapeutical strategies to address this world scourge. The present manuscript revises relevant studies focusing the effects of phlorotannins to counteract the oxidative stress–inflammation network, emphasizing their potential for application in cancer prevention and/or treatment.
Marcelo Catarino; Sónia Amarante; Nuno Mateus; Artur Silva; Susana Cardoso. Brown Algae Phlorotannins: A Marine Alternative to Break the Oxidative Stress, Inflammation and Cancer Network. Foods 2021, 10, 1478 .
AMA StyleMarcelo Catarino, Sónia Amarante, Nuno Mateus, Artur Silva, Susana Cardoso. Brown Algae Phlorotannins: A Marine Alternative to Break the Oxidative Stress, Inflammation and Cancer Network. Foods. 2021; 10 (7):1478.
Chicago/Turabian StyleMarcelo Catarino; Sónia Amarante; Nuno Mateus; Artur Silva; Susana Cardoso. 2021. "Brown Algae Phlorotannins: A Marine Alternative to Break the Oxidative Stress, Inflammation and Cancer Network." Foods 10, no. 7: 1478.
Oleuropein (OLE) and hydroxytyrosol (HT) are olive-derived phenols recognised as health-promoting agents with antioxidant, anti-inflammatory, cardioprotective, antifungal, antimicrobial, and antitumor activities, providing a wide range of applications as functional food ingredients. HT is Generally Recognised as Safe (GRAS) by the European Food Safety Authority (EFSA) and the Food and Drug Administration (FDA), whereas OLE is included in EFSA daily consumptions recommendations, albeit there is no official GRAS status for its pure form. Their application in food, however, may be hindered by challenges such as degradation caused by processing conditions and undesired sensorial properties (e.g., the astringency of OLE). Among the strategies to overcome such setbacks, the encapsulation in delivery systems and the covalent and non-covalent complexation are highlighted in this review. Additionally, the synthesis of OLE and HT derivatives are studied to improve their applicability. All in all, more research needs however to be carried out to investigate the impact of these approaches on the sensory properties of the final food product and its percussions at the gastrointestinal level, as well as on bioactivity. At last limitations of these approaches at a scale of the food industry must also be considered.
Mariana Monteiro; Andreia Silva; Daniela Resende; Susana Braga; Manuel Coimbra; Artur Silva; Susana Cardoso. Strategies to Broaden the Applications of Olive Biophenols Oleuropein and Hydroxytyrosol in Food Products. Antioxidants 2021, 10, 444 .
AMA StyleMariana Monteiro, Andreia Silva, Daniela Resende, Susana Braga, Manuel Coimbra, Artur Silva, Susana Cardoso. Strategies to Broaden the Applications of Olive Biophenols Oleuropein and Hydroxytyrosol in Food Products. Antioxidants. 2021; 10 (3):444.
Chicago/Turabian StyleMariana Monteiro; Andreia Silva; Daniela Resende; Susana Braga; Manuel Coimbra; Artur Silva; Susana Cardoso. 2021. "Strategies to Broaden the Applications of Olive Biophenols Oleuropein and Hydroxytyrosol in Food Products." Antioxidants 10, no. 3: 444.
Salvia species are known to have anti-inflammatory properties, and are traditionally used for healing purposes. Salvia verbenaca is an Algerian plant used for healing wounds and ulcers. This work aims to assess the acute and subacute safety of S. verbenaca and its possible anti-inflammatory activity as a mechanism contributing to its traditional applications. Lethal toxicity of S. verbenaca hydromethanolic extract was evaluated against Artemia salina larvae, while acute and subacute toxicity were orally tested on mice. The anti-inflammatory activity was screened ex vivo using membrane stabilization and in vivo using xylene induced ear edema as an acute inflammation model. The antiradical, reducing power and iron chelating activities of S. verbenaca were also investigated in vitro, and phenolic compounds were determined using UHPLC-DAD-ESI-MSn. Salvia verbenaca extract contained high amounts of phenolic compounds (206 μg GAE/mg extract). The in vitro antioxidant activity showed promising radical scavenging ability, iron chelating (IC50: 189 μg/mL), reducing power and strong anti-lipid-peroxidation effect (IC50: 111 μg/mL). The extract had potential cytotoxic effect against Artemia salina larvae (LC50: 30 μg/mL), but did not exhibit any acute/subacute toxicity effect on mice. Salvia verbenaca inhibited hypotonic and heat induced hemolysis and also reduced 50% of xylene induced ear edema at 600 mg/kg bw. Rosmarinic acid and caffeoylmalic acid were identified as the major compounds. Salvia verbenaca hydromethanolic extract was found to be safe at acute and subacute levels. Its in vitro/in vivo antioxidant activity, membrane stabilizing properties and anti-inflammatory activity may be an important aspect of its wound healing and anti-ulcer traditional use.
Nadjat Righi; Sabah Boumerfeg; Amirouche Deghima; Pedro A.R. Fernandes; Elisabete Coelho; Faiza Baali; Susana M. Cardoso; Manuel A. Coimbra; Abderrahmane Baghiani. Phenolic profile, safety assessment, and anti-inflammatory activity of Salvia verbenaca L. Journal of Ethnopharmacology 2021, 272, 113940 .
AMA StyleNadjat Righi, Sabah Boumerfeg, Amirouche Deghima, Pedro A.R. Fernandes, Elisabete Coelho, Faiza Baali, Susana M. Cardoso, Manuel A. Coimbra, Abderrahmane Baghiani. Phenolic profile, safety assessment, and anti-inflammatory activity of Salvia verbenaca L. Journal of Ethnopharmacology. 2021; 272 ():113940.
Chicago/Turabian StyleNadjat Righi; Sabah Boumerfeg; Amirouche Deghima; Pedro A.R. Fernandes; Elisabete Coelho; Faiza Baali; Susana M. Cardoso; Manuel A. Coimbra; Abderrahmane Baghiani. 2021. "Phenolic profile, safety assessment, and anti-inflammatory activity of Salvia verbenaca L." Journal of Ethnopharmacology 272, no. : 113940.
The present work evaluated the nutritional impact of macroalgae flours used as new ingredients in fermented sausages and the feasibility of using high-pressure processing (HPP) as a non-thermal pasteurization methodology to keep the quality attributes of the new food products. A commercial macroalgae mix was used in the formulation of new macroalgae-fortified meat frankfurter sausages (F-MFS), macroalgae-fortified vegetable frankfurter sausages (F-VFS) and in macroalgae-fortified traditional Portuguese sausage “chouriço” (F-TPS), overall incrementing the contents of Mg, K, Ca, Mn and Fe and decreasing the Na/K ratio. The application of HPP allowed extending the shelf-life of frankfurters by about 3-fold and improved the safety of “chouriço” along 180 days of storage, keeping its microbial load below the detection limit. The prevention of microbial growth in F-MFS and F-VFS was accompanied by pH stability of the products. In addition, no significant detriment on surface color and fatty acids was observed between pressurized and non-pressurized sausages, allowing consolidating the suitability of HPP in seaweed-fortified fermented sausages.
Catarina Marçal; Carlos Pinto; Artur Silva; Carla Monteiro; Jorge Saraiva; Susana Cardoso. Macroalgae-Fortified Sausages: Nutritional and Quality Aspects Influenced by Non-Thermal High-Pressure Processing. Foods 2021, 10, 209 .
AMA StyleCatarina Marçal, Carlos Pinto, Artur Silva, Carla Monteiro, Jorge Saraiva, Susana Cardoso. Macroalgae-Fortified Sausages: Nutritional and Quality Aspects Influenced by Non-Thermal High-Pressure Processing. Foods. 2021; 10 (2):209.
Chicago/Turabian StyleCatarina Marçal; Carlos Pinto; Artur Silva; Carla Monteiro; Jorge Saraiva; Susana Cardoso. 2021. "Macroalgae-Fortified Sausages: Nutritional and Quality Aspects Influenced by Non-Thermal High-Pressure Processing." Foods 10, no. 2: 209.
The present work aimed at the valorization of biomass derived compounds by their transformation into new added-value compounds with enhanced antioxidant properties. In this context, betulinic acid (BA) was decorated with polyphenolic fragments, and polyhydroxylated (E)-2-benzylidene-19,28-epoxyoleanane-3,28-diones 4a–d were obtained. For that, the synthetic strategy relied on base-promoted aldol condensation reactions of methyl betulonate, which was previously prepared from natural BA, with appropriate benzaldehydes, followed by cleavage of the methyl protecting groups with BBr3. It is noteworthy that the HBr release during the work-up of the cleavage reactions led to the rearrangement of the lupane-type skeleton of the expected betulonic acid derivatives into oleanane-type compounds 4a–d. The synthesized compounds 4a–d were designed to have specific substitution patterns at C-2 of the triterpene scaffold, allowing the establishment of a structure-activity relationship. The radical scavenging ability of 4a–d was evaluated using the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS•+) scavenging assays. In particular, derivative 4c, bearing a catechol unit, revealed to be the most efficient scavenger against both free radicals DPPH• and ABTS•+. Subsequently, we designed two analogues of the hit derivative 4c in order to achieve more potent antioxidant agents: (i) the first analogue carries an additional unsaturation in its lateral chain at C-2 (analogue 5) and (ii) in the second analogue, E-ring was kept in its open form (analogue 6). It was observed that the presence of an extended π-conjugated system at C-2 contributed to an increased scavenging effect, since analogue 5 was more active than 6, α-tocopherol, and 4c in the ABTS•+ assay.
Joana Sousa; Cristiana Gonçalves; Ricardo Ferreira; Susana Cardoso; Carmen Freire; Armando Silvestre; Artur Silva. Functionalization of Betulinic Acid with Polyphenolic Fragments for the Development of New Amphiphilic Antioxidants. Antioxidants 2021, 10, 148 .
AMA StyleJoana Sousa, Cristiana Gonçalves, Ricardo Ferreira, Susana Cardoso, Carmen Freire, Armando Silvestre, Artur Silva. Functionalization of Betulinic Acid with Polyphenolic Fragments for the Development of New Amphiphilic Antioxidants. Antioxidants. 2021; 10 (2):148.
Chicago/Turabian StyleJoana Sousa; Cristiana Gonçalves; Ricardo Ferreira; Susana Cardoso; Carmen Freire; Armando Silvestre; Artur Silva. 2021. "Functionalization of Betulinic Acid with Polyphenolic Fragments for the Development of New Amphiphilic Antioxidants." Antioxidants 10, no. 2: 148.
Microencapsulated resveratrol (RSV) is a pertinent ingredient in functional foods to be used in the prevention and management of cardiovascular diseases. Gamma-cyclodextrin (γ-CD) was evaluated for its RSV inclusion ability. Inclusion procedures comprised mixing equal concentration of an aqueous solution of γ-CD with an ethanol solution of RSV and freeze-drying to obtain a solid material. Solid-state characterization by vibrational spectroscopy, thermogravimetry, and powder X-ray diffraction (PXRD) confirmed the formation of the γ-CD·RSV complex in a ratio of 1:1. PXRD suggested that cyclodextrin molecules in the complex are stacked in infinite channels holding the RSV inside, with a wide inter-channel space where 14 water molecules are retained. Fresh lemon juices supplemented with 0.625 mg/mL of RSV in its free (RSV-juice) or complexed (γ-CD·RSV-juice) form were stored along 28 days under dark and room temperature or at 4 °C. Initially, the RSV level in γ-CD·RSV-juice was about nine times higher than in RSV-juice (43.1% and 4.8%, respectively), suggesting that the RSV complexation promoted its solubility in the lemon juice, a fact that was still noticed after 28 days of storage. Moreover, regardless the fact that the antioxidant capacity was similar among the juices, the loss of antiradical ABTS•+ capacity in γ-CD·RSV-juice was reduced compared to that of the RSV-juice. Overall, this study allowed concluding that γ-CD can serve as a carrier of RSV, promoting its solubility and eventually protecting its antioxidant stability in lemon juices for at least 28 days.
Andreia F. R. Silva; Mariana Monteiro; Daniela Resende; Susana S. Braga; Manuel A. Coimbra; Artur M. S. Silva; Susana M. Cardoso. Inclusion Complex of Resveratrol with γ-Cyclodextrin as a Functional Ingredient for Lemon Juices. Foods 2020, 10, 16 .
AMA StyleAndreia F. R. Silva, Mariana Monteiro, Daniela Resende, Susana S. Braga, Manuel A. Coimbra, Artur M. S. Silva, Susana M. Cardoso. Inclusion Complex of Resveratrol with γ-Cyclodextrin as a Functional Ingredient for Lemon Juices. Foods. 2020; 10 (1):16.
Chicago/Turabian StyleAndreia F. R. Silva; Mariana Monteiro; Daniela Resende; Susana S. Braga; Manuel A. Coimbra; Artur M. S. Silva; Susana M. Cardoso. 2020. "Inclusion Complex of Resveratrol with γ-Cyclodextrin as a Functional Ingredient for Lemon Juices." Foods 10, no. 1: 16.
Hydroxytyrosol (HT) is an amphipathic functional phenol found in the olive tree, both in its leaves and fruits, in free or bound forms, as well as in olive oil and by-products of olive oil manufacture. The European Food Safety Authority recommends regular consumption of HT due to its several beneficial effects on human health, which are closely associated to its antioxidant activity. These reasons make HT an excellent candidate for application as a functional ingredient in the design of novel food products. Patents already exist for methodologies of extraction, purification, and application of HT in supplements and food products. The present review discusses the impact of HT incorporation on food properties and its effects on consumers, based on relevant data related to the use of HT as a functional ingredient, both as a pure compound or in the form of HT-rich extracts, in various food products, namely in edible oils, beverages, bakery products, as well animal-based foods such as meat, fishery and dairy products.
Andreia F. R. Silva; Daniela Resende; Mariana Monteiro; Manuel A. Coimbra; Artur M. S. Silva; Susana M. Cardoso. Application of Hydroxytyrosol in the Functional Foods Field: From Ingredient to Dietary Supplements. Antioxidants 2020, 9, 1246 .
AMA StyleAndreia F. R. Silva, Daniela Resende, Mariana Monteiro, Manuel A. Coimbra, Artur M. S. Silva, Susana M. Cardoso. Application of Hydroxytyrosol in the Functional Foods Field: From Ingredient to Dietary Supplements. Antioxidants. 2020; 9 (12):1246.
Chicago/Turabian StyleAndreia F. R. Silva; Daniela Resende; Mariana Monteiro; Manuel A. Coimbra; Artur M. S. Silva; Susana M. Cardoso. 2020. "Application of Hydroxytyrosol in the Functional Foods Field: From Ingredient to Dietary Supplements." Antioxidants 9, no. 12: 1246.
The search for new natural antioxidants is a growing area of research due to the broad spectrum of their biological properties, which are associated with the prevention of chronic diseases that originate in oxidative stress
Susana Cardoso; Alessia Fazio. The Antioxidant Capacities of Natural Products 2019. Molecules 2020, 25, 5676 .
AMA StyleSusana Cardoso, Alessia Fazio. The Antioxidant Capacities of Natural Products 2019. Molecules. 2020; 25 (23):5676.
Chicago/Turabian StyleSusana Cardoso; Alessia Fazio. 2020. "The Antioxidant Capacities of Natural Products 2019." Molecules 25, no. 23: 5676.
Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from Fucus vesiculosus using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (v/v), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DWextract), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS•+ (IC50 of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC50 of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.
Sónia J. Amarante; Marcelo D. Catarino; Catarina Marçal; Artur M. S. Silva; Rita Ferreira; Susana M. Cardoso. Microwave-Assisted Extraction of Phlorotannins from Fucus vesiculosus. Marine Drugs 2020, 18, 559 .
AMA StyleSónia J. Amarante, Marcelo D. Catarino, Catarina Marçal, Artur M. S. Silva, Rita Ferreira, Susana M. Cardoso. Microwave-Assisted Extraction of Phlorotannins from Fucus vesiculosus. Marine Drugs. 2020; 18 (11):559.
Chicago/Turabian StyleSónia J. Amarante; Marcelo D. Catarino; Catarina Marçal; Artur M. S. Silva; Rita Ferreira; Susana M. Cardoso. 2020. "Microwave-Assisted Extraction of Phlorotannins from Fucus vesiculosus." Marine Drugs 18, no. 11: 559.
Due to their large spectrum of bioactive properties, much attention has recently been drawn to phlorotannins—i.e., phenolic compounds characteristic from brown macroalgae. This study aimed to evaluate the antioxidant and anti-inflammatory properties of F. vesiculosus phlorotannin extracts and purified fractions. Overall, the crude extract and its ethyl acetate fraction (EtOAc) showed good radical scavenging activity, particularly towards nitric oxide (NO•). Subsequent subfractions of EtOAc (F1 to F9) with different molecular weights were then shown to inhibit lipopolysaccharide-induced NO• production in macrophages, with stronger effects being observed for fractions of lower MWs. Of the three intracellular markers analyzed, inducible NO• synthase showed the highest sensitivity to almost all the phlorotannin-rich samples, followed by interleukin 1β and cyclooxygenase 2, which was only inhibited by F2. Furthermore, this subfraction inhibited the phosphorylation and degradation of inhibitory protein κBα, thus preventing the activation of NF-κB and blocking the inflammatory cascade at the transcriptional level. This sample was characterized by the presence of a major compound with a deprotonated molecular ion at m/z 507 with a fragmentation pattern coherent with that of a phlorotannin derivative. Overall, this work unveiled some of the mechanistic aspects behind the anti-inflammatory capacity of phlorotannins from F. vesiculosus, endorsing its use as a possible natural source of anti-inflammatory compounds.
Marcelo D. Catarino; Ana Silva; Maria T. Cruz; Nuno Mateus; Artur M. S. Silva; Susana M. Cardoso. Phlorotannins from Fucus vesiculosus: Modulation of Inflammatory Response by Blocking NF-κB Signaling Pathway. International Journal of Molecular Sciences 2020, 21, 6897 .
AMA StyleMarcelo D. Catarino, Ana Silva, Maria T. Cruz, Nuno Mateus, Artur M. S. Silva, Susana M. Cardoso. Phlorotannins from Fucus vesiculosus: Modulation of Inflammatory Response by Blocking NF-κB Signaling Pathway. International Journal of Molecular Sciences. 2020; 21 (18):6897.
Chicago/Turabian StyleMarcelo D. Catarino; Ana Silva; Maria T. Cruz; Nuno Mateus; Artur M. S. Silva; Susana M. Cardoso. 2020. "Phlorotannins from Fucus vesiculosus: Modulation of Inflammatory Response by Blocking NF-κB Signaling Pathway." International Journal of Molecular Sciences 21, no. 18: 6897.
Thymus genus comprises numerous species that are particularly abundant in the West Mediterranean region. A growing body of evidence suggests that many of these species are a rich source of bioactive compounds, including phenolic compounds such as rosmarinic acid, salvianolic acids and luteolin glycosides, able to render them potential applications in a range of industrial fields. This review collects the most relevant studies focused on the antioxidant, anti-inflammatory and anti-cancer of phenolic-rich extracts from Thymus plants, highlighting correlations made by the authors with respect to the main phenolic players in such activities.
Andrea F. Afonso; Olívia R. Pereira; Susana M. Cardoso. Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-Inflammatory and Antitumoral Properties. Antioxidants 2020, 9, 814 .
AMA StyleAndrea F. Afonso, Olívia R. Pereira, Susana M. Cardoso. Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-Inflammatory and Antitumoral Properties. Antioxidants. 2020; 9 (9):814.
Chicago/Turabian StyleAndrea F. Afonso; Olívia R. Pereira; Susana M. Cardoso. 2020. "Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-Inflammatory and Antitumoral Properties." Antioxidants 9, no. 9: 814.
Thymus algeriensis Bioss & Reut is an Algerian native plant traditionally used for culinary and medicinal purposes. To evaluate the in vivo antioxidant and antimicrobial properties of T. algeriensis, phenolic compounds were extracted using hydromethanolic solutions and administrated to Swiss albinos mice. It was observed that the plasma antioxidant capacity increased, as well as catalase and glutathione levels, whereas malondialdehyde decreased. In vitro assays confirmed that the extract scavenged 2,2-diphenyl-1-picrylhydrazyl, DPPH• (7 µg/mL), chelated (EC50: 512 µg/mL) and reduced iron ions (5.3 mM FeSO4/g), and inhibited β-carotene bleaching (90% at 2 mg/mL). Antibacterial effects were also observed towards Salmonella typhimurium and Proteus mirabilis. However, the methanolic fraction obtained by reversed solid phase cartridge, showed antibacterial activity against Escherichia coli (MIC = 9.37 mg/mL), Proteus mirabilis (MIC = 4.68 mg/mL), Salmonella typhimurium (MIC = 7.06 mg/mL), Micrococcus luteus (MIC = 7.03 mg/mL), and Bacillus cereus (MIC = 2.34 mg/mL). UHPLC-DAD-ESI-MSn analysis showed that these properties could result from rosmarinic acid, caffeoyl rosmaniric acid, and kaempferol and eriodictyol glycoside derivatives. These results pave the way for the understanding of T. algeriensis traditional applications and its use for the development of novel food applications.
Nadjat Righi; Sabah Boumerfeg; Pedro A.R. Fernandes; Amirouche Deghima; Faiza Baali; Elisabete Coelho; Susana M. Cardoso; Manuel A. Coimbra; Abderrahmane Baghiani. Thymus algeriensis Bioss & Reut: Relationship of phenolic compounds composition with in vitro/in vivo antioxidant and antibacterial activity. Food Research International 2020, 136, 109500 .
AMA StyleNadjat Righi, Sabah Boumerfeg, Pedro A.R. Fernandes, Amirouche Deghima, Faiza Baali, Elisabete Coelho, Susana M. Cardoso, Manuel A. Coimbra, Abderrahmane Baghiani. Thymus algeriensis Bioss & Reut: Relationship of phenolic compounds composition with in vitro/in vivo antioxidant and antibacterial activity. Food Research International. 2020; 136 ():109500.
Chicago/Turabian StyleNadjat Righi; Sabah Boumerfeg; Pedro A.R. Fernandes; Amirouche Deghima; Faiza Baali; Elisabete Coelho; Susana M. Cardoso; Manuel A. Coimbra; Abderrahmane Baghiani. 2020. "Thymus algeriensis Bioss & Reut: Relationship of phenolic compounds composition with in vitro/in vivo antioxidant and antibacterial activity." Food Research International 136, no. : 109500.
The recently described species Myxacorys almedinensis and two other cyanobacteria were isolated from the limestone walls of the Old Cathedral of Coimbra, Portugal (UNESCO World Heritage Site). The high-quality genome sequences presented here will be essential for characterization purposes and description of the novel taxa.
Fabiana Soares; João Trovão; Catarina Coelho; Inês Costa; Nuno Mesquita; Francisco Gil; Lídia Catarino; Susana M. Cardoso; António Portugal; Igor Tiago. High-Quality Draft Genome Sequences of Three Cyanobacteria Isolated from the Limestone Walls of the Old Cathedral of Coimbra, Portugal. Microbiology Resource Announcements 2020, 9, 1 .
AMA StyleFabiana Soares, João Trovão, Catarina Coelho, Inês Costa, Nuno Mesquita, Francisco Gil, Lídia Catarino, Susana M. Cardoso, António Portugal, Igor Tiago. High-Quality Draft Genome Sequences of Three Cyanobacteria Isolated from the Limestone Walls of the Old Cathedral of Coimbra, Portugal. Microbiology Resource Announcements. 2020; 9 (26):1.
Chicago/Turabian StyleFabiana Soares; João Trovão; Catarina Coelho; Inês Costa; Nuno Mesquita; Francisco Gil; Lídia Catarino; Susana M. Cardoso; António Portugal; Igor Tiago. 2020. "High-Quality Draft Genome Sequences of Three Cyanobacteria Isolated from the Limestone Walls of the Old Cathedral of Coimbra, Portugal." Microbiology Resource Announcements 9, no. 26: 1.
Green macroalgae of the genus Ulva, commonly known as sea lettuces, are currently traded worldwide for multiple applications, including human consumption. In this work, we evaluated the seasonal variation of the total content of lipids, esterified fatty acids (FAs) and polar lipids (the major carriers of FAs) at the level of the classes and molecular species of Ulva rigida cultivated in Portugal in a land-based integrated multitrophic aquaculture (IMTA) system. Comparing winter, spring, summer and autumn samples, the lipid content and abundance of unsaturated FAs, namely n-3 polyunsaturated FAs, were highest in winter and lowest in summer. However, regardless of seasonal variations, U. rigida had a low n-6/n-3 ratio in all seasons, which is associated with health benefits. Among the polar lipids, those that varied the most were betaines, glycolipids and phospholipids, including a high number of lysolipid species that increased during autumn and spring. Multivariate analysis using principal component analysis (PCA) showed better discrimination of the four seasons when the dataset on molecular lipid species was used. Knowledge of the seasonal variability of lipid composition in biomass of Ulva rigida can be used to better explore these green macroalgae as a source of bioactive polar lipids with high market value.
Ana S.P. Moreira; Elisabete da Costa; Tânia Melo; Ronan Sulpice; Susana M. Cardoso; Bárbara Pitarma; Rui Pereira; Maria H. Abreu; Pedro Domingues; Ricardo Calado; M. Rosário Domingues. Seasonal plasticity of the polar lipidome of Ulva rigida cultivated in a sustainable integrated multi-trophic aquaculture. Algal Research 2020, 49, 101958 .
AMA StyleAna S.P. Moreira, Elisabete da Costa, Tânia Melo, Ronan Sulpice, Susana M. Cardoso, Bárbara Pitarma, Rui Pereira, Maria H. Abreu, Pedro Domingues, Ricardo Calado, M. Rosário Domingues. Seasonal plasticity of the polar lipidome of Ulva rigida cultivated in a sustainable integrated multi-trophic aquaculture. Algal Research. 2020; 49 ():101958.
Chicago/Turabian StyleAna S.P. Moreira; Elisabete da Costa; Tânia Melo; Ronan Sulpice; Susana M. Cardoso; Bárbara Pitarma; Rui Pereira; Maria H. Abreu; Pedro Domingues; Ricardo Calado; M. Rosário Domingues. 2020. "Seasonal plasticity of the polar lipidome of Ulva rigida cultivated in a sustainable integrated multi-trophic aquaculture." Algal Research 49, no. : 101958.
Scrophularia tenuipes is an Algerian-Tunisian endemic species, which has not been studied yet. Ethyl acetate (EA) and n-butanol (Bu) fractions obtained from Scrophularia tenuipes were investigated for their health benefit properties, in particular with respect to in vivo/in vitro anti-inflammatory and antioxidant activities, as well as their potential to inhibit key enzymes with impact in diabetes (α-glucosidase and α-amylase). The fractions had a distinct phytochemical composition, of which EA was richer in total phenolic compounds (225 mg GAE/g) and mostly composed of the phenylethanoid acetyl martynoside. Compared to EA, Bu had higher amounts of total flavonoids, and according to the result obtained from UHPLC-DAD-ESI-MSn analysis, harpagoside (iridoid) was its major phytochemical. EA fraction was quite promising with regard to the in vivo (at 200 mg/kg, po) anti-inflammatory effect (62% and 52% for carrageenan-induced rat paw edema and xylene-induced ear edema tests, respectively), while Bu fraction exhibited a stronger antioxidant capacity in all tests (IC50 = 68 µg/mL, IC50 = 18 µg/mL, IC50 = 18 µg/mL and A0.50 = 43 µg/mL for DPPH●, ABTS•+, O2•− scavenging assays and cupric-reducing antioxidant capacity method, respectively). Both fractions also showed a strong effect against α-amylase enzyme (IC50 = 8 µg/mL and 10 µg/mL for EA and Bu fraction, respectively).
Zeyneb Chaibeddra; Salah Akkal; Houria Ouled-Haddar; Artur M. S. Silva; Ammar Zellagui; Mohamed Sebti; Susana M. Cardoso. Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities. Molecules 2020, 25, 1647 .
AMA StyleZeyneb Chaibeddra, Salah Akkal, Houria Ouled-Haddar, Artur M. S. Silva, Ammar Zellagui, Mohamed Sebti, Susana M. Cardoso. Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities. Molecules. 2020; 25 (7):1647.
Chicago/Turabian StyleZeyneb Chaibeddra; Salah Akkal; Houria Ouled-Haddar; Artur M. S. Silva; Ammar Zellagui; Mohamed Sebti; Susana M. Cardoso. 2020. "Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities." Molecules 25, no. 7: 1647.