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Dr. M Rosario Domingues
Mass Spectrometry Centre and Centre for Environmental and Marine Studies, CESAM, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal

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0 Glycomics
0 Lipidomics
0 Mass Spectrometry
0 Lipid oxidation
0 Marine lipidiomics

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Mass Spectrometry
Lipidomics
Lipid oxidation

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Journal article
Published: 22 August 2021 in Marine Drugs
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The present study aimed to contrast the fatty acid (FA) profile of ascidians (Ascidiacea) and seaweeds (sea lettuce, Ulva spp. and bladderwrack, Fucus sp.) occurring in a coastal lagoon with versus without the influence of organic-rich effluents from fish farming activities. Our results revealed that ascidians and seaweeds from these contrasting environments displayed significant differences in their FA profiles. The n-3/n-6 ratio of Ascidiacea was lower under the influence of fish farming conditions, likely a consequence of the growing level of terrestrial-based ingredients rich on n-6 FA used in the formulation of aquafeeds. Unsurprisingly, these specimens also displayed significantly higher levels of 18:1(n-7+n-9) and 18:2n-6, as these combined accounted for more than 50% of the total pool of FAs present in formulated aquafeeds. The dissimilarities recorded in the FAs of seaweeds from these different environments were less marked (≈5%), with these being more pronounced in the FA classes of the brown seaweed Fucus sp. (namely PUFA). Overall, even under the influence of organic-rich effluents from fish farming activities, ascidians and seaweeds are a valuable source of health-promoting FAs, which confirms their potential for sustainable farming practices, such as integrated multi-trophic aquaculture.

ACS Style

Luísa Marques; Maria Rosário Domingues; Elisabete da Costa; Maria Helena Abreu; Ana Isabel Lillebø; Ricardo Calado. Screening for Health-Promoting Fatty Acids in Ascidians and Seaweeds Grown under the Influence of Fish Farming Activities. Marine Drugs 2021, 19, 469 .

AMA Style

Luísa Marques, Maria Rosário Domingues, Elisabete da Costa, Maria Helena Abreu, Ana Isabel Lillebø, Ricardo Calado. Screening for Health-Promoting Fatty Acids in Ascidians and Seaweeds Grown under the Influence of Fish Farming Activities. Marine Drugs. 2021; 19 (8):469.

Chicago/Turabian Style

Luísa Marques; Maria Rosário Domingues; Elisabete da Costa; Maria Helena Abreu; Ana Isabel Lillebø; Ricardo Calado. 2021. "Screening for Health-Promoting Fatty Acids in Ascidians and Seaweeds Grown under the Influence of Fish Farming Activities." Marine Drugs 19, no. 8: 469.

Journal article
Published: 13 August 2021 in International Journal of Molecular Sciences
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Chronic UV radiation causes oxidative stress and inflammation of skin and blood cells. Therefore, in this study, we assessed the effects of cannabidiol (CBD), a natural phytocannabinoid with antioxidant and anti-inflammatory properties, on the phospholipid (PL) and ceramide (CER) profiles in the plasma of nude rats irradiated with UVA/UVB and treated topically with CBD. The results obtained showed that UVA/UVB radiation increased the levels of phosphatidylcholines, lysophospholipids, and eicosanoids (PGE2, TxB2), while downregulation of sphingomyelins led to an increase in CER[NS] and CER[NDS]. Topical application of CBD to the skin of control rats significantly upregulated plasma ether-linked phosphatidylethanolamines (PEo) and ceramides. However, CBD administered to rats irradiated with UVA/UVB promoted further upregulation of CER and PEo and led to significant downregulation of lysophospholipids. This was accompanied by the anti-inflammatory effect of CBD, manifested by a reduction in the levels of proinflammatory PGE2 and TxB2 and a dramatic increase in the level of anti-inflammatory LPXA4. It can therefore be suggested that topical application of CBD to the skin of rats exposed to UVA/UVB radiation prevents changes in plasma phospholipid profile resulting in a reduction of inflammation by reducing the level of LPE and LPC species and increasing antioxidant capacity due to upregulation of PEo species.

ACS Style

Wojciech Łuczaj; Anna Jastrząb; Maria Do Rosário Domingues; Pedro Domingues; Elżbieta Skrzydlewska. Changes in Phospholipid/Ceramide Profiles and Eicosanoid Levels in the Plasma of Rats Irradiated with UV Rays and Treated Topically with Cannabidiol. International Journal of Molecular Sciences 2021, 22, 8700 .

AMA Style

Wojciech Łuczaj, Anna Jastrząb, Maria Do Rosário Domingues, Pedro Domingues, Elżbieta Skrzydlewska. Changes in Phospholipid/Ceramide Profiles and Eicosanoid Levels in the Plasma of Rats Irradiated with UV Rays and Treated Topically with Cannabidiol. International Journal of Molecular Sciences. 2021; 22 (16):8700.

Chicago/Turabian Style

Wojciech Łuczaj; Anna Jastrząb; Maria Do Rosário Domingues; Pedro Domingues; Elżbieta Skrzydlewska. 2021. "Changes in Phospholipid/Ceramide Profiles and Eicosanoid Levels in the Plasma of Rats Irradiated with UV Rays and Treated Topically with Cannabidiol." International Journal of Molecular Sciences 22, no. 16: 8700.

Journal article
Published: 26 July 2021 in Marine Drugs
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Grateloupia turuturu Yamada, 1941, is a red seaweed widely used for food in Japan and Korea which was recorded on the Atlantic Coast of Europe about twenty years ago. This seaweed presents eicosapentaenoic acid (EPA) and other polyunsaturated fatty acids (PUFAs) in its lipid fraction, a feature that sparked the interest on its potential applications. In seaweeds, PUFAs are mostly esterified to polar lipids, emerging as healthy phytochemicals. However, to date, these biomolecules are still unknown for G. turuturu. The present work aimed to identify the polar lipid profile of G. turuturu, using modern lipidomics approaches based on high performance liquid chromatography coupled to high resolution mass spectrometry (LC–MS) and gas chromatography coupled to mass spectrometry (GC–MS). The health benefits of polar lipids were identified by health lipid indices and the assessment of antioxidant and anti-inflammatory activities. The polar lipids profile identified from G. turuturu included 205 lipid species distributed over glycolipids, phospholipids, betaine lipids and phosphosphingolipids, which featured a high number of lipid species with EPA and PUFAs. The nutritional value of G. turuturu has been shown by its protein content, fatty acyl composition and health lipid indices, thus confirming G. turuturu as an alternative source of protein and lipids. Some of the lipid species assigned were associated to biological activity, as polar lipid extracts showed antioxidant activity evidenced by free radical scavenging potential for the 2,2′-azino-bis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS+) radical (IC50 ca. 130.4 μg mL−1) and for the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (IC25 ca. 129.1 μg mL−1) and anti-inflammatory activity by inhibition of the COX-2 enzyme (IC50 ca. 33 µg mL−1). Both antioxidant and anti-inflammatory activities were detected using a low concentration of extracts. This integrative approach contributes to increase the knowledge of G. turuturu as a species capable of providing nutrients and bioactive molecules with potential applications in the nutraceutical, pharmaceutical and cosmeceutical industries.

ACS Style

Elisabete da Costa; Tânia Melo; Mariana Reis; Pedro Domingues; Ricardo Calado; Maria Abreu; Maria Domingues. Polar Lipids Composition, Antioxidant and Anti-Inflammatory Activities of the Atlantic Red Seaweed Grateloupia turuturu. Marine Drugs 2021, 19, 414 .

AMA Style

Elisabete da Costa, Tânia Melo, Mariana Reis, Pedro Domingues, Ricardo Calado, Maria Abreu, Maria Domingues. Polar Lipids Composition, Antioxidant and Anti-Inflammatory Activities of the Atlantic Red Seaweed Grateloupia turuturu. Marine Drugs. 2021; 19 (8):414.

Chicago/Turabian Style

Elisabete da Costa; Tânia Melo; Mariana Reis; Pedro Domingues; Ricardo Calado; Maria Abreu; Maria Domingues. 2021. "Polar Lipids Composition, Antioxidant and Anti-Inflammatory Activities of the Atlantic Red Seaweed Grateloupia turuturu." Marine Drugs 19, no. 8: 414.

Journal article
Published: 24 July 2021 in Biomedicines
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Alzheimer’s disease (AD) is characterized by the accumulation of extracellular plaques composed by amyloid-β (Aβ) and intracellular neurofibrillary tangles of hyperphosphorylated tau. AD-related neurodegenerative mechanisms involve early changes of mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) and impairment of cellular events modulated by these subcellular domains. In this study, we characterized the structural and functional alterations at MAM, mitochondria, and ER/microsomes in a mouse neuroblastoma cell line (N2A) overexpressing the human amyloid precursor protein (APP) with the familial Swedish mutation (APPswe). Proteins levels were determined by Western blot, ER-mitochondria contacts were quantified by transmission electron microscopy, and Ca2+ homeostasis and mitochondria function were analyzed using fluorescent probes and Seahorse assays. In this in vitro AD model, we found APP accumulated in MAM and mitochondria, and altered levels of proteins implicated in ER-mitochondria tethering, Ca2+ signaling, mitochondrial dynamics, biogenesis and protein import, as well as in the stress response. Moreover, we observed a decreased number of close ER-mitochondria contacts, activation of the ER unfolded protein response, reduced Ca2+ transfer from ER to mitochondria, and impaired mitochondrial function. Together, these results demonstrate that several subcellular alterations occur in AD-like neuronal cells, which supports that the defective ER-mitochondria crosstalk is an important player in AD physiopathology.

ACS Style

Tânia Fernandes; Rosa Resende; Diana Silva; Ana Marques; Armanda Santos; Sandra Cardoso; M. Domingues; Paula Moreira; Cláudia Pereira. Structural and Functional Alterations in Mitochondria-Associated Membranes (MAMs) and in Mitochondria Activate Stress Response Mechanisms in an In Vitro Model of Alzheimer’s Disease. Biomedicines 2021, 9, 881 .

AMA Style

Tânia Fernandes, Rosa Resende, Diana Silva, Ana Marques, Armanda Santos, Sandra Cardoso, M. Domingues, Paula Moreira, Cláudia Pereira. Structural and Functional Alterations in Mitochondria-Associated Membranes (MAMs) and in Mitochondria Activate Stress Response Mechanisms in an In Vitro Model of Alzheimer’s Disease. Biomedicines. 2021; 9 (8):881.

Chicago/Turabian Style

Tânia Fernandes; Rosa Resende; Diana Silva; Ana Marques; Armanda Santos; Sandra Cardoso; M. Domingues; Paula Moreira; Cláudia Pereira. 2021. "Structural and Functional Alterations in Mitochondria-Associated Membranes (MAMs) and in Mitochondria Activate Stress Response Mechanisms in an In Vitro Model of Alzheimer’s Disease." Biomedicines 9, no. 8: 881.

Journal article
Published: 17 July 2021 in Redox Biology
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Hydrogen peroxide (H2O2) is widely used in clinical practice due to its antiseptic properties and its ability to heal wounds. However, due to its involvement in the formation of ROS, H2O2 causes several side effects, including disorders of the metabolism of skin cells and the development of chronic inflammation mediated by oxidative stress. Therefore, this study evaluated the effects of cannabidiol (CBD), a phytocannabinoid known for its antioxidant and anti-inflammatory properties, on the proteome of keratinocyte membranes exposed to H2O2. Overall, the hydrogen peroxide caused the levels of several proteins to increase, while the treatment with CBD prevented these changes. Analysis of the protein-protein interaction network showed that the significant changes mainly involved proteins with important roles in the proteasomal activity, protein folding processes (regulatory subunit of the proteasome 26S 6A, beta proteasome subunit type 1, chaperonin 60 kDa), protein biosynthesis (40S ribosomal proteins S16, S2 and ubiquitin-S27a), regulation of the redox balance (carbonyl reductase [NADPH] 1 and NAD(P)H [quinone] 1 dehydrogenase) and cell survival (14-3-3 theta protein). Additionally, CBD reduced the total amount of MDA, 4-HNE and 4-ONE-protein adducts. Therefore, we conclude that CBD partially prevents the changes induced by hydrogen peroxide by reducing oxidative stress and maintaining proteostasis networks. Moreover, our results indicate that combination therapy with CBD may bring a promising approach in the clinical use of hydrogen peroxide by preventing its pro-oxidative and pro-inflammatory effect through potential participation of CBD in membrane mediated molecular signaling.

ACS Style

Sinemyiz Atalay; Agnieszka Gęgotek; Pedro Domingues; Elżbieta Skrzydlewska. Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network. Redox Biology 2021, 46, 102074 .

AMA Style

Sinemyiz Atalay, Agnieszka Gęgotek, Pedro Domingues, Elżbieta Skrzydlewska. Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network. Redox Biology. 2021; 46 ():102074.

Chicago/Turabian Style

Sinemyiz Atalay; Agnieszka Gęgotek; Pedro Domingues; Elżbieta Skrzydlewska. 2021. "Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network." Redox Biology 46, no. : 102074.

Journal article
Published: 24 June 2021 in Current Nutrition & Food Science
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Background: Some plant seeds and berries have gained increased recognition due to their wide variety of bioactive compounds. Many of these foods are rich in lipids with high nutritional value, of which n-3 and n-6 essential fatty acids stand out. However, knowledge of these foodstuffs’ chemical composition and biological activity and their value as a source of healthy lipids is far from being fully explored. Objective and Methods: The benefits of the intake of hemp seeds, flaxseed and açaí berries, considered functional foods, have been compiled in this review. Likewise, their general chemical composition will be described to evaluate their relevance in a healthy diet, highlighting their lipid components and nutritional lipid indices. Results and Conclusion: The fibres and essential fatty acids of hemp seeds and flaxseed and the antioxidant properties of açaí berries provide them protective roles against several chronic non-communicable diseases and represent significant beneficial effects that add value to these healthy natural products. More research is needed to deepen the knowledge on their lipids’ molecular composition and bioactivity.

ACS Style

Pedro Magalhães; Rosário M. Domingues; Eliana Alves. Hemp seeds, flaxseed and açaí berries: Health benefits and nutritional importance with emphasis on the lipid content. Current Nutrition & Food Science 2021, 17, 1 -1.

AMA Style

Pedro Magalhães, Rosário M. Domingues, Eliana Alves. Hemp seeds, flaxseed and açaí berries: Health benefits and nutritional importance with emphasis on the lipid content. Current Nutrition & Food Science. 2021; 17 ():1-1.

Chicago/Turabian Style

Pedro Magalhães; Rosário M. Domingues; Eliana Alves. 2021. "Hemp seeds, flaxseed and açaí berries: Health benefits and nutritional importance with emphasis on the lipid content." Current Nutrition & Food Science 17, no. : 1-1.

Journal article
Published: 23 June 2021 in Marine Drugs
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The demand for sustainable and environmentally friendly food sources and food ingredients is increasing, and microalgae are promoted as a sustainable source of essential and bioactive lipids, with high levels of omega-3 fatty acids (ω-3 FA), comparable to those of fish. However, most FA screening studies on algae are scattered or use different methodologies, preventing a true comparison of its content between microalgae. In this work, we used gas-chromatography mass-spectrometry (GC-MS) to characterize the FA profile of seven different commercial microalgae with biotechnological applications (Chlorella vulgaris, Chlorococcum amblystomatis, Scenedesmus obliquus, Tetraselmis chui, Phaeodactylum tricornutum, Spirulina sp., and Nannochloropsis oceanica). Screening for antioxidant activity was also performed to understand the relationship between FA profile and bioactivity. Microalgae exhibited specific FA profiles with a different composition, namely in the ω-3 FA profile, but with species of the same phylum showing similar tendencies. The different lipid extracts showed similar antioxidant activities, but with a low activity of the extracts of Nannochloropsis oceanica. Overall, this study provides a direct comparison of FA profiles between microalgae species, supporting the role of these species as alternative, sustainable, and healthy sources of essential lipids.

ACS Style

Tiago Conde; Bruna Neves; Daniela Couto; Tânia Melo; Bruno Neves; Margarida Costa; Joana Silva; Pedro Domingues; M. Domingues. Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity. Marine Drugs 2021, 19, 357 .

AMA Style

Tiago Conde, Bruna Neves, Daniela Couto, Tânia Melo, Bruno Neves, Margarida Costa, Joana Silva, Pedro Domingues, M. Domingues. Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity. Marine Drugs. 2021; 19 (7):357.

Chicago/Turabian Style

Tiago Conde; Bruna Neves; Daniela Couto; Tânia Melo; Bruno Neves; Margarida Costa; Joana Silva; Pedro Domingues; M. Domingues. 2021. "Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity." Marine Drugs 19, no. 7: 357.

Short communication
Published: 10 June 2021 in Biochemistry and Biophysics Reports
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Acute myocardial infarction (AMI) is the leading cause of death, morbidity, and health costs worldwide. In AMI, a sudden blockage of blood flow causes myocardial ischemia and cell death. Reperfusion after ischemia has paradoxical effects and may exacerbate the myocardial injury, a process known as ischemic reperfusion injury. In this work we evaluated the lipidome of isolated rat hearts, maintained in controlled perfusion (CT), undergoing global ischemia (ISC) or ischemia followed by reperfusion (IR). 153 polar lipid levels were significantly different between conditions. 48 features had q < 0.001 and included 8 phosphatidylcholines and 4 lysophospholipids, which were lower in ISC compared to CT, and even lower in the IR group, suggesting that IR induces more profound changes than ISC. We observed that the levels of 16 alkyl acyl phospholipids were significantly altered during ISC and IR. Overall, these data indicate that myocardial lipid remodelling and possibly damage occurs to a greater extent during reperfusion. The adaptation of cardiac lipidome during ISC and IR described is consistent with the presence of oxidative damage and may reflect the impact of AMI on the lipidome at the cellular level and provide new insights into the role of lipids in the pathophysiology of acute myocardial ischemia/reperfusion injury.

ACS Style

Javier-Fernando Montero-Bullon; Susana S. Aveiro; Tânia Melo; Tânia Martins-Marques; Diana Lopes; Bruna Neves; Henrique Girão; M. Rosário M Domingues; Pedro Domingues. Cardiac phospholipidome is altered during ischemia and reperfusion in an ex vivo rat model. Biochemistry and Biophysics Reports 2021, 27, 101037 .

AMA Style

Javier-Fernando Montero-Bullon, Susana S. Aveiro, Tânia Melo, Tânia Martins-Marques, Diana Lopes, Bruna Neves, Henrique Girão, M. Rosário M Domingues, Pedro Domingues. Cardiac phospholipidome is altered during ischemia and reperfusion in an ex vivo rat model. Biochemistry and Biophysics Reports. 2021; 27 ():101037.

Chicago/Turabian Style

Javier-Fernando Montero-Bullon; Susana S. Aveiro; Tânia Melo; Tânia Martins-Marques; Diana Lopes; Bruna Neves; Henrique Girão; M. Rosário M Domingues; Pedro Domingues. 2021. "Cardiac phospholipidome is altered during ischemia and reperfusion in an ex vivo rat model." Biochemistry and Biophysics Reports 27, no. : 101037.

Preprint content
Published: 26 May 2021
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Male and female offspring of obese mothers are known to differ significantly in their metabolic adaptation and later development of complications. We investigated the sex-dependent responses in obese offspring of mice with maternal obesity, focusing on changes in liver glucose and lipid metabolism. Maternal obesity prior to and during gestation led to hepatic insulin resistance and inflammation in male offspring, while female offspring were protected. These sex differences were explained by more efficient transcriptional and posttranscriptional reprogramming of metabolic pathways to prevent the damaging effects of maternal obesity in females compared to males. These differences were sustained later in life, resulting in a better metabolic balance in female offspring. In conclusion, sex and maternal obesity drive transcriptional and posttranscriptional regulation of major metabolic processes in offspring liver differently, explaining the sexual dimorphism in obesity-associated metabolic risk.

ACS Style

Christina Savva; Luisa A. Helguero; Marcela González-Granillo; Tânia Melo; Daniela Couto; Bo Angelin; Maria Rosário Domingues; Xidan Li; Claudia Kutter; Marion Korach-André. Molecular programming in utero modulates hepatic lipid metabolism and adult metabolic risk in obese mother offspring in a sex-specific manner. 2021, 1 .

AMA Style

Christina Savva, Luisa A. Helguero, Marcela González-Granillo, Tânia Melo, Daniela Couto, Bo Angelin, Maria Rosário Domingues, Xidan Li, Claudia Kutter, Marion Korach-André. Molecular programming in utero modulates hepatic lipid metabolism and adult metabolic risk in obese mother offspring in a sex-specific manner. . 2021; ():1.

Chicago/Turabian Style

Christina Savva; Luisa A. Helguero; Marcela González-Granillo; Tânia Melo; Daniela Couto; Bo Angelin; Maria Rosário Domingues; Xidan Li; Claudia Kutter; Marion Korach-André. 2021. "Molecular programming in utero modulates hepatic lipid metabolism and adult metabolic risk in obese mother offspring in a sex-specific manner." , no. : 1.

Journal article
Published: 21 April 2021 in Foods
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Macroalgae of the genus Ulva have long been used as human food. Local environmental conditions, among other factors, can have an impact on their nutrient and phytochemical composition, as well as on the value of the seaweed for food and non-food applications. This study is the first to initiate a comparison between commercial Ulva spp. from different European origins, France (FR, wild-harvested Ulva spp.), and Portugal (PT, farm-raised Ulva rigida), in terms of proximate composition, esterified fatty acids (FA), and polar lipids. The ash content was higher in PT samples, while FR samples had higher levels of proteins, lipids, and carbohydrates and other compounds. The profile of esterified FA, as well as FA-containing polar lipids at the class and species levels were also significantly different. The FR samples showed about three-fold higher amount of n-3 polyunsaturated FA, while PT samples showed two-fold higher content of monounsaturated FA. Quantification of glycolipids and phospholipids revealed, respectively, two-fold and three-fold higher levels in PT samples. Despite the differences found, the polar lipids identified in both batches included some lipid species with recognized bioactivity, valuing Ulva biomass with functional properties, increasing their added value, and promoting new applications, namely in nutraceutical and food markets.

ACS Style

Ana Moreira; Elisabete da Costa; Tânia Melo; Diana Lopes; Adriana Pais; Sónia Santos; Bárbara Pitarma; Madalena Mendes; Maria Abreu; Pi Collén; Pedro Domingues; M. Domingues. Polar Lipids of Commercial Ulva spp. of Different Origins: Profiling and Relevance for Seaweed Valorization. Foods 2021, 10, 914 .

AMA Style

Ana Moreira, Elisabete da Costa, Tânia Melo, Diana Lopes, Adriana Pais, Sónia Santos, Bárbara Pitarma, Madalena Mendes, Maria Abreu, Pi Collén, Pedro Domingues, M. Domingues. Polar Lipids of Commercial Ulva spp. of Different Origins: Profiling and Relevance for Seaweed Valorization. Foods. 2021; 10 (5):914.

Chicago/Turabian Style

Ana Moreira; Elisabete da Costa; Tânia Melo; Diana Lopes; Adriana Pais; Sónia Santos; Bárbara Pitarma; Madalena Mendes; Maria Abreu; Pi Collén; Pedro Domingues; M. Domingues. 2021. "Polar Lipids of Commercial Ulva spp. of Different Origins: Profiling and Relevance for Seaweed Valorization." Foods 10, no. 5: 914.

Research article
Published: 05 April 2021 in Journal of Proteome Research
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Phenylketonuria (PKU) is a disease of the catabolism of phenylalanine (Phe), caused by an impaired function of the enzyme phenylalanine hydroxylase. Therapeutics is based on the restriction of Phe intake, which mostly requires a modification of the diet. Dietary restrictions can lead to imbalances in specific nutrients, including lipids. In the present study, the plasma phospholipidome of PKU and healthy children (CT) was analyzed by hydrophilic interaction liquid chromatography–tandem mass spectrometry and gas chromatography–mass spectrometry. Using this approach, 187 lipid species belonging to nine different phospholipid classes and three ceramides were identified. Principal component analysis of the lipid species data set showed a distinction between PKU and CT groups. Univariate analysis revealed that 146 species of phospholipids were significantly different between both groups. Lipid species showing significant variation included phosphatidylcholines, containing polyunsaturated fatty acids (PUFA), which were more abundant in PKU. The high level of PUFA-containing lipid species in children with PKU may be related to a diet supplemented with PUFA. This study was the first report comparing the plasma polar lipidome of PKU and healthy children, highlighting that the phospholipidome of PKU children is significantly altered compared to CT. However, further studies with larger cohorts are needed to clarify whether these changes are specific to phenylketonuric children.

ACS Style

Inês M. S. Guerra; Luísa Diogo; Marisa Pinho; Tânia Melo; Pedro Domingues; M. Rosário Domingues; Ana S. P. Moreira. Plasma Phospholipidomic Profile Differs between Children with Phenylketonuria and Healthy Children. Journal of Proteome Research 2021, 20, 2651 -2661.

AMA Style

Inês M. S. Guerra, Luísa Diogo, Marisa Pinho, Tânia Melo, Pedro Domingues, M. Rosário Domingues, Ana S. P. Moreira. Plasma Phospholipidomic Profile Differs between Children with Phenylketonuria and Healthy Children. Journal of Proteome Research. 2021; 20 (5):2651-2661.

Chicago/Turabian Style

Inês M. S. Guerra; Luísa Diogo; Marisa Pinho; Tânia Melo; Pedro Domingues; M. Rosário Domingues; Ana S. P. Moreira. 2021. "Plasma Phospholipidomic Profile Differs between Children with Phenylketonuria and Healthy Children." Journal of Proteome Research 20, no. 5: 2651-2661.

Research article
Published: 15 March 2021 in Journal of Agricultural and Food Chemistry
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Frying allows cooking food while promoting their organoleptic properties, imparting crunchiness and flavor. The drawback is the oxidation of triacylglycerides (TAGs), leading to the formation of primary oxidized TAGs. Although they have been associated with chronic and degenerative diseases, they are precursors of pleasant flavors in fried foods. Nevertheless, there is a lack of knowledge about the oxidation species present in foods and their involvement in positive/negative health effects. In this work, high-resolution (HR) C30 reversed-phase (RP)–liquid chromatography (LC)–tandem HR mass spectrometry (MS/MS) was used to identify primary oxidation TAGs resulting from heating triolein (160 °C, 5 min). This allows simulating the initial heating process of frying oils usually used to prepare fried foods, such as chips, crisps, and snacks. Beyond hydroxy, dihydroxy, hydroperoxy, and hydroxy–hydroperoxy derivatives, already reported in phospholipids oxidized by Fenton reaction, new compounds were identified, such as dihydroxy–hydroperoxy–triolein derivatives and positional isomers (9/10- and 9/12-dihydroxy–triolein derivatives). These compounds should be considered when proposing flavor formation pathways and/or mitigating lipid-derived reactive oxygen species occurring during food frying.

ACS Style

Sílvia Petronilho; Bruna Neves; Tânia Melo; Sara Oliveira; Eliana Alves; Cristina Barros; Fernando M. Nunes; Manuel A. Coimbra; M. Rosário Domingues. Characterization of Non-volatile Oxidation Products Formed from Triolein in a Model Study at Frying Temperature. Journal of Agricultural and Food Chemistry 2021, 69, 3466 -3478.

AMA Style

Sílvia Petronilho, Bruna Neves, Tânia Melo, Sara Oliveira, Eliana Alves, Cristina Barros, Fernando M. Nunes, Manuel A. Coimbra, M. Rosário Domingues. Characterization of Non-volatile Oxidation Products Formed from Triolein in a Model Study at Frying Temperature. Journal of Agricultural and Food Chemistry. 2021; 69 (11):3466-3478.

Chicago/Turabian Style

Sílvia Petronilho; Bruna Neves; Tânia Melo; Sara Oliveira; Eliana Alves; Cristina Barros; Fernando M. Nunes; Manuel A. Coimbra; M. Rosário Domingues. 2021. "Characterization of Non-volatile Oxidation Products Formed from Triolein in a Model Study at Frying Temperature." Journal of Agricultural and Food Chemistry 69, no. 11: 3466-3478.

Journal article
Published: 06 March 2021 in Algal Research
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The valorization of seaweeds has generated unprecedented interest in recent years due to their unique chemical characteristics. The taxonomic differentiation between Chlorophyta, Rhodophyta, and Ochrophyta is often supported by their contrasting chemical characteristics. Polar lipids are the most abundant class of lipids in several seaweeds species often labelled as valuable phytochemicals. Nevertheless, little is still known about how the polar lipidome of seaweeds varies within and among different phyla. In this study, the polar lipidome of six seaweeds farmed under controlled conditions in a land-based integrated multitrophic aquaculture (IMTA), belonging to Chlorophyta (Ulva rigida and Codium tomentosum), Rhodophyta (Gracilaria gracilis, Palmaria palmata and Porphyra dioica), and Ochrophyta (Fucus vesiculosus) was characterized using state of the art high-resolution LC-MS analysis. The seaweeds were harvested during the same season and the same time window to control the biotic and abiotic shaping of their polar lipidome. In total, 477 different species of polar lipids (m/z values) were identified and distributed over the six seaweeds surveyed. Only a small group of 35 species of polar lipids were shared among all the seaweed species analyzed in this study. The distribution of the polar lipid species and lipid classes showed a trend towards grouping by phylum although seaweed specie specific polar lipid signatures can be assigned. Matching the data gathered in the present study with those previously acquired showed a species-specific clustering, which confirm the uniqueness of these natural biochemical fingerprints. Recognition of the specific lipidome is necessary because polar lipids are assigned with important bioactive properties. This knowledge will allow designing new strategies for chemical tuning during farming so that the yield of target compounds can be improved, and these valuable living resources can fully contribute to the sustainable growth of a blue bioeconomy.

ACS Style

Diana Lopes; Tânia Melo; Felisa Rey; Elisabete Costa; Ana S.P. Moreira; Maria H. Abreu; Pedro Domingues; Ana I. Lillebø; Ricardo Calado; M. Rosário Domingues. Insights of species-specific polar lipidome signatures of seaweeds fostering their valorization in the blue bioeconomy. Algal Research 2021, 55, 102242 .

AMA Style

Diana Lopes, Tânia Melo, Felisa Rey, Elisabete Costa, Ana S.P. Moreira, Maria H. Abreu, Pedro Domingues, Ana I. Lillebø, Ricardo Calado, M. Rosário Domingues. Insights of species-specific polar lipidome signatures of seaweeds fostering their valorization in the blue bioeconomy. Algal Research. 2021; 55 ():102242.

Chicago/Turabian Style

Diana Lopes; Tânia Melo; Felisa Rey; Elisabete Costa; Ana S.P. Moreira; Maria H. Abreu; Pedro Domingues; Ana I. Lillebø; Ricardo Calado; M. Rosário Domingues. 2021. "Insights of species-specific polar lipidome signatures of seaweeds fostering their valorization in the blue bioeconomy." Algal Research 55, no. : 102242.

Journal article
Published: 23 February 2021 in Scientific Reports
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There is a growing trend to explore microalgae as an alternative resource for the food, feed, pharmaceutical, cosmetic and fuel industry. Moreover, the polar lipidome of microalgae is interesting because of the reports of bioactive polar lipids which could foster new applications for microalgae. In this work, we identified for the first time the Chlorococcum amblystomatis lipidome using hydrophilic interaction liquid chromatography-high resolution electrospray ionization- tandem mass spectrometry (HILIC–HR–ESI–MS/MS). The Chlorococcum amblystomatis strain had a lipid content of 20.77% and the fatty acid profile, determined by gas chromatography-mass spectrometry, has shown that this microalga contains high amounts of omega-3 polyunsaturated fatty acids (PUFAs). The lipidome identified included 245 molecular ions and 350 lipid species comprising 15 different classes of glycolipids (6), phospholipids (7) and betaine lipids (2). Of these, 157 lipid species and the main lipid species of each class were esterified with omega-3 PUFAs. The lipid extract has shown antioxidant activity and anti-inflammatory potential. Lipid extracts also had low values of atherogenic (0.54) and thrombogenic index (0.27). In conclusion, the lipid extracts of Chlorococcum amblystomatis have been found to be a source of lipids rich in omega-3 PUFAs for of great value for the food, feed, cosmetic, nutraceutical and pharmaceutical industries.

ACS Style

Tiago A. Conde; Daniela Couto; Tânia Melo; Margarida Costa; Joana Silva; M. Rosário Domingues; Pedro Domingues. Polar lipidomic profile shows Chlorococcum amblystomatis as a promising source of value-added lipids. Scientific Reports 2021, 11, 1 -23.

AMA Style

Tiago A. Conde, Daniela Couto, Tânia Melo, Margarida Costa, Joana Silva, M. Rosário Domingues, Pedro Domingues. Polar lipidomic profile shows Chlorococcum amblystomatis as a promising source of value-added lipids. Scientific Reports. 2021; 11 (1):1-23.

Chicago/Turabian Style

Tiago A. Conde; Daniela Couto; Tânia Melo; Margarida Costa; Joana Silva; M. Rosário Domingues; Pedro Domingues. 2021. "Polar lipidomic profile shows Chlorococcum amblystomatis as a promising source of value-added lipids." Scientific Reports 11, no. 1: 1-23.

Preprint content
Published: 09 February 2021
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SUMMARY The prevalence of overweight and obesity among children has drastically increased during the last decades and maternal obesity has been demonstrated as one of the ultimate factors. Nutrition-stimulated transgenerational epigenetic regulation of key metabolic genes is fundamental to the developmental origins of the metabolic syndrome. Fetal nutrition may differently influence female and male offspring. In this work, we investigated the sex-dependent programming of maternal obesity in visceral, subcutaneous and brown adipose tissues of offspring using magnetic resonance imaging and spectroscopy and a lipidomic approach combined with a Smart-Seq2 differential sequencing analysis. We show that the triglyceride profile varies between adipose depots, sexes and maternal diet. Our results demonstrate for the first time that a sex-dependent gene programming exists in visceral, subcutaneous and brown adipose tissues. Maternal obesity differentially programs gene expression in adipose depots of female and male offspring, which may contribute to the sex-dependent metabolic complications later in life. Graphical abstract

ACS Style

Christina Savva; Luisa A. Helguero; Marcela González-Granillo; Tânia Melo; Daniela Couto; Byambajav Buyandelger; Sonja Gustafsson; Jianping Liu; Maria Rosário Domingues; Xidan Li; Marion Korach-André. Maternal obesity programs white and brown adipose tissue transcriptome and lipidome in offspring in a sex-dependent manner. 2021, 1 .

AMA Style

Christina Savva, Luisa A. Helguero, Marcela González-Granillo, Tânia Melo, Daniela Couto, Byambajav Buyandelger, Sonja Gustafsson, Jianping Liu, Maria Rosário Domingues, Xidan Li, Marion Korach-André. Maternal obesity programs white and brown adipose tissue transcriptome and lipidome in offspring in a sex-dependent manner. . 2021; ():1.

Chicago/Turabian Style

Christina Savva; Luisa A. Helguero; Marcela González-Granillo; Tânia Melo; Daniela Couto; Byambajav Buyandelger; Sonja Gustafsson; Jianping Liu; Maria Rosário Domingues; Xidan Li; Marion Korach-André. 2021. "Maternal obesity programs white and brown adipose tissue transcriptome and lipidome in offspring in a sex-dependent manner." , no. : 1.

Journal article
Published: 24 January 2021 in Algal Research
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Macroalgae aquaculture is thriving, and kelps such as Saccharina latissima, are now farmed over different production sites across Europe. Macroalgae production in integrated multi-trophic aquaculture setups is gaining relevance due to increased biomass yield and bioremediation potential. Nevertheless, there is a substantiated need to understand how this type of production shapes biomass composition. The present study evaluated the influence of time of harvesting and proximity to nutrient source (fish farm sea cages) on the elemental and biochemical composition of S. latissima, with special emphasis on its lipid content. Overall, the differences recorded for elemental, biochemical and lipid composition of S. latissima occurred consistently with harvesting period rather than with the distance to fish farm sea cages, evidencing that farming kelp in integrated multi-trophic aquaculture setups does not compromise nutritional quality while promoting increased biomass yield. Elemental and biochemical composition differed between kelp biomass harvested in April and those harvested in May and June, with lower C, H and carbohydrates, and higher ash contents in the later. Fatty acid profile analysis revealed an increase in monounsaturated fatty acids along the harvesting period, as well as a decrease in n-3 fatty acids with a concomitant increase of the n-6/n-3 ratio from April to May. Statistical analysis of the polar lipidome identified by liquid chromatography-mass spectrometry revealed differences in specific lipid signatures, displaying a perfect discrimination between harvesting periods. The discrimination between samples from reference and integrated multi-trophic aquaculture sites was only observed for kelp biomass harvested in June. Contributing particularly to these differences are betaine lipids, more abundant in the two later time points, and some lysolipid species, especially abundant in June. These findings will allow a more integrated look at macroalgae production under integrated multi-trophic aquaculture framework, thus promoting the systematization of farming practices that may enhance yield with biochemical quality to match the increasing demands by the food industry and sustainable biorefinary pipeline.

ACS Style

João P. Monteiro; Tânia Melo; Jorunn Skjermo; Silje Forbord; Ole J. Broch; Pedro Domingues; Ricardo Calado; M. Rosário Domingues. Effect of harvesting month and proximity to fish farm sea cages on the lipid profile of cultivated Saccharina latissima. Algal Research 2021, 54, 102201 .

AMA Style

João P. Monteiro, Tânia Melo, Jorunn Skjermo, Silje Forbord, Ole J. Broch, Pedro Domingues, Ricardo Calado, M. Rosário Domingues. Effect of harvesting month and proximity to fish farm sea cages on the lipid profile of cultivated Saccharina latissima. Algal Research. 2021; 54 ():102201.

Chicago/Turabian Style

João P. Monteiro; Tânia Melo; Jorunn Skjermo; Silje Forbord; Ole J. Broch; Pedro Domingues; Ricardo Calado; M. Rosário Domingues. 2021. "Effect of harvesting month and proximity to fish farm sea cages on the lipid profile of cultivated Saccharina latissima." Algal Research 54, no. : 102201.

Review
Published: 02 January 2021 in Antioxidants
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Rheumatoid arthritis (RA) is a highly debilitating chronic inflammatory autoimmune disease most prevalent in women. The true etiology of this disease is complex, multifactorial, and is yet to be completely elucidated. However, oxidative stress and lipid peroxidation are associated with the development and pathogenesis of RA. In this case, oxidative damage biomarkers have been found to be significantly higher in RA patients, associated with the oxidation of biomolecules and the stimulation of inflammatory responses. Lipid peroxidation is one of the major consequences of oxidative stress, with the formation of deleterious lipid hydroperoxides and electrophilic reactive lipid species. Additionally, changes in the lipoprotein profile seem to be common in RA, contributing to cardiovascular diseases and a chronic inflammatory environment. Nevertheless, changes in the lipid profile at a molecular level in RA are still poorly understood. Therefore, the goal of this review was to gather all the information regarding lipid alterations in RA analyzed by mass spectrometry. Studies on the variation of lipid profile in RA using lipidomics showed that fatty acid and phospholipid metabolisms, especially in phosphatidylcholine and phosphatidylethanolamine, are affected in this disease. These promising results could lead to the discovery of new diagnostic lipid biomarkers for early diagnosis of RA and targets for personalized medicine.

ACS Style

Helena Beatriz Ferreira; Tânia Melo; Artur Paiva; Maria Do Rosário Domingues. Insights in the Role of Lipids, Oxidative Stress and Inflammation in Rheumatoid Arthritis Unveiled by New Trends in Lipidomic Investigations. Antioxidants 2021, 10, 45 .

AMA Style

Helena Beatriz Ferreira, Tânia Melo, Artur Paiva, Maria Do Rosário Domingues. Insights in the Role of Lipids, Oxidative Stress and Inflammation in Rheumatoid Arthritis Unveiled by New Trends in Lipidomic Investigations. Antioxidants. 2021; 10 (1):45.

Chicago/Turabian Style

Helena Beatriz Ferreira; Tânia Melo; Artur Paiva; Maria Do Rosário Domingues. 2021. "Insights in the Role of Lipids, Oxidative Stress and Inflammation in Rheumatoid Arthritis Unveiled by New Trends in Lipidomic Investigations." Antioxidants 10, no. 1: 45.

Journal article
Published: 25 November 2020 in Antioxidants
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UV radiation is a well-established environmental risk factor known to cause oxidative stress and disrupt the metabolism of keratinocyte phospholipids. Cannabidiol (CBD) is a phytocannabinoid with anti-inflammatory and antioxidant effects. In this study, we examined changes in the keratinocyte phospholipid profile from nude rat skin exposed to UVA and UVB radiation that was also treated topically with CBD. UVA and UVB radiation promoted up-regulation of phosphatidylcholines (PC), lysophosphatidylcholines (LPC), phosphatidylethanolamines (PE) and down-regulation of sphingomyelin (SM) levels and enhanced the activity of phospholipase A2 (PLA2) and sphingomyelinase (SMase). Application of CBD to the skin of control rats led to down-regulation of SM and up-regulation of SMase activity. After CBD treatment of rats irradiated with UVA or UVB, SM was up-regulated and down-regulated, respectively, while ceramide (CER) levels and SMase activity were down-regulated and up-regulated, respectively. CBD applied to the skin of UV-irradiated rats down-regulated LPC, up-regulated PE and phosphatidylserines (PS) and reduced PLA2 activity. In conclusion, up-regulation of PS may suggest that CBD inhibits their oxidative modification, while changes in the content of PE and SM may indicate a role of CBD in promoting autophagy and improving the status of the transepidermal barrier.

ACS Style

Wojciech Łuczaj; Maria Do Rosário Domingues; Pedro Domingues; Elżbieta Skrzydlewska. Changes in Lipid Profile of Keratinocytes from Rat Skin Exposed to Chronic UVA or UVB Radiation and Topical Application of Cannabidiol. Antioxidants 2020, 9, 1178 .

AMA Style

Wojciech Łuczaj, Maria Do Rosário Domingues, Pedro Domingues, Elżbieta Skrzydlewska. Changes in Lipid Profile of Keratinocytes from Rat Skin Exposed to Chronic UVA or UVB Radiation and Topical Application of Cannabidiol. Antioxidants. 2020; 9 (12):1178.

Chicago/Turabian Style

Wojciech Łuczaj; Maria Do Rosário Domingues; Pedro Domingues; Elżbieta Skrzydlewska. 2020. "Changes in Lipid Profile of Keratinocytes from Rat Skin Exposed to Chronic UVA or UVB Radiation and Topical Application of Cannabidiol." Antioxidants 9, no. 12: 1178.

Journal article
Published: 23 November 2020 in Algal Research
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Chlorella growing under different conditions can adapt its biochemical composition and its fatty acid profile, but the alterations in polar lipids remain poorly explored. In this study, the polar lipidome of Chlorella vulgaris grown under autotrophic (C-Auto) and heterotrophic (C-Hetero) conditions were characterized using hydrophilic interaction liquid chromatography–electrospray ionization mass spectrometry and gas chromatography–mass spectrometry. A total of 173 and 167 lipid species were identified in autotrophic and heterotrophic cultures, respectively, distributed by lyso and phosphatidylcholine, lyso and phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, sulfoquinovosyldiacylglycerol, di- and monogalactosyldiacylglycerol, di- and monogalactosylmonoacylglycerol, ceramide, and inositolphosphoceramide lipid classes. C-Hetero had a higher abundance of low unsaturated species, while C-Auto had a high abundance of highly unsaturated glycolipids and lyso species. The fatty acids profile showed a high content of omega-3 polyunsaturated fatty acids in C-Auto and in omega-6 fatty acids in C-Hetero. The two lipid extracts showed high antioxidant activity and inhibitory capacity for cyclooxygenase-2. This study provides evidence of polar lipid plasticity of microalgae useful for tuning cultivation for biotechnology applications.

ACS Style

Daniela Couto; Tânia Melo; Tiago A. Conde; Margarida Costa; Joana Silva; M. Rosário M. Domingues; Pedro Domingues. Chemoplasticity of the polar lipid profile of the microalgae Chlorella vulgaris grown under heterotrophic and autotrophic conditions. Algal Research 2020, 53, 102128 .

AMA Style

Daniela Couto, Tânia Melo, Tiago A. Conde, Margarida Costa, Joana Silva, M. Rosário M. Domingues, Pedro Domingues. Chemoplasticity of the polar lipid profile of the microalgae Chlorella vulgaris grown under heterotrophic and autotrophic conditions. Algal Research. 2020; 53 ():102128.

Chicago/Turabian Style

Daniela Couto; Tânia Melo; Tiago A. Conde; Margarida Costa; Joana Silva; M. Rosário M. Domingues; Pedro Domingues. 2020. "Chemoplasticity of the polar lipid profile of the microalgae Chlorella vulgaris grown under heterotrophic and autotrophic conditions." Algal Research 53, no. : 102128.

Journal article
Published: 12 November 2020 in Archives of Biochemistry and Biophysics
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Multiple sclerosis is a neurodegenerative disease causing disability in young adults. Alterations in metabolism and lipid profile have been associated with this disease. Several studies have reported changes in the metabolism of arachidonic acid and the profile of fatty acids, ceramides, phospholipids and lipid peroxidation products. Nevertheless, the understanding of the modulation of circulating lipids at the molecular level in multiple sclerosis remains unclear. In the present study, we sought to assess the existence of a distinctive lipid signature of multiple sclerosis using an untargeted lipidomics approach. It also aimed to assess the differences in lipid profile between disease status (relapse and remission). For this, we used hydrophilic interaction liquid chromatography coupled with mass spectrometry for phospholipidomic profiling of serum samples from patients with multiple sclerosis. Our results demonstrated that multiple sclerosis has a phospholipidomic signature different from that of healthy controls, especially the PE, PC, LPE, ether-linked PE and ether-linked PC species. Plasmalogen PC and PE species, which are natural endogenous antioxidants, as well as PC and PE polyunsaturated fatty acid esterified species showed significantly lower levels in patients with multiple sclerosis and patients in both remission and relapse of multiple sclerosis. Our results show for the first time that the serum phospholipidome of multiple sclerosis is significantly different from that of healthy controls and that few phospholipids, with the lowest p-value, such as PC(34:3), PC(36:6), PE(40:10) and PC(38:1) may be suitable as biomarkers for clinical applications in multiple sclerosis.

ACS Style

Helena Beatriz Ferreira; Tânia Melo; Andreia Monteiro; Artur Paiva; Pedro Domingues; M. Rosário Domingues. Serum phospholipidomics reveals altered lipid profile and promising biomarkers in multiple sclerosis. Archives of Biochemistry and Biophysics 2020, 697, 108672 .

AMA Style

Helena Beatriz Ferreira, Tânia Melo, Andreia Monteiro, Artur Paiva, Pedro Domingues, M. Rosário Domingues. Serum phospholipidomics reveals altered lipid profile and promising biomarkers in multiple sclerosis. Archives of Biochemistry and Biophysics. 2020; 697 ():108672.

Chicago/Turabian Style

Helena Beatriz Ferreira; Tânia Melo; Andreia Monteiro; Artur Paiva; Pedro Domingues; M. Rosário Domingues. 2020. "Serum phospholipidomics reveals altered lipid profile and promising biomarkers in multiple sclerosis." Archives of Biochemistry and Biophysics 697, no. : 108672.