Objective: to analyze the acute effect of concurrent training on hematological and hepatic profile, and muscle injury markers in trained individuals. Methodology: 11 trained males (27.0 ± 1.13 years old) were undergone to body composition, muscle strength, cardiorespiratory fitness evaluation and a concurrent training (CT) session. Blood samples of glucose (GLU), total cholesterol (TC), triglycerides (TG), HDL-cholesterol fraction (HDL), LDL-cholesterol fraction (LDL), gamma glutamyl transferase (GGT), alanine amino transferase (ALT), aspartate amino transferase (AST), urea (Ur), bilirubin (Bil), total protein (TP), lactate (LAC), Creatine kinase (CPK) and lactate dehydrogenase (LDH) were collected before and immediately after the CT. Results: paired Student’s t-test showed a significant increase on HDL (p=0.037), GGT (0.015), AST (p=0.0001), Ur (p=0.002), Bil (p=0.0001), TP (p=0.024), LAC (p=0.015), CPK (p=0.0001) and LHD (p=0.005) levels. Conclusions: A single session of concurrent training increased the blood concentration of the biochemical parameters studied related to cardiometabolic health in the evaluated individuals. Furthermore, it seems that the individuals' fitness level may influence the behavior of biochemical variables in response to physical exercise.