In terrestrial plants, strigolactones act as multifunctional endo- and exo-signals. On microalgae, the strigolactones determine akin effects: induce symbiosis formation with fungi and bacteria and enhance photosynthesis efficiency and accumulation of biomass. This work aims to synthesize and identify strigolactone mimics that promote photosynthesis and biomass accumulation in microalgae with biotechnological potential. Novel strigolactone mimics easily accessible in significant amounts were prepared and fully characterized. The first two novel compounds contain 3,5-disubstituted aryloxy moieties connected to the bioactive furan-2-one ring. In the second group of compounds, a benzothiazole ring is connected directly through the cyclic nitrogen atom to the bioactive furan-2-one ring. The novel strigolactone mimics were tested on Chlorella sorokiniana NIVA-CHL 176. All tested strigolactones increased the accumulation of chlorophyll b in microalgae biomass. The SL-F3 mimic, 3-(4-methyl-5-oxo-2,5-dihydrofuran-2-yl)-3H-benzothiazol-2-one (7), proved the most efficient. This compound, applied at a concentration of 10⁻⁷ M, determined a significant biomass accumulation, higher by more than 15% compared to untreated control, and improved the quantum yield efficiency of photosystem II. SL-F2 mimic, 5-(3,5-dibromophenoxy)-3-methyl-5H-furan-2-one (4), applied at a concentration of 10⁻⁹ M, improved protein production and slightly stimulated biomass accumulation. Potential utilization of the new strigolactone mimics as microalgae biostimulants is discussed.

This study aimed to evaluate the impact of a more cost-efficient strigolactone mimic SL-6 on Chlorella sorokiniana NIVA-CHL 176 growth in comparison with the strigolactone analog GR24 and the plant biostimulant functions of microalgal extracts. Three molar SL-6 concentrations were tested: 10⁻⁷ M, 10⁻⁸ M, and 10⁻⁹ M, respectively. Five parameters of microalgal growth were assessed: optical density, turbidity, biomass production, chlorophyll fluorescence, and pigment concentration. Results after 15 days of culturing revealed that the SL-6 treatments significantly enhanced biomass production (13.53% at 10⁻⁹ M), pigment synthesis, and photosystem II activity (14.38% at 10⁻⁹ M). The highest increases in pigments induced by SL-6 were 15.7% for chlorophyll a (at 10⁻⁸ M SL-6), 12.87% for chlorophyll b (at 10⁻⁹ M SL-6), 2.3% for carotenoids (at 10⁻⁸ M SL-6), and 10.78% for total pigments (at 10⁻⁸ M SL-6) per gram biomass compared to the solvent control (DMSO). Higher doses of GR24 and SL-6 (10⁻⁷ M) inhibited microalgal growth, reducing cell density, biomass production, and pigment synthesis. The microalgal extracts acted as plant biostimulants, stimulating root and shoot elongation and proton pump functioning of mung seedlings in the presence and absence of salt stress. The extracts from SL-6 biostimulated C. sorokiniana were more active as plant biostimulants than the extracts from the non-stimulated C. sorokiniana.

Strigolactones (SLs) have potential to be used in sustainable agriculture to mitigate various stresses that plants have to deal with. The natural SLs, as well as the synthetic analogs, are difficult to obtain in sufficient amounts for practical applications. At the same time, fluorescent SLs would be useful for the mechanistic understanding of their effects based on bio-imaging or spectroscopic techniques. In this study, new fluorescent SL mimics containing a substituted 1,8-naphthalimide ring system connected through an ether link to a bioactive furan-2-one moiety were prepared. The structural, spectroscopic, and biological activity of the new SL mimics on phytopathogens were investigated and compared with previously synthetized fluorescent SL mimics. The chemical group at the C-6 position of the naphthalimide ring influences the fluorescence parameters. All SL mimics showed effects similar to GR24 on phytopathogens, indicating their suitability for practical applications. The pattern of the biological activity depended on the fungal species, SL mimic and concentration, and hyphal order. This dependence is probably related to the specificity of each fungal receptor–SL mimic interaction, which will have to be analyzed in-depth. Based on the biological properties and spectroscopic particularities, one SL mimic could be a good candidate for microscopic and spectroscopic investigations.

This study aimed to evaluate the impact on Chlorella sorokiniana NIVA-CHL 176 growth and the plant biostimulant functions of the microalgal extract of the strigolactone mimic SL-6, in com-parison with the strigolactone analog GR24. Three molar SL-6 concentrations were tested: 10-7 M, 10-8 M, and 10-9 M, respectively. Five parameters of the microalgal growth were assessed: optical density, turbidity, biomass production, chlorophyll fluorescence, and pigment concentration. Results after 15 days of culturing revealed that the SL-6 treatments significantly enhanced bio-mass production, chlorophyll synthesis, and photosystem II activity. Treatment with SL-6 c3 (10-9 M) exhibited significant increases in chlorophyll a (27.55%), chlorophyll b (24.81%), carotenoids (12.31%), and total pigments (24.00%) compared to the solvent control (DMSO). Experimental treatment SL-6 c2 (10-8 M) followed next and recorded notable enhancements in chlorophyll a (25.80%), carotenoids (9.77%), and total pigments (20.08%). Conversely, higher doses of GR24 and SL-6 (10-7 M) inhibited the microalgal growth, reducing cell density, biomass production, and pigment synthesis. The microalgal extracts acted as a plant biostimulant stimulating root and shoot elongation and proton pump functioning in the presence and absence of salt stress. The extracts from SL-6 bio-stimulated C. sorokiniana NIVA-CHL 176 are more active as plant biostimulants.