Introduction: The repair capability of traumatized articular cartilage is highly limited so that joint injuries often lead to osteoarthritis. Migratory chondrogenic progenitor cells (CPC) might represent a target cell population for in situ regeneration. This study aims to clarify, whether 1) CPC are present in regions of macroscopically intact cartilage from human osteoarthritic joints, 2) CPC migration is stimulated by single growth factors and the cocktail of factors released from traumatized cartilage and 3) CPC migration is influenced by cytokines present in traumatized joints. Methods: We characterized the cells growing out from macroscopically intact human osteoarthritic cartilage using a panel of positive and negative surface markers and analyzed their differentiation capacity. The migratory response to platelet-derived growth factor (PDGF)-BB, insulin-like growth factor 1 (IGF-1), supernatants obtained from in vitro traumatized cartilage and interleukin-1 beta (IL-1β) as well as tumor necrosis factor alpha (TNF-α) were tested with a modified Boyden chamber assay. The influence of IL-1β and TNF-α was additionally examined by scratch assays and outgrowth experiments. Results: A comparison of 25 quadruplicate marker combinations in CPC and bone-marrow derived mesenchymal stromal cells showed a similar expression profile. CPC cultures had the potential for adipogenic, osteogenic and chondrogenic differentiation. PDGF-BB and IGF-1, such as the supernatant from traumatized cartilage, induced a significant site-directed migratory response. IL-1β and TNF-α significantly reduced basal cell migration and abrogated the stimulative effect of the growth factors and the trauma supernatant. Both cytokines also inhibited cell migration in the scratch assay and primary outgrowth of CPC from cartilage tissue. In contrast, the cytokine IL-6, which is present in trauma supernatant, did not affect growth factor induced migration of CPC. Conclusion: These results indicate that traumatized cartilage releases chemoattractive factors for CPC but IL-1β and TNF-α inhibit their migratory activity which might contribute to the low regenerative potential of cartilage in vivo.

Short stems have gained popularity in recent years. Because of encouraging clinical results, indications have been expended from young to elderly and obese patients. However, long-term results are lacking. The purpose of this study was to evaluate the influence of gender, age, body weight, body mass index (BMI), and offset version on short-stem migration in correlation to the clinical outcome. The implant migration of 202 metaphyseal-anchoring, calcar-guided short stems in 151 patients was assessed by “Einzel-Bild-Roentgen-Analyse” femoral component analysis (EBRA-FCA, femoral component analysis) in a 2-year follow-up. Full weight bearing was allowed directly after surgery. Patients were divided into groups regarding gender, age, body weight, BMI, and offset version. The Harris hip score (HHS) and satisfaction on visual analogue scale (VAS) were analyzed. After 2 years, mean axial subsidence of all 202 implants was 1.43 mm (standard deviation, SD 1.45 mm). A continuous reduction of initially pronounced subsidence over time could be observed. None of the stems had to be revised. Statistically significant increased rates of subsidence were seen in male (1.68 mm; SD 1.56 mm; p = 0.005) and heavy patients (1.54 mm; SD 1.48 mm; p = 0.022). No differences in implant migration were found regarding age, BMI, and different offset versions. HHS improved markedly from 45.8 (SD 15.9) to 98.1 (SD 4.7) while satisfaction on VAS improved from 1.8 (SD 2.2) to 9.7 (SD 0.9) after 2 years. The results suggest a migration pattern with initially pronounced subsidence followed by subsequent stabilization. Male and obese patients show a slightly increased initial subsidence without any signs of sustained micromovement. No correlation was found concerning clinical results and pronounced initial subsidence above the threshold of 1.5 mm. No aseptic loosening or other signs of implant failure were seen within the observation period of 2 years. German Clinical Trials Register, DRKS00009834 .

Background: Bony Bankart lesions larger than a certain size can lead to a high redislocation rate, despite treatment with Bankart repair. Detection and measurement of glenoid bone loss play key roles in selecting the appropriate surgical therapy in patients with shoulder instability. There is controversy about which diagnostic modalities, using different measurement methods, provide the best diagnostic validity. Questions/purposes: (1) What are the diagnostic accuracies of true AP radiographs, West Point (WP) view radiographs, MRI, and CT to detect glenoid bone loss? (2) Are there differences in the measurements of glenoid bone loss on MRI and CT? (3) What are the intrarater and interrater reliabilities of CT and MRI to measure glenoid bone loss? Methods: Between August 2012 and February 2017, we treated 80 patients for anterior shoulder instability. Of those, we considered patients with available preoperative true AP radiographs, WP radiographs, CT images, and MR images of the affected shoulder as potentially eligible. Based on that, 63% (50 of 80) of patients were eligible for analysis; 31% (25 of 80) were excluded because not all planes or slices (such as sagittal, axial, or frontal) of each diagnostic imaging modalities were available and 7% (5 of 80) because of the insufficient quality of diagnostic images (for example, setting of the layers did not allow adequate en face view of the glenoid). Preoperative true AP radiographs, WP radiographs, CT images and MR images of the affected shoulders were retrospectively assessed for the presence of glenoid bone loss by two blinded observers at a median (range) 25 months (12 to 66) postoperatively. To evaluate sensitivity, specificity, positive predictive value, negative predictive value, accuracy, diagnostic odds ratio, positive likelihood ratio, negative likelihood ratio, and area under the curve (AUC), we compared the detection of glenoid bone loss at follow-up achieved with the aforementioned imaging modalities with intraoperative arthroscopic detection. In all patients with glenoid bone loss, two blinded observers measured the size of the glenoid bone loss on preoperative CT and MR images using six measuring techniques: depth and length of the glenoid bone loss, Bigliani classification, best-fit circle width loss method, AP distance method, surface area method, and Gerber X ratio. Subsequently, the sizes of the glenoid bone loss determined using CT and MRI were compared. To estimate intraobserver and interobserver reliability, measurements were performed in a blinded fashion by two observers. Their level of experience was equivalent to that of orthopaedic residents, and they completed a training protocol before the measurements. Results: For the ability to accurately diagnose Bankart lesions, the AUC (accuracy of a diagnostic test; the closer to 1.0, the more accurate the test) was good for MRI (0.83 [95% confidence interval 0.70 to 0.94]; p < 0.01), fair for CT (0.79 [95% CI 0.66 to 0.92]; p < 0.01), poor for WP radiographs (0.69 [95% CI 0.54 to 0.85]; p = 0.02) and failed for true AP radiographs (0.55 [95% CI 0.39 to 0.72]; p = 0.69). In paired comparisons, there were no differences between CT and MRI regarding (median [range]) lesion width (2.33 mm [0.35 to 4.53] versus 2.26 mm [0.90 to 3.47], p = 0.71) and depth (0.42 mm [0.80 to 1.39] versus 0.40 mm [0.06 to 1.17]; p = 0.54), and there were no differences concerning the other measurement methods: best-fit circle width loss method (15.02% [2.48% to 41.59%] versus 13.38% [2.00% to 36.34%]; p = 0.66), AP distances method (15.48% [1.44% to 42.01%] versus 12.88% [1.43% to 36.34%]; p = 0.63), surface area method (14.01% [0.87% to 38.25] versus 11.72% [2.45% to 37.97%]; p = 0.68), and Gerber X ratio (0.75 [0.13 to 1.47] versus 0.76 [0.27 to 1.13]; p = 0.41). Except for the moderate interrater reliability of the Bigliani classification using CT (intraclass correlation coefficient = 0.599 [95% CI 0.246 to 0.834]; p = 0.03) and acceptable interrater reliability of the Gerber X ratio using CT (0.775 [95% CI 0.542 to 0.899]; p < 0.01), all other measurement methods had good or excellent intrarater and interrater reliabilities on MRI and CT. Conclusion: The results of this study show that CT and MRI can accurately detect glenoid bone loss, whereas WP radiographs can only recognize them poorly, and true AP radiographs do not provide any adequate diagnostic accuracy. In addition, when measuring glenoid bone loss, MRI images of the analyzed measurement methods yielded sizes that were no different from CT measurements. Finally, the use of MRI images to measure Bankart bone lesions gave good-to-excellent reliability in the present study, which was not inferior to CT findings. Considering the advantages including lower radiation exposure and the ability to assess the condition of the labrum using MRI, we believe MRI can help surgeons avoid ordering additional CT imaging in clinical practice for the diagnosis of anterior shoulder instability in patients with glenoid bone loss. Future studies should investigate the reproducibility of our results with a larger number of patients, using other measurement methods that include examination of the opposite side or with three-dimensional reconstructions. Level of Evidence: Level I diagnostic study.

Short-stem total hip arthroplasty (THA) potentially offers advantages compared to conventional THA, including sparing bone and soft tissue and being a facilitated and less traumatic implantation. However, the indication is limited to patients with sufficient bone quality. Cemented short-stem THA might provide an alternative to conventional cemented THA. To date, no cemented short stem is available on the market. In the present in vitro study, primary stability of a new cemented short stem was evaluated, comparing standard (undersized stem) versus line-to-line (same-sized stem) cementing techniques, using six pairs of human cadaver femurs. Primary stability, including reversible micromotion and irreversible migration, was assessed in a dynamic material-testing machine. Fracture load was tested and fracture pattern analyzed. Both cementation techniques (standard vs. line-to-line) displayed comparable results with respect to primary stability without any statistical differences (micromotion: 17.5 μm vs. 9.6 μm (p = 0.063); migration: 9.5 μm vs. 38.2 μm (p = 0.188)). Regarding fracture load, again, no difference was observed (3670 N vs. 3687 N (p = 0.063)). In all cases, proximal fractures of Vancouver type B3 occurred. The present in vitro study demonstrates that the line-to-line cementation technique, which is favourable regarding the philosophy of short stem THA, can be further pursued in the course of the development of a cemented short stem. Further investigations should address how well the cemented short stem compares to well-established cemented straight-stem designs.

Introduction: In hip preservation surgery, the term “borderline hip dysplasia” was used when the lateral center edge angle (LCEA), historically described by Wiberg, measured 18–25°. In recent years, several radiographic parameters have been described to assess the antero posterior coverage of the femoral head, for example, the anterior and posterior wall index (AWI and PWI). This allowed an increasingly comprehensive understanding of acetabular morphology and a questioning of the borderline definition. Material and methods: A retrospective review of 397 consecutive hips was performed, all treated with triple pelvic osteotomy (TPO) due to symptomatic hip dysplasia. On all preoperative pelvic radiographs with a LCEA of 18–25°, acetabular index (AI), AWI and PWI were measured. With these values, the hips were categorized into laterally, antero-laterally and postero-laterally dysplastic and stratified by gender. Intra- and interobserver correlation of the parameters was analyzed by intraclass correlation coefficient (ICC). Results: According to LCEA, 192 hips were identified as “borderline dysplastic”. Based on AWI and PWI, the categorization resulted in 116 laterally dysplastic (60.4%), 33 antero-laterally (17.2%) and 43 postero-laterally dysplastic hips (22.4%). Gender stratification revealed that male acetabula seemed to be slightly more postero-laterally deficient than female (mean PWI 0.80 vs 0.89; p = 0.017). ICC confirmed highly accurate and reproducible readings of all parameters. Conclusion: The rather high proportion of symptomatic hips labelled borderline dysplastic suggested, that there might be substantial acetabular deficiency not recognizable by LCEA. Comprehensive deformity analysis using LCEA, AI, AWI and PWI showed, that 40% of these hips were deficient either antero-laterally or postero-laterally. Male acetabula were more deficient postero-laterally than female.

Background: Retearing of the supraspinatus (SSP) tendon after repair is relatively common, but its cause is rarely clear. Although the role of acromion morphology and glenoid orientation in the pathogenesis of primary SSP tendon tears have frequently been analyzed, their association with the risk of rerupture of a repaired SSP tendon is poorly understood. Questions/purposes: (1) Is acromial morphology associated with the risk of retear after SSP tendon repair? (2) Is there an association between inclination and version of the glenoid and the odds for retear of the SSP tendon after repair? (3) Are there differences in outcome scores between patients who had intact cuff repairs and those who had retears? Methods: Between August 2012 and December 2015, we treated 92 patients for SSP tendon tears; all of these patients were considered for inclusion in the present study. We considered patients with complete tear of the SSP that was reconstructed with a double-row repair and a minimum follow-up of 2 years as potentially eligible. Based on these criteria, 28% (26 of 92) were excluded because they had a partial rupture and did not receive a double-row reconstruction. A further 9% (eight of 92) were excluded because of missing planes or slices (such as sagittal, axial, or frontal) on MRI, and another 3% (three of 92) were lost before the minimum study follow-up interval or had incomplete datasets, leaving 60% (55 of 92) for inclusion in the present analysis. All included patients had a minimum follow-up of 2 years; follow-up with MRI occurred at a mean duration of 2.3 ± 0.4 years postoperatively. All patients were asked to complete the Western Ontario Rotator Cuff Index and Oxford Shoulder Scores, and they underwent MRI of the operated-on shoulder. Preoperative true AP radiographs and MR images of the affected shoulders were retrospectively assessed by measuring the acromiohumeral interval, critical shoulder angle, acromial slope, acromial tilt, acromial index, lateral acromial angle, and glenoid version and inclination. The patients also underwent acromioplasty, in which the underface of the acromion was flattened. To rule out any change in the above parameters because of acromioplasty, these parameters were compared using preoperative and postoperative MR images and showed no difference. In addition, the tendon integrity and quality on postoperative MRI were analyzed independently of one another by the same two observers using the Sugaya and Castricini classifications, accounting for atrophy and fatty degeneration of the SSP muscle. To assess interobserver reliability, the two observers took measurements independently from each other. They were orthopaedic residents who completed a training session before taking the measurements. All measurements had excellent intrarater (Cronbach alpha 0.996 [95% confidence interval (CI) 0.99 to 1.00; p > 0.01) and interrater (interrater correlation coefficient 0.975 [95% CI 0.97 to 0.98]; p > 0.01) reliabilities. To answer the study’s first question, SSP integrity on postoperative MRI was compared with acromial morphologic parameters measured on preoperative AP radiographs and MR images. To answer the second question, the postoperative integrity and quality of the SSP tendon were correlated with glenoid inclination and glenoid version. To answer our third question, we compared outcome scores between patients with intact SSP tendons and those with reruptured SSP tendons. To investigate any correlation among the acromial morphology, glenoid orientation, and postoperative outcomes, a binomial logarithmic regression analysis was performed. Receiver operating characteristic curves were used to determine cutoff points for the radiologic parameters that showed a correlation in the binomial regression analysis. Results: After controlling for potentially confounding variables such as acromioplasty or preoperative fatty infiltration as well as muscle atrophy, the only morphological parameters associated with a higher risk (adjusted odds ratio) of SSP tendon rerupture were the acromiohumeral interval (adjusted OR 0.9 [95% CI 0.9 to 0.99]; p < 0.01) and acromial slope (adjusted OR 1.4 [95% CI 1.1 to 1.8]; p < 0.01). The critical shoulder angle, acromial tilt, acromial index, and lateral acromial angle were not associated with the risk of rerupture. The cutoff values for acromial slope and acromiohumeral interval were 24.5° and 7.4 mm, respectively. Patients with an acromiohumeral interval smaller than 7.4 mm or an acromial slope greater than 24.5° had higher odds (acromiohumeral interval: OR 11 [95% CI 2 to 46]; p = 0.01 and acromial slope: OR 9 [95% CI 2 to 46]; p = 0.04) for rerupture of the SSP. No difference was found between patients with intact SSP tendons and those with reruptured SSP tendons in terms of glenoid inclination (6° ± 4° versus 6° ± 3°, mean difference 0.8° [-1° to 3°]; p < 0.48) and glenoid version (-2° ± 3° versus -3° ± 3°, mean difference 1° [-1° to 3°]; p < 0.30). No difference was found between the intact and reruptured SSP groups regarding clinical outcomes (Western Ontario Rotator Cuff Index: 98 ± 2 versus 97 ± 3, mean difference 0.73 [95% CI -0.30 to 0.31]; p = 0.96; Oxford Shoulder Score: 26 ± 13 versus 23 ± 10, mean difference 2.80 [95% CI -4.12 to 9.72]; p = 0.41). Conclusion: The preoperative acromiohumeral interval and acromial slope are associated with SSP tendon rerupture after repair. Conversely, the critical shoulder angle, acromial tilt, lateral acromial angle, and acromial index had no association with the postoperative outcome. Additionally, glenoid inclination and version were not associated with the rerupture rate after SSP tendon repair. A detailed analysis of the acromiohumeral interval and acromial slope is recommended in clinical practice in patients undergoing SSP tendon...