Postharvest leaf senescence is a pivotal determinant influencing the quality and shelf life of leafy vegetables, exemplified by pak choi (Brassica rapa L. subsp. chinensis). While the regulatory role of gibberellin (GA) in modulating leaf senescence has been documented across diverse plant species, the underlying physiological and molecular mechanisms remain insufficiently characterized. This study, through a combination of transcriptomic and metabolomic analyses, investigated the effect of exogenous GA on postharvest leaf senescence in pak choi. GA treatment alleviated etiolation, maintained chlorophyll levels, reduced conductivity and malondialdehyde content, and delayed the onset of senescence symptoms in postharvest pak choi. Transcriptome profiling indicated that GA suppressed the expression of the senescence-associated genes BraSRGs and BraSAGs. In addition, GA influenced chlorophyll degradation and preserved chlorophyll content by modulating the expression of genes implicated in chlorophyll metabolism, including BraPPH, BraSGR1, BraNYCI, and BraPAO. GA treatment impacted lipid levels and regulated the degradation of membrane phospholipids. Furthermore, exogenous GA treatment disrupted the efficacy of the jasmonic acid signal pathway, primarily through the transcriptional downregulation of key regulatory genes, including BraJAZ10 and BraJAR1. These results provide insights into the role of GA in delaying postharvest leaf senescence and highlight potential targets for improving postharvest management in leafy vegetables.

Calmodulin (CaM) and calmodulin-like proteins (CMLs) are crucial for calcium signal transduction in plants. Although CaM/CML genes have been extensively studied in various plant species, research on these genes in Brassica oleracea is still limited. In this study, 14 BoCaM and 75 BoCML genes were identified in the B. oleracea genome through a genome-wide search. Phylogenetic analysis categorized these genes, along with their homologs in Arabidopsis and rice, into six distinct groups. All BoCaM/BoCML genes were unevenly distributed across the nine chromosomes of B. oleracea, with 52 of them lacking introns. Collinearity analysis revealed that CaM/CML genes in Arabidopsis are present in multiple copies in the B. oleracea genome. Moreover, the majority of BoCaM/BoCML genes exhibited distinct expression patterns across the different tissues, indicating their role in the growth and development of B. oleracea. A clustering heatmap of BoCaM/BoCML gene expression showed distinct patterns before and four days after Hyaloperonospora parasitica infection, dividing the genes into five groups based on their expression patterns. Notably, BoCML46-2 is significantly downregulated in both susceptible and resistant materials, suggesting that it plays an important role in responding to H. parasitica infection. This study conducted a comprehensive survey of the BoCaM/BoCML gene family in B. oleracea. It could serve as a theoretical foundation for further functional identification and utilization of family members and their role in the interaction between B. oleracea and H. parasitica.

Choy Sum (Brassica rapa subsp. chinensis var. parachinensis), also known as flowering Chinese cabbage, is an important stalk vegetable in Asia. However, the unique regulatory mechanism governing its “easy-bolting yet susceptible to premature bolting” trait remains poorly understood. The phosphatidyl ethanolamine-binding protein (PEBP) family serves as a central regulator of bolting, flowering, and growth development in plants. But this gene family has not been systematically identified and studied in Choy Sum yet. Therefore, this study systematically identified and analyzed the members of the PEBP gene family in Choy Sum using bioinformatics, transcriptomics, real-time fluorescence quantification, subcellular localization, and transgenic techniques. A total of 12 BcPEBP genes were identified and categorized into three subfamilies: FT-like, TFL1-like, and MFT-like. Phylogenomic analyses revealed family expansion through whole-genome duplication with strong purifying selection. Most members have highly conserved core motifs and gene structures. Protein sequence alignment showed that BcFT-2 and BcTFL-2 underwent non-synonymous mutations at key residues. The analysis of cis-acting elements suggests that the BcPEBP gene may be influenced by complex hormone and light regulatory networks. Expression profiling demonstrated leaf-specific upregulation of BcFT-1/2 during development and shoot apices-predominant expression of BcTFL1 genes, and the expression between homologous genes of BcTFL1-1/3 is more refined. Subcellular localization confirmed dual nuclear and plasma membrane targeting of BcFT-1/2 proteins. Overexpression of BcFT-1/2 in transgenic Arabidopsis promotes flowering. These findings establish BcPEBP genes as key bolting regulators and provide molecular targets for breeding-improved varieties.