Introduction: The ongoing concern of the medical profession regarding chronic medication is related to increasing patient adherence and compliance to treatment and reducing medication side effects. In this respect, drugs represented by fixed-dose combinations of active substances within the same tablet have emerged. Such a principle can be extrapolated by following the potential beneficial effects that a chronic medication can have on chronic pathologies affecting different systems. Materials and Methods: The study included 48 female Albino Wistar rats, aged 16–18 months, which were divided into two groups: ovariectomized and non-ovariectomized rats. One batch of 12 non-ovariectomized rats received no treatment, becoming a control batch (NOVX-M). The ovariectomized (OVX) group was divided into 3 batches of 12 rats each: no treatment, control (OVX-M), fenofibrate-treated (OVX-F) and statin-treated (OVX-S) rats. At 12 weeks after ovariectomy, a femoral fracture occurred in the right hind limb of all animals included in the experiment To reveal the changes, at intervals of 2, 4, 6 and 8 weeks post-fracture, the proximal part of the femur was evaluated by NMR diffusiometry, which allows random motion of proton molecules expressed by self-diffusion coefficients, D, thus allowing analysis of the size and complexity of microscopic order cavities within biological structures, such as pores inside bones. Results: The effects of hypolipidemic medication in the absence of estrogen were evidenced, proving the beneficial effect that fenofibrate can have in preserving healthy tissue exposed to osteoporotic risk during the menopausal period. The effects of lipid-lowering medication are also influenced by the duration of administration. Conclusions: Osteoporosis and heart disease are two chronic pathologies that affect mainly female population in the second half of life, and proving the dual therapeutic potential of lipid-lowering medication may also have positive effects by increasing adherence and compliance to treatment.

This study aims to evaluate the knowledge and consumption of healthy foods, respectively, of oils as sources of omega-3 and dietary supplements with omega-3, among a population of young people in the center of Romania. With the objectives of identifying the factors that can influence the consumption of healthy fats and the orientation toward actions to promote less known food resources, in order to diversify the healthy food intake, the long-term improvement of the health-related effects of food. One of the most important aspects of health is nutrition and its role in reducing the incidence of chronic diseases is supported by scientific data. In this research, the authors analyzed the level of information and the factors determining food choices with particular reference to the consumption of healthy fats and/or supplements from these fats, in order to highlight the behavior of individuals in relation to food. For this purpose, a questionnaire about food and healthy fats (omega-3) consumption and frequency was applied to the food groups of interest, along with other factors pertaining to lifestyle. The questionnaire distributed online mainly included questions related to the consumption of fats and the respondents' knowledge about them. The interest in participating in the study was manifested mainly in the young age segment. The collected data were analyzed statistically was done through Graph Pad Prism ver. 9 software with the establishment of a statistical significance threshold of 0.05. There is a certain degree of superficiality in the knowledge of the importance and use of foods rich in omega-3. The statistically significant association has been identified both between age and the rules established in the family for observing the schedule of meals and between age and benefiting from an evaluation of the eating behavior. Statistically significant association has also been observed between the level of education and the knowledge on the health benefits of vegetable oils. The statistically significant association was also present between the level of education and the respondents' appreciation of the essential role of eating behavior in disease prevention. Identifying the consumer profile in relation to their attitude toward healthy foods, especially fats, in order to adapt nutritional interventions with the aim to promote healthy food choices that have an impact on the health of the individual and also of the population.

Introduction. The benefits or risks of egg consumption have been a challenge for researchers in identifying the most effective methods for evaluating micronutrients and, implicitly, the nutritional properties of eggs. The purpose of this work was to highlight the comparative nutritional properties of eggs from hens with similar productive yields through innovative methods, such as FT-IR spectra. Methods. This paper aimed to determine the omega 3 fatty acid (O3FA) content in eggs. In this study were used eggs from four different breeds of laying hens: Lohmann Brown, Isa Brown, Australorp and a hybrid breed. The eggs were collected daily, and their evaluation was carried out at an interval of 24 h after collection. For the calibration of the measurements, the Omacor product whose omega 3 content was evaluated by the National Agency of Medicines and Medical Devices from Romania was used as a reference standard. Results. The study supports that there is a significant difference in the polyunsaturated FA and saturated FA content between eggs of various hen breeds. Conclusions. The composition of egg contents differs in terms of micronutrients both at the level of the egg white, yolk and shell, depending on the breed of the laying hen. This experiment can support the idea that eggs with a lower atherogenic potential can be recommended based on the chicken breed.

Background and Objectives: BRAF p.V600E status has diagnostic, prognostic, and therapeutic relevance in colorectal carcinoma. Molecular assays remain the reference methods, whereas mutation-specific BRAF (VE1 clone) immunohistochemistry offers a rapid tissue-based alternative. We evaluated VE1–molecular concordance and factors affecting diagnostic performance. Materials and Methods: PubMed/MEDLINE, Scopus, and Web of Science were searched in August 2025 and updated on 27 July 2026 for English-language original studies published from 2014 through the update date. Eligible reports applied VE1 and a recognized molecular reference method to the same colorectal carcinoma specimens or a defined overlapping cohort. Study selection and data extraction were performed by one reviewer and independently verified by a second reviewer. Risk of bias and applicability were assessed at the estimate level with QUADAS-3. Because of substantial clinical and methodological heterogeneity, no meta-analysis was performed, and the findings were synthesized descriptively. Results: Of 704 database records, 358 unique records were screened; 38 full-text reports were assessed and 28 were included. Twenty-three reports or defined subcohorts provided complete or clearly reconstructible 2 × 2 comparisons. Sensitivity ranged from 74.6% to 100% and specificity from 66.7% to 100%. Most optimized protocols exceeded 90% for both measures, whereas selected sampling, weak or focal staining, post hoc thresholds, protocol effects, and selective molecular verification reduced reliability. Conclusions: VE1 immunohistochemistry may provide a rapid, locally validated triage method for detecting BRAF p.V600E in colorectal carcinoma. However, substantial heterogeneity and recurrent risk-of-bias concerns limit the generalizability of the available estimates and do not support universal substitution for molecular testing. Equivocal, technically inadequate, or clinically discordant results require molecular clarification.

Introduction: The ongoing concern of the medical profession regarding chronic medication is related to increasing patient adherence and compliance to treatment and reducing medication side effects. In this respect, drugs represented by fixed-dose combinations of active substances within the same tablet have emerged. Such a principle can be extrapolated by following the potential beneficial effects that a chronic medication can have on chronic pathologies affecting different systems. Material and method: The study included 48 female Albino Wistar rats, aged 16-18 months, which were divided into two groups. One group of 12 non-ovariectomized rats received no treatment, becoming a control group (NOVX-M). The ovariectomized (OVX) group was divided into 3 groups of 12 rats: no treatment, control (OVX-M), fenofibrate-treated (OVX-F) and statin-treated (OVX-S) rats. To reveal the changes, at intervals of 2, 4, 6, 8 weeks the proximal part of the femur was evaluated by NMR diffusiometry. Results: The effects of hypolipidemic medication in the absence of estrogen were evidenced, proving the beneficial effect that fenofibrate can have in preserving healthy tissue exposed to osteoporotic risk during the menopausal period. The effects of lipid-lowering medication are also influenced by the duration of administration. Conclusions: Osteoporosis and heart disease are two chronic pathologies that affect mainly female population in the second half of life, and proving the dual therapeutic potential of lipid-lowering medication may also have positive effects by increasing adherence and compliance to treatment.