Antimicrobial resistance presents one of the most significant threats to public health. This study aimed to examine antibiotic usage within the general population in the Republic of Serbia and their knowledge, attitudes, and behavior concerning this topic. We conducted an online cross-sectional study over two weeks in December 2022, on a sample of 1014 respondents, representative of the Republic of Serbia's population. Predictors of the Antibiotic Knowledge Score (composed of four questions) were analyzed by multivariate ordinal logistic regression. In 2022, 76.8% of the participants from the Serbian population had taken antibiotics, mostly upon a medical prescription, with the most common reasons being upper respiratory tract infections. Only 31.3% of all respondents received any kind of advice about the rational use of antibiotics and half of them changed their opinions on using antibiotics after receiving this information. The average Antibiotic Knowledge Score was 2.6 out of 4, with 32.5% of respondents answering all knowledge questions correctly. The multivariate ordinal logistic regression analysis showed that female gender, higher education level, and the willingness to change opinions regarding the usage of antibiotics after receiving information about the rational use of antibiotics from any available source were significant predictors of better knowledge about antibiotics use. Respondents who were open to changing their opinion after receiving information about the rational use of antibiotics had 28% higher odds of higher antibiotic knowledge scores. This is the first population-level study on public knowledge, attitudes, and practices about antibiotic use in Serbia and therefore the baseline for future research and measuring the impact of potential interventions. Our findings underline the importance of taking into account specific population characteristics, knowledge levels, and attitudes when designing educational and intervention strategies for antibiotic use. Policymakers can leverage these findings to target specific groups and enhance the population's knowledge and practices regarding rational antibiotic usage.

Background/Objectives: Antimicrobial resistance (AMR) is a growing global health threat, requiring an approach that integrates human, animal, and environmental health. Public awareness and responsible antibiotic use are key to combating AMR. This study examines the knowledge, attitudes, and practices related to antibiotic use among the general population in Serbia, exploring their impact on antimicrobial stewardship efforts. Methods: A cross-sectional study was conducted in December 2022 using a three-stage stratified random sample of the Serbian population. The Eurobarometer questionnaire was utilized with permission from the European Commission. Statistical analyses included multivariate ordinal logistic regression to identify predictors of desirable attitudes and behaviors related to antibiotic use. Results: Almost two-thirds of respondents (61.6%) reported not receiving any advice on rational antibiotic use, underscoring the need for education of the general population and raising awareness. A Higher Antibiotic Knowledge Score was associated with a 60% greater likelihood of favorable attitudes and a 21% greater likelihood of rational antibiotic use. Pharmacists were identified as the primary source of antibiotic-related information for the general population in Serbia, but there is limited public interest and awareness of antibiotic use in animals, as well as of the bans on using antibiotics to stimulate growth in farm animals within the EU. Conclusions: Through targeted educational interventions, strengthening interdisciplinary collaboration and AMR control measures is necessary for human and veterinary medicine. Although pharmacists are the primary source of information about antibiotics for the population in Serbia, collaboration with physicians and their empowerment should be introduced to strengthen antimicrobial stewardship further.

Background: Antimicrobial resistance (AMR) is one of the major global public health threats that may lead to severe illness, hospital admissions, treatment failure and increasing of the health care costs. In order to address those challenges, the aims of this study were to examine the antibiotics consumption among the population of health care consumers in the Institute of Public Health of Belgrade, and their knowledge and attitudes regarding antibiotics compared to the inhabitants of EU and Japan. Methods: A cross-sectional study was conducted on a sample of 321 respondents who visited the Institute of Public Health of Belgrade in July 2021. The basic survey instrument was a Eurobarometer questionnaire (with the permission of the Directorate General for Communication European Commission). The obtained data were analyzed by methods of descriptive statistics, which included frequency distribution with percentages. In addition, the Chi-square test was used to examine the difference in frequencies. Results: More than half of all respondents used antibiotics in the previous 12 months (56.6%), majority of them with a doctor's prescription and 57.1% did some pre-testing (blood or urine test, swab) before or at the same time as using antibiotics. They used antibiotics most often for urinary infections (9,3%) and common cold (8,1%). We discovered that the knowledge of our respondents is somewhere in between comparing to the knowledge of the people from EU and Japan. 37.4% of our respondents knew that antibiotics are ineffective against cold and flu; 68.9% knew that unnecessary use of antibiotics makes them ineffective; 55.9% knew that taking antibiotics often leads to side effects such as diarrhea and 50.7% knew that antibiotics don't kill viruses. Conclusions: Knowledge about the antibiotics is insufficient and interventions of education, better informing and awareness of general public are necessary to encourage rational use of antibiotics. Key messages: • It is important to take evidence based interventions to reduce unnecessary use of antibiotics. • This is the first study about antibiotics in Serbia which used the Eurobarometer model of research.

Antimicrobial resistance (AMR) is commonly framed as a consequence of antimicrobial use, yet many conditions that shape resistance arise before treatment is considered and outside clinical care. This review examines contemporary determinants of AMR across human health, animal health, food production, environmental systems, and wider social and institutional contexts. Determinants are organized around common pathways, such as infection burden, antimicrobial exposure, selection pressure, transmission, and systems’ capacity to prevent and control resistance. This pathway-based perspective helps identify critical intervention points in diagnostics, infection prevention, vaccination, water and sanitation, biosecurity, wastewater management, governance, and emergency preparedness. It also shows why surveillance conducted within separate sectors may miss interactions at human, animal, and environmental interfaces. The synthesis supports a broader understanding of AMR literacy that extends beyond knowledge and individual antibiotic behavior to include recognition of interacting determinants, opportunities for prevention, and the different capacities of actors to influence them. Shared responsibility should therefore be understood as differentiated responsibility among individuals, professionals, institutions, industry, and governments. By linking determinants with the pathways through which they act, potential points for intervention, and different levels of responsibility, this review provides a framework for more coherent One Health surveillance, prevention, preparedness, and policy responses.