Leaf mildew is a common disease of tomato leaves. Its detection is an important means to reduce yield loss from the disease and improve tomato quality. In this study, a new method was developed for the multi-source detection of tomato leaf mildew by THz hyperspectral imaging through combining internal and external leaf features. First, multi-source information obtained from tomato leaves of different disease grades was extracted by near-infrared hyperspectral imaging and THz time-domain spectroscopy, while the influence of low-frequency noise was removed by the Savitzky Golay (SG) smoothing algorithm. A genetic algorithm (GA) was used to optimize the selection of the characteristic near-infrared hyperspectral band. Principal component analysis (PCA) was employed to optimize the THz characteristic absorption spectra and power spectrum dimensions. Recognition models were developed for different grades of tomato leaf mildew infestation by incorporating near-infrared hyperspectral imaging, THz absorbance, and power spectra using the backpropagation neural network (BPNN), and the models had recognition rates of 95%, 96.67%, and 95%, respectively. Based on the near-infrared hyperspectral features, THz time-domain spectrum features, and classification model, the probability density of the posterior distribution of tomato leaf health parameter variables was recalculated by a Bayesian network model. Finally, a fusion diagnosis and health evaluation model of tomato leaf mildew with hyperspectral fusion THz was established, and the recognition rate of tomato leaf mildew samples reached 97.12%, which improved the recognition accuracy by 0.45% when compared with the single detection method, thereby achieving the accurate detection of facility diseases.

Airborne crop diseases cause great losses to agricultural production and can affect people’s physical health. Timely monitoring of the situation of airborne disease spores and effective prevention and control measures are particularly important. In this study, a two-stage separation and enrichment microfluidic chip with arcuate pretreatment channel was designed for the separation and enrichment of crop disease spores, which was combined with micro Raman for Raman fingerprinting of disease conidia and quasi identification. The chip was mainly composed of arc preprocessing and two separated enriched structures, and the designed chip was numerically simulated using COMSOL multiphysics5.5, with the best enrichment effect at W2/W1 = 1.6 and W4/W3 = 1.1. The spectra were preprocessed with standard normal variables (SNVs) to improve the signal-to-noise ratio, which was baseline corrected using an iterative polynomial fitting method to further improve spectral features. Raman spectra were dimensionally reduced using principal component analysis (PCA) and stability competitive adaptive weighting (SCARS), support vector machine (SVM) and back-propagation artificial neural network (BPANN) were employed to identify fungal spore species, and the best discrimination effect was achieved using the SCARS-SVM model with 94.31% discrimination accuracy. Thus, the microfluidic-chip- and micro-Raman-based methods for spore capture and identification of crop diseases have the potential to be precise, convenient, and low-cost methods for fungal spore detection.

Plant height is a crucial indicator of crop growth. Rapid measurement of crop height facilitates the implementation and management of planting strategies, ensuring optimal crop production quality and yield. This paper presents a low-cost method for the rapid measurement of multiple lettuce heights, developed using an improved YOLOv8n-seg model and the stacking characteristics of planes in depth images. First, we designed a lightweight instance segmentation model based on YOLOv8n-seg by enhancing the model architecture and reconstructing the channel dimension distribution. This model was trained on a small-sample dataset augmented through random transformations. Secondly, we proposed a method to detect and segment the horizontal plane. This method leverages the stacking characteristics of the plane, as identified in the depth image histogram from an overhead perspective, allowing for the identification of planes parallel to the camera’s imaging plane. Subsequently, we evaluated the distance between each plane and the centers of the lettuce contours to select the cultivation substrate plane as the reference for lettuce bottom height. Finally, the height of multiple lettuce plants was determined by calculating the height difference between the top and bottom of each plant. The experimental results demonstrated that the improved model achieved a 25.56% increase in processing speed, along with a 2.4% enhancement in mean average precision compared to the original YOLOv8n-seg model. The average accuracy of the plant height measurement algorithm reached 94.339% in hydroponics and 91.22% in pot cultivation scenarios, with absolute errors of 7.39 mm and 9.23 mm, similar to the sensor’s depth direction error. With images downsampled by a factor of 1/8, the highest processing speed recorded was 6.99 frames per second (fps), enabling the system to process an average of 174 lettuce targets per second. The experimental results confirmed that the proposed method exhibits promising accuracy, efficiency, and robustness.

Highly sensitive detection of daminozide using terahertz metamaterial sensor

As rice is one of the world’s most important food crops, protecting it from fungal diseases is very important for agricultural production. At present, it is difficult to diagnose rice fungal diseases at an early stage using relevant technologies, and there are a lack of rapid detection methods. This study proposes a microfluidic chip-based method combined with microscopic hyperspectral detection of rice fungal disease spores. First, a microfluidic chip with a dual inlet and three-stage structure was designed to separate and enrich Magnaporthe grisea spores and Ustilaginoidea virens spores in air. Then, the microscopic hyperspectral instrument was used to collect the hyperspectral data of the fungal disease spores in the enrichment area, and the competitive adaptive reweighting algorithm (CARS) was used to screen the characteristic bands of the spectral data collected from the spores of the two fungal diseases. Finally, the support vector machine (SVM) and convolutional neural network (CNN) were used to build the full-band classification model and the CARS filtered characteristic wavelength classification model, respectively. The results showed that the actual enrichment efficiency of the microfluidic chip designed in this study on Magnaporthe grisea spores and Ustilaginoidea virens spores was 82.67% and 80.70%, respectively. In the established model, the CARS-CNN classification model is the best for the classification of Magnaporthe grisea spores and Ustilaginoidea virens spores, and its F1-core index can reach 0.960 and 0.949, respectively. This study can effectively isolate and enrich Magnaporthe grisea spores and Ustilaginoidea virens spores, providing new methods and ideas for early detection of rice fungal disease spores.