This paper presents a conductive polymer based electrospun-nanofibrous-membrane (ENFM) for enzymatic electrochemical glucose sensor. ENFM based sensor electrode shows a wide detection range from 0 mM to 25 mM concentration with a shorter response time of less than 4 sec and the sensitivity of $5.11 \mu\text{A}/ \text{mM}\; \text{cm}^2$ with limit of detection 2.2 μM in a glucose buffer solution. The sensing electrode shows negligible change in current response for upto 50 days. The sensing activity of electrode remained constant indicating a good long-term stability for the glucose sensor due to the direct immobilization of glucose oxidase (GOx) enzyme onto the surface of nanofibers. The electrochemical analysis results demonstrated that the ENFM based glucose sensor provides reliable results in terms of enhanced sensitivity, good stability, durability, and quick response time for glucose detection.

This work presents, silicon carbide nanoparticles (SiCNPs) embedded in a conductive polymer (CP) to be electrospun to fabricate a nanofibrous membrane and a thin-film. Electrochemical enzymatic glucose sensing mechanism of an electrospun nanofibrous membrane (ENFM) of SiCNPs in a CP compared to a spin-coated-thin-film (SCTF) of SiCNPs in a CP. Fiber alignment in the form of a matrix is a key factor that determines the physical properties of nanofiber membrane compared to thin-film. It is found that glucose sensing electrodes formed by a SiCNPs-ENFM has enhanced binding of the glucose oxidase (GO _x ) enzyme within the fibrous membrane as compared to a SiCNPs-SCTF. The SiCNPs-ENFM and SiCNPs-SCTF glucose sensing electrodes were characterized for morphology by using scanning electron microscopy (SEM) and for electrochemical activity by using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods. SiCNPs-ENFM based glucose electrodes shown a detection range from a 0.5 mM to 20 mM concentration with a better sensitivity of $387.57~\mu \text{A}$ /gmMcm ² , and low limit of detection (LOD) 552.89 nM compared to SiCNPs-SCTF with sensitivity of $6.477~\mu \text{A}$ /gmMcm ² and LOD of $60.87~\mu \text{M}$ . The change in current level with SiCNPs-ENFM was ~14% contrast to ~75% with the SiCNPs-SCTF based glucose sensor over 50 days. The electrochemical analysis results demonstrated that the SiCNPs-ENFM electrode provides enhanced sensitivity, better limit of detection (LOD), and durability compared to SiCNPs-SCTF based glucose sensing electrode.