This study presents the development and comprehensive characterization of biopolymer-based nanofibrous composites composed of polyvinyl alcohol (PVA), chitosan (CS), boric acid (BA), and a natural antifungal agent natamycin (NAT), designed for therapeutic applications. A dual-layer 3D-fiber composite (PVA/CS/BA_PVA/NAT) was successfully fabricated using a layer-by-layer 3D bioprinting technique and electro-spinning, integrating BA into the core matrix and NAT into the outer layer. Mechanical tests revealed a significantly improved elastic modulus of 763.04 ± 14.54 MPa and the highest ultimate tensile stress (50.45 ± 2.58 MPa) among all samples. Despite a moderate strain at break (11.77 ± 0.49%), the composite preserved sufficient elasticity suitable for biological interfaces. Morphological assessment via SEM confirmed the successful deposition of continuous and bead-free nanofibers, with controlled fiber alignment and reduced average fiber diameters, especially in the BA-incorporated structure. The dual-layered system displayed enhanced uniformity and structural coherence. The drug release analysis demonstrated sustained NAT delivery over a 90 min period. Kinetic modeling showed a high correlation with the Korsmeyer–Peppas model (R² > 0.99), suggesting diffusion-controlled release, supported by the Korsmeyer–Peppas model’s Fickian diffusion exponent. In contrast, zero- and first-order models exhibited weaker fits, underscoring the relevance of a matrix-based release mechanism governed by the layered configuration. Crucially, antifungal assays against Candida albicans revealed substantial bioactivity. The PVA/CS/BA_PVA/NAT formulation achieved the largest inhibition zone (1.64 ± 0.13 cm), significantly outperforming single-layer controls such as PVA/CS/BA (1.25 ± 0.08 cm) and PVA/CS_PVA/NAT (1.43 ± 0.08 cm), while neat PVA exhibited no inhibition. These results confirm the synergistic antifungal efficacy of BA and NAT within the dual-layer structure. Together, these findings highlight the potential of the 3D-printed PVA/CS/BA_PVA/NAT composite as a mechanically robust, morphologically optimized, and bioactive platform for antifungal therapy and wound-healing applications.

The essential oils (EOs) extracted from sage (Salvia officinalis), rosemary (Rosmarinus officinalis), oregano (Origanum vulgare), eucalyptus sp. and fennel (Foeniculum vulgare) were tested for their antifungal capacities against Penicillium (P.) expansum, Colletotrichum (C.) gloeosporioides and Botrytis (B.) cinerea in vitro and in vivo using golden delicious apples. The main components found in the essential oils of rosemary, sage, fennel, eucalyptus and oregano were camphor (26.25 %), α-thujone (14.95 %) along with 1.8-cineol (13.40 %), trans anethole (80.73 %), cymene (24.90 %) and carvacrol (81.25 %) respectively. The percentage of mycelial growth inhibition was determined using fumigation bioassay and contact bioassay techniques. Oregano EO was found to be the most effective essential oil against fungi species in in vitro and in vivo trials. The descending order of inhibition power of other EOs was noted as eucalyptus, fennel, sage and rosemary. Oregano EO did not stop the fungal growth completely at selected concentrations. However, it was able to limit the growth of fungi (fungistatic) in in vitro bioassays from approximately 80 to 15 mm, from 19 to 4 mm and from 50 to 9 mm in diameter for B. cinerea, P. expansum and C. gloeosporioides respectively.

Very High-resolution (VHR) optical satellites with a ground sampling distance (GSD) of 1m and less for nadir view began with IKONOS in 1999. There are now several VHR optical satellites. A WorldView-2 image compared the advantage of higher-resolution space images for mapping purposes with some lower-resolution VHR images. The orbit altitude of WorldView-2 (WV2) was changed from 767km to 680km in 2011, reducing the GSD in the nadir view from 0.46m to 0.41m. The WV2 image was taken at an incidence angle of 33.3°, resulting in a GSD of 0.49 m times 0.59 m, or 0.54 m on average. The information content analysis confirmed the generally required production scale of 0.05 to 0.1 mm GSD at map scale. This corresponds to a topographic map scale of 1:10,000 for 1 m and 1:5000 for 0.5 m GSD images. This is also based on test fields in İstanbul, Adalar district. The required mapping detail that could be identified using with the VHR space images is dominated by the ground resolution available as the ground sampling distance (GSD). WV2 imagery has proven to help update the GIS and cadastral database.