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(1) Aims: This study was aimed to investigate the effects of organic and inorganic fertilizer application on the soil nutrients and microbiota in tea garden soil. (2) Method: Illumina Hiseq sequencing technique was conducted to analyze the microbial diversity and density in different fertilizer-applied tea garden soil. (3) Results: The results showed that Acidobacteria, Proteobacteria and Actinobacteria were the predominant bacterial species observed in the tea garden soil. Besides, the relative abundance of Basidiomycota, Ascomycota and Zygomycota fungal species were higher in the tea garden soil. Correlation analysis revealed that Acidibacter and Acidothermus were significantly correlated with chemical properties (such as total organic carbon (TOC), total phosphorus (TP) and available phosphorus (AP) contents) of the tea garden soil. Furthermore, all these microbes were abundant in medium rapeseed cake (MRSC) + green manure (GM) treated tea garden soil. (4) Conclusion: Based on the obtained results, we conclude that the application of MRSC + GM could be a preferred fertilizer to increase the soil nutrients (TOC, TP and AP content) and microbial population in the tea garden soil.
Haiping Fu; Huan Li; Peng Yin; Huiling Mei; Jianjie Li; Pinqian Zhou; Yuanjiang Wang; Qingping Ma; Anburaj Jeyaraj; Kuberan Thangaraj; Xuan Chen; Xinghui Li; Guiyi Guo. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil. Sustainability 2021, 13, 2967 .
AMA StyleHaiping Fu, Huan Li, Peng Yin, Huiling Mei, Jianjie Li, Pinqian Zhou, Yuanjiang Wang, Qingping Ma, Anburaj Jeyaraj, Kuberan Thangaraj, Xuan Chen, Xinghui Li, Guiyi Guo. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil. Sustainability. 2021; 13 (5):2967.
Chicago/Turabian StyleHaiping Fu; Huan Li; Peng Yin; Huiling Mei; Jianjie Li; Pinqian Zhou; Yuanjiang Wang; Qingping Ma; Anburaj Jeyaraj; Kuberan Thangaraj; Xuan Chen; Xinghui Li; Guiyi Guo. 2021. "Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil." Sustainability 13, no. 5: 2967.
The plastidial ω-3 fatty acid desaturase catalyses the production of trienoic fatty acids (TAs) in plant chloroplasts and plays an important role in plant responses to environmental stress. In this study, the full-length cDNAs encoding two plastidial ω-3 desaturases, designated CsFAD7 and CsFAD8 (GenBank Accession No. JX943516 and KC847167, respectively), were isolated from the tea plant (Camellia sinensis L.) using RT-PCR and RACE. Codon usage analysis revealed that U- and A-ended codons were preferentially used in these two genes. Sequence analysis showed that the deduced amino acid sequences of CsFAD7 and CsFAD8 had high homology to plastidial ω-3 desaturases from other plant species. Expression analysis by real-time PCR revealed that both genes are tissue-specific and expressed the highest levels in shoots. Meanwhile, CsFAD7 and CsFAD8 responded to various abiotic stresses and hormones, but in very different manners. Taken together, these results suggest that CsFAD7 and CsFAD8 are both responsive to abiotic stress signals; however, they may play very different roles during stress tolerance in tea plants.
Qing-Ping Ma; En You; Jing Wang; Yu Wang; Zhao-Tang Ding. Isolation and expression of CsFAD7 and CsFAD8, two genes encoding ω-3 fatty acid desaturase from Camellia sinensis. Acta Physiologiae Plantarum 2014, 36, 2345 -2352.
AMA StyleQing-Ping Ma, En You, Jing Wang, Yu Wang, Zhao-Tang Ding. Isolation and expression of CsFAD7 and CsFAD8, two genes encoding ω-3 fatty acid desaturase from Camellia sinensis. Acta Physiologiae Plantarum. 2014; 36 (9):2345-2352.
Chicago/Turabian StyleQing-Ping Ma; En You; Jing Wang; Yu Wang; Zhao-Tang Ding. 2014. "Isolation and expression of CsFAD7 and CsFAD8, two genes encoding ω-3 fatty acid desaturase from Camellia sinensis." Acta Physiologiae Plantarum 36, no. 9: 2345-2352.