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(1) Aims: This study was aimed to investigate the effects of organic and inorganic fertilizer application on the soil nutrients and microbiota in tea garden soil. (2) Method: Illumina Hiseq sequencing technique was conducted to analyze the microbial diversity and density in different fertilizer-applied tea garden soil. (3) Results: The results showed that Acidobacteria, Proteobacteria and Actinobacteria were the predominant bacterial species observed in the tea garden soil. Besides, the relative abundance of Basidiomycota, Ascomycota and Zygomycota fungal species were higher in the tea garden soil. Correlation analysis revealed that Acidibacter and Acidothermus were significantly correlated with chemical properties (such as total organic carbon (TOC), total phosphorus (TP) and available phosphorus (AP) contents) of the tea garden soil. Furthermore, all these microbes were abundant in medium rapeseed cake (MRSC) + green manure (GM) treated tea garden soil. (4) Conclusion: Based on the obtained results, we conclude that the application of MRSC + GM could be a preferred fertilizer to increase the soil nutrients (TOC, TP and AP content) and microbial population in the tea garden soil.
Haiping Fu; Huan Li; Peng Yin; Huiling Mei; Jianjie Li; Pinqian Zhou; Yuanjiang Wang; Qingping Ma; Anburaj Jeyaraj; Kuberan Thangaraj; Xuan Chen; Xinghui Li; Guiyi Guo. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil. Sustainability 2021, 13, 2967 .
AMA StyleHaiping Fu, Huan Li, Peng Yin, Huiling Mei, Jianjie Li, Pinqian Zhou, Yuanjiang Wang, Qingping Ma, Anburaj Jeyaraj, Kuberan Thangaraj, Xuan Chen, Xinghui Li, Guiyi Guo. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil. Sustainability. 2021; 13 (5):2967.
Chicago/Turabian StyleHaiping Fu; Huan Li; Peng Yin; Huiling Mei; Jianjie Li; Pinqian Zhou; Yuanjiang Wang; Qingping Ma; Anburaj Jeyaraj; Kuberan Thangaraj; Xuan Chen; Xinghui Li; Guiyi Guo. 2021. "Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil." Sustainability 13, no. 5: 2967.
The quality and flavor of green tea can be affected by various factors, which are closely related to the metabolite composition of tea. In this study, 66 Xinyang Maojian tea (XYMJ) samples produced by four cultivars, grown in different elevations and manufactured by different processing methods were analyzed by untargeted ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry and chemometrics. 1912 ion features were detected and 95 metabolites were identified tentatively through a customized in-house library. Projection to latent structures discriminate analysis showed high capability to explain the cultivar variation. 54 metabolites were found to be responsible for the differentiation of the four cultivars. 27 metabolites including epigallocatechin gallate, epicatechin gallate, theanine, theogallin showed close correlation with elevation, resulting enhanced umami flavor of the high elevation tea. The differences between manual and mechanical tea were not significant. This comprehensive study is of great reference value for other types of tea.
Huijun Wang; Xueli Cao; Zhengfang Yuan; Guiyi Guo. Untargeted metabolomics coupled with chemometrics approach for Xinyang Maojian green tea with cultivar, elevation and processing variations. Food Chemistry 2021, 352, 129359 .
AMA StyleHuijun Wang, Xueli Cao, Zhengfang Yuan, Guiyi Guo. Untargeted metabolomics coupled with chemometrics approach for Xinyang Maojian green tea with cultivar, elevation and processing variations. Food Chemistry. 2021; 352 ():129359.
Chicago/Turabian StyleHuijun Wang; Xueli Cao; Zhengfang Yuan; Guiyi Guo. 2021. "Untargeted metabolomics coupled with chemometrics approach for Xinyang Maojian green tea with cultivar, elevation and processing variations." Food Chemistry 352, no. : 129359.
Background Heat stress factors (Hsfs) play vital roles in signal transduction pathways operating in responses to environmental stresses. However, Hsf gene family has not been thoroughly explored in tea plant (Camellia sinensis L.). Results In this study, we identified 25 CsHsf genes in C. sinensis that were separated by phylogenetic analysis into three sub-families (i.e., A, B, and C). Gene structures, conserved domains and motifs analyses indicated that the CsHsf members in each class were relatively conserved. Various cis-acting elements involved in plant growth regulation, hormone responses, stress responses, and light responses were located in the promoter regions of CsHsfs. Furthermore, degradome sequencing analysis revealed that 7 CsHsfs could be targeted by 9 miRNAs. The expression pattern of each CsHsf gene was significantly different in eight tissues. Many CsHsfs were differentially regulated by drought, salt, and heat stresses, as well as exogenous abscisic acid (ABA) and Ca2+. In addition, CsHsfA2 was located in the nucleus. Heterologous expression of CsHsfA2 improved thermotolerance in transgenic yeast, suggesting its potential role in the regulation of heat stress response. Conclusions A comprehensive genome-wide analysis of Hsf in C. sinensis present the global identification and functional prediction of CsHsfs. Most of them were implicated in a complex gene regulatory network controlling various abiotic stress responses and signal transduction pathways in tea plants. Additionally, heterologous expression of CsHsfA2 increased thermotolerance of transgenic yeast. These findings provide new insights into the functional divergence of CsHsfs and a basis for further research on CsHsfs functions.
Xuyang Zhang; Wenluan Xu; Dejiang Ni; Mingle Wang; Guiyi Guo. Genome-wide characterization of tea plant (Camellia sinensis) Hsf transcription factor family and role of CsHsfA2 in heat tolerance. BMC Plant Biology 2020, 20, 1 -17.
AMA StyleXuyang Zhang, Wenluan Xu, Dejiang Ni, Mingle Wang, Guiyi Guo. Genome-wide characterization of tea plant (Camellia sinensis) Hsf transcription factor family and role of CsHsfA2 in heat tolerance. BMC Plant Biology. 2020; 20 (1):1-17.
Chicago/Turabian StyleXuyang Zhang; Wenluan Xu; Dejiang Ni; Mingle Wang; Guiyi Guo. 2020. "Genome-wide characterization of tea plant (Camellia sinensis) Hsf transcription factor family and role of CsHsfA2 in heat tolerance." BMC Plant Biology 20, no. 1: 1-17.
Temperature is one of the most important environmental factors limiting tea plant growth and tea production. Previously we reported that both Ca2+ and ROS signals play important roles in tea plant cold acclimation. Here, we identified 26 CsCPK transcripts, analyzed their phylogenetic and sequence characters, and detected their transcriptions to monitor Ca2+ signaling status. Tissue-specific expression profiles indicated that most CsCPK genes were constitutively expressed in tested tissues, suggesting their possible roles in development. Cold along with calcium inhibitor assays suggested that CsCPKs are important cold regulators and CsCPK30/5/4/9 maybe the key members. Moreover, LaCl3 or EGTA pre-treatment could result in impaired Ca2+ signaling and compromised cold-responding network, but higher catechins accumulation revealed their potential positive roles in cold responses. Those findings indicated that catechins and other secondary metabolites in tea plant may form an alternative cold-responding network that closely correlated with Ca2+ signaling status.
Changqing Ding; Lei Lei; Lina Yao; Lu Wang; Xinyuan Hao; Nana Li; Yuchun Wang; Peng Yin; Guiyi Guo; Yajun Yang; Xinchao Wang. The involvements of calcium-dependent protein kinases and catechins in tea plant [Camellia sinensis (L.) O. Kuntze] cold responses. Plant Physiology and Biochemistry 2019, 143, 190 -202.
AMA StyleChangqing Ding, Lei Lei, Lina Yao, Lu Wang, Xinyuan Hao, Nana Li, Yuchun Wang, Peng Yin, Guiyi Guo, Yajun Yang, Xinchao Wang. The involvements of calcium-dependent protein kinases and catechins in tea plant [Camellia sinensis (L.) O. Kuntze] cold responses. Plant Physiology and Biochemistry. 2019; 143 ():190-202.
Chicago/Turabian StyleChangqing Ding; Lei Lei; Lina Yao; Lu Wang; Xinyuan Hao; Nana Li; Yuchun Wang; Peng Yin; Guiyi Guo; Yajun Yang; Xinchao Wang. 2019. "The involvements of calcium-dependent protein kinases and catechins in tea plant [Camellia sinensis (L.) O. Kuntze] cold responses." Plant Physiology and Biochemistry 143, no. : 190-202.