Micro-organisms are abundant in nature and can also be found in hospital settings, causing high rates of infections. This study aimed to identify bacteria isolated from a veterinary hospital, as well as to perform antimicrobial susceptibility testing using the disk diffusion method (Kirby–Bauer), biofilm production tests using 96-well polystyrene microtiter plates and crystal violet dye, and genetic analysis of the ica operon of Staphylococcus isolates. Three collections were made from eleven surfaces and objects in the hospital’s non-critical areas (general areas) and critical areas (surgical center), totaling thirty-three samples. A total of 66 different bacterial isolates were obtained, with 77% (51/66) Gram-positive and 23% (29/66) Gram-negative. Resistance profiles were found for multidrug-resistance (MDR), methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant Staphylococcus epidermidis (MRSE), and other unidentified species of methicillin-resistant coagulase-negative (MRCNS) and extended-spectrum beta-lactamase (ESBL), as well as biofilm production rates of 57% (38/66) of the isolates. Analysis of the operon genes for Staphylococcus sp. showed divergence in some samples when compared to the phenotypic test performed. In summary, there is a high presence of micro-organisms with resistance and virulence factors spread throughout the various areas of the veterinary hospital.

Microorganisms are abundantly dispersed in nature and can also be found in hospital environments, causing high rates of infections. This study aims to identify bacteria isolated from a veterinary hospital as well as carry out antimicrobial susceptibility testing, biofilm production tests, and genetic analysis of the ica operon of the isolates. Three collections were carried out on 11 surfaces and objects from general areas and the hospital’s operating room, totaling 33 samples, with 66 different bacterial species isolated, 77% (51/66) of them gram-positive and 23% (29/66) gram-negative. MDR, MRSA, MRS and ESBL resistance profiles were found, as well as biofilm production rates of 57% (38/66) of the isolates. Analysis of operon genes for Staphylococcus sp. demonstrated divergence in some samples when compared to the phenotypic test performed. In summary, there is a high presence of microorganisms with resistance and virulence factors spread throughout the various facilities of the veterinary hospital.