Micro-organisms are abundant in nature and can also be found in hospital settings, causing high rates of infections. This study aimed to identify bacteria isolated from a veterinary hospital, as well as to perform antimicrobial susceptibility testing using the disk diffusion method (Kirby–Bauer), biofilm production tests using 96-well polystyrene microtiter plates and crystal violet dye, and genetic analysis of the ica operon of Staphylococcus isolates. Three collections were made from eleven surfaces and objects in the hospital’s non-critical areas (general areas) and critical areas (surgical center), totaling thirty-three samples. A total of 66 different bacterial isolates were obtained, with 77% (51/66) Gram-positive and 23% (29/66) Gram-negative. Resistance profiles were found for multidrug-resistance (MDR), methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant Staphylococcus epidermidis (MRSE), and other unidentified species of methicillin-resistant coagulase-negative (MRCNS) and extended-spectrum beta-lactamase (ESBL), as well as biofilm production rates of 57% (38/66) of the isolates. Analysis of the operon genes for Staphylococcus sp. showed divergence in some samples when compared to the phenotypic test performed. In summary, there is a high presence of micro-organisms with resistance and virulence factors spread throughout the various areas of the veterinary hospital.

Microorganisms are abundantly dispersed in nature and can also be found in hospital environments, causing high rates of infections. This study aims to identify bacteria isolated from a veterinary hospital as well as carry out antimicrobial susceptibility testing, biofilm production tests, and genetic analysis of the ica operon of the isolates. Three collections were carried out on 11 surfaces and objects from general areas and the hospital’s operating room, totaling 33 samples, with 66 different bacterial species isolated, 77% (51/66) of them gram-positive and 23% (29/66) gram-negative. MDR, MRSA, MRS and ESBL resistance profiles were found, as well as biofilm production rates of 57% (38/66) of the isolates. Analysis of operon genes for Staphylococcus sp. demonstrated divergence in some samples when compared to the phenotypic test performed. In summary, there is a high presence of microorganisms with resistance and virulence factors spread throughout the various facilities of the veterinary hospital.

To conduct a serological screening for toxoplasmosis in the heel prick test and to evaluate its epidemiological aspects in newborns and postpartum women in Jataí, Goiás.

Realizar a triagem sorológica da toxoplasmose no teste do pezinho e avaliar os aspectos epidemiológicos em recém-nascidos e puérperas de Jataí, Goiás.

This study analyzed the biofilm production of S. pseudintermedius isolated from the ocular surface of dogs with ophthalmopathies and investigated the genes involved. Of the 63 isolates, 50.8% produced biofilm, 71.9% being weak, 25% moderate and 3.1% strong. All methicillin-resistant isolates had weak to moderate production, and multiresistant isolates produced biofilm at different intensities. Entropion was the ocular condition with the most biofilm-producing isolates, followed by keratoconjunctivitis sicca. A strong strain was identified in a dog with chalazion. The nuc gene was detected in 42.8% of isolates, while icaA and icaD were found together in 68.2%, icaD alone in 3.2%, and neither gene in 26.9%. Among genetically identified isolates, 74.1% had both genes, while 25.9% did not. Agreement between phenotypic and genotypic tests was poor (Kappa = 0.1046), highlighting the need for combined methods to identify biofilm production. S. pseudintermedius showed significant biofilm formation capacity, especially in species such as S. pseudintermedius, which represents a relevant single health problem.

Cutaneous melanoma is a malignant neoplasm derived from melanocytes and stands out for its high lethality compared to other types of skin cancer. In this context, superficial spreading melanoma (SSM) is the subtype responsible for most diagnoses. The BRAF V600E mutation showed high relevance in the evolution and prognosis of SSM due to its role in activating the MAPK/ERK signaling pathway, contributing to deregulated cell proliferation and tumor progression. This study aimed to review the scientific literature from the last five years to deepen the understanding of the molecular profile involved in melanoma progression, with emphasis on the BRAF V600E mutation and its prognostic and therapeutic implications. The methodology consisted of a bibliographic review using the PubMed, Virtual Health Library (BVS), and SciELO databases. The results demonstrated the role of the BRAF mutation as a biomarker and its association with greater tumor aggressiveness, metastatic potential, and impact on the response to targeted therapies, while also highlighting the challenges and limitations of this biomarker, reinforcing the importance of identifying the molecular profile for the personalization of melanoma treatment. O melanoma cutâneo é uma neoplasia maligna derivada dos melanócitos, destacando-se por sua alta letalidade em comparação aos demais cânceres de pele. Nesse contexto, o melanoma extensivo superficial (SSM) caracteriza-se como o subtipo responsável pela maioria dos diagnósticos. A mutação BRAF V600E apresentou alta relevância na evolução e no prognóstico do SSM, devido à sua participação na ativação da via MAPK/ERK, contribuindo para a proliferação celular desregulada e o avanço tumoral. Este trabalho teve como objetivo revisar a literatura científica dos últimos cinco anos, a fim de aprofundar a compreensão sobre o perfil molecular envolvido na evolução do melanoma, com ênfase na relação da mutação BRAF V600E e suas implicações prognósticas e terapêuticas. A metodologia consistiu em uma revisão bibliográfica nas bases de dados PubMed, Biblioteca Virtual em Saúde (BVS) e SciELO. Os resultados evidenciaram o papel da mutação BRAF como biomarcador e sua associação à maior agressividade tumoral, potencial metastático e impacto nas respostas às terapias-alvo, além de destacarem os desafios e limitações desse marcador, reforçando a importância da identificação do perfil molecular para a personalização do tratamento do melanoma.