Quinolone- and fluoroquinolone-resistant Escherichia coli strains harbor fewer virulence factors than susceptible strains. The reasons underlying this correlation are incompletely understood. We investigated the phylogenetic background, the presence of the papC, hlyA , and cnf1 (pathogenicity island II _J96 -associated), fimA, iss , and iutA genes, and the presence of type 1 fimbriae, P fimbriae, and hemolysin in 243 urinary E. coli isolates resistant only to quinolones (8%), resistant to both quinolones and fluoroquinolones (51%), or susceptible to both drugs (41%). Group B2 accounted for 56% of the isolates, showing a significantly higher prevalence among fluoroquinolone-susceptible strains than among resistant strains (65% versus 50% [ P = 0.03]). hly and cnf1 were significantly more associated with susceptibility ( P < 0.001) and with group B2 ( P < 0.001 for group B2 versus groups A and D). However, within group B2, fluoroquinolone-resistant strains showed lower prevalences of papC, hlyA , and cnf1 than their susceptible counterparts ( P < 0.001). In contrast, the incidence of iutA appeared higher for refractory isolates, including group B2, than for susceptible isolates ( P < 0.001). Only in group B2 did fluoroquinolone-resistant strains reveal a lesser ability to agglutinate Saccharomyces cerevisiae (7%) than quinolone-resistant (87%) and susceptible (80%) isolates, despite uniform possession of fimA genes. No similar contrast emerged for expression of hemolysin and P fimbriae. Mutations conferring quinolone and fluoroquinolone resistance may thus require a particular genetic background, not strictly correlated with phylogenetic groups. More interestingly, the mutational event itself can affect the expression of type 1 fimbriae, at least in the prevalent and complex B2 strains.

Discarding is a common practice in fisheries. Total discards are estimated to be about 30 million tons, representing around 23% of worldwide catches. Discarding is an undesirable practice, not only because of the waste of resources, but also because of its contribution to the overexploitation of fish stocks. Several countries have already established discard bans, to different extents (e.g., Norway, Iceland, Chile, New Zealand). The EU’s landing obligation (discard ban) is a major measure of the latest reform of the Common Fisheries Policy for EU fisheries. It aims to reduce unwanted catches in EU fisheries, by incentivizing improved selectivity and restoring fish stocks to levels that can sustain the maximum production over time without harming the biodiversity and the capacity of future generations to obtain fish. However, banning discards will inevitably induce diverse short- and long-term ecological, economic, and social impacts, which may determine whether the landing obligation’s objectives will be achieved.

Summary: 1. The development of ecosystem approaches to environmental management implies the need to account for multiple pressures on ecosystems. Trends in multiple metrics that respond differently to changes in major environmental pressures need to be combined to evaluate the impacts of fishing and environmental changes on fish communities. 2. An exploited fish community is viewed as a three‐level food chain in which the two upper levels, or functional groups, are targeted by fishing fleets, while the lowest level is subject to environmental variation. Qualitative modelling is used to predict changes at the two upper levels, that is, top‐down vs. bottom‐up perturbations. Abundance and length metrics are calculated from survey data for 14 Mediterranean and East‐Atlantic groundfish shelf communities at both population and functional group levels. The joint likelihood of time trends in metrics is used to evaluate the evidence for different causes of changes. 3. A wide diversity of impacts is found to have equal evidence at the population level within each community. Consistency between the impacts identified and changes in pressures known from independent information is found at the functional group and community level. The results suggest that there is some compensation between species within functional groups. 4. Synthesis and applications. The method can be used to conduct an integrated assessment of community dynamics subject to multiple pressures. Joint trends in metrics provide evidence of which known pressures are having an impact on the community, and thus, which management actions should be taken to mitigate these changes.