In the present work, the influence of filling rate on macrosegregation in a 40-Metric Ton (MT) ingot of a high-strength low-carbon steel was studied using finite element (FE) simulation. The modelling of the filling and solidification processes were realized with a two-phase (liquid-solid) multiscale 3D model. The liquid flow induced by the pouring jet, the thermosolutal convection, and the thermomechanical deformation of the solid phase were taken into consideration. Two filling rates were examined, representing the upper and lower manufacturing limits for casting of large size ingots made of high strength steels for applications in energy and transportation industries. The evolution of solute transport, as well as its associated phenomena throughout the filling and cooling stages, were also investigated. It was found that increasing the filling rate reduced macrosegregation intensity in the upper section, along the centerline and in the mid-radius regions of the ingot. The results were analyzed in the framework of heat and mass transfer theories, liquid flow dynamics, and macrosegregation formation mechanisms.