Maximum depth of penetration (DOP) is among the most relevant parameters in quality assurance programs for Ultrasound (US) scanners. Nowadays, a generally-accepted protocol for DOP estimation is still awaited and, in common practice, DOP is visually assessed despite the low accuracy. To overcome the eye-based assessment subjectivity, automatic image analysis methods have been proposed in literature. The present work focuses on a novel automatic method, namely the adaptive Signal to Noise Ratio (SNR) threshold method (AdSTM), developed in the MATLAB environment, by comparing it with an existing automatic approach, namely the tangent threshold method (TTM), and the mean judgment of eight observers (naked eye method). The three investigated methods were applied on data acquired from four US scanners for general purpose imaging, equipped with linear, convex, and vector array probes. Tests were carried out in two different configuration settings (raw scanner and default preset working conditions). AdSTM outcomes were tested by means of Monte Carlo Simulations. Most of measurement results were compatible despite the fact that the AdSTM seemed to be more sensitive and faster than the TTM. The results analysis confirms the higher dispersion of the naked eye method in DOP assessment with respect to the proposed automatic methods.

In the absence of standard procedures for testing 3D-printed soft polymers, an experimental protocol was proposed to assess the suitability of Flexible 80A Resin for a pediatric trachea anatomical 3D model for surgical simulation. Eighteen specimens printed via stereolithography are involved, including anatomical, cylindrical, and dog-bone shapes, to investigate the geometry effect on measured properties. Static tensile tests revealed that using standardized dog-bone specimens as a reference for the material’s Young’s modulus leads to a mean absolute percentage error (MAPE) up to 50% compared to anatomical specimens. Measurement uncertainty combined repeatability with input errors, and the ANOVA test confirmed the need for dedicated mechanical measurements when evaluating complex 3D-printed geometries. The study concludes the suitability of selected material: the average elastic modulus of anatomical specimens was 4.75 MPa, closely matching values reported for tracheal tissue in the literature, with a MAPE of only 2%. Dynamic mechanical tests showed trachea-like viscoelasticity: anatomical specimens were consistently stiffer and more dissipative than cylindrical ones. Creep tests confirmed the viscoelastic behavior simulating airway time scales. The anatomical specimens exhibit faster local relaxation, while cylindrical ones show slower long-term relaxation, both modeled by a two-element generalized Maxwell model (R² = 0.99 and 0.98).