This study presents an innovative unmanned aerial vehicle (UAV)-based intelligent detection method utilizing an improved Faster Region-based Convolutional Neural Network (Faster R-CNN) architecture to address the inefficiency and inaccuracy inherent in manual wheat spike counting. We systematically collected a high-resolution image dataset (2000 images, 4096 × 3072 pixels) covering key growth stages (heading, grain filling, and maturity) of winter wheat (Triticum aestivum L.) during 2022–2023 using a DJI M300 RTK equipped with multispectral sensors. The dataset encompasses diverse field scenarios under five fertilization treatments (organic-only, organic–inorganic 7:3 and 3:7 ratios, inorganic-only, and no fertilizer) and two irrigation regimes (full and deficit irrigation), ensuring representativeness and generalizability. For model development, we replaced conventional VGG16 with ResNet-50 as the backbone network, incorporating residual connections and channel attention mechanisms to achieve 92.1% mean average precision (mAP) while reducing parameters from 135 M to 77 M (43% decrease). The GFLOPS of the improved model has been reduced from 1.9 to 1.7, an decrease of 10.53%, and the computational efficiency of the model has been improved. Performance tests demonstrated a 15% reduction in missed detection rate compared to YOLOv8 in dense canopies, with spike count regression analysis yielding R² = 0.88 (p < 0.05) against manual measurements and yield prediction errors below 10% for optimal treatments. To validate robustness, we established a dedicated 500-image test set (25% of total data) spanning density gradients (30–80 spikes/m²) and varying illumination conditions, maintaining >85% accuracy even under cloudy weather. Furthermore, by integrating spike recognition with agronomic parameters (e.g., grain weight), we developed a comprehensive yield estimation model achieving 93.5% accuracy under optimal water–fertilizer management (70% ETc irrigation with 3:7 organic–inorganic ratio). This work systematically addresses key technical challenges in automated spike detection through standardized data acquisition, lightweight model design, and field validation, offering significant practical value for smart agriculture development.

This study presents a high-performance silicon nanowire (SiNW) array biosensor for the combined detection of two key colorectal cancer (CRC) biomarkers: circulating tumor DNA (ctDNA) and carcinoembryonic antigen (CEA). The device was fabricated using conventional micromachining techniques, enabling the integration of dual SiNW arrays on a single chip with precise control over structure and surface functionalization. Specific probe DNA and anti-CEA antibodies were immobilized on distinct array regions to facilitate targeted binding. The biosensor demonstrated exceptional performance, achieving an ultralow detection limit of 10 aM for ctDNA with a linear range from 0.1 fM to 10 pM, and a sensitivity of 1 fg/mL for CEA. It exhibited high selectivity against interfering substances, including single-base mismatched DNA and non-specific proteins, and maintained robust performance in human serum samples. The platform offers a scalable, label-free, and real-time detection solution with significant potential for application in early CRC screening and personalized medicine.

Winter wheat yield prediction is critical for optimizing field management plans and guiding agricultural production. To address the limitations of conventional manual yield estimation methods, including low efficiency and poor interpretability, this study innovatively proposes an intelligent yield estimation method based on a convolutional neural network (CNN). A comprehensive two-factor (fertilization × irrigation) controlled field experiment was designed to thoroughly validate the applicability and effectiveness of this method. The experimental design comprised two irrigation treatments, sufficient irrigation (C) at 750 m³ ha⁻¹ and deficit irrigation (M) at 450 m³ ha⁻¹, along with five fertilization treatments (at a rate of 180 kg N ha⁻¹): (1) organic fertilizer alone, (2) organic–inorganic fertilizer blend at a 7:3 ratio, (3) organic–inorganic fertilizer blend at a 3:7 ratio, (4) inorganic fertilizer alone, and (5) no fertilizer control. The experimental protocol employed a DJI M300 RTK unmanned aerial vehicle (UAV) equipped with a multispectral sensor to systematically acquire high-resolution growth imagery of winter wheat across critical phenological stages, from heading to maturity. The acquired multispectral imagery was meticulously annotated using the Labelme professional annotation tool to construct a comprehensive experimental dataset comprising over 2000 labeled images. These annotated data were subsequently employed to train an enhanced CNN model based on ResNet50 architecture, which achieved automated generation of panicle density maps and precise panicle counting, thereby realizing yield prediction. Field experimental results demonstrated significant yield variations among fertilization treatments under sufficient irrigation, with the 3:7 organic–inorganic blend achieving the highest actual yield (9363.38 ± 468.17 kg ha⁻¹) significantly outperforming other treatments (p < 0.05), confirming the synergistic effects of optimized nitrogen and water management. The enhanced CNN model exhibited superior performance, with an average accuracy of 89.0–92.1%, representing a 3.0% improvement over YOLOv8. Notably, model accuracy showed significant correlation with yield levels (p < 0.05), suggesting more distinct panicle morphological features in high-yield plots that facilitated model identification. The CNN’s yield predictions demonstrated strong agreement with the measured values, maintaining mean relative errors below 10%. Particularly outstanding performance was observed for the organic fertilizer with full irrigation (5.5% error) and the 7:3 organic-inorganic blend with sufficient irrigation (8.0% error), indicating that the CNN network is more suitable for these management regimes. These findings provide a robust technical foundation for precision farming applications in winter wheat production. Future research will focus on integrating this technology into smart agricultural management systems to enable real-time, data-driven decision making at the farm scale.