The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38‐Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22,260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bacillus thuringiensis (Bt) resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bt toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

ATP-binding cassette (ABC) transporters, a large class of transmembrane proteins, are widely found in organisms and play an important role in the transport of xenobiotics. Insect ABC transporters are involved in insecticide detoxification and Bacillus thuringiensis (Bt) toxin perforation. The complete ABC transporter is composed of two hydrophobic transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). Conformational changes that are needed for their action are mediated by ATP hydrolysis. According to the similarity among their sequences and organization of conserved ATP-binding cassette domains, insect ABC transporters have been divided into eight subfamilies (ABCA–ABCH). This review describes the functions and mechanisms of ABC transporters in insecticide detoxification, plant toxic secondary metabolites transport and insecticidal activity of Bt toxin. With improved understanding of the role and mechanisms of ABC transporter in resistance to insecticides and Bt toxins, we can identify valuable target sites for developing new strategies to control pests and manage resistance and achieve green pest control.

Extensive use of chemical pesticides poses a great threat to the environment and food safety. The discovery of Bacillus thuringiensis (Bt) toxins with effective insecticidal activity against pests and the development of transgenic technology of plants opened a new era of pest control. Transgenic Bt crops, including maize, cotton and soya bean, have now been produced and commercialized to protect against about 30 major coleopteran and lepidopteran pests, greatly benefiting the environment and the economy. However, with the long-term cultivation of Bt crops, some target pests have gradually developed resistance. Numerous studies have indicated that mutations in genes for toxins activation, toxin-binding and insect immunization are important sources in Bt resistance. An in-depth exploration of the corresponding Bt-resistance mechanisms will aid in the design of new strategies to prevent and control pests. Future research will focus on Bt crops expressing new genes and multiple genes to control a broader range of pests as part of an integrated pest management programme. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.