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Swapan Chakrabarty
Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen China

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Research article
Published: 04 November 2020 in Pest Management Science
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BACKGROUND The fall armyworm Spodoptera frugiperda is a major agricultural pest that has invaded the East Hemisphere since 2016, generating a serious threat to the food security worldwide including Africa and Asia. The Cry toxins produced by Bacillus thuringiensis (Bt) have been shown to be effective against this insect pest. In different insect ABC transporters (ABCC2 or ABCC3) have been shown to be involved as receptors of some Cry1 toxins. Here we analyzed the role of SfABCC2 and SfABCC3 in the toxicity of Cry1Fa and Cry1Ab toxins in this insect pest. RESULTS Two S. frugiperda SfABCC2 and SfABCC3 knockout strains, coding for potential functional Bt receptors, were created using CRISPR/Cas9 genome editing system. Both knockout strains showed resistance to both Cry1Fa and Cry1Ab toxins compared with the susceptible strain. SfABCC2 knockout strain showed higher resistance to both Cry toxins than SfABCC3 knockout strain, suggesting a major role of SfABCC2 in the mode of action of these Cry toxins. In addition, expression of SfABCC2 and SfABCC3 genes in Trichoplusia ni Hi5 cells also increased the susceptibility to Cry1Ab and Cry1Fa toxins, in agreement with the genome editing results. The double knockout of SfABCC2 and SfABCC3 strain was not viable in contrast to other lepidopteran species. Furthermore, we report here that SfABCC2 or SfABCC3 knockout strains increased their susceptibility to abamectin and spinosad insecticides. CONCLUSION We provide functional evidence that in S. frugiperda these two ABCC transporters serve as receptors of Bt Cry1Fa and Cry1Ab toxins. This article is protected by copyright. All rights reserved.

ACS Style

Minghui Jin; Yanchao Yang; YinXue Shan; Swapan Chakrabarty; Ying Cheng; Mario Soberón; Alejandra Bravo; Kaiyu Liu; Kongming Wu; Yutao Xiao. Two ABC transporters are differentially involved in the toxicity of two Bacillus thuringiensis Cry1 toxins to the invasive crop‐pest Spodoptera frugiperda (J. E. Smith). Pest Management Science 2020, 77, 1492 -1501.

AMA Style

Minghui Jin, Yanchao Yang, YinXue Shan, Swapan Chakrabarty, Ying Cheng, Mario Soberón, Alejandra Bravo, Kaiyu Liu, Kongming Wu, Yutao Xiao. Two ABC transporters are differentially involved in the toxicity of two Bacillus thuringiensis Cry1 toxins to the invasive crop‐pest Spodoptera frugiperda (J. E. Smith). Pest Management Science. 2020; 77 (3):1492-1501.

Chicago/Turabian Style

Minghui Jin; Yanchao Yang; YinXue Shan; Swapan Chakrabarty; Ying Cheng; Mario Soberón; Alejandra Bravo; Kaiyu Liu; Kongming Wu; Yutao Xiao. 2020. "Two ABC transporters are differentially involved in the toxicity of two Bacillus thuringiensis Cry1 toxins to the invasive crop‐pest Spodoptera frugiperda (J. E. Smith)." Pest Management Science 77, no. 3: 1492-1501.

Resource article
Published: 03 July 2020 in Molecular Ecology Resources
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The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda ) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38‐Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22,260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bacillus thuringiensis (Bt) resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bt toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

ACS Style

Lei Zhang; Bo Liu; Weigang Zheng; Conghui Liu; Dandan Zhang; Shengyuan Zhao; Zaiyuan Li; Pengjun Xu; Kenneth Wilson; Amy Withers; Christopher M. Jones; Judith A. Smith; Gilson Chipabika; Donald L. Kachigamba; Kiwoong Nam; Emmanuelle D’Alençon; Bei Liu; Xinyue Liang; Minghui Jin; Chao Wu; Swapan Chakrabarty; Xianming Yang; Yuying Jiang; Jie Liu; Xiaolin Liu; Weipeng Quan; Guirong Wang; Wei Fan; Wanqiang Qian; Kongming Wu; Yutao Xiao. Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. Molecular Ecology Resources 2020, 20, 1682 -1696.

AMA Style

Lei Zhang, Bo Liu, Weigang Zheng, Conghui Liu, Dandan Zhang, Shengyuan Zhao, Zaiyuan Li, Pengjun Xu, Kenneth Wilson, Amy Withers, Christopher M. Jones, Judith A. Smith, Gilson Chipabika, Donald L. Kachigamba, Kiwoong Nam, Emmanuelle D’Alençon, Bei Liu, Xinyue Liang, Minghui Jin, Chao Wu, Swapan Chakrabarty, Xianming Yang, Yuying Jiang, Jie Liu, Xiaolin Liu, Weipeng Quan, Guirong Wang, Wei Fan, Wanqiang Qian, Kongming Wu, Yutao Xiao. Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. Molecular Ecology Resources. 2020; 20 (6):1682-1696.

Chicago/Turabian Style

Lei Zhang; Bo Liu; Weigang Zheng; Conghui Liu; Dandan Zhang; Shengyuan Zhao; Zaiyuan Li; Pengjun Xu; Kenneth Wilson; Amy Withers; Christopher M. Jones; Judith A. Smith; Gilson Chipabika; Donald L. Kachigamba; Kiwoong Nam; Emmanuelle D’Alençon; Bei Liu; Xinyue Liang; Minghui Jin; Chao Wu; Swapan Chakrabarty; Xianming Yang; Yuying Jiang; Jie Liu; Xiaolin Liu; Weipeng Quan; Guirong Wang; Wei Fan; Wanqiang Qian; Kongming Wu; Yutao Xiao. 2020. "Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China." Molecular Ecology Resources 20, no. 6: 1682-1696.

Journal article
Published: 29 April 2020 in Insect Biochemistry and Molecular Biology
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Cotton bollworm (Helicoverpa armigera) is the major insect herbivore of cotton plants. As its larvae feed and grow on cotton, H. armigera can likely tolerate gossypol, the main defense metabolite produced by cotton plants, through detoxification and sequestration mechanisms. Recent reports have shown that various P450 monooxygenases and UDP-glycosyltransferases in H. armigera are involved in gossypol detoxification, while the roles of ABC transporters, another gene family widely associated with metabolite detoxification, remain to be elucidated. Here, we show that ingestion of gossypol-infused artificial diet and cotton leaves significantly induced the expression of HaABCB6 in H. armigera larvae. Knockdown and knockout of HaABCB6 increased sensitivity of H. armigera larvae to gossypol. Moreover, HaABCB6-GFP fusion protein was localized on lysosomes in Hi5 cells and its overexpression significantly enhanced gossypol tolerance in vitro. These experimental results strongly support that HaABCB6 plays an important role in gossypol detoxification by H. armigera.

ACS Style

Minghui Jin; Ying Cheng; Xueqin Guo; Meizhi Li; Swapan Chakrabarty; Kaiyu Liu; Kongming Wu; Yutao Xiao. Down-regulation of lysosomal protein ABCB6 increases gossypol susceptibility in Helicoverpa armigera. Insect Biochemistry and Molecular Biology 2020, 122, 103387 .

AMA Style

Minghui Jin, Ying Cheng, Xueqin Guo, Meizhi Li, Swapan Chakrabarty, Kaiyu Liu, Kongming Wu, Yutao Xiao. Down-regulation of lysosomal protein ABCB6 increases gossypol susceptibility in Helicoverpa armigera. Insect Biochemistry and Molecular Biology. 2020; 122 ():103387.

Chicago/Turabian Style

Minghui Jin; Ying Cheng; Xueqin Guo; Meizhi Li; Swapan Chakrabarty; Kaiyu Liu; Kongming Wu; Yutao Xiao. 2020. "Down-regulation of lysosomal protein ABCB6 increases gossypol susceptibility in Helicoverpa armigera." Insect Biochemistry and Molecular Biology 122, no. : 103387.

Review
Published: 27 February 2020 in Pest Management Science
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Vip3A proteins are widely used for controlling pest Lepidoptera. Different binding sites with different receptors in the insect midgut membrane and lack of cross‐resistance with crystal (Cry) proteins enhance their applicability, as both single proteins and proteins pyramided with Cry proteins in transgenic Bt crops. Vip3A proteins are effective but there is relatively little information about their structure, function, activation, specificity, and mode of action. In addition, the mechanism of insect resistance to these proteins is unknown. Phylogenetic analysis and multiple sequence alignment showed that Vip3A proteins are genetically distant from Cry proteins. The mode of action and insecticidal activity of Vip3A proteins are discussed in this review. This review also provides detailed information about the Vip3A protein family that may aid in the design of more efficient pest management strategies in response to insect resistance to insecticidal proteins. © 2020 Society of Chemical Industry

ACS Style

Swapan Chakrabarty; Minghui Jin; Chao Wu; Panchali Chakraborty; Yutao Xiao. Bacillus thuringiensis vegetative insecticidal protein family Vip3A and mode of action against pest Lepidoptera. Pest Management Science 2020, 76, 1612 -1617.

AMA Style

Swapan Chakrabarty, Minghui Jin, Chao Wu, Panchali Chakraborty, Yutao Xiao. Bacillus thuringiensis vegetative insecticidal protein family Vip3A and mode of action against pest Lepidoptera. Pest Management Science. 2020; 76 (5):1612-1617.

Chicago/Turabian Style

Swapan Chakrabarty; Minghui Jin; Chao Wu; Panchali Chakraborty; Yutao Xiao. 2020. "Bacillus thuringiensis vegetative insecticidal protein family Vip3A and mode of action against pest Lepidoptera." Pest Management Science 76, no. 5: 1612-1617.

Journal article
Published: 28 August 2019 in Biofuels
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ACS Style

S. Chakrabarty; A. K. M. Aminul Islam; Nasrin Sultana; P. Chakraborty. Genetic diversity of Jatropha curcas L. genotypes: a potential biofuel crop in Bangladesh. Biofuels 2019, 1 -9.

AMA Style

S. Chakrabarty, A. K. M. Aminul Islam, Nasrin Sultana, P. Chakraborty. Genetic diversity of Jatropha curcas L. genotypes: a potential biofuel crop in Bangladesh. Biofuels. 2019; ():1-9.

Chicago/Turabian Style

S. Chakrabarty; A. K. M. Aminul Islam; Nasrin Sultana; P. Chakraborty. 2019. "Genetic diversity of Jatropha curcas L. genotypes: a potential biofuel crop in Bangladesh." Biofuels , no. : 1-9.

Review
Published: 10 June 2019 in International Journal of Molecular Sciences
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ATP-binding cassette (ABC) transporters, a large class of transmembrane proteins, are widely found in organisms and play an important role in the transport of xenobiotics. Insect ABC transporters are involved in insecticide detoxification and Bacillus thuringiensis (Bt) toxin perforation. The complete ABC transporter is composed of two hydrophobic transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). Conformational changes that are needed for their action are mediated by ATP hydrolysis. According to the similarity among their sequences and organization of conserved ATP-binding cassette domains, insect ABC transporters have been divided into eight subfamilies (ABCA–ABCH). This review describes the functions and mechanisms of ABC transporters in insecticide detoxification, plant toxic secondary metabolites transport and insecticidal activity of Bt toxin. With improved understanding of the role and mechanisms of ABC transporter in resistance to insecticides and Bt toxins, we can identify valuable target sites for developing new strategies to control pests and manage resistance and achieve green pest control.

ACS Style

Chao Wu; Swapan Chakrabarty; Minghui Jin; Kaiyu Liu; Yutao Xiao. Insect ATP-Binding Cassette (ABC) Transporters: Roles in Xenobiotic Detoxification and Bt Insecticidal Activity. International Journal of Molecular Sciences 2019, 20, 2829 .

AMA Style

Chao Wu, Swapan Chakrabarty, Minghui Jin, Kaiyu Liu, Yutao Xiao. Insect ATP-Binding Cassette (ABC) Transporters: Roles in Xenobiotic Detoxification and Bt Insecticidal Activity. International Journal of Molecular Sciences. 2019; 20 (11):2829.

Chicago/Turabian Style

Chao Wu; Swapan Chakrabarty; Minghui Jin; Kaiyu Liu; Yutao Xiao. 2019. "Insect ATP-Binding Cassette (ABC) Transporters: Roles in Xenobiotic Detoxification and Bt Insecticidal Activity." International Journal of Molecular Sciences 20, no. 11: 2829.

Journal article
Published: 28 March 2019 in The Open Agriculture Journal
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Introduction: An experiment was conducted to study combining ability and heterosis for yield and related traits in chili during November 2015 to September 2017. Materials and Methods: The experimental material consisted of six parents and their fifteen F1’s developed by half diallel mating design. Analysis of variance for combining ability exhibited significant General and Specific Combining Ability (GCA and SCA) effects for all the characters studied. Results: The SCA variance was higher than GCA variance for all the traits except ten fresh fruit weight, fruit length and fruit width indicating the predominance of non-additive type of gene action. The parents P1 and P6 were identified as the best general combiners and the hybrids P1×P6, P1×P4 and P2×P5 were identified as the best specific combinations for fresh fruit yield per plant and related traits. The hybrids P1×P6, P1×P4 and P3×P6 showed significant average heterosis and heterobeltiosis for fresh fruit yield per plant and its related traits. (H1/D)0.5 ratio indicated partial dominance effect of genes for all the traits. Conclusion: Therefore, it may be possible to take advantages of better heterotic effects to be fixed in the later generations to facilitate further selection and best specific combinations for development of the hybrid variety of chili which can help to increase the total production in Bangladesh.

ACS Style

Swapan Chakrabarty; A. K. M. Aminul Islam; M. A. Khaleque Mian; Tofayel Ahamed. Combining Ability and Heterosis for Yield and Related Traits in Chili (Capsicum annuum L.). The Open Agriculture Journal 2019, 13, 34 -43.

AMA Style

Swapan Chakrabarty, A. K. M. Aminul Islam, M. A. Khaleque Mian, Tofayel Ahamed. Combining Ability and Heterosis for Yield and Related Traits in Chili (Capsicum annuum L.). The Open Agriculture Journal. 2019; 13 (1):34-43.

Chicago/Turabian Style

Swapan Chakrabarty; A. K. M. Aminul Islam; M. A. Khaleque Mian; Tofayel Ahamed. 2019. "Combining Ability and Heterosis for Yield and Related Traits in Chili (Capsicum annuum L.)." The Open Agriculture Journal 13, no. 1: 34-43.

Comparative study
Published: 02 February 2019 in Toxins
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Crystalline (Cry) proteins from Bacillus thuringiensis (Bt) are widely used in sprays and transgenic crops to control insect pests, but the evolution of insect resistance threatens their long-term use. Different resistance mechanisms have been identified, but some have not been completely elucidated. Here, the transcriptome of the midgut and proteome of the peritrophic matrix (PM) were comparatively analyzed to identify potential mechanism of resistance to Cry1Ac in laboratory-selected strain XJ10 of Helicoverpa armigera. This strain had a 146-fold resistance to Cry1Ac protoxin and 45-fold resistance to Cry1Ac activated toxin compared with XJ strain. The mRNA and protein levels for several trypsin genes were downregulated in XJ10 compared to the susceptible strain XJ. Furthermore, 215 proteins of the PM were identified, and nearly all had corresponding mRNAs in the midgut. These results provide new insights that the PM may participate in Bt resistance.

ACS Style

Minghui Jin; Chongyu Liao; Swapan Chakrabarty; Kongming Wu; Yutao Xiao. Comparative Proteomics of Peritrophic Matrix Provides an Insight into its Role in Cry1Ac Resistance of Cotton Bollworm Helicoverpa armigera. Toxins 2019, 11, 92 .

AMA Style

Minghui Jin, Chongyu Liao, Swapan Chakrabarty, Kongming Wu, Yutao Xiao. Comparative Proteomics of Peritrophic Matrix Provides an Insight into its Role in Cry1Ac Resistance of Cotton Bollworm Helicoverpa armigera. Toxins. 2019; 11 (2):92.

Chicago/Turabian Style

Minghui Jin; Chongyu Liao; Swapan Chakrabarty; Kongming Wu; Yutao Xiao. 2019. "Comparative Proteomics of Peritrophic Matrix Provides an Insight into its Role in Cry1Ac Resistance of Cotton Bollworm Helicoverpa armigera." Toxins 11, no. 2: 92.

Journal article
Published: 21 December 2018 in Pesticide Biochemistry and Physiology
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When any living organism is frequently exposed to any drugs or toxic substances, they evolve different detoxification mechanism to confront with toxicants during absorption and metabolism. Likewise, the insects have evolved detoxification mechanisms as they are frequently exposed to different toxic secondary plant metabolites and commercial insecticides. ABC transporter superfamily is one of the largest and ubiquitous group of proteins which play an important role in phase III of the detoxification process. However, knowledge about this gene family remains largely unknown. To help fill this gap, we have identified a total of 54 ABC transporters in the Helicoverpa armigera genome which are classified into eight subfamilies (A-H) by phylogenetic analysis. The temporal and spatial expression profiles of these 54 ABC transporters throughout H. armigera development stages and seven tissues and their responses to five different insecticides, were investigated using RNA-seq analysis. Furthermore, the mRNA expression of eight selected genes in different tissues and six genes responses to insecticides were confirmed by the quantitative real-time PCR (RT-qPCR). Moreover, H. armigera become more sensitive to abamectin and indoxacarb when P-gp was inhibited. These results provide a foundation for further studies of ABCs in H. armigera.

ACS Style

Minghui Jin; Chongyu Liao; Swapan Chakrabarty; Weigang Zheng; Kongming Wu; Yutao Xiao. Transcriptional response of ATP-binding cassette (ABC) transporters to insecticides in the cotton bollworm, Helicoverpa armigera. Pesticide Biochemistry and Physiology 2018, 154, 46 -59.

AMA Style

Minghui Jin, Chongyu Liao, Swapan Chakrabarty, Weigang Zheng, Kongming Wu, Yutao Xiao. Transcriptional response of ATP-binding cassette (ABC) transporters to insecticides in the cotton bollworm, Helicoverpa armigera. Pesticide Biochemistry and Physiology. 2018; 154 ():46-59.

Chicago/Turabian Style

Minghui Jin; Chongyu Liao; Swapan Chakrabarty; Weigang Zheng; Kongming Wu; Yutao Xiao. 2018. "Transcriptional response of ATP-binding cassette (ABC) transporters to insecticides in the cotton bollworm, Helicoverpa armigera." Pesticide Biochemistry and Physiology 154, no. : 46-59.

Book chapter
Published: 04 July 2018 in Advances in Biofuels and Bioenergy
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ACS Style

Sri Rizki Putri Primandari; A.K.M. Aminul Islam; Zahira Yaakob; Swapan Chakrabarty. Jatropha Curcas L. Biomass Waste and Its Utilization. Advances in Biofuels and Bioenergy 2018, 1 .

AMA Style

Sri Rizki Putri Primandari, A.K.M. Aminul Islam, Zahira Yaakob, Swapan Chakrabarty. Jatropha Curcas L. Biomass Waste and Its Utilization. Advances in Biofuels and Bioenergy. 2018; ():1.

Chicago/Turabian Style

Sri Rizki Putri Primandari; A.K.M. Aminul Islam; Zahira Yaakob; Swapan Chakrabarty. 2018. "Jatropha Curcas L. Biomass Waste and Its Utilization." Advances in Biofuels and Bioenergy , no. : 1.

Research article
Published: 12 June 2017 in Advances in Agriculture
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The present investigation was carried out to estimate the genetic variability and character association. Significant differences were observed among the genotypes for all the 15 traits for 20 chili genotypes. The highest genotypic coefficient of variation and phenotypic coefficient of variation were found for ten edible fruit weight, number of fruits per plant, fruit yield per plant, ten dry fruit weight, fruit length, fruit width, and weight of seeds per fruit. High heritability coupled with very high genetic advance as per cent of mean was observed for ten edible fruit weight, ten dry fruit weight, fruit length, number of fruits per plant, and fruit yield per plant. Phenotypic correlation coefficient among different traits indicated that fruit yield per plant at green stage had significant and positive association with ten edible green fruit weight, number of primary branches, harvest duration, and first fruit maturity and significant negative correlation with days to first picking. The path coefficient analysis revealed that days to first picking had the maximum positive direct effect on fruit yield per plant followed by harvest duration, ten edible fruit weight, ten dry fruit weight, hundred-seed weight, number of fruits per plant, first fruit maturity, and number of primary branches. Days to first picking had the maximum positive direct effect but significant negative effect with fruit yield per plant which indicated that the trait should be selected to expunge the undesirable indirect effect in order to make use of the direct effect. Therefore, selection should be practiced for ten edible fruit weight, ten dry fruit weight, number of fruits per plant, harvest duration, and hundred-seed weight for direct improvement of fruit yield per plant.

ACS Style

Swapan Chakrabarty; A. K. M. Aminul Islam. Selection Criteria for Improving Yield in Chili (Capsicum annuum). Advances in Agriculture 2017, 2017, 1 -9.

AMA Style

Swapan Chakrabarty, A. K. M. Aminul Islam. Selection Criteria for Improving Yield in Chili (Capsicum annuum). Advances in Agriculture. 2017; 2017 ():1-9.

Chicago/Turabian Style

Swapan Chakrabarty; A. K. M. Aminul Islam. 2017. "Selection Criteria for Improving Yield in Chili (Capsicum annuum)." Advances in Agriculture 2017, no. : 1-9.