This study presents the development and application of a textile finishing treatment with antimicrobial properties based on silver nanostructures. The methodology involved the initial synthesis of silver-supported structures on aluminosilicates, which were subsequently applied to 100% raw cotton Jersey fabric through an impregnation finishing process. The treated cotton samples were evaluated for antimicrobial activity using the Kirby-Bauer disk diffusion method. The tested bacterial strains included Gram-negative as Shigella sp., Pseudomonas sp. M13, Pseudomonas sp. M14, Pseudomonas putida KT2440, and Escherichia coli, as well as Gram-positive Staphylococcus aureus ATCC 29213 and Streptococcus agalactiae ATCC 12386 (American Type Culture Collection) all of which exhibited growth inhibition in the presence of the treated textile, demonstrating the effectiveness of the antimicrobial finish.