This study presents the development and application of a textile finishing treatment with antimicrobial properties based on silver nanostructures. The methodology involved the initial synthesis of silver-supported structures on aluminosilicates, which were subsequently applied to 100% raw cotton Jersey fabric through an impregnation finishing process. The treated cotton samples were evaluated for antimicrobial activity using the Kirby-Bauer disk diffusion method. The tested bacterial strains included Gram-negative as Shigella sp., Pseudomonas sp. M13, Pseudomonas sp. M14, Pseudomonas putida KT2440, and Escherichia coli, as well as Gram-positive Staphylococcus aureus ATCC 29213 and Streptococcus agalactiae ATCC 12386 (American Type Culture Collection) all of which exhibited growth inhibition in the presence of the treated textile, demonstrating the effectiveness of the antimicrobial finish.

The surface modification of dental implants with nanostructured films enables the development of the next generation of biomaterials that promote osseointegration. In this study, a uniform layer of titanium oxide nanotubes (TNTs) was successfully formed on a Ti-6Al-4V dental implant screw through anodic oxidation. TNTs were morphologically characterized by Scanning Electron Microscopy (SEM), obtaining dimensions of 64.88 ± 10 nm in diameter and 5.34 ± 5 µm in length. Additionally, a crystal size of 23.45 nm was determined by X-ray diffraction (XRD) analysis. The TNT layer on the dental implant screw was evaluated in an in vitro system in direct contact with human osteoblast cells (hFOB) for 24 h and 48 h, finding cell growth near to the screw threads. Further, the biocompatibility of the dental screw coated with TNTs was evaluated using a flow cytometric assay with 7-AAD, demonstrating that cell viability was not affected at 24 h and 48 h. This study opens the perspective of the study of inflammation and osseointegration induced by implants coated with TNTs.

Conventional physical sunscreens are formulated with titanium oxide (TiO ₂ ), which reflects and scatters the UVA and UVB radiation, making them suitable for sensitive skin. However, its high refractive index can result in an undesirable white cast and potentially limit their cosmetic acceptability and effectiveness. Therefore, this study focuses on the formation of titanium oxide nanotubes (TNTs) as an alternative, since their nanoscale size minimizes light scattering and allows for a more transparent appearance when applied to the skin. TNTs were formed by anodic oxidation using an electrolyte based on ethylene glycol (EG), ammonium fluoride (NH ₄ F), and distilled water. Anodization was conducted at a constant voltage of 60 V for 1 h. TNTs were characterized by X-ray diffraction (XRD) and transmission electron microscope (TEM), which confirmed the presence of Ti ₂ O and an inner diameter of 53 ± 4 nm. Biocompatibility was assessed using 3D spheroid cultures of hFOB 1.19 osteoblasts, and results showed that TNTs at concentrations of 0.2 mg/mL and 0.02 mg/mL were non-cytotoxic. The 0.2 mg/mL concentration exhibited a superior photoprotective effect, maintaining approximately 75% cell viability under UVB radiation conditions. These findings highlight the potential of TNTs as transparent, biocompatible UV filters for next-generation sunscreens.

This work presents the design, manufacturing, and implementation of three uniaxial rotational molding (URM) systems, each corresponding to a mold of the trachea, lung, and tumor lesions, for the production of a soft anthropomorphic phantom. The phantom structures were manufactured using Ecoflex® 00-20 silicone. Each mold was designed in Autodesk Inventor and fabricated via 3D printing, enabling customized and reproducible geometries. A proportional-derivative (PD) controller was implemented in each URM system to regulate rotation speed according to a reference trajectory. Additionally, curing times and silicone volumes were tested to ensure uniform material distribution. The resulting phantom elements demonstrate the potential of controlled rotational molding for producing soft, anatomically realistic models suitable for radiopharmaceutical characterization, medical research, and quality assurance in nuclear imaging systems.

The γδ T cells belong to a subgroup of T cells known as non-conventional T cells due to their limited T cell receptor (TCR) repertoire and ability to recognize non-peptide antigens. They play a crucial role in combating infections and tumors. Vγ9Vδ2 T cells are typically activated by molecules containing diphosphate groups, collectively known as phosphoantigens (pAgs), through a non-canonical mechanism which involves the intracellular domain of butyrofilin (BTN)3A1 protein. However, no FDA-approved drugs have yet been shown to activate them, and the underlying cellular mechanisms remain unknown. In this study, we combined high-throughput virtual screening of an FDA-approved drug database with in vitro cellular assays to identify potential γδ T cells activators. Our findings demonstrate that Nitazoxanide (NTZ) and Tinidazole induce moderate elicited a statistically significant increase in interferon (IFN)-γ production of Vγ9Vδ2 T cells by their probably interaction with the pAg binding site of BTN3A1. Additionally, NTZ induces expression of CD107a, but only at the highest concentrations tested and promotes the upregulation of HLA-DR in total PBMCs and CD14⁺ monocytes. Blocking BTN3A with a specific antibody led to a marked reduction in all NTZ-induced activations. This work identifies NTZ as a previously unrecognized activator of γδ T cells, highlighting its immunomodulatory potential beyond its known clinical uses. These findings broaden our understanding of γδ T cells pharmacology and suggest new opportunities for drug repurposing and the design of novel chemical scaffolds. Further mechanistic studies will be essential to fully define how NTZ engages the BTN3A–γδ T cells axis.