Crocin is a bioactive compound that naturally occurs in some medicinal plants, especially saffron and gardenia fruit. Different conventional and novel methods are used for its extraction. Due to some control conditions, recent methods such as ultrasonic extraction, supercritical fluid extraction, enzyme-associated extraction, microwave extraction, and pulsed electric field extraction are widely used because these methods give more yield and efficiency. Crocin is incorporated into different food products to make functional foods. However, it can also aid in the stability of food products. Due to its ability to protect against brain diseases, the demand for crocin has been rising in the pharmaceutical industry. It also contain antioxidant, anti-inflammatory, anticancer and antidepressant qualities. This review aims to describe crocin and its role in developing functional food, extraction, and bioavailability in various brain-related diseases. The results of the literature strongly support the importance of crocin against various diseases and its use in making different functional foods.

This study aimed to investigate the effect of sesamol (SEM) on the protein kinase A (PKA) pathway in obesity-related hepatic steatosis treatment by using high-fat diet (HFD)-induced obese mice and a palmitic acid (PA)-treated HepG2 cell line. SEM reduced the body weight gain of obese mice and alleviated related metabolic disorders such as insulin resistance, hyperlipidemia, and systemic inflammation. Furthermore, lipid accumulation in the liver and HepG2 cells was reduced by SEM. SEM downregulated the gene and protein levels of lipogenic regulator factors, and upregulated the gene and protein levels of the regulator factors responsible for lipolysis and fatty acid β-oxidation. Meanwhile, SEM activated AMP-activated protein kinase (AMPK), which might explain the regulatory effect of SEM on fatty acid β-oxidation and lipogenesis. Additionally, the PKA-C and phospho-PKA substrate levels were higher after SEM treatment. Further research found that after pretreatment with the PKA inhibitor, H89, lipid accumulation was increased even with SEM administration in HepG2 cells, and the effect of SEM on lipid metabolism-related regulator factors was abolished by H89. In conclusion, SEM has a positive therapeutic effect on obesity and obesity-related hepatic steatosis by regulating the hepatic lipid metabolism mediated by the PKA pathway.

In this study, we established a rapid and sensitive method for the detection of viable Salmonella Typhimurium, Staphylococcus aureus, and Listeria monocytogenes in milk using biotin-exposure-based immunomagnetic separation (IMS) combined with sodium dodecyl sulfate (SDS), propidium monoazide (PMA), and multiplex real-time PCR (mRT-PCR). We used IMS to lessen the assay time for isolation of target bacteria. We then optimized the coupling conditions and immunomagnetic capture process. The immunoreaction and incubation times for 5 μg of mAb coupled with 500 μg of streptavidin-functionalized magnetic beads using a streptavidin-biotin system were 90 and 30 min, respectively. Treatment with SDS-PMA before mRT-PCR amplification eliminated false-positive outcomes from dead bacteria and identified viable target bacteria with good sensitivity and specificity. The limit of detection of IMS combined with the SDS-PMA-mRT-PCR assay for the detection of viable Salmonella Typhimurium, Staph. aureus, and L. monocytogenes in spiked milk matrix samples was 10 cfu/mL and remained significant even in the appearance of 106 cfu/mL of nontarget bacteria. The entire detection process was able to identify viable bacteria within 9 h. The combination of biotin-exposure-mediated IMS and SDS-PMA-mRT-PCR has potential value for the rapid and sensitive detection of foodborne pathogens.

As critical urban blue–green infrastructure, urban wetland parks serve as vital venues for visitors to obtain restorative experiences. However, existing studies primarily emphasize their ecological and economic benefits, with comparatively limited attention paid to their roles in promoting public mental health and enhancing well-being. Using Yanghu Wetland Park in Changsha as a case study, this research investigates how restorative environmental perception influences visitors’ well-being through two mediating variables: environmental preference and place attachment. A total of 251 valid responses were collected through field surveys and questionnaires. Structural equation modeling (SEM) was employed to empirically examine the relationships among the variables. This study enriches the theoretical framework of environmental psychology and urban landscape behavior research. It also provides evidence-based insights into the planning and design of urban wetland parks, contributing to the enhancement of public well-being and overall life satisfaction.

China’s urban–rural income gap has narrowed in objective terms, yet rural households may continue to perceive substantial income inequality. Existing research often focuses on income levels; it remains unclear whether the source and institutional form of income shape perceived rural–urban income inequality among households at similar income levels. This study examines whether land transfer income, cooperative membership, enterprise cooperation, and non-farm employment stability are related to perceived rural–urban income inequality. Land transfer income is a binary indicator of whether household property income includes rental or other compensation income from transferring farmland management rights, and organized agricultural operation refers to cooperative membership or contractual enterprise cooperation. Using data from a 2025 survey of rural households in Hunan Province, this study estimates ordered logit models with county-clustered robust standard errors. Land transfer income, cooperative membership, and enterprise cooperation showed negative conditional relationships with perceived inequality, whereas having no fixed job showed the strongest positive relationship. The source-and-organization variables jointly improved explanatory performance beyond household income level (p < 0.001), and the core directions remained stable in robustness checks. Agricultural income change and agricultural subsidies are examined only as exploratory extensions. The findings offer potential implications for farmland transfer institutions, producer organizations, and rural employment policy, rather than causal policy prescriptions.